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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
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OG Cultivator
Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: automaticjack]
    #25406925 - 08/23/18 08:24 AM (5 years, 5 months ago)

people do that all the time. :thumbup::thumbup:


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Offlinesophocles
sophocles
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Registered: 06/27/19
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Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: mushboy]
    #26081444 - 06/29/19 07:26 PM (4 years, 6 months ago)

Here's the science behind using InstantPots and similar products for sterilization:

"Assessment and verification of commercially available pressure cookers for laboratory sterilization" (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289433/)

If you want an electric, commercially available pressure cooker, Zavor's 8 LUX LCD is one of the better ones to get on the market right now. You can fit 7 half pint jars in it.

-S


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Offlinepuppetmastah
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Registered: 01/07/17
Posts: 51
Last seen: 3 years, 8 months
Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: Shroomsh]
    #26126176 - 08/07/19 10:05 PM (4 years, 5 months ago)

hello, how long did you wait for the PC to release steam before you opened it?


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OfflineMycogolly
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Registered: 05/27/19
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Last seen: 4 years, 3 months
Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: puppetmastah]
    #26126345 - 08/08/19 02:09 AM (4 years, 5 months ago)

I use an electric pressure cooker because I have extremely limited space and 15psi pressure cookers aren't easily available where I live. The various cooking programs on an electric cooker were also appealing to me (and they've served me well!)

I've had plenty of success by adding between 20 minutes and an hour of extra time to the pressure cooking time. I have also had contamination, but I'm quite certain that's down to flaws in my sterile technique rather than something that can be entirely pinned on the pressure cooker. I've experimented by leaving jars of pressure cooked grain out for over a week with no contamination appearing.

YMMV. Obviously there are "ideal" ways of doing things in this hobby and using a nice, big 15psi pressure cooker is one of those things. But I've seen lots of people preach that you're pretty much going to fail if you use anything below 15psi and that's not entirely honest. By the time I learnt my pressure cooker "wouldn't work", I was already successfully growing Cambodians from multi-spore inoculated WBS in a shoebox.


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Offlinepuppetmastah
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Registered: 01/07/17
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Last seen: 3 years, 8 months
Re: Instant Pot for sterilization? [UPDATE: so far so good!] pics [Re: Mycogolly]
    #26126391 - 08/08/19 03:52 AM (4 years, 5 months ago)

After you finish PC's your jars, how long did you wait to open the instant pot? they said you aren't supposed to force the steam out, so im wondering how long you waited until the jars were cool enough to be removed.


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Offlinemycodose
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Registered: 12/26/22
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Last seen: 9 months, 10 days
Re: Instant Pot for sterilization? [Re: Supalemonhaze]
    #28120158 - 01/01/23 03:03 PM (1 year, 28 days ago)

Are u fucking stupid?


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InvisibleAyePlusS
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Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Instant Pot for sterilization? [Re: mycodose] * 1
    #28120235 - 01/01/23 04:10 PM (1 year, 28 days ago)

Quote:

mycodose said:
Are u fucking stupid?





The post youโ€™re replying to is over 6 years old, by a poster who hasnt posted in over 5, in a thread that hasnt been active for 3 years and on top of that you blatantly ignored the warning not to reply unless it was truly relevant, (which it clearly isnโ€™t) and added absolutely nothing to the conversation

Great first post!

:coolpost:


--------------------
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C10โ€™s agar guide
Good surface conditions = Good pinsets
Read more, post less.
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OfflineScrewup
Googles your dumb questions
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Registered: 01/27/22
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Re: Instant Pot for sterilization? [Re: AyePlus]
    #28120275 - 01/01/23 04:28 PM (1 year, 28 days ago)

Quote:

AyePlus said:
Quote:

mycodose said:
Are u fucking stupid?





The post youโ€™re replying to is over 6 years old, by a poster who hasnt posted in over 5, in a thread that hasnt been active for 3 years and on top of that you blatantly ignored the warning not to reply unless it was truly relevant, (which it clearly isnโ€™t) and added absolutely nothing to the conversation

Great first post!

:coolpost:



My god I couldnโ€™t have said it better myself


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