Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinefrankwhite85
Stranger


Registered: 04/18/16
Posts: 305
Last seen: 1 month, 6 days
11 rounds of transfers shouldn't i be seeing sectoring by now?
    #23441829 - 07/14/16 07:45 PM (7 years, 6 months ago)

So i started an escondido culture from spores on agar and these are the result of about 11 rounds of transfers and i feel like i'm not seeing defined sectoring like i should be by now.  Any thoughts?

dish 1


dish 2


dish 3


dish 4


dish 5


Extras: Filter Print Post Top
InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: 11 rounds of transfers shouldn't i be seeing sectoring by now? [Re: frankwhite85]
    #23441934 - 07/14/16 08:15 PM (7 years, 6 months ago)

You probably should've used them like 9 transfers ago...but then again I'm not exactly sure what you're really looking for specifically...they look fine to me? :shrug:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: 11 rounds of transfers shouldn't i be seeing sectoring by now? [Re: Kush_Zombie]
    #23441989 - 07/14/16 08:31 PM (7 years, 6 months ago)

Sectoring appears on the first plate if you let it grow out a bit, I don't think I have ever not had sectoring by the second plate.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
Re: 11 rounds of transfers shouldn't i be seeing sectoring by now? [Re: weetsie]
    #23442104 - 07/14/16 09:13 PM (7 years, 6 months ago)

I'm curious to hear what others have to say about this as well.. I'm on my 5th round of transfers of one strain right now and they are all looking similar to yours. I just tried a slightly less nutritious mix of agar this round to try to promote more rhizo and hopefully sectoring, maybe you could benefit from trying that as well :shrug:


--------------------
:vaped: Highwalker :bongload:


Extras: Filter Print Post Top
Offlinefrankwhite85
Stranger


Registered: 04/18/16
Posts: 305
Last seen: 1 month, 6 days
Re: 11 rounds of transfers shouldn't i be seeing sectoring by now? [Re: lukehighwalker710]
    #23442159 - 07/14/16 09:29 PM (7 years, 6 months ago)

I use a pretty low ratio, 6 grams of malt extract to 12 grams agar.  I'm sure they would do ok if i just put em to grain but im really trying to get monocultures down.


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: 11 rounds of transfers shouldn't i be seeing sectoring by now? [Re: frankwhite85]
    #23442214 - 07/14/16 09:49 PM (7 years, 6 months ago)

If you took say wedges 1/16th of the outer growth on each plate and used all 16 wedges to make new plates each time you would end up with over 1 million plates by the 6th transfer. If you have done 11 transfers, you will have monocultures.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
Invisibledankington
The Stranger
Male User Gallery


Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: 11 rounds of transfers shouldn't i be seeing sectoring by now? [Re: weetsie]
    #23442258 - 07/14/16 10:02 PM (7 years, 6 months ago)

Also, it appears you are taking large transfers.
You have some really beautiful growth! If you really want isolates, you must take small transfers--as small as you can. Like a dry grain of rice.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* agar to agar transfer ohmatic 15,622 11 01/12/21 09:00 PM
by bodhisatta
* Can you avoid G2G senescence by transferring flesh to Agar? sasquatchisreal 908 11 03/20/18 01:10 PM
by bodhisatta
* Protecting Monoculture from Sceanecence pftek 1,745 7 09/02/09 08:14 PM
by Sebastien
* Agar plates should i transfer or make lc baddagger 907 4 03/13/18 11:42 AM
by baddagger
* Monocultures.
( 1 2 all )
badman 2,675 20 07/31/09 04:25 PM
by badman
* Does cloning give you a monoculture? meehi 3,981 6 03/29/09 04:19 PM
by Shroominit
* Cake 2 Cake transfer questions hankfu 1,220 3 03/18/05 06:06 AM
by Anything4Poppies
* transferring mycelium wikedanjel 4,113 13 01/10/03 03:54 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
243 topic views. 23 members, 192 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 14 queries.