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OfflineFrommer
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Registered: 02/08/16
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Loc: Amazon Jungle
Last seen: 6 years, 9 months
1st time isolation to bulk grow log
    #23450522 - 07/17/16 05:06 PM (7 years, 6 months ago)

Howdey folk

So finding all the tek needed in my location has taken what feels to be decades.
Learning how to cultivate has been a journey to say the least.

But here we go 9 months since starting and the funs begun.

I started off with a PE dish that had about 4 sectors, and worked it to this guy.



I then got some honey tek going and ended up with these.



I squirted some into a few grain jars, yesterday one looked like this.



I gave them the final shake, now I have these.



As for the rest now we wait and see..


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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23474037 - 07/24/16 11:14 PM (7 years, 6 months ago)

Update.

So the day has arrived, spawn check, tub check, sub check.
The mixing will comence once the sub has cooled off.



And the wait continues....


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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23491841 - 07/30/16 08:38 AM (7 years, 5 months ago)

Well 6 days in, couldnt resist a little preview.
Things seem to be moving along well, Im happy.



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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
Posts: 101
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23500697 - 08/02/16 05:05 AM (7 years, 5 months ago)

I was going to hold off the update, but moving things around and had a peak.
Yesterday's snap, Myc has gone wild.



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OfflineHugh Jorgan
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23500981 - 08/02/16 08:01 AM (7 years, 5 months ago)

Dont be surprised if the PE takes a while to fruit. Most cubes tend to fruit fairly quickly after consolidation of the spawn. PE is known to take a while (not always but often). Otherwise you're doing great. Have fun and enjoy.





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OfflinePsykronik
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Re: 1st time isolation to bulk grow log [Re: Hugh Jorgan]
    #23501130 - 08/02/16 08:55 AM (7 years, 5 months ago)

Wow nice one man, I'm working on my own isolate right now. 4th transfer down seems to be looking good. Did you test your culture or did you go straight to a monotub?


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OfflineHugh Jorgan
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Re: 1st time isolation to bulk grow log [Re: Psykronik]
    #23501325 - 08/02/16 10:15 AM (7 years, 5 months ago)

spores to agar. A couple of transfers to make sure its clean. then I go to a tub. usually after that I start cloning tissue to agar from my favorite clusters or fruits.



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Always go forward! Never go straight!!




Edited by Hugh Jorgan (08/02/16 10:16 AM)


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OfflinePsykronik
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Re: 1st time isolation to bulk grow log [Re: Hugh Jorgan]
    #23501347 - 08/02/16 10:23 AM (7 years, 5 months ago)

I meant does OP or anyone else for that matter do like a spawn to test tray to see if the isolate will fruit at all or just go all out and spawn directly to a bulk mono and take the risk of isolate not being s fruiter?


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Invisiblemupetmower
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Re: 1st time isolation to bulk grow log [Re: Psykronik]
    #23501352 - 08/02/16 10:25 AM (7 years, 5 months ago)

people do/have done both.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineMycologist217
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Re: 1st time isolation to bulk grow log [Re: mupetmower]
    #23501392 - 08/02/16 10:41 AM (7 years, 5 months ago)

The spores that are sold by vendors have been so thoroughly artificially selected that non-fruiting genetics are extremely extremely rare.

In my opinion of you aren't willing to test out an isolate culture for fruiting potential/colonization speed, you should isolate away from a clone sample....at least then you have a good idea of what kind of phenotypes make up the multi strain grow...not to mention in MS grows a type of horizontal gene transfer occurs called anastomosis, when this happens fungus cells anchor themselves to each other via tubules and the genetics transfer, narrowing down genetic variation in MS grows.


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OfflinePsykronik
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Re: 1st time isolation to bulk grow log [Re: Mycologist217]
    #23501438 - 08/02/16 10:57 AM (7 years, 5 months ago)

I think I will test my isolate out first. Got 8 grain jars sitting ready to go so I'll test out on one of those once my culture grows out a little more. It's hard to tell if it's finished sectoring at the moment. thanks for the info  :thanx:
Sorry for thread hijack OP  :jacked:


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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Psykronik]
    #23504751 - 08/03/16 08:35 AM (7 years, 5 months ago)

Ok so a few things to cover here.

