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maestro50
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Re: Agar question [Re: Inocuole]
#23485802 - 07/28/16 01:25 PM (7 years, 6 months ago) |
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Thank u all! I will keep u updated once i see the white yummm
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Before, not to be rude I know how it is getting your toes wet in this hobby but everyone who is directing you to links or have gave you better than good info know their shit ... and if you keep not listening or asking questions that can easily be answered by reading the threads no ones going to want to help you when you might have some serious questions it's like the boy who cried wolf ... the jars you read about with 5 holes is for grain and you only need gas exchange for spawn jars the only reason we put holes in lids if for no pour so the pressure can escape and even in a pour tek it's good for releasing of condensation if I'm not wrong
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maestro50
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I understand and i do read the links. How do u think i have questions otherwise... Some tutorials are on point and very good and easy but some details are not mentioned everywhere. I can read whole 3 TEK's and still have some questions. I know its somewhat annoying to answer noob questions but it means alot... thank u all for keeping up with the noob heh
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maestro50
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Ok guys i know its early but eh! excited heh... does it look ok for now?
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Boogieman47
Let's boogie


Registered: 03/05/16
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you only need one or two drops less is more man it kinda looks OK but give it a day or two ... did you take the lid off in open air ? It will contam if you did and if you're only using foil as a lid you will always have shit get in there
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maestro50
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noo man i took photo in sab. and there is metal lid with micro tape too.
Can i keep other plates in fridge?
And i cant see inside when i inoculate so i just guessed... tryed to squeeze a bit. How can i do exactly one drop?
Edited by maestro50 (08/01/16 05:16 PM)
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Boogieman47
Let's boogie


Registered: 03/05/16
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Loc: Under your bed
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Ya unused plates will be good in the fridge I'd keep the foil on till you're ready to use ...
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Loc: 12" down Europe's butthole
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No need to put them in the fridge, if you get a contam in your plates, not having them in the fridge is actually helpful because they will grow faster. I would also remove the foil, trapped moisture between the foil and the lid can wick contams through the micropore tape. God knows it's not exactly the best filter around.
You should go ahead and take transfers from that. I take transfers from mine before the mycelium breaks the surface, that way you decrease the chances of allowing something else to grow with your culture.
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maestro50
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I made 4 od them... which one to take and transfer to?
And... is it necessary to PC them in order to clean jars?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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I'm not sure what you're asking. You have to PC the jars with stuff inside of them, yes, if you want that stuff to be sterile. Just.. extrapolate on that data point.
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maestro50
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Re: Agar question [Re: Inocuole]
#23501544 - 08/02/16 11:29 AM (7 years, 5 months ago) |
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I was talking about when i reuse the jar... i just clean it with soap and then sterilize, and also sterilize when i will be making new agar mix or just wash and then reuse normally?
Also ive come to point where i dont know what to do with transfers. I have 4 inoculated jars and 2 jars with transfers and 4 more with agar in fridge... Do i now empty out the jars ive transfered from, because we will say the jar that i transferet to have cleaner colony...?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Why would you sterilize it twice though? Soap and water is fine to clean the grime off the jar. Hell, you could skip that part too if you really wanted. One sterilization per round is all it needs. Whatever's stuck to the jar from the last round is going to get sterilized with the new batch anyway, and probably eaten by mycelium. Good idea to keep it fairly clean though so stuff can't climb up and down the walls.
I'm not sure what kind of transfers you mean. G2G? A2G? Why do you have inoculated jars in the fridge? Are you the guy that wanted to inoculate jars, then put them in the fridge, then take them out when you want them to finish? I recall having gone over that being a bad idea with someone recently.
Anyway, I'll assume you're talking about G2G donor jars, that you transfer from. Those should be empty by the time you're done. If they're not, you can just use them as spawn.
If you're talking about agar plates you've transferred from already, either clean them out and reuse, or let them pin and take invitro clones.
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maestro50
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Re: Agar question [Re: Inocuole]
#23501695 - 08/02/16 12:17 PM (7 years, 5 months ago) |
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No im keeping plain agar plates in fridge... for future use.
Man u have me so lost right now... Ok let me explain it how i see.
Ive inoculated few agar plates, and after i see good mycelium which means, its looking like tree roots or whatever... lots of strands in mycelium form, then i take a piece of that and transfer to another agar plate for it to develop more and when i see good mycelium formation then i can put that in grain...
Im so lost!!!!!!!!!!!! what am i doing lol pls
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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It sounds like you're doing what you're supposed to be doing, which is why I'm confused. All this and I still don't know exactly what your question is. If the agar plates you're storing in the fridge are clean, then that's cool, I do the same. If you have growth on agar that looks clean and it's been through a couple transfers, put it to grain.
I... am still lost on the question though.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Agar question [Re: Inocuole] 1
#23501842 - 08/02/16 01:04 PM (7 years, 5 months ago) |
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So once you get clean growth and put the clean wedge to grain you utter the magic incantation by the light of a blood moon. Only then will the mycelium leap off the wedge to colonize the grain. Say nothing to no one.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Quote:
Pastywhyte said: So once you get clean growth and put the clean wedge to grain you utter the magic incantation by the light of a blood moon. Only then will the mycelium leap off the wedge to colonize the grain. Say nothing to no one.
If only I knew that sooner I wouldn't have been going through so much trouble thanks pasty!!!
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maestro50
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haha pasty poetry 
Ok im good now... on the track again. One more!!! lel
What do u get by transfering anyways. transfering same thing from same enviroment to same enviroment. I know there is something so im interested what is happening between transfers?
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Inocuole
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maestro50
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Re: Agar question [Re: Inocuole]
#23504442 - 08/03/16 04:38 AM (7 years, 5 months ago) |
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This is first time seeing this.
Thank u all very much <3
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maestro50
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Ok guys so after some time and transfers i have 6 more i made today... This is the one of the dishes i transfered... How does it look and what do u think about places ive took transfers? The pic is a bit bad but i took it from toilet after remembering to take picture heh
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