Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDoi
Stranger
Registered: 06/03/16
Posts: 27
Last seen: 3 years, 7 months
For anyone who has the time
    #23436573 - 07/12/16 11:49 PM (7 years, 6 months ago)

Hey all,

I've started the cultivation process of p subs using mycelium found from bark under the patch and placing on agar, the strongest myc was then isolated. The same procedure was done again but using a stem from a p sub pin placed on agar.

Here are some pictures of the agar plates with wedges taken out:









I have taken three agar wedges, one for each jar. Whole rye grain was used (100g for each 500ml jar mixed with 100-110g of water. Each jar was fitted with a polyfil filter and pressure cooked for one hour. Jars were taken out and shaken to separate wet from dry grain. Once jars were completely cooled agar wedges placed on-top of rye grain and left still.

Here are some pictures of the jars:





I am posting this as some may find it interesting and I wish to know if I am on track or if any crucial mistakes have been made.

Thanks!


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: For anyone who has the time [Re: Doi]
    #23436735 - 07/13/16 01:00 AM (7 years, 6 months ago)

Not bad, hopefully those grains dry up a bit more over the next few days, they're a bit on the wet side.
And try to pressure cook your grain jars for at least 90 minutes.
You want to kill off as many endospores as possible.


Extras: Filter Print Post Top
OfflineDoi
Stranger
Registered: 06/03/16
Posts: 27
Last seen: 3 years, 7 months
Re: For anyone who has the time [Re: Psilosoulful]
    #23436799 - 07/13/16 01:56 AM (7 years, 6 months ago)

Cheers, I used this guide: http://www.fungifun.org/English/Grain-For-Simple-Minds

Does it matter where the jars are stored while the mycelium builds up? They obviously require gas exchange, but should they be placed in an extremely sterile area? or is it okay to leave them in a drawer or something.


Extras: Filter Print Post Top
InvisibleBunya
Male User Gallery
Registered: 06/04/16
Posts: 601
Re: For anyone who has the time [Re: Doi]
    #23436830 - 07/13/16 02:16 AM (7 years, 6 months ago)

Nice job.


--------------------
Bunya cones kill.


Edited by Bunya (01/04/17 12:12 AM)


Extras: Filter Print Post Top
InvisibleBunya
Male User Gallery
Registered: 06/04/16
Posts: 601
Re: For anyone who has the time [Re: Bunya]
    #23436834 - 07/13/16 02:19 AM (7 years, 6 months ago)

Also have you tried a liquid culture with the agar?


--------------------
Bunya cones kill.


Extras: Filter Print Post Top
OfflineNDStepp84
Stellar nuclear waste
Male User Gallery

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: For anyone who has the time [Re: Doi]
    #23436939 - 07/13/16 04:33 AM (7 years, 6 months ago)

Do you have extremely sterile areas in your house? I know I don't. A shelf or something is fine.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: For anyone who has the time [Re: NDStepp84]
    #23436954 - 07/13/16 04:46 AM (7 years, 6 months ago)

Those grains look so bacterial.  I wouldn't even put agar to them, straight to the trash.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: For anyone who has the time [Re: Inocuole]
    #23436967 - 07/13/16 04:53 AM (7 years, 6 months ago)

Quote:

Inocuole said:
Those grains look so bacterial.  I wouldn't even put agar to them, straight to the trash.




That's just what they look like when you dont cook them first, especially if you use 110% water.


They can't be bacterial if they only came out of the PC a few hours before the photo was taken.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: For anyone who has the time [Re: weetsie]
    #23437044 - 07/13/16 06:08 AM (7 years, 6 months ago)

Quote:

weetsie said:
They can't be bacterial if they only came out of the PC a few hours before the photo was taken.




Wanna bet?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: For anyone who has the time [Re: Inocuole]
    #23437057 - 07/13/16 06:14 AM (7 years, 6 months ago)

:whathesaid:

They may be just hella starchy RN but there are endospores right now just waiting to germinate. Once they do, they'll just rip through the starches. Yeah that shit is going to end up being bacterial af.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineMycologist217
Frank's Disciple
Male User Gallery


Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 11 hours
Trusted Cultivator
Re: For anyone who has the time [Re: Mad Season]
    #23437253 - 07/13/16 08:03 AM (7 years, 6 months ago)

I've been using a 65 minute 15PSI pressure sterilization for my birdseed for probably my last 100 jars, and have not had any issues.


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: For anyone who has the time [Re: Mycologist217]
    #23437262 - 07/13/16 08:07 AM (7 years, 6 months ago)

I think we're talking about how it's sticky and starchy, which is more a prep problem. 65 minutes is a little low though. After changing up to 120 minutes with wbs I was having MUCH less wet grains.

Quote:

Mad Season said:
Oh dear lord no. I'll get a quote from when I had this debate awhile back. There's a big difference between fully colonized and cleanly colonized.
Quote:

Mad Season said:
Well there's a difference between colonized and cleanly colonized.

