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Offlinemaestro50
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Agar question
    #23448880 - 07/17/16 06:01 AM (7 years, 6 months ago)

Hello all!

Im thinking of getting into agar, and i am reading alot. But one thing is confusing for me..

The purpose of agar is to keep the good mycelium for good growth. And ive seen people cultivate agar with drops from syringe. How would i obtain a good spore? Shouldnt i first try to grow them and then take a tiny piece from inside then put it into petri dish... My question is how to obtain good mycelium or general shroom media to put into agar?


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Invisibleweetsie
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Re: Agar question [Re: maestro50]
    #23448900 - 07/17/16 06:14 AM (7 years, 6 months ago)

Agar is often about clean mycelium.

spores>grain

You get everything that was in the syringe/print growing along side your mushroom mycelium.

spores>agar>grain

Many times mycelium will outrun contaminants that were in with the spores, bacteria is very slow to move across an agar plate so taking mycelium from the edge of the growth means you're not inoculating your grain with it.

Quote:

Shouldnt i first try to grow them and then take a tiny piece from inside then put it into petri dish... My question is how to obtain good mycelium or general shroom media to put into agar?




No. If you're starting from spores, put them on agar, select aggressive and clean mushroom mycelium to transfer to your grain. After you get fruits then of course cloning on agar for your next grow is a good way to go.

Basically everything starts on agar, If I use spores they go on agar, If I clone I clone on agar, if someone sends me an edible wedge of agar, it goes on agar first to make sure it's clean.


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Offlinemaestro50
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Re: Agar question [Re: weetsie]
    #23450526 - 07/17/16 05:08 PM (7 years, 6 months ago)

Thanks man and i have few more questions. agar really seems interesting and also funn to do. and ofcourse is next step in growing beauties.

First, what do u mean by select aggressive and clean mushroom mycelium to transfer to grain? I get the spore print, then i apply a bit of it to agar agar and in the same time i grow the mushroom to check if the potency is good? Then if its good i have that spore in agar developing, and then take good white stuff from the edge right?


Really starting this so noob :crazy2:


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Re: Agar question [Re: maestro50] * 1
    #23450987 - 07/17/16 07:52 PM (7 years, 6 months ago)

this thread is literally right above yours. read that, as well as lot of other teks/info on agar.

Pasty Plates is great, too.

there are 100's of them.

Trusted Cultivator's agar links too.


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Edited by mupetmower (07/17/16 07:53 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Agar question [Re: mupetmower]
    #23451035 - 07/17/16 08:05 PM (7 years, 6 months ago)

:whathesaid:

Last link in my signature


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Invisibleweetsie
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Re: Agar question [Re: maestro50]
    #23451122 - 07/17/16 08:33 PM (7 years, 6 months ago)

Quote:

maestro50 said:
First, what do u mean by select aggressive and clean mushroom mycelium to transfer to grain?





When you put spores on agar you will get potentially thousands of strains growing, some of it will appear as fast rhizomorphic mycelium and some will be slow and weak looking. Selecting the former guarantees you nothing but can certainly improve your odds of success.

Quote:

maestro50 said:
I get the spore print, then i apply a bit of it to agar agar and in the same time i grow the mushroom to check if the potency is good?





No, every spore print will contain thousands of spores which will make thousands of strains. Most of them will be okay, some will be useless and some will be amazing.

Using the print and getting lucky will a good yield of potent shrooms means very little, the next time you use that print you could get barely active junk.

Watch RR's agar videos, they make it clearer than I will ever be able to explain.


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Offlinemaestro50
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Re: Agar question [Re: mupetmower]
    #23453732 - 07/18/16 05:34 PM (7 years, 6 months ago)

I know and i read. Everybody read i guess, but the specific question is good to ask and is always nice to get answer u want... Some details are not always said... :tongue:


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Offlinemaestro50
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Re: Agar question [Re: weetsie]
    #23453737 - 07/18/16 05:36 PM (7 years, 6 months ago)

Quote:

maestro50 said:
I get the spore print, then i apply a bit of it to agar agar and in the same time i grow the mushroom to check if the potency is good?





No, every spore print will contain thousands of spores which will make thousands of strains. Most of them will be okay, some will be useless and some will be amazing.

Using the print and getting lucky will a good yield of potent shrooms means very little, the next time you use that print you could get barely active junk.

Watch RR's agar videos, they make it clearer than I will ever be able to explain.




So how do i select the best spore? dont understand at all how to cultivate the best spore from the print on agar

U said i DONT put a little spore on agar? from where do i get mycelium than. pls help confused hehe


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Re: Agar question [Re: maestro50]
    #23453756 - 07/18/16 05:44 PM (7 years, 6 months ago)



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Re: Agar question [Re: maestro50]
    #23453769 - 07/18/16 05:48 PM (7 years, 6 months ago)

Use the agar to find healthy strong growth. You can then either continue to transfer until you have an isolate or do an ms grow. If you grow from ms you can then clone any fruits that look good.

Once clones or isolates are obtained you then do a test grow of each to see if it's got everything you want, potency, yield, etc. You must do a test grow, simply having a clone or an isolate is guarantee of anything (though with a clone at least you know it can fruit).
Most isolates tend to suck with 10% being really exceptional and 1% being stellar. Clones are a little more average but if you took 100 clones, for sure there would be 5 or 6 that were amazing.

Once you have your desired genetics you then slant the masters and grow the clone or isolate for the next 20 years. No more spores, unless you want to start over, then you simply take a print.


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Offlinemaestro50
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Re: Agar question [Re: Pastywhyte]
    #23455389 - 07/19/16 08:56 AM (7 years, 6 months ago)

Guys i appreciate your respond but u didnt answer my question again! If i dont understand this part my agar adventure will dissapear now...

