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InvisibleSupalemonhaze
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Re: Agar question [Re: maestro50]
    #23461901 - 07/21/16 07:53 AM (7 years, 6 months ago)

Put plates wherever, light is good for any stage of growth.

It depends on the bacteria. A bacteria infested plate can hinder mycelium growth, sometimes making it stall completely. Sometimes the mycelium is able to outrun it but that doesn't mean it's not carrying bacteria with it. Bacteria is by far the sneakiest type of contam. While the mycelium is growing, it can push bacteria with it allowing both organisms grow literally on top of each other.

If you ever get bacteria in a plate, make an agar sandwich to get rid of it. What you do is take your scalpel, flame it and dig a hole in a new agar plate. Then, take a transfer of bacteria contaminated mycelium and put that in the hole. Flame the scalpel again to kill off the bacteria that sticks to it and then cut a square piece of clean agar from your new plate and cover your transfer with it. What you will end up with is a contaminated transfer that is sandwiched between two pieces of agar. The mycelium will be able to grow through the agar while the bacteria gets left behind. This is much faster and safer than regular transfers when you want to get rid of bacteria, you can easily clean a culture up with just 1 sandwich.


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InvisibleBoogieman47
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Re: Agar question [Re: Supalemonhaze]
    #23462633 - 07/21/16 01:11 PM (7 years, 6 months ago)

No I keep them on my table in the room lights are on 12 hours the house shouldn't be sweating your balls off hot but Ya that dark shit is way out dated use the search engine man there is everything you need there


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Offlinemaestro50
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Re: Agar question [Re: Boogieman47]
    #23463162 - 07/21/16 04:28 PM (7 years, 6 months ago)

Hello guys. Today i bought petri dish... i asked if it has the lid and she said yes.. then i get home and see this... its not sealed just one in another... can i somehow make lid for it or i can keep them closed like that... or buy something else? i payed 1.50$




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InvisibleBoogieman47
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Re: Agar question [Re: maestro50]
    #23463199 - 07/21/16 04:38 PM (7 years, 6 months ago)

Look at this man https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 try and get those... those you have are one petri dish they don't seal you put the agar on the smaller plate and cover it with the bigger one but you will need to pressure cook those dude I really think you need to read more do you have a pressure cooker, a still air box or any of the crucial items we use for agar work? You can't skip corners on agar at all


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Edited by Boogieman47 (07/21/16 04:39 PM)


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Offlinemaestro50
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Re: Agar question [Re: Boogieman47]
    #23463291 - 07/21/16 05:16 PM (7 years, 6 months ago)

Quote:

noob47 said:
Look at this man https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 try and get those... those you have are one petri dish they don't seal you put the agar on the smaller plate and cover it with the bigger one but you will need to pressure cook those dude I really think you need to read more do you have a pressure cooker, a still air box or any of the crucial items we use for agar work? You can't skip corners on agar at all




Man im not noob... i have PC and SAB and all. Im just asking one question at a time about AGAR. i know all other things... So can i use it or not? Waiting other answers plz :mushroom2:


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InvisibleBoogieman47
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Re: Agar question [Re: maestro50]
    #23463378 - 07/21/16 05:37 PM (7 years, 6 months ago)

Well I wasn't sure with some of the questions you're asking ... is it glass?? If so then you can wrap it in foil and PC it with your agar then you will have to take the agar out when it's still warm and pour it into the petri ddish and let it consolidate then drop one or two drops from your syringe on to the agar and wrap the dish with saran wrap and wait


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Offlinemaestro50
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Re: Agar question [Re: Boogieman47]
    #23463578 - 07/21/16 06:33 PM (7 years, 6 months ago)

Ok thanks. Just dont know what do u mean by poruing it into the petri dish when its already in the petri dish. It is glass..

Also. should i somehow clean saran wrap?


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InvisibleBoogieman47
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Re: Agar question [Re: maestro50]
    #23463643 - 07/21/16 06:57 PM (7 years, 6 months ago)

You can't PC the agar in the petri dish you will have to get a wine bottle or something and PC the agar in that wrap the petri dish in foil and PC the with the bottle for agar use the search engine for agar teks it will show you plenty plus those pasty plates I showed you are all reusable and you can PC the agar in those I think it's probably the easiest for a beginner


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InvisibleSupalemonhaze
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Re: Agar question [Re: maestro50]
    #23463689 - 07/21/16 07:13 PM (7 years, 6 months ago)

Quote:

maestro50 said:


Man im not noob... Im just asking one question at a time about AGAR. i know all other things...




