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OfflineColonelAngus
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Registered: 04/11/16
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50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened?
    #23425105 - 07/08/16 10:39 PM (7 years, 7 months ago)

I did the Damion 50/50 tek to perfection, got a gallon of clean, fully colonized rye and mixed it all in the same bucket with the coir/verm, and spread between 2 meticulously cleaned new plastic tubs and covered with foil (tight) and lids (loose). One got trich by day 7, the other looks ok. The one that got Trich I was taking quick peeks under the foil after 4-5 days, and the other looks ok, but I didn't peek until I saw the trich on the 1st one.

Is it the peeking? I always read that if I had good spawn, and proper pasteurization, I shouldn't have to worry, but maybe the trich is too rampant up here.

I did an ozone generator treatment and lots of other clean techniques before spawning...

I think it's time for a better substrate tek, but I need a good simple one to choose. I've got PC, large pots etc.. anyone want to suggest a favorite that has lower risk of contams?

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InvisibleCosmoKramer
The Assman

Registered: 06/22/16
Posts: 555
Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: ColonelAngus]
    #23425140 - 07/08/16 10:50 PM (7 years, 7 months ago)

Are you making trays or monos? Trying to understand why you are using foil as a lid.


--------------------
"Get yourself some vitamin C with rose hips and bioflavonoids."

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OfflineNDStepp84
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Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: CosmoKramer] * 1
    #23425158 - 07/08/16 10:54 PM (7 years, 7 months ago)

Something you probably won't want to hear or believe, but it's most likely your spawn.
Bucket tek'd coir is the most fool proof substrate tek.

And before you say it was all white and smelled like fresh mushrooms that dosen't mean anything. Work on your inoculation methods/ sterile tek, start working with agar if you're not and stop shooting spores onto grain if you are because no syringe is 100% clean.

Up your grain PC time to two hours, check your lid filters.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread

Edited by NDStepp84 (07/08/16 11:02 PM)

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Offlinehampiri
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Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: NDStepp84]
    #23425198 - 07/08/16 11:11 PM (7 years, 7 months ago)

I've never had much luck with Coco Coir/Vermiculite myself due to poor spawn. Right now I'm liking slow-pasturized(8 hours at 55*C) cow poop 50%/Jiffy Seed Starting Mix 40%/Vermiculite 10%. The cow poop brings in a lot of microbiota that compete with contaminants while creating free nitrogen and carbon dioxide for the mushrooms to munch on. I've been able to fruit some fairly heinous spawn with that mix. I have had success with Coco Coir inoculated with Pseudomonas putida(commonly available on biological supply sites) as a contaminant resistant casing layer.

Quote:

NDStepp84 said:
Something you probably won't want to hear or believe, but it's most likely your spawn.
Bucket tek'd coir is the most fool proof substrate tek.       

And before you say it was all white and smelled like fresh mushrooms that dosen't mean anything. Work on your inoculation methods/ sterile tek, start working with agar and stop shooting spores onto grain because no syringe is 100% clean




^Second this. Spores->Agar->Grain->G2G then you could use grandmas old panties as a substrate if you wanted to(as long as they are pasturized).


--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin

Edited by hampiri (07/08/16 11:14 PM)

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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 2 months
Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: hampiri]
    #23425532 - 07/09/16 03:10 AM (7 years, 7 months ago)

Quote:

hampiri said:
then you could use grandmas old panties as a substrate if you wanted to(as long as they are pasturized).



:lolwut:

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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: ColonelAngus]
    #23425663 - 07/09/16 04:51 AM (7 years, 7 months ago)

Quote:

ColonelAngus said:
I did the Damion 50/50 tek to perfection




Or did you?

Quote:

Damion5050 said:

Supplies Needed

1 Brick of Coir
2 Quarts of Verm




:jokerclap:

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OfflineColonelAngus
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Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: CosmoKramer]
    #23426529 - 07/09/16 01:01 PM (7 years, 7 months ago)

Quote:

CosmoKramer said:
Are you making trays or monos? Trying to understand why you are using foil as a lid.




I'm making some small tubs, I think they're about 8 quart size sterlite with lids. Then I fruit them in a martha setup.

Seemed like with the approximately 4 inches of headspace above the substrate, the foil would help hold in Co2 better than just the loose lid.

