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Offlinemaestro50
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Agar question
    #23448880 - 07/17/16 06:01 AM (7 years, 6 months ago)

Hello all!

Im thinking of getting into agar, and i am reading alot. But one thing is confusing for me..

The purpose of agar is to keep the good mycelium for good growth. And ive seen people cultivate agar with drops from syringe. How would i obtain a good spore? Shouldnt i first try to grow them and then take a tiny piece from inside then put it into petri dish... My question is how to obtain good mycelium or general shroom media to put into agar?


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Invisibleweetsie
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Re: Agar question [Re: maestro50]
    #23448900 - 07/17/16 06:14 AM (7 years, 6 months ago)

Agar is often about clean mycelium.

spores>grain

You get everything that was in the syringe/print growing along side your mushroom mycelium.

spores>agar>grain

Many times mycelium will outrun contaminants that were in with the spores, bacteria is very slow to move across an agar plate so taking mycelium from the edge of the growth means you're not inoculating your grain with it.

Quote:

Shouldnt i first try to grow them and then take a tiny piece from inside then put it into petri dish... My question is how to obtain good mycelium or general shroom media to put into agar?




No. If you're starting from spores, put them on agar, select aggressive and clean mushroom mycelium to transfer to your grain. After you get fruits then of course cloning on agar for your next grow is a good way to go.

Basically everything starts on agar, If I use spores they go on agar, If I clone I clone on agar, if someone sends me an edible wedge of agar, it goes on agar first to make sure it's clean.


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Offlinemaestro50
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Re: Agar question [Re: weetsie]
    #23450526 - 07/17/16 05:08 PM (7 years, 6 months ago)

Thanks man and i have few more questions. agar really seems interesting and also funn to do. and ofcourse is next step in growing beauties.

First, what do u mean by select aggressive and clean mushroom mycelium to transfer to grain? I get the spore print, then i apply a bit of it to agar agar and in the same time i grow the mushroom to check if the potency is good? Then if its good i have that spore in agar developing, and then take good white stuff from the edge right?


Really starting this so noob :crazy2:


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Invisiblemupetmower
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Re: Agar question [Re: maestro50] * 1
    #23450987 - 07/17/16 07:52 PM (7 years, 6 months ago)

this thread is literally right above yours. read that, as well as lot of other teks/info on agar.

Pasty Plates is great, too.

there are 100's of them.

Trusted Cultivator's agar links too.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (07/17/16 07:53 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Agar question [Re: mupetmower]
    #23451035 - 07/17/16 08:05 PM (7 years, 6 months ago)

:whathesaid:

Last link in my signature


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Invisibleweetsie
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Re: Agar question [Re: maestro50]
    #23451122 - 07/17/16 08:33 PM (7 years, 6 months ago)

Quote:

maestro50 said:
First, what do u mean by select aggressive and clean mushroom mycelium to transfer to grain?





When you put spores on agar you will get potentially thousands of strains growing, some of it will appear as fast rhizomorphic mycelium and some will be slow and weak looking. Selecting the former guarantees you nothing but can certainly improve your odds of success.

Quote:

maestro50 said:
I get the spore print, then i apply a bit of it to agar agar and in the same time i grow the mushroom to check if the potency is good?





No, every spore print will contain thousands of spores which will make thousands of strains. Most of them will be okay, some will be useless and some will be amazing.

Using the print and getting lucky will a good yield of potent shrooms means very little, the next time you use that print you could get barely active junk.

Watch RR's agar videos, they make it clearer than I will ever be able to explain.


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Offlinemaestro50
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Re: Agar question [Re: mupetmower]
    #23453732 - 07/18/16 05:34 PM (7 years, 6 months ago)

I know and i read. Everybody read i guess, but the specific question is good to ask and is always nice to get answer u want... Some details are not always said... :tongue:


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Offlinemaestro50
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Re: Agar question [Re: weetsie]
    #23453737 - 07/18/16 05:36 PM (7 years, 6 months ago)

Quote:

maestro50 said:
I get the spore print, then i apply a bit of it to agar agar and in the same time i grow the mushroom to check if the potency is good?





