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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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It's opening things up and cutting wedges with nothing but a still air box that has kept me from agar but I guess others do it
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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yeah man, SAB works wonders. just need good sterile technique.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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What a terrible thing to let keep you from agar..
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Agar question [Re: Inocuole]
#23465431 - 07/22/16 09:42 AM (7 years, 6 months ago) |
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All of my agar work posted here is done in a SAB,
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BaronVonBud
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Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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I've never done G2G for the same reason. I don't have much issue with contaimination either and I know it's me and not the environment keeping it clean my house has never been really clean. I guess I need to take the leap
There's definitely a safety in using self healing ports and flamed needles that all noobs will make a crutch with
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Edited by BaronVonBud (07/22/16 04:58 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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I would say needles have a lower success rate if you use injection ports. With needles I like to still take the lid off in a SAB and inoculate right into the jar.
Then again I use ports out of simplicity but I understand I may have to toss more than the acceptable amount
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Ports and needles are a pain in the ass and an unnecessary contam vector.
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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Re: Agar question [Re: Inocuole]
#23467794 - 07/22/16 10:49 PM (7 years, 6 months ago) |
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ISO on some RTV and a red hot needle going into a SAB seems and has been extremely clean. I guess wedges and G2G can also be how how can they be more clean ?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Put some iso on agar and watch the mold grow, then come back and explain how putting some on a RTV port is extremely clean.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
Trusted Cultivator said: I would say needles have a lower success rate if you use injection ports. With needles I like to still take the lid off in a SAB and inoculate right into the jar.
Then again I use ports out of simplicity but I understand I may have to toss more than the acceptable amount
For me too. Ports are the reason why I couldn't keep an LC jar clean the last few times I tried it. For LCs, a plain hole covered with a sheet of tyvek seems to be better for me.
Quote:
BaronVonBud said: ISO on some RTV and a red hot needle going into a SAB seems and has been extremely clean. I guess wedges and G2G can also be how how can they be more clean ?
Wedges is the one with the least contam vectors because it relies solely on your sterile tek. Leaky syringe, leaky ports and even small pieces of torn RTV/rubber can all easily contam your jars and they are out of your control when they do.
IME, RTV is the worst port you can make, proper rubber ports are more sturdy.
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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I don't doubt that it's not extreme clean but it's transferring something through air into a jar exposed to air that gets me. I'll soon be expanding my horizons tho and learn
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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The port can only be clean the first time you use it, assuming it was sterilized and had foil over it until you removed the foil in a SAB or in front of a flow hood. If you ever need to draw anything from the port again after, it won't be clean because there is simply no easy way to re-sterilize the port without also fucking up everything else.
Air is just air, if the contaminants aren't floating in it, then it's the same as it traveling through a needle. You have to trust that other people who've done this before know what they're talking about. Everybody's a little apprehensive about SABs and sterile work. When you're new using tools and needles and sterile vessels feels like the professional way to do things, you get to feeling real sciency having all that fancy gear, but it's really all about using your hands and taking advantage of still air, which means unlearning a few things about "Sterile technique" that you might've thought you knew beforehand. And trust me, nobody is impressed with a still air box, I get it, they do not look sophisticated. Simplicity is the way though.
I had to unlearn a lot of shit because I got into the hobby kinda stupidly thanks to this site not being the gem it is now, years ago, so you can take my word for it.
Edited by Inocuole (07/22/16 11:21 PM)
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maestro50
Stranger
Registered: 12/08/15
Posts: 199
Last seen: 3 years, 4 months
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Re: Agar question [Re: Inocuole]
#23485557 - 07/28/16 12:08 PM (7 years, 6 months ago) |
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Ok guys. Back on my journey! 
I got these babies and i got agar and bit of honey.... for the potato... it has to be flakes or? In my store i have powder only... powder for making mash potato... Will it be good?
So for my containers... I make 5 holes and cover them with micro tape then inoculate the middle one?
My plan is to cook ingridients and pour them in little containers then PC all of it...
Am i on the right track?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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powder potatoes is just smaller flakes
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maestro50
Stranger
Registered: 12/08/15
Posts: 199
Last seen: 3 years, 4 months
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Ok thanks... Someone pls answer other questions because i need to do this today.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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why 5 holes where did you read that? follow the TEK are you doing the pastywhyte easy agaR?
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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No you could just make one hole if it's just agar and cover that with micro pore tape but you inoculate after you PC cover the lids with foil once you PC let cool then take them out and put them into you sab or flow hood the go from there you only need a drop or two
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maestro50
Stranger
Registered: 12/08/15
Posts: 199
Last seen: 3 years, 4 months
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I saw it somewhere dont know where exactly guys. It was for FAE...?
Ok i will do one hole and inoculate after.
Noob47 u say put foil after PC... or before?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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stop guessing. follow directions
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954
https://www.shroomery.org/forums/showflat.php/Number/19208976 (you can do this with glass jars and metal lids just do the same thing to the lid but drill a small hole rather than burn a small hole)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
maestro50 said: I saw it somewhere dont know where exactly guys. It was for FAE...?
You should know before even starting that FAE would ruin agar.
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