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Leastham
Mycologist



Registered: 06/15/09
Posts: 126
Last seen: 6 years, 3 months
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Re: Trich... Trich... Trich Again... Please Help [Re: Supalemonhaze]
#23432419 - 07/11/16 03:35 PM (7 years, 6 months ago) |
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Quote:
Supalemonhaze said: How did you prep them? Pour or no pour?
i used this Tek(Except i used powdered Agar not whatever that red agar is that he used)
This is what i have done so far
(NOTE: I DID THIS ALL WHILE TRYING TO MOVE AS SLOW AS I COULD SO I DIDNT CREATE THAT MUCH AIR MOVEMENT) 1. picked the best jar that looks the healthiest. 2. Got my SAB followed this proccess
3. turned my mini round upside down on a alcohol soaked paper towel 4. Wiped the mini round down with alcohol towel, popped the lid, put it back down 5. wiped the jar lid down with alcohol. 6. wiped my torch handle and exacto down as well 7. rinsed my gloved hands with alcohol 8. flamed the blade till red hot 9. Went back in the SAB 10. popped the lid on the jar open 11. grabbed my mini round(while keeping it upside down) 12. cut a few millimeters off the side of the jar growing off a Seed 13. flipped my mini round put it right in the middle 14. popped the lid back on. 15. closed the jar. 16. wiped the round off again with alcohol 17. put it in a Pitch Black Area, 72-74 degrees
PLEASE ANSWER THESE FOR ME. i have questions about the mini rounds.. do you keep the paper towel on it? do you keep them in the foil? Should the unused ones be kept in the foil? Also do you have to refrigerate the unused ones? PLEASE ANSWER THESE FOR ME.
and as far as this spore thing i have always had great results till now, i also have always wanted to try agar but never got to it, i am getting into it now. thank you guys for pointing out all the negatives about spores, i will try my hardest with the agar
Edited by Leastham (07/11/16 03:37 PM)
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Trich... Trich... Trich Again... Please Help [Re: Leastham]
#23432446 - 07/11/16 03:47 PM (7 years, 6 months ago) |
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You take the foil and paper towels off in your SAB... I take them off of the ones that I'm not going to use right away also, because sometimes the paper towel and micropore tape are damp... This allows the tape to dry... There is no need to keep blanks in the fridge.
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Q&A US vs. THEM The more I learn, the less I know.
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Leastham
Mycologist



