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towelie2016
Your Highness

Registered: 07/10/16
Posts: 16
Last seen: 7 years, 5 months
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Attempt to grow with cardboard tek and dry tissue (pics)
#23444458 - 07/15/16 04:21 PM (7 years, 7 months ago) |
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Hi guys, I recently got back in the hobby and would like to grow. My problem is there's no way for me to obtain spores either from wild specimens (I've never found any) or through our sponsor because of where I am at. Luckily for me I was able to obtain a few dry specimens (it's extremely hard to find any around here) and I decided I'd give the cardboard tek a go after reading so much about it.
What I did: _ Cardboard is cut from shipping box. 1"x1". soak in water. Peel in half. Save corrugated part, discard other (per cardboard tek). _ Boil piece of cardboard in tap water for 10 min. Let cool completely. Use sanitized tweezers to transfer cardboard to glass jar (allow cardboard to drain a bit so it is not over saturated w/water). _ Mask, bodysuit, Clean working surface w/ iso, no glovebox just no far nor A/C. _ Make polyfill hole in jar lid and wipe jar w/ iso. _ Use gloved hands to break up a piece of tissue 1 inch long and half inch above stem. Drop tissue in cooled boiled water used to boil cardboard to re-hydrate for a minute or two. Use tweezers to transfer re-hydrated tissue to jar, onto cardboard. Close lid and wait.
Pics 7/15 Garlic jar with dry tissue on wet cardboard. 2 days old.

A piece of gill was collected and transferred to a different jar w/ same tek. To see if it will germinate at all?
 The re-hydrated tissue turned yellow and blue and brownish and looks like dead or rotten tissue to me. But I've seen other threads where the tissue turned the same way before turning into white mycelium and colonizing. I'm hoping that the dry tissue will revive and start colonizing the whole cardboard.
Then what would be my best bet? Try to clean up with agar? I can't order petri dishes (reluctant to use debit card/info). I can try to find more tiny jars like the one I have and use agar, if I absolutely need to speed up the process (instead of ruining spawn with contamination, then having to start again). If I can transfer the colonized cardboard directly to a small jar of grain without much risk, I'd rather not use agar because I'm only working with a little amount of time and space.
What is you guys' take on it?
Edit: The species was cubes. Sorry I forgot.
Edited by towelie2016 (07/15/16 06:24 PM)
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: towelie2016]
#23444500 - 07/15/16 04:32 PM (7 years, 7 months ago) |
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i highly doubt this is going to work, honestly. what species is this even supposed to be? and you wont be able to transfer that colonized cardboard, if that ever even would happen, to grain, without any risk, because none of what you are working with is sterile. none of it.
good luck, but honestly. i think you are just wasting your time. you probably wouldve had a little more luck just dropping the tissue onto an agar plate, but sine its been dried, i doubt that would even work at all, either.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: mupetmower]
#23444521 - 07/15/16 04:39 PM (7 years, 7 months ago) |
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If you have dried mushrooms just take a spore swab, saves you trying to reanimate long dried tissue which while possible is hard, especially the way you're going about it.
You don't need petri dishes to do agar either.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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towelie2016
Your Highness

Registered: 07/10/16
Posts: 16
Last seen: 7 years, 5 months
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: weetsie]
#23444863 - 07/15/16 06:28 PM (7 years, 7 months ago) |
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Quote:
mupetmower said: i highly doubt this is going to work, honestly. what species is this even supposed to be? and you wont be able to transfer that colonized cardboard, if that ever even would happen, to grain, without any risk, because none of what you are working with is sterile. none of it.
good luck, but honestly. i think you are just wasting your time. you probably wouldve had a little more luck just dropping the tissue onto an agar plate, but sine its been dried, i doubt that would even work at all, either.
If the dry tissue revives and colonizes the cardboard, and transfer to grain is not worth the time, then what will I do with the mycelium? Transfer to a small amount of pasteurized straw or poo? wouldn't that be a better shot? After all I only need one clean fresh fruit to get print right?
Quote:
weetsie said: If you have dried mushrooms just take a spore swab, saves you trying to reanimate long dried tissue which while possible is hard, especially the way you're going about it.
You don't need petri dishes to do agar either.
I'm going to do that too. The cardboard tek was done before I obtained a 23qt. Presto. I will cook up some agar jars once I make a glove box. It's a fresh start for me. Thanks, guys.
Edited by towelie2016 (07/15/16 06:35 PM)
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: towelie2016]
#23444874 - 07/15/16 06:31 PM (7 years, 7 months ago) |
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to have any shot with it, you basically need agar. otherwise, you are just going to contaminate the grains and other stuff with it.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Psilocamel
Propogate-er