This is colonized:

Lots of members have noticed cobweb and trich from jars that look like this or better. Wet spots are bacteria. Not wet grains or wet spots. To colonize cleanly is the hardest. I had to change my pc times to 2 hours to reduce it.

This is (ALMOST) cleanly colonized, that's how hard it is to have it completely clean. This isn't even completely clean:


This is completely clean spawn:



Anyone who's done this for awhile will testify this is the hardest part of the hobby and is what will dictate the success or failure of bulk.



All those jars had a mushroomy smell. A miniscule amount of bacteria will get overpowered by the mushroom musk. Especially if it is covering the surface




--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: For anyone who has the time [Re: Inocuole]
    #23437269 - 07/13/16 08:11 AM (7 years, 6 months ago)

Quote:

Inocuole said:
Quote:

weetsie said:
They can't be bacterial if they only came out of the PC a few hours before the photo was taken.




Wanna bet?




OK technically they could be but what I'm saying is they look that way because of how the grain was prepped, there is no viable contam in those photos.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: For anyone who has the time [Re: weetsie]
    #23437275 - 07/13/16 08:13 AM (7 years, 6 months ago)

There are, they'll be waking up to a moist delicious feast soon by the looks of it.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
OfflineMycologist217
Frank's Disciple
Male User Gallery


Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 11 hours
Trusted Cultivator
Re: For anyone who has the time [Re: Inocuole]
    #23437294 - 07/13/16 08:23 AM (7 years, 6 months ago)

Oh yeah....hahaha I was pretty worried when I first started getting that whispy colonization you have there in the completely clean pic, but Inocuole was the one that told me it is indicative of exceptionally clean growth, now it's what I aim for:


Sorry my camera is so snotty...hope you can see the resemblance to your example!


As opposed to this:


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


Edited by Mycologist217 (07/13/16 08:33 AM)


Extras: Filter Print Post Top
OfflineDoi
Stranger
Registered: 06/03/16
Posts: 27
Last seen: 3 years, 7 months
Re: For anyone who has the time [Re: Doi]
    #23439878 - 07/14/16 03:49 AM (7 years, 6 months ago)

So after looking through the comments I decided to do another jar. I brang 200g of water to the boil and placed 100g rye into this. The heat was then turned to a simmer and the rye was stirred occasionally, after 30 minutes there was no water left and the rye started sticking to the bottom, I added maybe another ~30ml of water to it and simmered for another 10 min. After simmering the rye was strained and a little cold water was poured through it to remove starch. The rye was then placed in a jar and pressure cooked for 70 minutes.

This is the result:



It looks a lot cleaner, although still very wet. There is no starch stuck to the sides of the jar and the rye seems to be quite loose / not sticky.


Extras: Filter Print Post Top
OfflinePsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
Re: For anyone who has the time [Re: Doi]
    #23440274 - 07/14/16 08:52 AM (7 years, 6 months ago)

follow a proper grain prep tek. I don't like boiling rye. too many burst grains. For rye I prefer to soak overnight, simmer, drain,  pc.

Oats I don't mind boiling if I forget to soak them the night before. They are so damn tough that they are impossible to fuck up.

And, as I learned from Innocule, they bounce back much faster after a shake or g2g then rye does. I guess this is attributed to the hard hull(s)


--------------------
PsilocyBen's Trade List!


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: For anyone who has the time [Re: PsilocyBen17]
    #23440568 - 07/14/16 11:09 AM (7 years, 6 months ago)

If you're cooking the grain before hand just use plenty of water and stain off the excess once it's done.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
OfflineDoi
Stranger
Registered: 06/03/16
Posts: 27
Last seen: 3 years, 7 months
Re: For anyone who has the time [Re: weetsie]
    #23446093 - 07/16/16 07:23 AM (7 years, 6 months ago)

Should I expect to be seeing any growth in the grain at this point? Agar was put in on 12/7 10PM


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: For anyone who has the time [Re: Doi]
    #23446144 - 07/16/16 07:58 AM (7 years, 6 months ago)

Normally the wedge will fluff up by the next day, within a few days even a slow species should be colonizing the grain surrounding the wedge.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Clean terr w/bad cake. can still use as is? sBUD 1,025 3 07/08/02 12:56 PM
by sBUD
* Cleaning Jars TinMan 784 6 12/13/02 06:23 PM
by ExtravagantDream
* cleaning after first flush? Xochipili 850 5 02/09/02 07:34 PM
by Xochipili
* Endospores? where, what., in rye? patzee 2,576 9 08/30/01 10:35 PM
by Suntzu
* Corn Starch Liquid Mycelium Culture AlienPrimate 8,366 7 09/09/17 11:22 PM
by ComebackKid
* Coco Coir (cleaning grade) *update*
( 1 2 all )
rossissekc 1,545 29 04/13/18 01:48 PM
by rossissekc
* New Tek is complete, What do you think?
( 1 2 3 all )
NewbieShroomer 24,556 47 05/15/23 07:15 PM
by johnukguy
* Endospore problems CaliLove 1,502 9 11/07/03 05:59 PM
by CaliLove

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
681 topic views. 16 members, 223 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 14 queries.