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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Trich... Trich... Trich Again... Please Help [Re: Leastham]
    #23430711 - 07/10/16 10:30 PM (7 years, 6 months ago)

How did you prep them? Pour or no pour?

With poured agar you need to wait a few days to be able to tell if they are clean or not. No-pours are safer because they are already in the containers when they go into the PC. Pouring is faster and less annoying though.

One thing you could do is leave a couple of plates as controls to see if they contam while using the others in the meantime. That way if you start getting contams, you will know if the plates were already contaminated or if you contaminated them during transfers.


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OfflineHybridprX
Biodegrader of coir
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Re: Trich... Trich... Trich Again... Please Help [Re: Supalemonhaze]
    #23431226 - 07/11/16 04:31 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
There is no such thing as a sterile syringe. Agar is the way to go if you want the best chance of getting clean spawn.




You've been brain washed by Rr....
He went around telling the forums this because he urged perfection by isolation... I can make sterile syringes....so can vendors.... vendors wouldn't offer a guarantee if they weren't clean....

Always make a test jar prior to mass inoculation, or, use agar.... homemade spore syringes by new cultivators is where this misconception comes from.


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OfflineSpiritwithin
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Registered: 05/08/16
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
    #23431258 - 07/11/16 04:58 AM (7 years, 6 months ago)

Quote:

HybridprX said:
You've been brain washed by Rr....
He went around telling the forums this because he urged perfection by isolation... I can make sterile syringes....so can vendors.... vendors wouldn't offer a guarantee if they weren't clean....





Would you care to explain to me, how anyone can ever make a 100% sterile syringe from a print, when obviously the mushroom the print was taken from can obviously never be 100% sterile?
Sure, it can be relatively clean, but sterile? No, imho.


--------------------
Trading Golden teacher prints for Pan. cyan. prints.


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InvisibleMad Season
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Registered: 09/16/12
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Re: Trich... Trich... Trich Again... Please Help [Re: Spiritwithin]
    #23431816 - 07/11/16 11:27 AM (7 years, 6 months ago)

Yeah please do explain this 100% sterile spore process you have from a cap that opened in unsterile conditions. When there can be (low balling this because growing in houses are dirty af) 50,000 particles every square inch. Laboratory sterile conditions is 50000 particles every square meter, so even then it still opens up to conditions with potential contaminations.

I'd love to see this vendor guarantee o.0 because I should be getting a refund on LOTS of syringes.. (luckily I had agar to save the day)



From 3 different vendors too

I swear hybrid has something against RR. Literally every other post he calls him a liar and says shit that is pretty ridiculous.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/11/16 11:53 AM)


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Trich... Trich... Trich Again... Please Help [Re: Mad Season]
    #23431891 - 07/11/16 12:03 PM (7 years, 6 months ago)

Well, it is hybrid we are talking about. This is the "TC" who doesn't even recognize bacterial spawn, of course he thinks that his dirty AF homemade syringes are sterile.

A guarantee that a syringe is sterile is new to me too, maybe I need a pair of glasses because I sure as shit never saw that on any of the vendors' sites.


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OfflineNDStepp84
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Re: Trich... Trich... Trich Again... Please Help [Re: Supalemonhaze]
    #23431995 - 07/11/16 12:42 PM (7 years, 6 months ago)

Hell yes vendor syringes can be dirty, especially penis varietys. Some so bad you can't even clean up


Sure they guarantee, I was sent a new one. Personally I would rather guarantee it's clean myself using a penny's worth of agar instead of taking their word for it wasting my time and resources spawning some BS spawn only to get trich. Plus spores to grain is a terrible waste of spores and money. One drop can do countless grows and agar is cheap AF, a dollars worth will do 100 plates, sounds like a no brainier:shrug:


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Trich... Trich... Trich Again... Please Help [Re: NDStepp84]
    #23432029 - 07/11/16 12:55 PM (7 years, 6 months ago)

Quote:

NDStepp84 said:
sounds like a no brainier:shrug:



It is, unless you can't tell the difference between healthy and bacterial spawn after years of growing, then it's a lost cause to even try and explain what is wrong with spores.


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OfflineHybridprX
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Re: Trich... Trich... Trich Again... Please Help [Re: Supalemonhaze]
    #23432071 - 07/11/16 01:12 PM (7 years, 6 months ago)

I never argued the risks of Ms syringes...

When I make my own. ...I inoculate a test jar prior to mass inoculations....that's common sense.

RR urged agar.... since not only can they test it but also isolate if there is a contaminate. ..

And I don't spawn bacteria infected jars...if it smells like mushrooms...there is no "competing" or otherwise "germinated" bacteria. It is a clean culture otherwise the bulk run would fail.

Anyways....that tub I harvested 654 wet grams from is recovering and producing a 2nd flush....guess the bacteria disappeared :smirk:


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Offlinespacechildo
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX] * 1
    #23432087 - 07/11/16 01:19 PM (7 years, 6 months ago)

I think you confuse the terms isolating strains and cleaning up myc.
spore syringes arent like LCs which are generally either clean or dirty.
all spore syringes are dirty, vendors' too, its just that noobs dont recognize the bacteria in their jars
and once they do they start using agar anyway so it doesnt really matter anymore.

hybrid's taking cheap retaliation shots at RR now since he's not around anymore, not the first time and probably not the last.


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Offlinespacechildo
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
    #23432094 - 07/11/16 01:21 PM (7 years, 6 months ago)

Quote:

HybridprX said:
Anyways....that tub I harvested 654 wet grams from is recovering and producing a 2nd flush....guess the bacteria disappeared :smirk:




uuuh, is this a joke? :rofl:
since everyone said bacteria subs still produce,just way less. and you got 2oz from 6qts of spawn.
we usually say less than 1oz pr qt of spawn means somethings wrong. bacteria didnt disappear it ate your crops.
you could also fruit in proper conditions to help your subs produce better.


