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Tex3000
Shroom rookie


Registered: 06/04/16
Posts: 37
Loc: Mass
Last seen: 2 years, 10 months
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Problem with contamination
#23445161 - 07/15/16 08:25 PM (7 years, 6 months ago) |
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My first run of 12 jars were clean. My second run I went up to 24 jars and about half of them got contaminated with what I'm guessing was a bacteria. It's almost like the wbs started to rot and the smell is very sweet and very terrible. I washed the jars real good and for this 3rd run I went with 24 jars and probably 10 fell out to the same contamination. I figured the pc would destroy anything I didn't get with the wash. Is it possible I'm carrying this bacteria over everytime? Should I pc the jars that are contaminated (pc them empty in the pressure cooker)? While it is frustrating losing jars, the worst part by far is the smell, it is almost unbearable. Any help would be greatly appreciated. I do a 24 hour soak, simmer for 20, dry it until it is dry to the touch, jar them up, pc for an hour, then cool and inoculate. Some of the contaminated jars are still growing which I find is odd. I usually do the smell test of the visual test is too tough to figure out.
Edited by Tex3000 (07/15/16 08:34 PM)
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Mushierage
SWIM Sinker


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Problem with contamination [Re: Tex3000]
#23445180 - 07/15/16 08:35 PM (7 years, 6 months ago) |
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Your grains are wayyyyy too wet. Work on your grain prep a bit. Anything that got contaminated needs thrown out. You can reuse the jars and PC them, don't reuse the grain/WBS for obvious reasons.
Also, WBS is notorious for endospores. It's really really dirty shit man. PC for 90 minutes, not 60 minutes. This will kill any germinated endospores and bacterial short comings.
Also I don't see any contamination in the pic you show but maybe I'm just not seeing it with These old eyes? Anyway, if it isn't, the wetness of the jar is surely to contaminate.
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Tex3000
Shroom rookie


Registered: 06/04/16
Posts: 37
Loc: Mass
Last seen: 2 years, 10 months
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Re: Problem with contamination [Re: Mushierage]
#23445194 - 07/15/16 08:41 PM (7 years, 6 months ago) |
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Quote:
Mushierage said: Your grains are wayyyyy too wet. Work on your grain prep a bit. Anything that got contaminated needs thrown out. You can reuse the jars and PC them, don't reuse the grain/WBS for obvious reasons.
Also, WBS is notorious for endospores. It's really really dirty shit man. PC for 90 minutes, not 60 minutes. This will kill any germinated endospores and bacterial short comings.
Also I don't see any contamination in the pic you show but maybe I'm just not seeing it with These old eyes? Anyway, if it isn't, the wetness of the jar is surely to contaminate.
Thanks for the reply. I will increase my pressure cooking time. The contamination isn't a visible thing, only way I know is by the terrible smell and the stalled growth. I'm not entirely sure how to get the grains drier, I use a fan, towel, paper towels, etc. they don't enter a jar until they are dry on the outside. Maybe water is getting in the jars when I pc them? Even after pcing them when I'm doing the g2g transfer I'll take a paper towel and dry off the top of the jars and the inside of the covers. This hobby really isn't a hobby that you can cut any corners!
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Mushierage
SWIM Sinker


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Problem with contamination [Re: Tex3000]
#23445217 - 07/15/16 08:54 PM (7 years, 6 months ago) |
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Yep. The sweet smell is a bacterial infection breaking down your grain. Probably bacillus. I might try skipping the simmer process but you may want to wait for another reply from somebody that's worked with WBS more than I have. I've only used WBS a couple times and have had hit and miss success with it.
Cheers.
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Problem with contamination [Re: Mushierage]
#23445283 - 07/15/16 09:30 PM (7 years, 6 months ago) |
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Your grains are too wet externally. Increase your times to 90+ min. I do 120 @ 18 psi..
It's warmer seasons and condensation is an issue. Condensation and excess water leads to your grain unable to colonize. The grain ferments and bacteria and yeast grows. Sometimes if I see too much water in my jars I'll have to give it a shake to distribute the moisture
Up PC time - completely dry grain - watch out for condensation - up sterile procedure
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
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Re: Problem with contamination [Re: Lobi]
#23445334 - 07/15/16 09:50 PM (7 years, 6 months ago) |
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I'm guessing you are inoculating your grains with spores, and that your spore syringes all came from the same place, and that they aren't at all clean. If you want to avoid bacteria in your grain jars investigate clean spawn creation via agar work. Good luck!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Fruit Cakes
Shroom-God

Registered: 06/30/16
Posts: 61
Last seen: 7 years, 6 months
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Re: Problem with contamination [Re: Lobi]
#23445338 - 07/15/16 09:54 PM (7 years, 6 months ago) |
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was reading these post and now i would like to ask, what would i do for Rye grain that succesfully colonized a jar... I now need to case it and was wondering if I should use vermiculite and if i do should i wet it just a tad bit? P.S i am noob
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Mushierage
SWIM Sinker


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Problem with contamination [Re: Fruit Cakes]
#23445347 - 07/15/16 09:59 PM (7 years, 6 months ago) |
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Spawn it to a bulk substrate and the. Let that colonize.
Some people say you can case grains and fruit right from it. I personally am not a fan because of how easily it contaminates. I would spawn to bulk, colonize, case, and then fruit in trays in an SGFC or a monotub.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
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Re: Problem with contamination [Re: Fruit Cakes]
#23445349 - 07/15/16 09:59 PM (7 years, 6 months ago) |
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well, you could use it to inoculate a few more jars of rye then make a monotub. Or you could mix it with a quarter brick of pasteurized hydrated coir and about .75qts vermiculite (it would be about 1.25quarts water to hydrate) and spawn it in a mini monotub....a 15qt tote would do fine.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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