Pasty u are talking about obtaining an extraordinary agar situation but again how do i get there. I asked u if u put a bit of spore print on agar and in the same time grow batch of same print so i can see the quality... and u said no. So STARTING from spore print... How do i make fertile agar culture? Ive read all links and searched bunch but still dont know this first basic step. helpzzzz


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Re: Agar question [Re: maestro50]
    #23455516 - 07/19/16 09:38 AM (7 years, 6 months ago)

What do you mean I said no? Either put a drop of spore solution or streak spores on the plate with a sterile loop or scalpel blade or something. Then let them germinate and grow. I'm not sure what else to tell you man.


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Re: Agar question [Re: Pastywhyte]
    #23455548 - 07/19/16 09:47 AM (7 years, 6 months ago)

i think he is asking if he can inoc some plates, as well as inoc some brf(or something) to grow and fruit along side the plates, to test their potency.

you can, but it's MS, which means you wont know for sure that the fruits will end up the same from the ones you grow to the ones you are making plates of. so what pasty is saying is to make the plates, grow them out, make transfers, picking the best growth, then eventually inoc with the plates to test the culture, while even keeping a master in case its an amazing culture.


Pasty can definitely explain better. i have a hard time getting what i mean out.


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Re: Agar question [Re: mupetmower]
    #23455565 - 07/19/16 09:53 AM (7 years, 6 months ago)

Okay if he is talking about knocking up something with the spores in the meantime then yeah why not? Just remember that contamination is a risk when you do that.

Potency is genetic. Spores are a genetic gamble. I can do 10 grows from spores and have different results every time.


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Offlinemaestro50
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Re: Agar question [Re: Pastywhyte]
    #23460323 - 07/20/16 06:10 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Okay if he is talking about knocking up something with the spores in the meantime then yeah why not? Just remember that contamination is a risk when you do that.

Potency is genetic. Spores are a genetic gamble. I can do 10 grows from spores and have different results every time.




Im still confused. More than before lol. Let me sum it up a bit.

So i have my agar agar ready. And i have spore print, or spore syringe. So what do i do now? Lets say i dont yet grow shrooms, BRF cakes. I only have my spores on agar and waiting for mycelium? If that is right, how do i cultivate the mycelium culture to reach the quality u are talking about?

Mupetmower said:

1make the plates- Make agar formula in plates... right?

2grow them out- Wait for mycelium to advace...right?

3make transfers, picking the best growth, then eventually inoc with the plates to test the culture, while even keeping a master in case its an amazing culture.- This i dont understand... Make transfer from one agar plate to another... What will i achieve with it? And ofcourse... what does, picking the best growth means... how do i spot the best myc? And lastly, what do u mean when u said inoc with the plates to test culture... Inoculate cakes with the whole agar plate with myc on, then growing them and testing?

Bare with me guys... I feel really bad, im feeling like bothering u but its really not clear to me. :sun:


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Re: Agar question [Re: maestro50]
    #23460351 - 07/20/16 06:18 PM (7 years, 6 months ago)

Okay here I will try once more.

Put spores on agar plate. Wait for growth.

Once you have growth cut a tiny rice sized piece (wedge) from the plate and put on a new plate. Wait for that to grow.

Observe new growth. If it looks clean you can then either transfer another piece to a new plate or several plates, or you can put a decent sized wedge into a grain jar.

Always choose healthy fast areas to cut your transfer pieces from. That will ensure the culture is the best available.


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Re: Agar question [Re: Pastywhyte]
    #23460718 - 07/20/16 08:53 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Observe new growth. If it looks clean you can then either transfer another piece to a new plate or several plates, or you can put a decent sized wedge into a grain jar.




I do both. :shrug:


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Re: Agar question [Re: Inocuole]
    #23461443 - 07/21/16 02:18 AM (7 years, 6 months ago)

I think the dude is confused about what specific spore to put on agar ... each spore is microscopic you will put alot of spores on agar it's not just one plus spores need to germinate with other spores so basically the spores have sex and produce the white mycelium ....


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Edited by Boogieman47 (07/21/16 02:21 AM)


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Re: Agar question [Re: Boogieman47] * 1
    #23461654 - 07/21/16 05:04 AM (7 years, 6 months ago)

I think that the first thing OP should do is look at some agar threads. You guys are trying to explain things which he has never even seen, let alone know what they would look like.

Here is something that can (maybe, but just maybe) help you out OP:

Step one, spore inoculation:
Basically, you do what pasty said, use any flame sterilized tool to scrape some spores from your print and put those on your agar (in a SAB, of course). If using spore syringe, just flame the needle and drop 1 or 2 drops:




Step two, waiting for the magic:

This is germination under a microscope, you can see mycelium started to grow from that single spore:


This is what germination will look like on a plate, you can see the mycelium growing under the surface of the agar:



Step three, transfers, clean growth, inoculation:

After spore germination pictured above, you need to do at least 2 transfers to ensure your culture is clean and also to allow enough time for mycelium to start showing you if it is organized or not. Organized mycelium has the best chance of being contam free, that is what you want.

This is organized and clean mycelium obtained after 2 transfers:


Once you get this organized mycelium, you can cut off wedges and drop them in your grain jars. You can use any sized wedge, the bigger the wedge/more wedges you throw in there, the faster colonization happens. I usually transfer wedges the size of 1/4 of the plate at a time, I will use 1 or 2 of these 1/4 plate wedges on a single jar.

This was more work than I thought it was going to be so I sure as shit hope you understand now OP. Regardless of if you think you understand or not, you still want to do more research. You can never learn enough in mycology.


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Offlinemaestro50
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Re: Agar question [Re: Supalemonhaze]
    #23461746 - 07/21/16 06:30 AM (7 years, 6 months ago)

Thanks guy a bunch! I really love this forum and appreciate the effort. I now understand what it is all about!

Ive also heard that the mycelium is faster then the bacteria... So the bacteria will evolve in the middle and the good mycelium will grow faster to the edges. So when im transfering the wedge i always took the most advanced one to the edge?

I have some syringes in my refrigerator for about two months so i will sure try it this week. Looking forward to it! The idea of obtaining perf. myc. this way seems logic and funn. One more thing... Where do i keep plates? cool and dark place?


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Re: Agar question [Re: maestro50]
    #23461901 - 07/21/16 07:53 AM (7 years, 6 months ago)

Put plates wherever, light is good for any stage of growth.

