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InvisibleInocuole
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Re: Agar question [Re: maestro50]
    #23565868 - 08/22/16 12:27 PM (7 years, 5 months ago)

Which tek?


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Invisiblemorty422
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Re: Agar question [Re: Inocuole]
    #23565916 - 08/22/16 12:43 PM (7 years, 5 months ago)

:whoak:


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OfflineKenetic
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Re: Agar question [Re: maestro50]
    #23566294 - 08/22/16 03:16 PM (7 years, 5 months ago)

Quote:

maestro50 said:
Yes but i am pedant as possible... perfectionist when i do something like this so i want to know all the details... I surely apprecitate all the effort for FREE! lol

Ok so i gotta get supplies and my plates are grown up so i made another transfer before storing transfers into dH20 and graining new plates for test... here is one picture... they look pretty mono to me... except that one. Cheers <3






So I just read this whole thread and all I can say is wow man, just wow. 

You are seriously fucking with these guys.  You act like you are in a sketch comedy or something but you seem to know what you're doing by the pics. 

And then you bring up storing cultures in distilled water, which you'd only know about if you did a little research already.  I assume you already know if you store a culture in pure distilled water they will go into a dormant state.  This is true for a lot of microorganisms. 

But then you act like you didn't know you still have to p.c.  it.  You know, you really do.  You've came this far already which you couldn't have done without decent sterile tek.  Maybe you should put all your work in a journal or something. 

It's still a pretty amusing thread though


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Offlinemaestro50
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Re: Agar question [Re: Kenetic]
    #23566325 - 08/22/16 03:28 PM (7 years, 5 months ago)

Im doing this tek

https://www.shroomery.org/8511/Practical-method-for-long-term-storage-of-mycellium-in-dH2O-using-ependorf-tubes-as-vials

Well as u see i am perfectionist and i achieved all that i wanted. How? maybe i fucked too much with these guys... as u said. this is my first agar and no signs of contam at all... because i love to know every detail right. And be a bit annoying. I do many things in my life and learn alot so when the question pops up about something a research a bit but when i dont find it or seem unclear i open a thread or ask a question. Im sure alot of noobs and other guys may learn something from my thread.

And i would love to make journal! I have my ways of doing thing even better but first i must know basics. Guys i appreciate help really do and i dont mind being noobish and annoying. Its better for me to ask then trial and error.

I have one more question plssssssss

So today i bought ependorf. Do i pc them, pipette, and cotton swabs? Ok again i feel like idiot asking this but i dont like assuming..

TNXXXXXXXXXX


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InvisiblebodhisattaMDiscordReddit
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Re: Agar question [Re: maestro50]
    #23566408 - 08/22/16 03:54 PM (7 years, 5 months ago)

Last link in my signature I would pick one of the storage methods located there.

Slants ala RR or frank


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Offlinemaestro50
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Re: Agar question [Re: bodhisatta]
    #23568690 - 08/23/16 10:14 AM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Last link in my signature I would pick one of the storage methods located there.

Slants ala RR or frank




ive already bought ependorfins... dh20 tek isnt good? I mean its on the main agar page on forum


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InvisibleInocuole
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Re: Agar question [Re: maestro50]
    #23568949 - 08/23/16 11:54 AM (7 years, 5 months ago)

Quote:

maestro50 said:
ive already bought ependorfins...




What?

Quote:

maestro50 said:
dh20 tek isnt good? I mean its on the main agar page on forum



Where..?  Which agar page on the forum?  Only one I can find is this old ass thing full of ancient techniques that nobody uses anymore.

https://www.shroomery.org/forums/postlist.php/Board/59


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Offlinemaestro50
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Re: Agar question [Re: Inocuole]
    #23570524 - 08/23/16 08:35 PM (7 years, 5 months ago)



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InvisibleInocuole
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Re: Agar question [Re: maestro50]
    #23571029 - 08/23/16 10:28 PM (7 years, 5 months ago)

I see, that's  on the main site, not the forums, which explains why I couldn't find it.  It's also from 2004.  Other than that I don't see anything particularly wrong with it, but I also don't see anything wrong with storing your cultures on slants like most people.


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InvisiblePastywhyteMDiscord
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Re: Agar question [Re: Inocuole]
    #23571048 - 08/23/16 10:32 PM (7 years, 5 months ago)

I don't like that it suggests 50% survival rate. That's pretty damn low given the difficulty in executing that process cleanly. I have had a few cultures die in slants but those were all without wood and kept for a long time. A proper master slant with wood will be good for a long time. I just revived one that was going on 3 years in the fridge.


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Invisibleblindingleaf
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Re: Agar question [Re: Pastywhyte]
    #23571814 - 08/24/16 04:29 AM (7 years, 5 months ago)

my buddy used dh20 for chicken of the woods cause they eat up agars fast. 

I don't think u need to get all the air bubbles out of the mini tubes like it says, but Ill ask him what he does.  if u do, then thats annoying…maybe not worth it considering how small those are to work with.

Quote:

A proper master slant with wood will be good for a long time




amen


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Offlinemaestro50
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Re: Agar question [Re: blindingleaf]
    #23571966 - 08/24/16 06:24 AM (7 years, 5 months ago)

Can i somehow use this tubes with wood maybe... because i already got them and its hard for me to find tubes from the other tek... But if its no good ill do the other one. I just like simplicity and size of this tubes


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Invisibleblindingleaf
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Re: Agar question [Re: maestro50]
    #23571974 - 08/24/16 06:28 AM (7 years, 5 months ago)

it would be too small to treat them like a proper slant.

distilled is a legitimate way to store cultures, its just not as common on the boards, so its  not generally recommended. 

I don't know where that 50% failure rate number came from in that guys write up, seems kinda high.


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Invisibleblindingleaf
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Re: Agar question [Re: blindingleaf]
    #23571993 - 08/24/16 06:36 AM (7 years, 5 months ago)

the way my buddy did it is just drop wedges in centrifuge tubes of distilled water.  he didn't do all that swabbing and pipette shit.  Im just not sure if he made it so there were no air bubbles. I doubt he did that considering he isn't the tightest with technique.


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Offlinemaestro50
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Re: Agar question [Re: blindingleaf]
    #23573384 - 08/24/16 04:07 PM (7 years, 5 months ago)

I will do dH2O and see how it goes... ill be clean so thumbs up. I just think the other tek is too much. U just need to preserve the tiny thing sterile.


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OfflinePsilocyBen17
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Re: Agar question [Re: maestro50]
    #23573388 - 08/24/16 04:08 PM (7 years, 5 months ago)

Tonight I'm making slants for the first time. I don't have parafilm as I've never used it....would micropore tape be a fine substitute for taping around the caps?



Once I PC these I'll put them on the bench at a slight angle and drop clean wedges in once they solidify. I wish they had a wider mouthed, its going to be a pain getting wedges out when I need them again,  but I'm working with what I've got.

I plan on keeping them stored in a ziplock inside of jar with SFD filter inside the fridge.

Tips?


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