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OfflineShroomsh
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Registered: 07/28/15
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Instant Pot for sterilization? [UPDATE: so far so good!] pics
    #23440920 - 07/14/16 01:36 PM (7 years, 6 months ago)

I just recently purchased an Instant Pot , and I am curious if anyone has experience with or knows if this will work just as well as a pressure cooker. 

I know it's probably a stupid question, but I've never used a pressure cooker before.  My previous sterilizing method was just basically steaming the jars.


UPDATE: 7/26/16

So it worked!  I used the instant pot, steam setting, high pressure, total of 60-80min.  This involved several times of releasing pressure and running the pot again. 

All 6 of these jars below were sterilized in the instant pot.  You'll notice that a few of them are just barely starting out now, and really that is due to me reeeaalllly stretching my spore solution across all my jars.  But hey, some of these jars are just now showing signs of growth!  I've been waiting to see that! 



Also as you see, I have half of the IP jars in the closet and half are in an incubator.  I'm not even sure if that incubator is working...I really hate these tetra water heaters. 


Edited by Shroomsh (07/26/16 12:13 PM)


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Offlineguyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
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Re: Instant Pot for sterilization? [Re: Shroomsh]
    #23440949 - 07/14/16 01:47 PM (7 years, 6 months ago)

it won't get above 11psi, so you will have to increase your time tremendously.

It will be better than steaming I guess.


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OfflineMorel Guy
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Re: Instant Pot for sterilization? [Re: Shroomsh]
    #23440958 - 07/14/16 01:51 PM (7 years, 6 months ago)

I looked into these and the nuwave pressure cooker.  It's not recommended to do canning in these devices.  It would be nice to have set and forget canners.  Not a fan myself of babysitting the cooker.  It's probably the worst part.


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: Instant Pot for sterilization? [Re: Morel Guy]
    #23440992 - 07/14/16 02:04 PM (7 years, 6 months ago)

Damn dude, for that price you could've gotten the presto 23 quart pressure AND another bag of rye (or whatever you use) AND backup seals for the pressure cooker.

Pressure cookers are easy man. Can't answer your question though, I wouldn't use it personally.  It'd work well for brf cakes though.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineShroomsh
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Registered: 07/28/15
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Re: Instant Pot for sterilization? [Re: Kush_Zombie]
    #23442558 - 07/14/16 11:52 PM (7 years, 6 months ago)

Mmm, that's too bad.  I'll see how it works anyway, hopefully it won't be a waste of time.  I do want to say though, I did not buy this instant pot for sterilization.  I bought it primarily for food, but I did want to see about sterilization as well. 

Ah well, I'll give it a go and if it doesn't work out I'll cry and go see if I can find a cooker at goodwill or something.


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
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Re: Instant Pot for sterilization? [Re: Shroomsh]
    #23442905 - 07/15/16 04:00 AM (7 years, 6 months ago)

That thing will suck balls for mycology. You won't be able to do anything worthwhile with 6qts of volume. That was a waste of some perfectly good money which could have went towards a proper PC. You could have used said PC for food anyway.


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OfflineShroomsh
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Registered: 07/28/15
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Re: Instant Pot for sterilization? [Re: Supalemonhaze]
    #23443796 - 07/15/16 12:16 PM (7 years, 6 months ago)

Oh well!  Guess it's time to experiment!


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OfflineShroomsh
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] [Re: Shroomsh]
    #23478504 - 07/26/16 12:00 PM (7 years, 6 months ago)

Updated with pics!


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Offlineblackout
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] [Re: Shroomsh]
    #23479052 - 07/26/16 02:59 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Updated with pics!



you are supposed to take the foil off the jars. I presume you have a dry layer of verm at the top? this acts as a filter.

If you do not have a dry layer I would leave the foil on, but maybe loosen it a little.

If left on the jars cannot breathe properly and the top layer can become moist when it should be pretty dry, it will be prone to contaminate if moist.

You should move the jars as little as possible, and always hold them upright, they look tilted, this would move the dry verm layer around.