Registered: 06/15/09
Posts: 126
Last seen: 6 years, 3 months
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Re: Trich... Trich... Trich Again... Please Help [Re: LocN9ne]
#23432467 - 07/11/16 03:55 PM (7 years, 6 months ago) |
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Quote:
LocN9ne said: You take the foil and paper towels off in your SAB... I take them off of the ones that I'm not going to use right away also, because sometimes the paper towel and micropore tape are damp... This allows the tape to dry... There is no need to keep blanks in the fridge.
alright well thank you very much. i hope to do one more so i have 2 to go from.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: Trich... Trich... Trich Again... Please Help [Re: Pastywhyte]
#23432594 - 07/11/16 05:05 PM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: Maybe the vendors will love us cause we tell people that when they get a bacterial PE syringe that they can just clean it up on agar instead of making the vendor send a new one 
Lol, I don't think so. I used syringe for 3 drops and still have 11 cc's. I'll probably never use it since I have prints now. Getting people to use agar will not help vendors. Syringes are outdated anyway. Print > agar is much better
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: Trich... Trich... Trich Again... Please Help [Re: GreenRabbit]
#23432636 - 07/11/16 05:22 PM (7 years, 6 months ago) |
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Print to agar is ideal...
I agree...
I think people are thinking that because I made a ms syringe from a print that, that is what I prefer.... isolation via agar is how I use to roll and then G2g. ..I don't like lc's since it's even more finicky then a print to syringe.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Trich... Trich... Trich Again... Please Help [Re: GreenRabbit]
#23432663 - 07/11/16 05:34 PM (7 years, 6 months ago) |
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Quote:
GreenRabbit said:
Quote:
Pastywhyte said: Maybe the vendors will love us cause we tell people that when they get a bacterial PE syringe that they can just clean it up on agar instead of making the vendor send a new one 
Lol, I don't think so. I used syringe for 3 drops and still have 11 cc's. I'll probably never use it since I have prints now. Getting people to use agar will not help vendors. Syringes are outdated anyway. Print > agar is much better
Yeah I had my tongue in my cheek when I said that. However despite the fact that we no longer rely on syringes solely to grow, we still recommend sponsors for new people and I may someday still order a new species or variety from a vendor. Maybe the lack of a lock on the market might end up being a good thing. People will give business to decent vendors and the shady ones might not be able to stay in business long due to people not being reliant on syringes.
Besides the lazy factor and urge to shortcut will never be eradicated. Just pop over to Facebook or reddit for a second and you see for yourself.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
#23432800 - 07/11/16 06:17 PM (7 years, 6 months ago) |
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Quote:
HybridprX said: Print to agar is ideal...
I agree...
I think people are thinking that because I made a ms syringe from a print that, that is what I prefer.... isolation via agar is how I use to roll and then G2g. ..I don't like lc's since it's even more finicky then a print to syringe.
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Q&A US vs. THEM The more I learn, the less I know.
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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
#23432891 - 07/11/16 06:56 PM (7 years, 6 months ago) |
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A LC made from an agar wedge is a lot safer than a MS syringe IMO. Less finicky? I would have dissagree and go with LC, you just goda know your shit before attempting to use LC because there is a fair bit to learn before doing so. It isn't for people who like to cut corners. Not as easy as just mixing up some sterile distilled water and spores. You also goda wait for spores to germinate (or not germinate) and then colonize, rather than having vigorous, live culture to begin with...
Clean LC and slurrys are easy to make too, it just takes good sterile technique, agar work and a little research. I've never even attempted to make a MS syringe before... Any MS syringe I ever used straight to grain always had at least a contam jar or two come out of it, they were from reputable sources...
Not to mention, even if a jar looked clean from MS syring, it could still be hiding contams inside (you never saw clean growth on agar first then how do you know otherwise? Even if you did, there could still be contams lingering somewhere in the rest of it); then you g2g and have a trich hay day, sounds like a blast. MS syringes seem quite pointless to me; unless, like pasty said, you are just looking for new genetics and putting them to agar.

At least if you get a contam jar or LI/LC from a clean looking agar wedge, you can be almost certain it was your sterile procedures fault and not the culture source. Like someone else said, unless the fruits were grown invitro and you are super sterile working, a print or syringe would likely still have at least a degree of contamination present. All prints and sryinges should be considered dirty and applied to agar, before anything else, to sector out clean growth.
Don't get me wrong, some success CAN be had with putting spores right to media and skipping agar; sure, that is why noobs do it. You can also have SOME success shitting out a window, does that mean we do it? No. A toilet works much better, because that is what it is designed for. Noobs have to cut corners because the knowledge or material requirements just aren't there yet. But for someone who has been around the block in this hobby and plans to advance, there is no reason or excuse to stay stuck in the stone ages and only work with spores that I can see, other than stupidity or just plain stubbornness. Get with the times, agar is the gate to advanced mycology.
Edited by TheChief (07/12/16 09:46 AM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Trich... Trich... Trich Again... Please Help [Re: TheChief]
#23433880 - 07/12/16 01:54 AM (7 years, 6 months ago) |
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Meh, I gave LCs a break for the time being. Too damn unpredictable. I've had pretty good results in the past when it was common for me to lose jars regardless of what I used but nowadays, even if I catch a contamed LC early during testing, I get kinda pissed off. Most of the time I contam the jar during inoculations, which sucks even worse because I would have already tested the damn thing and would be expecting results.
I mostly use wedges though, I fucking love them. If I ever do need a jar to colonize ASAP, I'll just mix some LI instead of an LC. Safer and it's just as fast, if not faster in some cases, like when you have the culture on a petri already. I inoculate jars with somewhat of a routine so wedges serves my needs perfectly. The extra colonizing time does not bother you when you are spawning something every week.
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HybridprX
Biodegrader of coir



Registered: 01/29/08 
Posts: 2,588
Loc: Canada
Last seen: 6 years, 4 months
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Re: Trich... Trich... Trich Again... Please Help [Re: Supalemonhaze]
#23434041 - 07/12/16 04:54 AM (7 years, 6 months ago) |
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I've done lc's. ...not my preferred course for mass propagation.... G2g is way safer.....but a lc with a fast growing isolate is the fastest means of mass propagation.
I'd prefer a agar slurry to lc. ...that's probably where liquid cultures evolved from.
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