It depends on the bacteria. A bacteria infested plate can hinder mycelium growth, sometimes making it stall completely. Sometimes the mycelium is able to outrun it but that doesn't mean it's not carrying bacteria with it. Bacteria is by far the sneakiest type of contam. While the mycelium is growing, it can push bacteria with it allowing both organisms grow literally on top of each other.

If you ever get bacteria in a plate, make an agar sandwich to get rid of it. What you do is take your scalpel, flame it and dig a hole in a new agar plate. Then, take a transfer of bacteria contaminated mycelium and put that in the hole. Flame the scalpel again to kill off the bacteria that sticks to it and then cut a square piece of clean agar from your new plate and cover your transfer with it. What you will end up with is a contaminated transfer that is sandwiched between two pieces of agar. The mycelium will be able to grow through the agar while the bacteria gets left behind. This is much faster and safer than regular transfers when you want to get rid of bacteria, you can easily clean a culture up with just 1 sandwich.


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Re: Agar question [Re: Supalemonhaze]
    #23462633 - 07/21/16 01:11 PM (7 years, 6 months ago)

No I keep them on my table in the room lights are on 12 hours the house shouldn't be sweating your balls off hot but Ya that dark shit is way out dated use the search engine man there is everything you need there


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Offlinemaestro50
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Re: Agar question [Re: Boogieman47]
    #23463162 - 07/21/16 04:28 PM (7 years, 6 months ago)

Hello guys. Today i bought petri dish... i asked if it has the lid and she said yes.. then i get home and see this... its not sealed just one in another... can i somehow make lid for it or i can keep them closed like that... or buy something else? i payed 1.50$




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Re: Agar question [Re: maestro50]
    #23463199 - 07/21/16 04:38 PM (7 years, 6 months ago)

Look at this man https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 try and get those... those you have are one petri dish they don't seal you put the agar on the smaller plate and cover it with the bigger one but you will need to pressure cook those dude I really think you need to read more do you have a pressure cooker, a still air box or any of the crucial items we use for agar work? You can't skip corners on agar at all


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Edited by Boogieman47 (07/21/16 04:39 PM)


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Offlinemaestro50
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Re: Agar question [Re: Boogieman47]
    #23463291 - 07/21/16 05:16 PM (7 years, 6 months ago)

Quote:

noob47 said:
Look at this man https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 try and get those... those you have are one petri dish they don't seal you put the agar on the smaller plate and cover it with the bigger one but you will need to pressure cook those dude I really think you need to read more do you have a pressure cooker, a still air box or any of the crucial items we use for agar work? You can't skip corners on agar at all




Man im not noob... i have PC and SAB and all. Im just asking one question at a time about AGAR. i know all other things... So can i use it or not? Waiting other answers plz :mushroom2:


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Re: Agar question [Re: maestro50]
    #23463378 - 07/21/16 05:37 PM (7 years, 6 months ago)

Well I wasn't sure with some of the questions you're asking ... is it glass?? If so then you can wrap it in foil and PC it with your agar then you will have to take the agar out when it's still warm and pour it into the petri ddish and let it consolidate then drop one or two drops from your syringe on to the agar and wrap the dish with saran wrap and wait


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Re: Agar question [Re: Boogieman47]
    #23463578 - 07/21/16 06:33 PM (7 years, 6 months ago)

Ok thanks. Just dont know what do u mean by poruing it into the petri dish when its already in the petri dish. It is glass..

Also. should i somehow clean saran wrap?


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Re: Agar question [Re: maestro50]
    #23463643 - 07/21/16 06:57 PM (7 years, 6 months ago)

You can't PC the agar in the petri dish you will have to get a wine bottle or something and PC the agar in that wrap the petri dish in foil and PC the with the bottle for agar use the search engine for agar teks it will show you plenty plus those pasty plates I showed you are all reusable and you can PC the agar in those I think it's probably the easiest for a beginner


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Re: Agar question [Re: maestro50]
    #23463689 - 07/21/16 07:13 PM (7 years, 6 months ago)

Quote:

maestro50 said:


Man im not noob... Im just asking one question at a time about AGAR. i know all other things...




Quote:

maestro50 said:
Just dont know what do u mean by poruing it into the petri dish when its already in the petri dish.





:thatsinteresting:


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Re: Agar question [Re: Supalemonhaze]
    #23463707 - 07/21/16 07:19 PM (7 years, 6 months ago)

Well i dont know much about agar but learning. Why u take time to quote me but not help me lol. Im learning about pour and no pour right now..

I found this little 120 ml glass containers with metal lid... i think this will work.


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Re: Agar question [Re: Supalemonhaze]
    #23463708 - 07/21/16 07:19 PM (7 years, 6 months ago)

Haha


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Re: Agar question [Re: Boogieman47]
    #23463722 - 07/21/16 07:23 PM (7 years, 6 months ago)

Every one gets poked fun at man don't take it personal ... the best thing for you to do is read all the agar teks and people's threads on agar nobody can walk you through it any better then those


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Re: Agar question [Re: Boogieman47]
    #23463759 - 07/21/16 07:37 PM (7 years, 6 months ago)

Op you're not going to quit asking questions until you have the whole picture.
https://www.shroomery.org/forums/showflat.php/Number/23270429#23270429
Read and watch every single thread in that link, if you still don't get it after that it must mean you're mentally handicaped.