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InvisibleMad Season
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
    #23432098 - 07/11/16 01:23 PM (7 years, 6 months ago)

:facepalm:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineHybridprX
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Re: Trich... Trich... Trich Again... Please Help [Re: Mad Season]
    #23432131 - 07/11/16 01:36 PM (7 years, 6 months ago)

I'm not taking cheap shots at Rr. ..

I stated the purpose of agar...

If vendor syringes were all dirty...I'd never have grown any and sent out prints to people who grew from them themselves....

You guys don't know the difference between regular p.cube multispore mycelium and a bacterial growth but that's OK. ..it's kind of hard to tell from a picture I guess.

And I agree that the "4l" of spawn did not yeild well on the first flush....that's why I'm expecting 4oz on the 2nd....normally I get 2.5 per 2l of spawn to coir but I'm not blaming bacteria since I've seen genetics play out this way before....it's not a isolate so b/e cannot apply.


--------------------


Edited by HybridprX (07/11/16 01:39 PM)


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InvisibleMad Season
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
    #23432136 - 07/11/16 01:37 PM (7 years, 6 months ago)

Yep typical. Always everyone else but you who's wrong.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleLocN9ne
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Registered: 04/17/15
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Re: Trich... Trich... Trich Again... Please Help [Re: Mad Season]
    #23432141 - 07/11/16 01:40 PM (7 years, 6 months ago)

Isn't there like a 3 strikes and you are out rule for people giving misinformation on the boards? I think there should be, at least catch a time out from being able to post... Its shitty when shitformation gets passed along in current threads, especially when it is done with such confidence... The ONLY way that you could make a sterile spore syringe would be using an invitro agar pin, and even then it would take pretty good sterile technique...

The fact that mushrooms are fruited in open air completely destroys your entire argument hybrid... Unless of course the air on your planet is sterile.... Yes there are some ways to make pretty Damn clean syringes, and a lot of people do... But they are guaranteed to be bacterial to a certain degree...


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Trich... Trich... Trich Again... Please Help [Re: LocN9ne]
    #23432235 - 07/11/16 02:16 PM (7 years, 6 months ago)

As far as hybrid is concerned, you can only have a lot of bacteria, but never a small amount. That's like saying you are not dirty after not taking a shower for a week just because you use a shitload of perfume.

There is no use in trying to teach this guy common sense, he obviously doesn't care for it so I'm not gonna waste my time. You guys shouldn't either.

I mostly grow MS which is cleaned on agar prior to inoculation and I never get what he calls as "p.cube multispore mycelium", unless I fuck up with my technique or miss bacteria in the petri, of course. But yeah, we are the retarded ones for doing it right and interpeting the signs. It's hilarious that such an "experienced" cultivator can have such a skewed view of cultivation.

BTW, I would love to see you explain how you would be able to smell a type of bacteria that doesn't smell. Most bacteria don't.


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Offlinespacechildo
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Re: Trich... Trich... Trich Again... Please Help [Re: Mad Season]
    #23432252 - 07/11/16 02:26 PM (7 years, 6 months ago)

Quote:

Mad Season said:
Yep typical. Always everyone else but you who's wrong.




yeah there's 145 members including several tc's thats wrong about bacteria really being "just the looks of ms" that makes grains clump together and hinder recolonization, and this 1 dude that gets it :lol:

I'm pretty sure he's trolling tho, keeps repeating "mushrooms grew, cant have been bacteria" still today..


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Trich... Trich... Trich Again... Please Help [Re: spacechildo] * 1
    #23432278 - 07/11/16 02:38 PM (7 years, 6 months ago)

I just found out that hybrid has a very bad case of DSD.

Quote:

NDStepp84 said:

It's a condition that the ones suffering don't even know
Mostly acute affecting new growers, but in some rare sad cases it's chronic.:nonono:




Now I feel bad for making fun of him, it's not nice to make fun of people suffering from a disease.

:thisisterrible:


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OfflineHybridprX
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Re: Trich... Trich... Trich Again... Please Help [Re: Supalemonhaze]
    #23432334 - 07/11/16 02:58 PM (7 years, 6 months ago)

Lol@dsd

The vendors are going to hate you guys when every new cultivator blames them for mold....
Or bacteria...

You can start out with a isolate and still have deformities in the mycelium due to fruiting conditions.... high r.h. ..low f.a.e do cause mycelium to behave differently when applied to bulk... just as when it turns dense and yellow around a bacteria splotch....

B/e need not apply to people wanting to shroom it out....go back to advanced mycology which is where this info is coming from.


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX]
    #23432351 - 07/11/16 03:06 PM (7 years, 6 months ago)

I have never calculated my BE but I still refuse to spawn "ms looking jars" (aka bacterial). Both my shrooms and my yields thank me for it.

Not too long ago pasty posted the difference between aerial mycelium from too much rh/too little fae and mycelium reacting to bacteria. The difference is obvious to those willing to learn.

You seem quite happy with low yields and DSD so I'll leave you to it. Thankfully, there are a lot of people who know how to do it right so your misinformation will always be corrected.

PS. I don't think DSD is funny, it's actually quite sad. The fact that you laugh at having it is sadder.


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InvisiblePastywhyteMDiscord
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Re: Trich... Trich... Trich Again... Please Help [Re: HybridprX] * 1
    #23432358 - 07/11/16 03:09 PM (7 years, 6 months ago)

Maybe the vendors will love us cause we tell people that when they get a bacterial PE syringe that they can just clean it up on agar instead of making the vendor send a new one :shrug:


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