No, every spore print will contain thousands of spores which will make thousands of strains. Most of them will be okay, some will be useless and some will be amazing.

Using the print and getting lucky will a good yield of potent shrooms means very little, the next time you use that print you could get barely active junk.

Watch RR's agar videos, they make it clearer than I will ever be able to explain.




So how do i select the best spore? dont understand at all how to cultivate the best spore from the print on agar

U said i DONT put a little spore on agar? from where do i get mycelium than. pls help confused hehe


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InvisiblebodhisattaMDiscordReddit
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Re: Agar question [Re: maestro50]
    #23453756 - 07/18/16 05:44 PM (7 years, 6 months ago)



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InvisiblePastywhyteMDiscord
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Re: Agar question [Re: maestro50]
    #23453769 - 07/18/16 05:48 PM (7 years, 6 months ago)

Use the agar to find healthy strong growth. You can then either continue to transfer until you have an isolate or do an ms grow. If you grow from ms you can then clone any fruits that look good.

Once clones or isolates are obtained you then do a test grow of each to see if it's got everything you want, potency, yield, etc. You must do a test grow, simply having a clone or an isolate is guarantee of anything (though with a clone at least you know it can fruit).
Most isolates tend to suck with 10% being really exceptional and 1% being stellar. Clones are a little more average but if you took 100 clones, for sure there would be 5 or 6 that were amazing.

Once you have your desired genetics you then slant the masters and grow the clone or isolate for the next 20 years. No more spores, unless you want to start over, then you simply take a print.


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Offlinemaestro50
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Re: Agar question [Re: Pastywhyte]
    #23455389 - 07/19/16 08:56 AM (7 years, 6 months ago)

Guys i appreciate your respond but u didnt answer my question again! If i dont understand this part my agar adventure will dissapear now...

Pasty u are talking about obtaining an extraordinary agar situation but again how do i get there. I asked u if u put a bit of spore print on agar and in the same time grow batch of same print so i can see the quality... and u said no. So STARTING from spore print... How do i make fertile agar culture? Ive read all links and searched bunch but still dont know this first basic step. helpzzzz


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Re: Agar question [Re: maestro50]
    #23455516 - 07/19/16 09:38 AM (7 years, 6 months ago)

What do you mean I said no? Either put a drop of spore solution or streak spores on the plate with a sterile loop or scalpel blade or something. Then let them germinate and grow. I'm not sure what else to tell you man.


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Invisiblemupetmower
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Re: Agar question [Re: Pastywhyte]
    #23455548 - 07/19/16 09:47 AM (7 years, 6 months ago)

i think he is asking if he can inoc some plates, as well as inoc some brf(or something) to grow and fruit along side the plates, to test their potency.

you can, but it's MS, which means you wont know for sure that the fruits will end up the same from the ones you grow to the ones you are making plates of. so what pasty is saying is to make the plates, grow them out, make transfers, picking the best growth, then eventually inoc with the plates to test the culture, while even keeping a master in case its an amazing culture.


Pasty can definitely explain better. i have a hard time getting what i mean out.


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-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: Agar question [Re: mupetmower]
    #23455565 - 07/19/16 09:53 AM (7 years, 6 months ago)

Okay if he is talking about knocking up something with the spores in the meantime then yeah why not? Just remember that contamination is a risk when you do that.

Potency is genetic. Spores are a genetic gamble. I can do 10 grows from spores and have different results every time.


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Offlinemaestro50
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Re: Agar question [Re: Pastywhyte]
    #23460323 - 07/20/16 06:10 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Okay if he is talking about knocking up something with the spores in the meantime then yeah why not? Just remember that contamination is a risk when you do that.

Potency is genetic. Spores are a genetic gamble. I can do 10 grows from spores and have different results every time.




Im still confused. More than before lol. Let me sum it up a bit.

So i have my agar agar ready. And i have spore print, or spore syringe. So what do i do now? Lets say i dont yet grow shrooms, BRF cakes. I only have my spores on agar and waiting for mycelium? If that is right, how do i cultivate the mycelium culture to reach the quality u are talking about?

