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Invisiblec10h12n2o
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Old Liquid Culture [pics]
    #23441016 - 07/14/16 02:13 PM (7 years, 6 months ago)

hi everyone, i hope your summer is turning out lovely :smile:

so, I was doing some cleaning and found a couple of liquid cultures from hawks eye that i had forgotten about.

They are a little over a year old, and have been stored at room temp. Species are lions mane, shaggy mane, and an oyster. concurrently stored spore syringes have had 0 visibe contams on hundreds of plates so far.

These were originally much different colors, and it would be my guess that they are dead and/or bacteria-ridden, but i wanted to get others' opinions. Should I try them on agar and isolate any myc away from any contams? or any other suggestions? thanks everyone!!


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OfflineTheDeathryderIsBac
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Re: Old Liquid Culture [pics] [Re: c10h12n2o]
    #23441045 - 07/14/16 02:24 PM (7 years, 6 months ago)

Most likely bad by now.  Most people don't store LC's longer than 6 months at the most with refridgeration.


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Invisiblec10h12n2o
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Re: Old Liquid Culture [pics] [Re: TheDeathryderIsBac]
    #23441108 - 07/14/16 02:47 PM (7 years, 6 months ago)

right, my thoughts exactly. I had literally forgotten about these haha..

should i just assume these are bad and throw them away and plan to order some more?

if i have agar to spare is there any way to attempt to save any?


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Offlinelukehighwalker710
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Re: Old Liquid Culture [pics] [Re: c10h12n2o]
    #23441207 - 07/14/16 03:43 PM (7 years, 6 months ago)

I don't know enough to say one way or the other whether those are still viable, but there's no harm in trying em out :shrug: put a drop of each onto some plates and see what happens. You'll know what's up with them in a few days..


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InvisibleMunchauzen
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Re: Old Liquid Culture [pics] [Re: c10h12n2o]
    #23441280 - 07/14/16 04:20 PM (7 years, 6 months ago)

Quote:

c10h12n2o said:
right, my thoughts exactly. I had literally forgotten about these haha..

should i just assume these are bad and throw them away and plan to order some more?

if i have agar to spare is there any way to attempt to save any?



its not much of a hassle to put em on agar and find out.... I would at least try them.


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Invisiblec10h12n2o
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Re: Old Liquid Culture [pics] [Re: Munchauzen]
    #23466776 - 07/22/16 05:18 PM (7 years, 6 months ago)

UPDATE:

so I took a sterile loop and caught some tissue from each of the 3 cultures and did 4 plates for each

in every case, 50% show no growth, and 50% have some myc, what kind is yet to be determined

Here are some pics from today:

Shaggy Mane
Plate A

Plate B

Lions Mane
Plate A

Plate C

Blue Oyster
Plate A

Plate D


Obviously the Blue Oyster looks suspect (some kind of sporophore?), but the others might have the right myc, or maybe it will turn out to be the same ugliness lol....

I am not familiar with any of these species besides reading, and they are from the old and brown LCs in OP. Im suprised i am not seeing more bacteria.

Since i havent worked with these species before, i figured i would ask what others think. Is there anything here worth trying to isolate away from contams? or is it all  mold? i would be highly surprised and impressed if anything good came out of those old cultures i found in my garage lol


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (07/22/16 05:19 PM)


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