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Re: Agar question [Re: Josex]
    #23464161 - 07/21/16 09:53 PM (7 years, 6 months ago)

If you don't want to be laughed at, don't say "I'm not a noob" and then proceed to ask a bunch of noob questions.  Not having basic research skills does make you a noob, so the irony is hard to resist commenting on.

https://www.shroomery.org/forums/showflat.php/Number/22721954


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Re: Agar question [Re: Inocuole]
    #23464706 - 07/22/16 01:15 AM (7 years, 6 months ago)

Which is exactly why I decided to just spectate. :popcorn:


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Re: Agar question [Re: Supalemonhaze]
    #23464901 - 07/22/16 05:17 AM (7 years, 6 months ago)

Ok all. I will proceed on this journey by reading more and i guess trial and error. Its easy to get confused and lost in the sea of information but u shouldnt think that i dont read. I always read alot but still have some detailed questions that i dont know. Ill leave this topic so i can ask more questions if i have one later. thanks and cheers


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Re: Agar question [Re: maestro50]
    #23465043 - 07/22/16 07:02 AM (7 years, 6 months ago)

What you need to do is read good teks and start growing. That teaches loads. If you want to learn the finer points then lurk around on the boards and soak up them nuggets as they appear. If someone uses terms you don't understand, do a search on them. Learn what people to listen to and which to ignore. Do that for a year and you will be on good footing.


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Re: Agar question [Re: Pastywhyte]
    #23465104 - 07/22/16 07:28 AM (7 years, 6 months ago)

You don't have to do transfer to a second agar if using a biopsy from a fresh fruit right ? Couldn't anyone who's never done agar use a sealed 1/2 pint jar with rtf/Tyvek lid and shoot water in when the biopsy grows and they want to use it


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Edited by BaronVonBud (07/22/16 07:29 AM)


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Re: Agar question [Re: BaronVonBud]
    #23465107 - 07/22/16 07:29 AM (7 years, 6 months ago)

yes, you still want to do at least one transfer, even from a plate that was made from a tissue sample. you want to make sure it's clean, and if you made a plate with a clone sample, it will likely not be clean, since we dont fruit in sterile conditions.

and, yeah i guess you could do what youre suggesting, with shooting the water in a SHIP(i think that's what you are talking about?), or you could just make blenderless LI with a plate after you have ensured it' clean with transfers.

there are lots of ways to do things.


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Re: Agar question [Re: BaronVonBud]
    #23465112 - 07/22/16 07:32 AM (7 years, 6 months ago)

It's always a good idea to make a 2nd transfer when you first inoculate a plate. Clones commonly harbour bacteria. If the clone tissue is contaminated with mold spores, the mycelium will grow over them before they are able to germinate, making a second transfer will get rid of these mold spores whereas if you put them to grains, they will germinate and buttfuck your grain jar.


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Re: Agar question [Re: mupetmower]
    #23465115 - 07/22/16 07:35 AM (7 years, 6 months ago)

It's opening things up and cutting wedges with nothing but a still air box that has kept me from agar but I guess others do it


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Re: Agar question [Re: BaronVonBud]
    #23465122 - 07/22/16 07:39 AM (7 years, 6 months ago)

yeah man, SAB works wonders. just need good sterile technique.


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Re: Agar question [Re: mupetmower]
    #23465135 - 07/22/16 07:47 AM (7 years, 6 months ago)

What a terrible thing to let keep you from agar..


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Re: Agar question [Re: Inocuole]
    #23465431 - 07/22/16 09:42 AM (7 years, 6 months ago)

All of my agar work posted here is done in a SAB,


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Re: Agar question [Re: bodhisatta]
    #23466720 - 07/22/16 04:56 PM (7 years, 6 months ago)

I've never done G2G for the same reason. I don't have much issue with contaimination either and I know it's me and not the environment keeping it clean my house has never been really clean. I guess I need to take the leap

There's definitely a safety in using self healing ports and flamed needles that all noobs will make a crutch with


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Edited by BaronVonBud (07/22/16 04:58 PM)


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Re: Agar question [Re: BaronVonBud]
    #23466797 - 07/22/16 05:22 PM (7 years, 6 months ago)

I would say needles have a lower success rate if you use injection ports. With needles I like to still take the lid off in a SAB and inoculate right into the jar.

Then again I use ports out of simplicity but I understand I may have to toss more than the acceptable amount


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Re: Agar question [Re: bodhisatta] * 1
    #23467775 - 07/22/16 10:45 PM (7 years, 6 months ago)

Ports and needles are a pain in the ass and an unnecessary contam vector.


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Re: Agar question [Re: Inocuole]
    #23467794 - 07/22/16 10:49 PM (7 years, 6 months ago)

ISO on some RTV and a red hot needle going into a SAB seems and has been extremely clean. I guess wedges and G2G can also be how how can they be more clean ?


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Re: Agar question [Re: BaronVonBud]
    #23467800 - 07/22/16 10:51 PM (7 years, 6 months ago)

Put some iso on agar and watch the mold grow, then come back and explain how putting some on a RTV port is extremely clean.


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Re: Agar question [Re: BaronVonBud]
    #23467812 - 07/22/16 10:56 PM (7 years, 6 months ago)

Quote:

Trusted Cultivator said:
I would say needles have a lower success rate if you use injection ports. With needles I like to still take the lid off in a SAB and inoculate right into the jar.

Then again I use ports out of simplicity but I understand I may have to toss more than the acceptable amount




For me too. Ports are the reason why I couldn't keep an LC jar clean the last few times I tried it. For LCs, a plain hole covered with a sheet of tyvek seems to be better for me.

Quote:

BaronVonBud said:
ISO on some RTV and a red hot needle going into a SAB seems and has been extremely clean. I guess wedges and G2G can also be how how can they be more clean ?




Wedges is the one with the least contam vectors because it relies solely on your sterile tek. Leaky syringe, leaky ports and even small pieces of torn RTV/rubber can all easily contam your jars and they are out of your control when they do.

IME, RTV is the worst port you can make, proper rubber ports are more sturdy.


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Re: Agar question [Re: Supalemonhaze]
    #23467869 - 07/22/16 11:15 PM (7 years, 6 months ago)

I don't doubt that it's not extreme clean but it's transferring something through air into a jar exposed to air that gets me. I'll soon be expanding my horizons tho and learn


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Re: Agar question [Re: Supalemonhaze]
    #23467872 - 07/22/16 11:15 PM (7 years, 6 months ago)

The port can only be clean the first time you use it, assuming it was sterilized and had foil over it until you removed the foil in a SAB or in front of a flow hood.  If you ever need to draw anything from the port again after, it won't be clean because there is simply no easy way to re-sterilize the port without also fucking up everything else.