1) Update. Here's a pic of day 10.
  Im very excited!!



Now to everyone's comments in order.

@ Mikehalb - This PE is a slow ass been testing isolates as I go, Spot on!

@ Psykronik - I have had this culture on PDA for +- 7 months, old plates that I isolate from I have been dropping into grain so I have a few attempts behind me on this culture just not the isolation. I have an idea of what to expect here, Also the original culture was from a highly skilled and exp. grower...


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23511554 - 08/05/16 02:24 AM (7 years, 5 months ago)

Well day 12 a little patch left to col, I hope the tub will be ready to birth in a week or so.
Lets see how knotting goes.



Quick edit and add, bit less crappy.



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Edited by Frommer (08/05/16 04:47 AM)


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23525098 - 08/09/16 02:55 PM (7 years, 5 months ago)

Sadly no pics for this update, ended up in hospital.
Hopefully Ill come home to a pinset for you all!!!


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OfflineHugh Jorgan
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Registered: 09/21/15
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23531535 - 08/11/16 01:06 PM (7 years, 5 months ago)

Just take care of yourself. You can always grow more mushies. You cant grow another you (yet).


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Hugh Jorgan]
    #23532205 - 08/11/16 05:03 PM (7 years, 5 months ago)

Thanks mate


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23532216 - 08/11/16 05:07 PM (7 years, 5 months ago)

Well here we go latest update, my ass is out of hospital.
And its a good thing I did PE on this run, slow asss hell.



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OfflinePsilocyBen17
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Registered: 10/20/13
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23532524 - 08/11/16 07:09 PM (7 years, 5 months ago)

Hey Fromer,

I'm very impressed that you took the time to do some research before starting out...more people around here need to do that!

With that said I feel I should point out that your spawn looks bacterial, as does your tub....if it does fruit you will see diminished yeilds. Don't get discouraged though! you have made a lot of progress in just a short time.

May I ask why you decided to make LC from a clean wedge? You'd have a better chance of developing clean spawn if you went directly from agar>grain...

Everyone talks about LC's like they are a piece of cake. Getting a CLEAN LC is a whole different story....they are risky and you need to have the proper filters to increase your chances of success...Once they get the hang of agar going LC's become obsolete to many people.

btw what is it that your agar wedge is growing on? Looks strange to me, but the growth looks fine.

Pb


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: PsilocyBen17]
    #23533654 - 08/12/16 04:39 AM (7 years, 5 months ago)

Dang it, FFS really thought I was nailing this tub.
The reason I went LC was my wedges often go green in the jar when they dry.
Myself and a mate keep hitting trich and shit, one article I read said the PDA often turns shitty due to the nutes and gos off creating contams.
My PDA in this snap is a pre-mixed solution, its the Oxoid Formulation.
Thats poured into normal disposable dishes, most tek I have to go to huge efforts for.
So I was not able to get Parafilm for a long time and my dishes where getting contams.
I found a paper tape that lets some air in and keeps the contams out so I seal the dish with that.


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23575487 - 08/25/16 09:41 AM (7 years, 5 months ago)

Well despite being bacterial, this looks a lot like my last attempt.
Blobs are starting up, last batch of blobs hit harder than double drops on tabs..
I had about 120 G wet on the last blobs round.



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OfflineKenetic
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23575497 - 08/25/16 09:44 AM (7 years, 5 months ago)

Quote:

Frommer said:
I was going to hold off the update, but moving things around and had a peak.
Yesterday's snap, Myc has gone wild.







Holy shit did you chew holes in your tub?  Lol


--------------------
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                                                :cookiemonster::elmo:



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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
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Re: 1st time isolation to bulk grow log [Re: Kenetic]
    #23616982 - 09/06/16 04:26 AM (7 years, 4 months ago)

Ok So been a while but things are slow, let's see if someone can see what the fuck I'm doing wrong.

So after about 3 weeks the blobs finally happen..





After pulling most the blobs some pins.




