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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Bacteria associated with truffle-fruiting bodies?
#23440169 - 07/14/16 07:58 AM (7 years, 6 months ago) |
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What do you guys think, or if you can find any link about this please post 
Asking because while growing sclerotia, i tend to find a little bacteria here and there, and the mycelium seems very vigorous and fighting it off.
So my question is when the sclerotia develops, would the bacteria survive inside it? Could it be dangerous to ingest, since the byproducts of the bacteria are still inside it, or could it be pushed out? which i highly doubt.
These jars were made by LI (from the same clone), not in the most sterile conditions cus im just testing shit out
I have a couple of pics here:-
 This jar was the most infested with bacteria at first, and you can see how the mycelium is looking pretty viscous and that line goes around the whole jar, its like its repairing itself, looks kinda cool TBH.
And no sclerotia formed in that jar, very interesting... Could it be because the bacteria and mycelium are still competing? ( FYI this is from the same clone as the one below)
 Now this jar had a tad bit less bacteria visible and recovered pretty fast and produced large ass sclerotia.
And we already know that many restaurants already sell truffles that are found in nature and are not cultivatable, which is HIGHLY infested with soil microbes, could they be risky?
I also highly doubt that because i know that nearly all microbes in the enviroment have different types of antibodies to fight off the competing organisms, so could sclerotia that are grown indoors (edible/psychoactive) have antibodies that can fight competitors, especially bacteria?
I know I've not been very clear in some parts, I'm writing this kinda fast, so just poke me with what ever i haven't been clear with 
Any information/input would be  
*Edited the title*
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
Edited by Teemo 6T3 (07/15/16 07:29 PM)
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 22 days, 4 hours
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Re: Bacteria associated with truffle-fruiting bodies? [Re: Teemo 6T3]
#23449625 - 07/17/16 11:23 AM (7 years, 6 months ago) |
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You bacteria comes from dirty Innoculant If at all present. Bacteria is everywhere. If your body couldnlt handle some random mushroom jar bacteria just breathing normal air would kill you.
The premise you are exploring need not be discussed in an advanced mycology forum since the obvious choice is to use agar.
Furthermore scletoria producers even when 100% clean can look like a yeast infected cunt.
Conclusion: Do not grow sclerotic producers without having mastered clean xfers on agar.
Also to answer your acutal "?" "So my question is when the sclerotia develops, would the bacteria survive inside it?"
Depends on the type but possibly and possibly not Survive. It PROBABLY is not dangerous. In a will it kill me if I eat it type of way. You body is most certainly 90% or more capable of eating anything a nasty bacterial jar could shit out.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Bacteria associated with truffle-fruiting bodies? [Re: Groo]
#23449756 - 07/17/16 12:27 PM (7 years, 6 months ago) |
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......
Im no noob, i know what im doing, my culture is clean, and yes i used agar..
But the way i inoculated it was not, because i wanted to see if it can fight what ever contam present, it was an experiment, i didn't really emphasise on that since i wrote the thread fast...
I just wanna know if the mycelium can produce antibodies when its in nature/indoor.
Im not talking about how to grow sclerotia here, or how to work with agar or non of that shit, because i already know how.
And why shouldn't this be in Advanced mycology? The reason i posted was not because of cultivation or contamination reasons.
I posted to know the interaction of bacteria with sclerotia producers. And if some sort of antibodies are produced. . .
And for your information, i wasn't saying that the 'certain' bacteria would be dangerous for consumption, the actual byproducts could be pathogenic, they can even have the ability to affect a large number of cellular processes. I studied Microbiology so i know what i'm talking about. 
And most bacteria ARE pathogenic, especially since they can mutate/go through other processes like F+ conjugation and produce strains that are resistant to many types of antibiotics, making it hard for us to stop it at its tracks when they are in us, especially gram negative bacteria, since they are hidden by their capsules which sort of work like a camouflage making it hard for human antibodies to recognise.
edit: my bad again, i wasn't so clear in most parts of the thread, my brain just wooshes with info i just wanna type down.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
Edited by Teemo 6T3 (07/17/16 12:45 PM)
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Bacteria associated with truffle-fruiting bodies? [Re: Teemo 6T3]
#23449829 - 07/17/16 12:58 PM (7 years, 6 months ago) |
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Gotta mention also that since most truffles especially those expensive ones in high end restaurants aren't cultivable.
It could be due to some sort of bacteria not being present when cultivating them in labs, this could be revolutionary, but much more studies are needed for that.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 22 days, 4 hours
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Re: Bacteria associated with truffle-fruiting bodies? [Re: Teemo 6T3]
#23450993 - 07/17/16 07:54 PM (7 years, 6 months ago) |
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It's Quite simple and you are not up to date on your truffle cultivation techniques. See http://www.truffletree.com/cultivation/ Good job they can fight off the crap you purposely let them fight. This is your experiment? What are you testing can I read your thesis Hypothesis?
If you actually had 1, I would still think you could ask this question in Normal cult and get a zippadedoodah fast excellent response. Advanced mycology, well its`s advanced, what you are testing is counterproductive.
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 22 days, 4 hours
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Re: Bacteria associated with truffle-fruiting bodies? [Re: Groo]
#23451008 - 07/17/16 07:57 PM (7 years, 6 months ago) |
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Nice jars btw even despite the backatack, I'd risk eating it it were mine probably. I am not suggesting you do that by the way.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Bacteria associated with truffle-fruiting bodies? [Re: Groo]
#23451100 - 07/17/16 08:25 PM (7 years, 6 months ago) |
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Our cultivation is done on sterile media. Where most any bacteria would love to grow. We keep it sterile and grow only the target species.
Culinary truffles probably pose zero risk as the bacteria found around them is harmless. But it would be easy to get harmless or harmful bacteria in cultivation and since we don't know what we got infected shit goes in the trash.
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