Air is just air, if the contaminants aren't floating in it, then it's the same as it traveling through a needle.  You have to trust that other people who've done this before know what they're talking about.  Everybody's a little apprehensive about SABs and sterile work.  When you're new using tools and needles and sterile vessels feels like the professional way to do things, you get to feeling real sciency having all that fancy gear, but it's really all about using your hands and taking advantage of still air, which means unlearning a few things about "Sterile technique" that you might've thought you knew beforehand.  And trust me, nobody is impressed with a still air box, I get it, they do not look sophisticated.  Simplicity is the way though.

I had to unlearn a lot of shit because I got into the hobby kinda stupidly thanks to this site not being the gem it is now, years ago, so you can take my word for it.


Edited by Inocuole (07/22/16 11:21 PM)


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Re: Agar question [Re: Inocuole]
    #23485557 - 07/28/16 12:08 PM (7 years, 6 months ago)

Ok guys. Back on my journey! :laugh:

I got these babies and i got agar and bit of honey.... for the potato... it has to be flakes or? In my store i have powder only... powder for making mash potato... Will it be good?

So for my containers... I make 5 holes and cover them with micro tape then inoculate the middle one?

My plan is to cook ingridients and pour them in little containers then PC all of it...

Am i on the right track?



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Re: Agar question [Re: maestro50]
    #23485559 - 07/28/16 12:08 PM (7 years, 6 months ago)

powder potatoes is just smaller flakes


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Re: Agar question [Re: bodhisatta]
    #23485610 - 07/28/16 12:24 PM (7 years, 6 months ago)

Ok thanks... Someone pls answer other questions because i need to do this today.


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Re: Agar question [Re: maestro50]
    #23485677 - 07/28/16 12:47 PM (7 years, 6 months ago)

why 5 holes where did you read that? follow the TEK are you doing the pastywhyte easy agaR?


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Re: Agar question [Re: maestro50]
    #23485679 - 07/28/16 12:47 PM (7 years, 6 months ago)

No you could just make one hole if it's just agar and cover that with micro pore tape but you inoculate after you PC cover the lids with foil once you PC let cool then take them out and put them into you sab or flow hood the go from there you only need a drop or two


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Re: Agar question [Re: Boogieman47]
    #23485718 - 07/28/16 12:58 PM (7 years, 6 months ago)

I saw it somewhere dont know where exactly guys. It was for FAE...?

Ok i will do one hole and inoculate after.

Noob47 u say put foil after PC... or before?


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Re: Agar question [Re: maestro50]
    #23485727 - 07/28/16 01:00 PM (7 years, 6 months ago)

stop guessing. follow directions

https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954

https://www.shroomery.org/forums/showflat.php/Number/19208976
(you can do this with glass jars and metal lids just do the same thing to the lid but drill a small hole rather than burn a small hole)


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Re: Agar question [Re: maestro50]
    #23485746 - 07/28/16 01:08 PM (7 years, 6 months ago)

Quote:

maestro50 said:
I saw it somewhere dont know where exactly guys. It was for FAE...?




You should know before even starting that FAE would ruin agar.


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Re: Agar question [Re: Inocuole]
    #23485802 - 07/28/16 01:25 PM (7 years, 6 months ago)

Thank u all! I will keep u updated once i see the white yummm


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Re: Agar question [Re: Boogieman47]
    #23485814 - 07/28/16 01:30 PM (7 years, 6 months ago)

Before, not to be rude I know how it is getting your toes wet in this hobby but everyone who is directing you to links or have gave you better than good info know their shit ... and if you keep not listening or asking questions that can easily be answered by reading the threads no ones going to want to help you when you might have some serious questions it's like the boy who cried wolf ... the jars you read about with 5 holes is for grain and you only need gas exchange for spawn jars the only reason we put holes in lids if for no pour so the pressure can escape and even in a pour tek it's good for releasing of condensation if I'm not wrong


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Re: Agar question [Re: Boogieman47]
    #23486132 - 07/28/16 03:14 PM (7 years, 6 months ago)

I understand and i do read the links. How do u think i have questions otherwise... Some tutorials are on point and very good and easy but some details are not mentioned everywhere. I can read whole 3 TEK's and still have some questions. I know its somewhat annoying to answer noob questions but it means alot... thank u all for keeping up with the noob heh


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Re: Agar question [Re: maestro50]
    #23498995 - 08/01/16 04:39 PM (7 years, 5 months ago)

Ok guys i know its early but eh! excited heh... does it look ok for now?



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Re: Agar question [Re: maestro50]
    #23499041 - 08/01/16 04:51 PM (7 years, 5 months ago)

you only need one or two drops less is more man it kinda looks OK but give it a day or two ... did you take the lid off in open air ? It will contam if you did and if you're only using foil as a lid you will always have shit get in there


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Re: Agar question [Re: Boogieman47]
    #23499077 - 08/01/16 05:06 PM (7 years, 5 months ago)

noo man i took photo in sab. and there is metal lid with micro tape too.

Can i keep other plates in fridge?

And i cant see inside when i inoculate so i just guessed... tryed to squeeze a bit. How can i do exactly one drop?


Edited by maestro50 (08/01/16 05:16 PM)


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Re: Agar question [Re: maestro50]
    #23499102 - 08/01/16 05:12 PM (7 years, 5 months ago)

Ya unused plates will be good in the fridge I'd keep the foil on till you're ready to use ...


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Re: Agar question [Re: Boogieman47]
    #23500633 - 08/02/16 03:26 AM (7 years, 5 months ago)

No need to put them in the fridge, if you get a contam in your plates, not having them in the fridge is actually helpful because they will grow faster. I would also remove the foil, trapped moisture between the foil and the lid can wick contams through the micropore tape. God knows it's not exactly the best filter around.

You should go ahead and take transfers from that. I take transfers from mine before the mycelium breaks the surface, that way you decrease the chances of allowing something else to grow with your culture.


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Re: Agar question [Re: Supalemonhaze]
    #23500737 - 08/02/16 05:47 AM (7 years, 5 months ago)

I made 4 od them... which one to take and transfer to?