Registered: 05/10/16
Posts: 117
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: mupetmower]
#23447787 - 07/16/16 08:30 PM (7 years, 7 months ago) |
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Use pasty plates. No Petri's. With out agar you are wasting time. I would seriously consider agar. With a spore swab. And with with dried tissue Expect to fight contams at every transfer
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Mushiez


Registered: 04/28/14
Posts: 1,057
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: towelie2016]
#23447843 - 07/16/16 08:57 PM (7 years, 7 months ago) |
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P. cubensis doesn't do well on cellulose. It prefers sugars, starches, aka agar media
The tissue looks to be bacterially contaminated from the photo
With less moisture, this method would work for P. ostreatus or P. azurescens
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towelie2016
Your Highness

Registered: 07/10/16
Posts: 16
Last seen: 7 years, 5 months
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: Psilocamel]
#23453302 - 07/18/16 03:24 PM (7 years, 7 months ago) |
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Quote:
Psilocamel said: Use pasty plates. No Petri's. With out agar you are wasting time. I would seriously consider agar. With a spore swab. And with with dried tissue Expect to fight contams at every transfer
Yeah. I don't have a flow hood atm, only a glove box to be built soon, so I'm really reluctant to use agar, though I've done agar in a glove box before with success. My guts tell me this has a better chance of success than agar (I also have limited time and space and even less tool), so I'm trying this method out first. If all else fails then agar it is.
Quote:
Mushiez said: P. cubensis doesn't do well on cellulose. It prefers sugars, starches, aka agar media
The tissue looks to be bacterially contaminated from the photo
With less moisture, this method would work for P. ostreatus or P. azurescens
I'm not sure. I've seen others try growing on shredded leaves before. It seems Cubes can grow on leaves just fine. Not so much so with woodchips. I imagine that the cardboard only acts as a medium with little to no nutes. I'm hoping the mycelium will colinize the whole piece, and eat or defeat all the contaminants in there before I spawn it to some more substrate. Too unrealistic?
Anyways, it's been 4 days. I have some progress.
The piece of gill that I dropped on wet cardboard has started to colonize the cardboard. I'm thinking that is just mycelium reviving and not spores germinating.

The piece of dry tissue from base of the stem is turning white and fuzzy as well. though at a lower rate. Could be because one is a quart jar and the other is a 1/4 pint garlic jar. or some unknown reason I'm not sure.

If either jar's cardboard is fully colonize, which I think it should from reading others' work, I'm going to spawn the piece of cardboard to a half pint jar of pasteurized straw, let colonize and attempt to fruit to obtain spores, or maybe spawn to a little more bulk substrate to multiply. My understanding to that using bulk substrate will dramatically reduces risk of contamination compared to using grain. I figure if I keep multiplying mycelium by spawning to small amount of bulk substrate, eventually I'll have enough spawn (a quart jar worth?) to fruit them and have decent flush.
Decent flush => spores => bulk project
I will keep this post updated.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 5 months
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: towelie2016]
#23453335 - 07/18/16 03:38 PM (7 years, 7 months ago) |
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Mattrus
Stranger


Registered: 10/31/17
Posts: 41
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Re: Attempt to grow with cardboard tek and dry tissue (pics) [Re: towelie2016]
#24821302 - 12/02/17 01:23 PM (6 years, 2 months ago) |
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When the last thing posted on an old fourm is "ill keep post updated" with no updates.
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