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InvisibleInocuole
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Re: Instant Pot for sterilization? [Re: Supalemonhaze]
    #23479173 - 07/26/16 03:47 PM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
That thing will suck balls for mycology. You won't be able to do anything worthwhile with 6qts of volume. That was a waste of some perfectly good money which could have went towards a proper PC. You could have used said PC for food anyway.




Note the hall of shame status.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: Instant Pot for sterilization? [Re: Inocuole]
    #23479177 - 07/26/16 03:49 PM (7 years, 6 months ago)

Can't hate the guy for getting results but he really should have spent the money on a PC still imo


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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InvisibleInocuole
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Re: Instant Pot for sterilization? [Re: Kush_Zombie]
    #23479185 - 07/26/16 03:52 PM (7 years, 6 months ago)

Are we ignoring that that is WAY too early to call it "getting results"?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: Instant Pot for sterilization? [Re: Inocuole]
    #23479191 - 07/26/16 03:55 PM (7 years, 6 months ago)

Oh, I misunderstood what you were saying. I was saying that meaning to agree with you :lol:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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InvisibleKenInVic
Hey Bulldog
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Registered: 03/01/16
Posts: 1,452
Loc: 3rd Stone from the Sun Flag
Re: Instant Pot for sterilization? [Re: Inocuole]
    #23479195 - 07/26/16 03:57 PM (7 years, 6 months ago)

Quote:

Inocuole said:
Are we ignoring that that is WAY too early to call it "getting results"?




Their jars are stalled and they want to re-inoculate with a different strain in another thread.  Sounded like the foil was still in play as well.


--------------------
***My SGFC***  ***ID Mushrooms Here***
Pondering the question, "Are we all here, because we're not all there?"
       

"Because something is happening here, but you don't know what it is, do you, Mr. Jones."  Ballad of a Thin Man by Mrs. Zimmerman's little boy, Bobby.


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InvisibleMad Season
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Re: Instant Pot for sterilization? [Re: KenInVic]
    #23479222 - 07/26/16 04:08 PM (7 years, 6 months ago)

2 front page shroomsh questions. 2 fail threads. Coincidence?


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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OfflineShroomsh
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Registered: 07/28/15
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] [Re: blackout]
    #23482374 - 07/27/16 01:27 PM (7 years, 6 months ago)

Quote:

blackout said:
Quote:

Shroomsh said:
Updated with pics!



you are supposed to take the foil off the jars. I presume you have a dry layer of verm at the top? this acts as a filter.

If you do not have a dry layer I would leave the foil on, but maybe loosen it a little.

If left on the jars cannot breathe properly and the top layer can become moist when it should be pretty dry, it will be prone to contaminate if moist.

You should move the jars as little as possible, and always hold them upright, they look tilted, this would move the dry verm layer around.




This is tek I use: http://www.fungifun.org/English/Pftek

At the bottom after the inoculation instructions it says to put the second layer of foil back on the jars.  Is this information incorrect?  I do have a layer of verm on top, but I have not heard of keeping air holes open for the jars before.  I would assume it's not too late to go do that? 


Also for everyone saying that the instant pot was a waste of money, I was buying it for food.  I did not make this purchase for mushroom cultivation at all.


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Invisiblemupetmower
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] [Re: Shroomsh]
    #23482501 - 07/27/16 02:25 PM (7 years, 6 months ago)

if it has a verm layer and a lid then it doesnt need the foil. the foil might even hurt it by making moisture build up and fuck up the verm layer. so yeah, i would take it off.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinelocallorax
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Registered: 07/27/12
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] [Re: mupetmower]
    #23483295 - 07/27/16 06:36 PM (7 years, 6 months ago)

Wish I had seen this thread sooner. Yes.  It should work. Cpc-600 for the win. This thing is amazing. Comes to pressure within minutes and I can slow release pressure. It's a time saver for sure.  For the record,  it can comfortable hold 14 BRF 4oz jars in a single session


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Offlinechristophersons
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: Shroomsh]
    #25190090 - 05/07/18 05:37 PM (5 years, 8 months ago)

I am going to try an instant pot as well.


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Offlineautomaticjack
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: Shroomsh]
    #25406913 - 08/23/18 08:16 AM (5 years, 5 months ago)

I've used my 8 quart Instant Pot to sterilize brf jars and agar dishes. To compensate for the 10-11 PSI vs the 15 of other PCs, I double the time.  So far it seems to be working.