And... is it necessary to PC them in order to clean jars?


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Re: Agar question [Re: maestro50]
    #23501472 - 08/02/16 11:08 AM (7 years, 5 months ago)

I'm not sure what you're asking.  You have to PC the jars with stuff inside of them, yes, if you want that stuff to be sterile.  Just.. extrapolate on that data point.


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Re: Agar question [Re: Inocuole]
    #23501544 - 08/02/16 11:29 AM (7 years, 5 months ago)

I was talking about when i reuse the jar... i just clean it with soap and then sterilize, and also sterilize when i will be making new agar mix or just wash and then reuse normally?

Also ive come to point where i dont know what to do with transfers. I have 4 inoculated jars and 2 jars with transfers and 4 more with agar in fridge... Do i now empty out the jars ive transfered from, because we will say the jar that i transferet to have cleaner colony...?


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Re: Agar question [Re: maestro50]
    #23501668 - 08/02/16 12:11 PM (7 years, 5 months ago)

Why would you sterilize it twice though?  Soap and water is fine to clean the grime off the jar.  Hell, you could skip that part too if you really wanted.  One sterilization per round is all it needs.  Whatever's stuck to the jar from the last round is going to get sterilized with the new batch anyway, and probably eaten by mycelium.  Good idea to keep it fairly clean though so stuff can't climb up and down the walls.

I'm not sure what kind of transfers you mean.  G2G?  A2G?  Why do you have inoculated jars in the fridge?  Are you the guy that wanted to inoculate jars, then put them in the fridge, then take them out when you want them to finish?  I recall having gone over that being a bad idea with someone recently.

Anyway, I'll assume you're talking about G2G donor jars, that you transfer from.  Those should be empty by the time you're done.  If they're not, you can just use them as spawn. 

If you're talking about agar plates you've transferred from already, either clean them out and reuse, or let them pin and take invitro clones.


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Re: Agar question [Re: Inocuole]
    #23501695 - 08/02/16 12:17 PM (7 years, 5 months ago)

No im keeping plain agar plates in fridge... for future use.

Man u have me so lost right now... Ok let me explain it how i see.

Ive inoculated few agar plates, and after i see good mycelium which means, its looking like tree roots or whatever... lots of strands in mycelium form, then i take a piece of that and transfer to another agar plate for it to develop more and when i see good mycelium formation then i can put that in grain...

Im so lost!!!!!!!!!!!! what am i doing lol pls


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Re: Agar question [Re: maestro50]
    #23501704 - 08/02/16 12:19 PM (7 years, 5 months ago)

It sounds like you're doing what you're supposed to be doing, which is why I'm confused.  All this and I still don't know exactly what your question is.  If the agar plates you're storing in the fridge are clean, then that's cool, I do the same.  If you have growth on agar that looks clean and it's been through a couple transfers, put it to grain.

I... am still lost on the question though.


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Re: Agar question [Re: Inocuole] * 1
    #23501842 - 08/02/16 01:04 PM (7 years, 5 months ago)

So once you get clean growth and put the clean wedge to grain you utter the magic incantation by the light of a blood moon. Only then will the mycelium leap off the wedge to colonize the grain. Say nothing to no one.


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Re: Agar question [Re: Pastywhyte]
    #23501865 - 08/02/16 01:11 PM (7 years, 5 months ago)

Quote:

Pastywhyte said:
So once you get clean growth and put the clean wedge to grain you utter the magic incantation by the light of a blood moon. Only then will the mycelium leap off the wedge to colonize the grain. Say nothing to no one.



If only I knew that sooner I wouldn't have been going through so much trouble thanks pasty!!!


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Re: Agar question [Re: Pastywhyte]
    #23501926 - 08/02/16 01:35 PM (7 years, 5 months ago)

haha pasty poetry :smile:

Ok im good now... on the track again. One more!!! lel

What do u get by transfering anyways. transfering same thing from same enviroment to same enviroment. I know there is something so im interested what is happening between transfers?


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Re: Agar question [Re: maestro50]
    #23501940 - 08/02/16 01:40 PM (7 years, 5 months ago)



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Re: Agar question [Re: Inocuole]
    #23504442 - 08/03/16 04:38 AM (7 years, 5 months ago)

This is first time seeing this.

Thank u all very much <3


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Re: Agar question [Re: maestro50]
    #23543235 - 08/15/16 08:59 AM (7 years, 5 months ago)

Ok guys so after some time and transfers i have 6 more i made today... This is the one of the dishes i transfered... How does it look and what do u think about places ive took transfers? The pic is a bit bad but i took it from toilet after remembering to take picture heh



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Re: Agar question [Re: maestro50]
    #23546002 - 08/16/16 04:58 AM (7 years, 5 months ago)

guyssss


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Re: Agar question [Re: maestro50]
    #23546016 - 08/16/16 05:09 AM (7 years, 5 months ago)

Looks good.

Can't tell what the sections you took transfers from looked like because you removed them.

I would have taken them from the upper left corner, 10 o'clock.


Edited by jshrnld (08/16/16 05:11 AM)


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Re: Agar question [Re: maestro50]
    #23546299 - 08/16/16 08:39 AM (7 years, 5 months ago)

Quote:

maestro50 said:
Ok guys so after some time and transfers i have 6 more i made today... This is the one of the dishes i transfered... How does it look and what do u think about places ive took transfers? The pic is a bit bad but i took it from toilet after remembering to take picture heh






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Re: Agar question [Re: wtfcrazymofo]
    #23559132 - 08/20/16 04:32 AM (7 years, 5 months ago)

Ok guys i think i have pretty uniformed growth now... the outer perimeter is looking nice and stable... presuming i have a good culture now. How do i go to keeping my mycelium for future. storing it?

And i have 4 good looking agar plates now. Must i grow them all and see performance or i can take one that looks most badass?


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Re: Agar question [Re: maestro50]
    #23562035 - 08/21/16 02:45 AM (7 years, 5 months ago)

anyone?? my plates are full.