This shit looks healthy why is fruiting such a mission, I have trays not PE other cultures all doing this. Col 100% and slow fruiting..


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Offlinespore-ty
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Re: 1st time isolation to bulk grow log [Re: Kenetic]
    #23617053 - 09/06/16 05:46 AM (7 years, 4 months ago)

Quote:

kenetic said:
Quote:

Frommer said:
I was going to hold off the update, but moving things around and had a peak.
Yesterday's snap, Myc has gone wild.







Holy shit did you chew holes in your tub?  Lol




Lmao they do look a tad suspicious


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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: spore-ty]
    #23617058 - 09/06/16 05:50 AM (7 years, 4 months ago)

Quote:

spore-ty said:
Quote:

kenetic said:
Quote:

Frommer said:
I was going to hold off the update, but moving things around and had a peak.
Yesterday's snap, Myc has gone wild.







Holy shit did you chew holes in your tub?  Lol




Lmao they do look a tad suspicious




Ok for everyone RE my holes..

The plastic here is very brittle, when using a cam cutter the plastic shatters and cracks. So I took a soldering iron and melted holes as the tubs just won't cut well.


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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23624675 - 09/08/16 03:59 AM (7 years, 4 months ago)

Well I have some pins.



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OfflineRosie
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23624754 - 09/08/16 05:04 AM (7 years, 4 months ago)

Nice!


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OfflineFrommer
Feeling jiggly the stripper up.
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Re: 1st time isolation to bulk grow log [Re: Rosie]
    #23633929 - 09/10/16 08:57 PM (7 years, 4 months ago)

Well it seems I got lucky.

So the tub had a flush, Ive picked half and misted the sub as it was looking a bit dry.
Will pick the rest shortly and dunk then hope for the best..



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OfflineKenetic
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23634336 - 09/10/16 11:08 PM (7 years, 4 months ago)

That's a pretty weak culture.  good job isolating though


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleBoogieman47
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Re: 1st time isolation to bulk grow log [Re: Kenetic]
    #23634346 - 09/10/16 11:11 PM (7 years, 4 months ago)

If it was an isolate wouldn't it be the same fruit  like all grow look the same ?


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OfflineFrommer
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23643852 - 09/14/16 05:29 AM (7 years, 4 months ago)

Well I dunked the Sub to see what else would come.

Not a super culture, But for a 1st attempt at isolating a culture and 2nd Mono some good learning.

Any how current growth is not much more spectacular that the last harvest.
So far sitting on 80G wet on 950 grams of grain to a equal amount of 50/50.

Wonder if the leaving the blobs to get big hurt my 1st flush???? Anyone??



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OfflineFrommer
Feeling jiggly the stripper up.
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Registered: 02/08/16
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Re: 1st time isolation to bulk grow log [Re: Boogieman47]
    #23643858 - 09/14/16 05:32 AM (7 years, 4 months ago)

Hey noob47

When I say isolate this is not a true isolate of a single culture.
That said it is not the same mixed growth I started with.
So no not a true isolate, but yes a isolation of uniform growth.:confused:
Bit of a grey area.. Or maybe I need a new term??


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InvisibleBoogieman47
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Re: 1st time isolation to bulk grow log [Re: Frommer]
    #23644267 - 09/14/16 10:14 AM (7 years, 4 months ago)

I think you're good it does say isolation and not isolate if you said that in the op I didn't read it I just was checking the pics... I have some clones coming in now I just did 4 or 5 transfers then to grain honestly I don't think I'll waste much time trying to get an isolate if I get one then I do well keep it up man


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OfflineFrommer
Feeling jiggly the stripper up.
Male


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
Re: 1st time isolation to bulk grow log [Re: Boogieman47]
    #23667056 - 09/22/16 02:49 AM (7 years, 4 months ago)

Well guys, The second flush is done.

The 1st came in at 16G dry, the second looks about the same amount.
It would seem this will be about a 30G tub, That is now looking done and dusted.

On that note I close the grow log with some pics of flush 2, and dried 1st flush.
Some of the harvest is not in the pics, but imagination pays off.

Comments are welcome.

peace out.



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