Mupetmower said:

1make the plates- Make agar formula in plates... right?

2grow them out- Wait for mycelium to advace...right?

3make transfers, picking the best growth, then eventually inoc with the plates to test the culture, while even keeping a master in case its an amazing culture.- This i dont understand... Make transfer from one agar plate to another... What will i achieve with it? And ofcourse... what does, picking the best growth means... how do i spot the best myc? And lastly, what do u mean when u said inoc with the plates to test culture... Inoculate cakes with the whole agar plate with myc on, then growing them and testing?

Bare with me guys... I feel really bad, im feeling like bothering u but its really not clear to me. :sun:


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InvisiblePastywhyteMDiscord
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Re: Agar question [Re: maestro50]
    #23460351 - 07/20/16 06:18 PM (7 years, 6 months ago)

Okay here I will try once more.

Put spores on agar plate. Wait for growth.

Once you have growth cut a tiny rice sized piece (wedge) from the plate and put on a new plate. Wait for that to grow.

Observe new growth. If it looks clean you can then either transfer another piece to a new plate or several plates, or you can put a decent sized wedge into a grain jar.

Always choose healthy fast areas to cut your transfer pieces from. That will ensure the culture is the best available.


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InvisibleInocuole
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Re: Agar question [Re: Pastywhyte]
    #23460718 - 07/20/16 08:53 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Observe new growth. If it looks clean you can then either transfer another piece to a new plate or several plates, or you can put a decent sized wedge into a grain jar.




I do both. :shrug:


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InvisibleBoogieman47
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Re: Agar question [Re: Inocuole]
    #23461443 - 07/21/16 02:18 AM (7 years, 6 months ago)

I think the dude is confused about what specific spore to put on agar ... each spore is microscopic you will put alot of spores on agar it's not just one plus spores need to germinate with other spores so basically the spores have sex and produce the white mycelium ....


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Edited by Boogieman47 (07/21/16 02:21 AM)


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Re: Agar question [Re: Boogieman47] * 1
    #23461654 - 07/21/16 05:04 AM (7 years, 6 months ago)

I think that the first thing OP should do is look at some agar threads. You guys are trying to explain things which he has never even seen, let alone know what they would look like.

Here is something that can (maybe, but just maybe) help you out OP:

Step one, spore inoculation:
Basically, you do what pasty said, use any flame sterilized tool to scrape some spores from your print and put those on your agar (in a SAB, of course). If using spore syringe, just flame the needle and drop 1 or 2 drops:




Step two, waiting for the magic:

This is germination under a microscope, you can see mycelium started to grow from that single spore:


This is what germination will look like on a plate, you can see the mycelium growing under the surface of the agar:



Step three, transfers, clean growth, inoculation:

After spore germination pictured above, you need to do at least 2 transfers to ensure your culture is clean and also to allow enough time for mycelium to start showing you if it is organized or not. Organized mycelium has the best chance of being contam free, that is what you want.

This is organized and clean mycelium obtained after 2 transfers:


Once you get this organized mycelium, you can cut off wedges and drop them in your grain jars. You can use any sized wedge, the bigger the wedge/more wedges you throw in there, the faster colonization happens. I usually transfer wedges the size of 1/4 of the plate at a time, I will use 1 or 2 of these 1/4 plate wedges on a single jar.

This was more work than I thought it was going to be so I sure as shit hope you understand now OP. Regardless of if you think you understand or not, you still want to do more research. You can never learn enough in mycology.


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Offlinemaestro50
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Re: Agar question [Re: Supalemonhaze]
    #23461746 - 07/21/16 06:30 AM (7 years, 6 months ago)

Thanks guy a bunch! I really love this forum and appreciate the effort. I now understand what it is all about!

Ive also heard that the mycelium is faster then the bacteria... So the bacteria will evolve in the middle and the good mycelium will grow faster to the edges. So when im transfering the wedge i always took the most advanced one to the edge?

I have some syringes in my refrigerator for about two months so i will sure try it this week. Looking forward to it! The idea of obtaining perf. myc. this way seems logic and funn. One more thing... Where do i keep plates? cool and dark place?


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