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Offlinemorel
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Registered: 07/11/16
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Growing Mycelium on Agar
    #23438420 - 07/13/16 05:17 PM (7 years, 7 months ago)

So I just started experimenting with growing mushrooms about a week ago. I inoculated five agar plates with a white morel spore syringe and five with psilocybe cubensis spores. I also did three tissue cultures with oyster mushrooms I found in the woods. I have everything sitting in a small flow hood that I built, but I did have four plates become contaminated with what looked like green mold, which I discarded.

Now since I've never done this before, I'm kind of confused as to what exactly the mycelium should look like. In all the pictures I've seen it seems to be fairly solid, but some of mine looks really fuzzy and I'm worried it might actually be a contaminant.




The two pictures above are the fuzzy morel mycelium.





These two are the cubensis. They're growing more slowly than the morel, and I noticed what look like tiny little lumps or bubbles where they were inoculated.

So what I'm really asking is does everything look okay? Am I actually growing mycelium or is this just mold or bacteria? It seems to be what it should be, but I just want to make sure before I get further invested in the process.

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Offlinetetherface
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Re: Growing Mycelium on Agar [Re: morel]
    #23438471 - 07/13/16 05:32 PM (7 years, 7 months ago)

i dig the morel myc never seen it on agar before but the cube plates look a little suspect to me i dunno maybe yeast? whenever i start from spores on agar i transfer right when i see some decent growth id say read up on sterile tek and make a few transfers if for nothing else itll be good practice......and welcome aboard:nyan:


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InvisibleSpitballJedi
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Re: Growing Mycelium on Agar [Re: morel]
    #23438493 - 07/13/16 05:38 PM (7 years, 7 months ago)

different species have different growth patterns and there is some variation within a particular species.

I don't know anything about morel myc.

The cube myc looks contaminated. I don't see anything that looks clean.


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OfflineGreenRabbit
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Re: Growing Mycelium on Agar [Re: SpitballJedi]
    #23438531 - 07/13/16 05:47 PM (7 years, 7 months ago)

Quote:

SpitballJedi said:
The cube myc looks contaminated. I don't see anything that looks clean.




Bacteria. Bacteria everywhere.

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InvisibleMad Season
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Re: Growing Mycelium on Agar [Re: GreenRabbit]
    #23439596 - 07/13/16 11:49 PM (7 years, 7 months ago)

Ew. Yeah the 2nd pic looks very moldy. Those cube plates too. I see some satellite bacterial colonies too. Looks like your sterile prep needs some work. Also that's some terrible inoculant.


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OfflinePsilocyBen17
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Re: Growing Mycelium on Agar [Re: Mad Season]
    #23440286 - 07/14/16 08:59 AM (7 years, 7 months ago)

I heard morel myc is very tough to grow on agar.....you need to do something to stabilize it first IIRC.


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