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OfflineColonelAngus
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Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: hampiri]
    #23426558 - 07/09/16 01:07 PM (7 years, 7 months ago)

Quote:

hampiri said:
I've never had much luck with Coco Coir/Vermiculite myself due to poor spawn. Right now I'm liking slow-pasturized(8 hours at 55*C) cow poop 50%/Jiffy Seed Starting Mix 40%/Vermiculite 10%. The cow poop brings in a lot of microbiota that compete with contaminants while creating free nitrogen and carbon dioxide for the mushrooms to munch on. I've been able to fruit some fairly heinous spawn with that mix. I have had success with Coco Coir inoculated with Pseudomonas putida(commonly available on biological supply sites) as a contaminant resistant casing layer.





Wow.. didn't know that about cow poop. Any old brand will be ok? Just stuff from Lowe's or Depot? Also, what kind of recepticle do you use in your pot? I've got oven bags, food saver bags, ziplock bags?

And is this the right Jiffy Stuff?


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OfflineColonelAngus
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Registered: 04/11/16
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Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: NDStepp84]
    #23426572 - 07/09/16 01:11 PM (7 years, 7 months ago)

Quote:

NDStepp84 said:
Something you probably won't want to hear or believe, but it's most likely your spawn.
Bucket tek'd coir is the most fool proof substrate tek.

And before you say it was all white and smelled like fresh mushrooms that dosen't mean anything. Work on your inoculation methods/ sterile tek, start working with agar if you're not and stop shooting spores onto grain if you are because no syringe is 100% clean.

Up your grain PC time to two hours, check your lid filters.




Yeah, I bought some Agar trays, and plan to try to do a biopsy of a bigger/faster sample to Agar, and at least partially isolate some more rhizomorphic sections a few times in a row.

After that, I was hoping to make a liquid culture that I could replicate and store in the fridge indefinitely.

There's just such a stunning array of techniques on this site, and I'm just trying to find one I can reliably replicate without going bananas.

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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: ColonelAngus]
    #23426994 - 07/09/16 03:50 PM (7 years, 7 months ago)

If you are having problems with molds, you're better off using agar wedges or LI for inoculation. LC is rather advanced to start dabbling this early in the game.

Also, to store cultures indefinitely, use slants not LCs. Thickly poured agar plates can be used to store a culture for a while but for any long period of time, slants are superior.

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InvisibleMushiez
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Registered: 04/28/14
Posts: 1,057
Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: ColonelAngus]
    #23427015 - 07/09/16 04:00 PM (7 years, 7 months ago)

For clean tubs with confidence, need clean spawn with confidence, as well as proper past'd substrate

To get clean spawn, you need agar

spores to grain can be hit and miss depending on sterility of prep

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Offlinehampiri
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Posts: 52
Loc: Essos
Last seen: 7 years, 6 months
Re: 50/50 Coir/Verm - 1 Trich Fail, 1 Ok .. what happened? [Re: ColonelAngus]
    #23427818 - 07/09/16 09:36 PM (7 years, 7 months ago)

Quote:

ColonelAngus said:
Quote:

hampiri said:
I've never had much luck with Coco Coir/Vermiculite myself due to poor spawn. Right now I'm liking slow-pasturized(8 hours at 55*C) cow poop 50%/Jiffy Seed Starting Mix 40%/Vermiculite 10%. The cow poop brings in a lot of microbiota that compete with contaminants while creating free nitrogen and carbon dioxide for the mushrooms to munch on. I've been able to fruit some fairly heinous spawn with that mix. I have had success with Coco Coir inoculated with Pseudomonas putida(commonly available on biological supply sites) as a contaminant resistant casing layer.





Wow.. didn't know that about cow poop. Any old brand will be ok? Just stuff from Lowe's or Depot? Also, what kind of recepticle do you use in your pot? I've got oven bags, food saver bags, ziplock bags?

And is this the right Jiffy Stuff?







Regular old cow poop from the hardware store. I just left the whole bag, as I bought it, in my black car all day since it gets to 55-60*C in the summer heat. I would recommend a slow cooker/oven bag. That's the right Jiffy stuff, it already has lime in it, which is nice, I use it for casings too.


--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin

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