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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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1st time isolation to bulk grow log
#23450522 - 07/17/16 05:06 PM (7 years, 6 months ago) |
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Howdey folk
So finding all the tek needed in my location has taken what feels to be decades. Learning how to cultivate has been a journey to say the least.
But here we go 9 months since starting and the funs begun.
I started off with a PE dish that had about 4 sectors, and worked it to this guy.

I then got some honey tek going and ended up with these.

I squirted some into a few grain jars, yesterday one looked like this.

I gave them the final shake, now I have these.

As for the rest now we wait and see..
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23474037 - 07/24/16 11:14 PM (7 years, 6 months ago) |
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Update.
So the day has arrived, spawn check, tub check, sub check. The mixing will comence once the sub has cooled off.

And the wait continues....
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23491841 - 07/30/16 08:38 AM (7 years, 5 months ago) |
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Well 6 days in, couldnt resist a little preview. Things seem to be moving along well, Im happy.
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23500697 - 08/02/16 05:05 AM (7 years, 5 months ago) |
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I was going to hold off the update, but moving things around and had a peak. Yesterday's snap, Myc has gone wild.
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Hugh Jorgan
Mycophile



Registered: 09/21/15
Posts: 173
Last seen: 2 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23500981 - 08/02/16 08:01 AM (7 years, 5 months ago) |
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Dont be surprised if the PE takes a while to fruit. Most cubes tend to fruit fairly quickly after consolidation of the spawn. PE is known to take a while (not always but often). Otherwise you're doing great. Have fun and enjoy.
 
-------------------- Always go forward! Never go straight!!
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Psykronik
OverGrower



Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: 1st time isolation to bulk grow log [Re: Hugh Jorgan]
#23501130 - 08/02/16 08:55 AM (7 years, 5 months ago) |
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Wow nice one man, I'm working on my own isolate right now. 4th transfer down seems to be looking good. Did you test your culture or did you go straight to a monotub?
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Hugh Jorgan
Mycophile



Registered: 09/21/15
Posts: 173
Last seen: 2 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Psykronik]
#23501325 - 08/02/16 10:15 AM (7 years, 5 months ago) |
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spores to agar. A couple of transfers to make sure its clean. then I go to a tub. usually after that I start cloning tissue to agar from my favorite clusters or fruits.
 
-------------------- Always go forward! Never go straight!!
Edited by Hugh Jorgan (08/02/16 10:16 AM)
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Psykronik
OverGrower



Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: 1st time isolation to bulk grow log [Re: Hugh Jorgan]
#23501347 - 08/02/16 10:23 AM (7 years, 5 months ago) |
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I meant does OP or anyone else for that matter do like a spawn to test tray to see if the isolate will fruit at all or just go all out and spawn directly to a bulk mono and take the risk of isolate not being s fruiter?
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: 1st time isolation to bulk grow log [Re: Psykronik]
#23501352 - 08/02/16 10:25 AM (7 years, 5 months ago) |
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people do/have done both.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 12 hours
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Re: 1st time isolation to bulk grow log [Re: mupetmower]
#23501392 - 08/02/16 10:41 AM (7 years, 5 months ago) |
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The spores that are sold by vendors have been so thoroughly artificially selected that non-fruiting genetics are extremely extremely rare.
In my opinion of you aren't willing to test out an isolate culture for fruiting potential/colonization speed, you should isolate away from a clone sample....at least then you have a good idea of what kind of phenotypes make up the multi strain grow...not to mention in MS grows a type of horizontal gene transfer occurs called anastomosis, when this happens fungus cells anchor themselves to each other via tubules and the genetics transfer, narrowing down genetic variation in MS grows.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Psykronik
OverGrower



Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
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Re: 1st time isolation to bulk grow log [Re: Mycologist217]
#23501438 - 08/02/16 10:57 AM (7 years, 5 months ago) |
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I think I will test my isolate out first. Got 8 grain jars sitting ready to go so I'll test out on one of those once my culture grows out a little more. It's hard to tell if it's finished sectoring at the moment. thanks for the info Sorry for thread hijack OP
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Psykronik]
#23504751 - 08/03/16 08:35 AM (7 years, 5 months ago) |
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Ok so a few things to cover here.
1) Update. Here's a pic of day 10. Im very excited!!

Now to everyone's comments in order.
@ Mikehalb - This PE is a slow ass been testing isolates as I go, Spot on!
@ Psykronik - I have had this culture on PDA for +- 7 months, old plates that I isolate from I have been dropping into grain so I have a few attempts behind me on this culture just not the isolation. I have an idea of what to expect here, Also the original culture was from a highly skilled and exp. grower...
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23511554 - 08/05/16 02:24 AM (7 years, 5 months ago) |
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Well day 12 a little patch left to col, I hope the tub will be ready to birth in a week or so. Lets see how knotting goes.

Quick edit and add, bit less crappy.
Edited by Frommer (08/05/16 04:47 AM)
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23525098 - 08/09/16 02:55 PM (7 years, 5 months ago) |
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Sadly no pics for this update, ended up in hospital. Hopefully Ill come home to a pinset for you all!!!
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Hugh Jorgan
Mycophile



Registered: 09/21/15
Posts: 173
Last seen: 2 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23531535 - 08/11/16 01:06 PM (7 years, 5 months ago) |
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Just take care of yourself. You can always grow more mushies. You cant grow another you (yet).
-------------------- Always go forward! Never go straight!!
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Hugh Jorgan]
#23532205 - 08/11/16 05:03 PM (7 years, 5 months ago) |
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Thanks mate
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23532216 - 08/11/16 05:07 PM (7 years, 5 months ago) |
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Well here we go latest update, my ass is out of hospital. And its a good thing I did PE on this run, slow asss hell.
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23532524 - 08/11/16 07:09 PM (7 years, 5 months ago) |
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Hey Fromer,
I'm very impressed that you took the time to do some research before starting out...more people around here need to do that!
With that said I feel I should point out that your spawn looks bacterial, as does your tub....if it does fruit you will see diminished yeilds. Don't get discouraged though! you have made a lot of progress in just a short time.
May I ask why you decided to make LC from a clean wedge? You'd have a better chance of developing clean spawn if you went directly from agar>grain...
Everyone talks about LC's like they are a piece of cake. Getting a CLEAN LC is a whole different story....they are risky and you need to have the proper filters to increase your chances of success...Once they get the hang of agar going LC's become obsolete to many people.
btw what is it that your agar wedge is growing on? Looks strange to me, but the growth looks fine.
Pb
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: PsilocyBen17]
#23533654 - 08/12/16 04:39 AM (7 years, 5 months ago) |
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Dang it, FFS really thought I was nailing this tub. The reason I went LC was my wedges often go green in the jar when they dry. Myself and a mate keep hitting trich and shit, one article I read said the PDA often turns shitty due to the nutes and gos off creating contams. My PDA in this snap is a pre-mixed solution, its the Oxoid Formulation. Thats poured into normal disposable dishes, most tek I have to go to huge efforts for. So I was not able to get Parafilm for a long time and my dishes where getting contams. I found a paper tape that lets some air in and keeps the contams out so I seal the dish with that.
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Frommer
Feeling jiggly the stripper up.



Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 1st time isolation to bulk grow log [Re: Frommer]
#23575487 - 08/25/16 09:41 AM (7 years, 5 months ago) |
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Well despite being bacterial, this looks a lot like my last attempt. Blobs are starting up, last batch of blobs hit harder than double drops on tabs.. I had about 120 G wet on the last blobs round.
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