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Re: Agar question [Re: maestro50]
    #23562039 - 08/21/16 02:48 AM (7 years, 5 months ago)

I'm not sure what "looking badass" entails, but if you could determine performance and potency just by looking at a culture nobody would bother testing anything.  So, either do everything, or grow what looks best, but the more options the better.


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Re: Agar question [Re: Inocuole]
    #23562271 - 08/21/16 07:23 AM (7 years, 5 months ago)

Well u can determine by looking at it right? monoculture have same edges from centre... and are rhizomorphic

Also if someone could say how to keep good agar for future... pls help me now at my agar endings... The myc is growing and i dont know what do to next


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Re: Agar question [Re: maestro50]
    #23562614 - 08/21/16 10:40 AM (7 years, 5 months ago)

Monoculture could be tomentose too. Rhizomorphic doesn't really mean anything


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Re: Agar question [Re: bodhisatta]
    #23562723 - 08/21/16 11:18 AM (7 years, 5 months ago)

Then how do i determine the quality?
Ive really searched on agar myc storing and didnt find much so once again if u could help me. 5 Pages of writing and now nobody wants to help me with storing the actual product


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Re: Agar question [Re: maestro50]
    #23562727 - 08/21/16 11:20 AM (7 years, 5 months ago)

You have to test it by growing it out.

Storage is in a refrigerator on the dish or on a slant.


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Re: Agar question [Re: bodhisatta]
    #23562974 - 08/21/16 12:58 PM (7 years, 5 months ago)

Ok. Just today i figured it out that i can test multiple plates in one grow by transfering each one to grain and labeling... ahh me :laugh:

For the storage... i just put plate in refrigerator and it stops growing, and it is preserved?

btw how do i test it corretly... i imagine i eat half of g then wait a copuple days then eat another jar?


Edited by maestro50 (08/21/16 01:12 PM)


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Re: Agar question [Re: maestro50]
    #23563059 - 08/21/16 01:35 PM (7 years, 5 months ago)

It doesn't stop growing in the fridge. Look it up


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Re: Agar question [Re: bodhisatta]
    #23563074 - 08/21/16 01:43 PM (7 years, 5 months ago)

ive really searched but nothing. So can someone finally say how do i store mycelium??

I dont know why its so hard for u to help me now that im at the end. in the beginning all of u were so supportive and now nothing. i have simple question. i will transfer one piece to another plate and grow this one but how do i preserve the transfer?

is that it?

https://www.shroomery.org/8511/Practical-method-for-long-term-storage-of-mycellium-in-dH2O-using-ependorf-tubes-as-vials


Edited by maestro50 (08/21/16 02:04 PM)


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Re: Agar question [Re: maestro50]
    #23563182 - 08/21/16 02:50 PM (7 years, 5 months ago)



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Re: Agar question [Re: bodhisatta] * 1
    #23563351 - 08/21/16 04:12 PM (7 years, 5 months ago)

Links the link that has popped up on this page in his sig like 5 times.. after dude says he has searched. :lol:

Love a good dose of irony.


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Re: Agar question [Re: Inocuole]
    #23565184 - 08/22/16 07:09 AM (7 years, 5 months ago)

Guys isnt the forum for noobs? I believe everything is written on the internet then why have forum?

Some things are obviously tough and complicated for new agar users and dont be so harsh. Now i have feeling i cant ask many questions and therefore i may do something wrong... And u cant say its hard for u to help someone and hit me up with link or a few words... I always help people when i can, u are literally helping expanding world consciousness and peace but no its too hard for u.


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Re: Agar question [Re: maestro50]
    #23565191 - 08/22/16 07:17 AM (7 years, 5 months ago)

woa, I never knew we had a write up on distilled H20,and in the FAQ section at that :lol:

this is the internet, don't get upset. its not worth it.  stareatclouds used to ask a million questions, many thought it was annoying.  now if u look at his recent grows, they are pimped out.

u go at ur own pace and style.  people answer with their own pace and style.  yea, people will clash.  and so it is in real life too.  its all good tho mang:cool::mushroom2:


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Re: Agar question [Re: maestro50]
    #23565466 - 08/22/16 09:42 AM (7 years, 5 months ago)

Quote:

maestro50 said:
Guys isnt the forum for noobs? I believe everything is written on the internet then why have forum?

Some things are obviously tough and complicated for new agar users and dont be so harsh. Now i have feeling i cant ask many questions and therefore i may do something wrong... And u cant say its hard for u to help someone and hit me up with link or a few words... I always help people when i can, u are literally helping expanding world consciousness and peace but no its too hard for u.



:facepalm:
All of us spend all this time writing down what we know for free. If trying to get you the most mushrooms for your effort isn't shroomy and being nice then Idk man...

Sometimes it takes some of your efforts too. There's a dickload of noobs that can do a lot more with the info they're given


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Re: Agar question [Re: bodhisatta]
    #23565476 - 08/22/16 09:47 AM (7 years, 5 months ago)

Yes but i am pedant as possible... perfectionist when i do something like this so i want to know all the details... I surely apprecitate all the effort for FREE! lol

Ok so i gotta get supplies and my plates are grown up so i made another transfer before storing transfers into dH20 and graining new plates for test... here is one picture... they look pretty mono to me... except that one. Cheers <3



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Re: Agar question [Re: maestro50]
    #23565852 - 08/22/16 12:22 PM (7 years, 5 months ago)

In dH2O tek it doesnt say anything about PCing the stuff.. so u tell me should i ask u do i need to PC my stuff?? well thats pretty important. But it doesnt say anything. awesome!!


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Re: Agar question [Re: maestro50]
    #23565868 - 08/22/16 12:27 PM (7 years, 5 months ago)

Which tek?