Quote:

maestro50 said:
Just dont know what do u mean by poruing it into the petri dish when its already in the petri dish.





:thatsinteresting:


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Offlinemaestro50
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Re: Agar question [Re: Supalemonhaze]
    #23463707 - 07/21/16 07:19 PM (7 years, 6 months ago)

Well i dont know much about agar but learning. Why u take time to quote me but not help me lol. Im learning about pour and no pour right now..

I found this little 120 ml glass containers with metal lid... i think this will work.


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InvisibleBoogieman47
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Re: Agar question [Re: Supalemonhaze]
    #23463708 - 07/21/16 07:19 PM (7 years, 6 months ago)

Haha


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InvisibleBoogieman47
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Re: Agar question [Re: Boogieman47]
    #23463722 - 07/21/16 07:23 PM (7 years, 6 months ago)

Every one gets poked fun at man don't take it personal ... the best thing for you to do is read all the agar teks and people's threads on agar nobody can walk you through it any better then those


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InvisibleJosex
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Re: Agar question [Re: Boogieman47]
    #23463759 - 07/21/16 07:37 PM (7 years, 6 months ago)

Op you're not going to quit asking questions until you have the whole picture.
https://www.shroomery.org/forums/showflat.php/Number/23270429#23270429
Read and watch every single thread in that link, if you still don't get it after that it must mean you're mentally handicaped.


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InvisibleInocuole
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Re: Agar question [Re: Josex]
    #23464161 - 07/21/16 09:53 PM (7 years, 6 months ago)

If you don't want to be laughed at, don't say "I'm not a noob" and then proceed to ask a bunch of noob questions.  Not having basic research skills does make you a noob, so the irony is hard to resist commenting on.

https://www.shroomery.org/forums/showflat.php/Number/22721954


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InvisibleSupalemonhaze
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Re: Agar question [Re: Inocuole]
    #23464706 - 07/22/16 01:15 AM (7 years, 6 months ago)

Which is exactly why I decided to just spectate. :popcorn:


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Offlinemaestro50
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Re: Agar question [Re: Supalemonhaze]
    #23464901 - 07/22/16 05:17 AM (7 years, 6 months ago)

Ok all. I will proceed on this journey by reading more and i guess trial and error. Its easy to get confused and lost in the sea of information but u shouldnt think that i dont read. I always read alot but still have some detailed questions that i dont know. Ill leave this topic so i can ask more questions if i have one later. thanks and cheers


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InvisiblePastywhyteMDiscord
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Re: Agar question [Re: maestro50]
    #23465043 - 07/22/16 07:02 AM (7 years, 6 months ago)

What you need to do is read good teks and start growing. That teaches loads. If you want to learn the finer points then lurk around on the boards and soak up them nuggets as they appear. If someone uses terms you don't understand, do a search on them. Learn what people to listen to and which to ignore. Do that for a year and you will be on good footing.


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OfflineBaronVonBud
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Re: Agar question [Re: Pastywhyte]
    #23465104 - 07/22/16 07:28 AM (7 years, 6 months ago)

You don't have to do transfer to a second agar if using a biopsy from a fresh fruit right ? Couldn't anyone who's never done agar use a sealed 1/2 pint jar with rtf/Tyvek lid and shoot water in when the biopsy grows and they want to use it


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Edited by BaronVonBud (07/22/16 07:29 AM)


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Invisiblemupetmower
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Re: Agar question [Re: BaronVonBud]
    #23465107 - 07/22/16 07:29 AM (7 years, 6 months ago)

yes, you still want to do at least one transfer, even from a plate that was made from a tissue sample. you want to make sure it's clean, and if you made a plate with a clone sample, it will likely not be clean, since we dont fruit in sterile conditions.

and, yeah i guess you could do what youre suggesting, with shooting the water in a SHIP(i think that's what you are talking about?), or you could just make blenderless LI with a plate after you have ensured it' clean with transfers.

there are lots of ways to do things.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleSupalemonhaze
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Re: Agar question [Re: BaronVonBud]
    #23465112 - 07/22/16 07:32 AM (7 years, 6 months ago)

It's always a good idea to make a 2nd transfer when you first inoculate a plate. Clones commonly harbour bacteria. If the clone tissue is contaminated with mold spores, the mycelium will grow over them before they are able to germinate, making a second transfer will get rid of these mold spores whereas if you put them to grains, they will germinate and buttfuck your grain jar.


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