One question though: The IP isn't big enough to hold a quart jar standing up for a grain jar. Could I sterilize it on its side? Any reason that would be a bad idea?

I'm going to answer my own question here, since I've seen in some of the Presto 23 qt demos that to fit 10 quart jars you have to put the last three in sideways. The 8-quart can barely hold two at a time, and you would probably have to run the IP for three hours or more to compensate for the lower PSI, but I might try it just to test whether it works....


Edited by automaticjack (08/25/18 01:17 PM)


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InvisiblemushboyMDiscord
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: automaticjack]
    #25406925 - 08/23/18 08:24 AM (5 years, 5 months ago)

people do that all the time. :thumbup::thumbup:


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Offlinesophocles
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: mushboy]
    #26081444 - 06/29/19 07:26 PM (4 years, 6 months ago)

Here's the science behind using InstantPots and similar products for sterilization:

"Assessment and verification of commercially available pressure cookers for laboratory sterilization" (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289433/)

If you want an electric, commercially available pressure cooker, Zavor's 8 LUX LCD is one of the better ones to get on the market right now. You can fit 7 half pint jars in it.

-S


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Offlinepuppetmastah
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: Shroomsh]
    #26126176 - 08/07/19 10:05 PM (4 years, 5 months ago)

hello, how long did you wait for the PC to release steam before you opened it?


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OfflineMycogolly
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: puppetmastah]
    #26126345 - 08/08/19 02:09 AM (4 years, 5 months ago)

I use an electric pressure cooker because I have extremely limited space and 15psi pressure cookers aren't easily available where I live. The various cooking programs on an electric cooker were also appealing to me (and they've served me well!)

I've had plenty of success by adding between 20 minutes and an hour of extra time to the pressure cooking time. I have also had contamination, but I'm quite certain that's down to flaws in my sterile technique rather than something that can be entirely pinned on the pressure cooker. I've experimented by leaving jars of pressure cooked grain out for over a week with no contamination appearing.

YMMV. Obviously there are "ideal" ways of doing things in this hobby and using a nice, big 15psi pressure cooker is one of those things. But I've seen lots of people preach that you're pretty much going to fail if you use anything below 15psi and that's not entirely honest. By the time I learnt my pressure cooker "wouldn't work", I was already successfully growing Cambodians from multi-spore inoculated WBS in a shoebox.


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Offlinepuppetmastah
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: Mycogolly]
    #26126391 - 08/08/19 03:52 AM (4 years, 5 months ago)

After you finish PC's your jars, how long did you wait to open the instant pot? they said you aren't supposed to force the steam out, so im wondering how long you waited until the jars were cool enough to be removed.


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Offlinemycodose
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Re: Instant Pot for sterilization? [Re: Supalemonhaze]
    #28120158 - 01/01/23 03:03 PM (1 year, 28 days ago)

Are u fucking stupid?


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InvisibleAyePlusS
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Re: Instant Pot for sterilization? [Re: mycodose] * 1
    #28120235 - 01/01/23 04:10 PM (1 year, 28 days ago)

Quote:

mycodose said:
Are u fucking stupid?





The post youโ€™re replying to is over 6 years old, by a poster who hasnt posted in over 5, in a thread that hasnt been active for 3 years and on top of that you blatantly ignored the warning not to reply unless it was truly relevant, (which it clearly isnโ€™t) and added absolutely nothing to the conversation

Great first post!

:coolpost:


--------------------
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OfflineScrewup
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Re: Instant Pot for sterilization? [Re: AyePlus]
    #28120275 - 01/01/23 04:28 PM (1 year, 28 days ago)

Quote:

AyePlus said:
Quote:

mycodose said:
Are u fucking stupid?





The post youโ€™re replying to is over 6 years old, by a poster who hasnt posted in over 5, in a thread that hasnt been active for 3 years and on top of that you blatantly ignored the warning not to reply unless it was truly relevant, (which it clearly isnโ€™t) and added absolutely nothing to the conversation

Great first post!

:coolpost:



My god I couldnโ€™t have said it better myself


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