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Re: Agar question [Re: Inocuole]
    #23565916 - 08/22/16 12:43 PM (7 years, 5 months ago)

:whoak:


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Re: Agar question [Re: maestro50]
    #23566294 - 08/22/16 03:16 PM (7 years, 5 months ago)

Quote:

maestro50 said:
Yes but i am pedant as possible... perfectionist when i do something like this so i want to know all the details... I surely apprecitate all the effort for FREE! lol

Ok so i gotta get supplies and my plates are grown up so i made another transfer before storing transfers into dH20 and graining new plates for test... here is one picture... they look pretty mono to me... except that one. Cheers <3






So I just read this whole thread and all I can say is wow man, just wow. 

You are seriously fucking with these guys.  You act like you are in a sketch comedy or something but you seem to know what you're doing by the pics. 

And then you bring up storing cultures in distilled water, which you'd only know about if you did a little research already.  I assume you already know if you store a culture in pure distilled water they will go into a dormant state.  This is true for a lot of microorganisms. 

But then you act like you didn't know you still have to p.c.  it.  You know, you really do.  You've came this far already which you couldn't have done without decent sterile tek.  Maybe you should put all your work in a journal or something. 

It's still a pretty amusing thread though


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Re: Agar question [Re: Kenetic]
    #23566325 - 08/22/16 03:28 PM (7 years, 5 months ago)

Im doing this tek

https://www.shroomery.org/8511/Practical-method-for-long-term-storage-of-mycellium-in-dH2O-using-ependorf-tubes-as-vials

Well as u see i am perfectionist and i achieved all that i wanted. How? maybe i fucked too much with these guys... as u said. this is my first agar and no signs of contam at all... because i love to know every detail right. And be a bit annoying. I do many things in my life and learn alot so when the question pops up about something a research a bit but when i dont find it or seem unclear i open a thread or ask a question. Im sure alot of noobs and other guys may learn something from my thread.

And i would love to make journal! I have my ways of doing thing even better but first i must know basics. Guys i appreciate help really do and i dont mind being noobish and annoying. Its better for me to ask then trial and error.

I have one more question plssssssss

So today i bought ependorf. Do i pc them, pipette, and cotton swabs? Ok again i feel like idiot asking this but i dont like assuming..

TNXXXXXXXXXX


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Re: Agar question [Re: maestro50]
    #23566408 - 08/22/16 03:54 PM (7 years, 5 months ago)

Last link in my signature I would pick one of the storage methods located there.

Slants ala RR or frank


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Re: Agar question [Re: bodhisatta]
    #23568690 - 08/23/16 10:14 AM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Last link in my signature I would pick one of the storage methods located there.

Slants ala RR or frank




ive already bought ependorfins... dh20 tek isnt good? I mean its on the main agar page on forum


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Re: Agar question [Re: maestro50]
    #23568949 - 08/23/16 11:54 AM (7 years, 5 months ago)

Quote:

maestro50 said:
ive already bought ependorfins...




What?

Quote:

maestro50 said:
dh20 tek isnt good? I mean its on the main agar page on forum



Where..?  Which agar page on the forum?  Only one I can find is this old ass thing full of ancient techniques that nobody uses anymore.

https://www.shroomery.org/forums/postlist.php/Board/59


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Re: Agar question [Re: Inocuole]
    #23570524 - 08/23/16 08:35 PM (7 years, 5 months ago)



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Re: Agar question [Re: maestro50]
    #23571029 - 08/23/16 10:28 PM (7 years, 5 months ago)

I see, that's  on the main site, not the forums, which explains why I couldn't find it.  It's also from 2004.  Other than that I don't see anything particularly wrong with it, but I also don't see anything wrong with storing your cultures on slants like most people.


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Re: Agar question [Re: Inocuole]
    #23571048 - 08/23/16 10:32 PM (7 years, 5 months ago)

I don't like that it suggests 50% survival rate. That's pretty damn low given the difficulty in executing that process cleanly. I have had a few cultures die in slants but those were all without wood and kept for a long time. A proper master slant with wood will be good for a long time. I just revived one that was going on 3 years in the fridge.


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Re: Agar question [Re: Pastywhyte]
    #23571814 - 08/24/16 04:29 AM (7 years, 5 months ago)

my buddy used dh20 for chicken of the woods cause they eat up agars fast. 

I don't think u need to get all the air bubbles out of the mini tubes like it says, but Ill ask him what he does.  if u do, then thats annoying…maybe not worth it considering how small those are to work with.

Quote:

A proper master slant with wood will be good for a long time




amen


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Re: Agar question [Re: blindingleaf]
    #23571966 - 08/24/16 06:24 AM (7 years, 5 months ago)

Can i somehow use this tubes with wood maybe... because i already got them and its hard for me to find tubes from the other tek... But if its no good ill do the other one. I just like simplicity and size of this tubes


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Re: Agar question [Re: maestro50]
    #23571974 - 08/24/16 06:28 AM (7 years, 5 months ago)

it would be too small to treat them like a proper slant.

distilled is a legitimate way to store cultures, its just not as common on the boards, so its  not generally recommended. 

I don't know where that 50% failure rate number came from in that guys write up, seems kinda high.


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Re: Agar question [Re: blindingleaf]
    #23571993 - 08/24/16 06:36 AM (7 years, 5 months ago)

the way my buddy did it is just drop wedges in centrifuge tubes of distilled water.  he didn't do all that swabbing and pipette shit.  Im just not sure if he made it so there were no air bubbles. I doubt he did that considering he isn't the tightest with technique.


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Re: Agar question [Re: blindingleaf]
    #23573384 - 08/24/16 04:07 PM (7 years, 5 months ago)

I will do dH2O and see how it goes... ill be clean so thumbs up. I just think the other tek is too much. U just need to preserve the tiny thing sterile.


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Re: Agar question [Re: maestro50]
    #23573388 - 08/24/16 04:08 PM (7 years, 5 months ago)

Tonight I'm making slants for the first time. I don't have parafilm as I've never used it....would micropore tape be a fine substitute for taping around the caps?



Once I PC these I'll put them on the bench at a slight angle and drop clean wedges in once they solidify. I wish they had a wider mouthed, its going to be a pain getting wedges out when I need them again,  but I'm working with what I've got.

I plan on keeping them stored in a ziplock inside of jar with SFD filter inside the fridge.

Tips?


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