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other one


Registered: 11/06/09
Posts: 164
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How to properly clean contaminated terrarium after contamination
#23425583 - 07/09/16 03:48 AM (7 years, 6 months ago) |
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So I had some grain casings that worked, got contaminated with what I guess is trich after a few flushes as usual.. Cleaned them as best I could with alcohol for another grow, and they all contaminated. My questions are what's the best way to clean a terrarium to make sure is sterile, any tricks, and is it possible the room they were grown in is infected, obviously will clean the hell out of room now too. Thanks in advance.
Edited by other one (07/09/16 03:56 AM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: How to properly clean contaminated terrarium after contamination [Re: other one] 1
#23425656 - 07/09/16 04:44 AM (7 years, 6 months ago) |
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Rinse with water. Fruiting isn't even near being sterile and it has no reason to be. If you are seeing contams before or around your first flush, 9 times out of 10 it's from contaminated spawn.
Check your inoculant and try to improve your sterile tek.
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SloppyJoseph
Non-sporalator



Registered: 12/08/15
Posts: 8,511
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Last seen: 3 years, 7 months
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Re: How to properly clean contaminated terrarium after contamination [Re: Supalemonhaze]
#23425737 - 07/09/16 06:27 AM (7 years, 6 months ago) |
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Yeah when you clean a fruiting chamber that had grown mold, soap and water will do, alcohol is just extra. Once you do that, theres nothing more you can do except light the thing on fire. But if you are experiencing contams you need to look back objectively at how you inoculated your grains. Are you using proper sterile technique? Are you using agar to assure a clean culture?
And... what do you mean by terrarium? Like a.. fishtank?
-------------------- AMU Q&A
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blackout


Registered: 07/16/00
Posts: 5,266
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Re: How to properly clean contaminated terrarium after contamination [Re: SloppyJoseph]
#23425792 - 07/09/16 07:16 AM (7 years, 6 months ago) |
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I wash with dishwashing liquid and then sanitize using a popular homebrew plastic fermenter sanitizing mixture, which is water, bleach (sodium hypochlorite) and vinegar (acetic acid). I do a lot of stuff in the same area so do not want anything risky about.
This has to be mixed carefully as it reacts to give off chlorine gas, you never ever mix the 2 of them neat, one goes into water first. It is meant to be a much more effective sanitizer than bleach alone. I have read bleach will not kill mould spores but this mixture is supposed to.
http://www.eurekalert.org/pub_releases/2006-02/asfm-vik021306.php
Quote:
Adding white vinegar to diluted household bleach greatly increases the disinfecting power of the solution, making it strong enough to kill even bacterial spores. Researchers from MicroChem Lab, Inc. in Euless, Texas, report their findings today at the 2006 ASM Biodefense Research Meeting. Sodium hypochlorite (NaOCl) in the form of laundry bleach is available in most households. The concentrate is about 5.25 to 6 percent NaOCl, and the pH value is about 12. Sodium hypochlorite is stable for many months at this high alkaline pH value.
"Laundry bleach is commonly diluted about 10 to 25-fold with tap water to about 2000 to 5000 parts per million of free available chlorine for use as an environmental surface disinfectant, without regard to the pH value of the diluted bleach. However, the pH value is very important for the antimicrobial effectiveness of bleach," says Norman Miner, a researcher on the study.
At alkaline pH values of about 8.5 or higher, more than 90 percent of the bleach is in the form of the chlorite ion (OCl-), which is relatively ineffective antimicrobially. At acidic pH values of about 6.8 or lower, more than 80 percent of the bleach is in the form of hypochlorite (HOCl). HOCl is about 80 to 200 times more antimicrobial than OCl-.
"Bleach is a much more effective antimicrobial chemical at an acidic pH value than at the alkaline Ph value at which bleach is manufactured and stored. A small amount of household vinegar is sufficient to lower the pH of bleach to an acidic range," says Miner.
Miner and his colleagues compared the ability of alkaline (pH 11) and acidified (pH 6) bleach dilutions to disinfect surfaces contaminated with dried bacterial spores, considered the most resistant to disinfectants of all microbes. The alkaline dilution was practically ineffective, killing all of the spores on only 2.5 percent of the surfaces after 20 minutes. During the same time period the acidified solution killed all of the spores on all of the surfaces.
"Diluted bleach at an alkaline pH is a relatively poor disinfectant, but acidified diluted bleach will virtually kill anything in 10 to 20 minutes," says Miner. "In the event of an emergency involving Bacillus anthracis spores contaminating such environmental surfaces as counter tops, desk and table tops, and floors, for example, virtually every household has a sporicidal sterilant available in the form of diluted, acidified bleach."
Miner recommends first diluting one cup of household bleach in one gallon of water and then adding one cup of white vinegar.
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registdewer
Stranger
Registered: 05/05/16
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Re: How to properly clean contaminated terrarium after contamination [Re: blackout]
#23425806 - 07/09/16 07:26 AM (7 years, 6 months ago) |
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to clean the area use 1 to 10 part - bleach to water. the lowest price cleaning method. always wear a mask and glove as it's highly dangerous for health. but this is unnecessary as grow medium does not need to be steriled out.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: How to properly clean contaminated terrarium after contamination [Re: other one]
#23425842 - 07/09/16 08:01 AM (7 years, 6 months ago) |
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Quote:
other one said: So I had some grain casings
misuse of terminology. a casing layer is a thin uncolonized layer on top of your bulk substrate which aids in moisture retention.
what you meant to say is "So I had some bulk substrates"
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SloppyJoseph
Non-sporalator



Registered: 12/08/15
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Re: How to properly clean contaminated terrarium after contamination [Re: registdewer]
#23425975 - 07/09/16 09:20 AM (7 years, 6 months ago) |
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Quote:
registdewer said: to clean the area use 1 to 10 part - bleach to water. the lowest price cleaning method. always wear a mask and glove as it's highly dangerous for health.
-------------------- AMU Q&A
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other one


Registered: 11/06/09
Posts: 164
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Re: How to properly clean contaminated terrarium after contamination [Re: SloppyJoseph]
#23427568 - 07/09/16 07:41 PM (7 years, 6 months ago) |
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Meant a fruiting chamber like buckets/monotubs... I forget some if the correct terminology been awhile since studied it
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other one


Registered: 11/06/09
Posts: 164
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Re: How to properly clean contaminated terrarium after contamination [Re: blackout]
#23427577 - 07/09/16 07:46 PM (7 years, 6 months ago) |
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Sounds legit.. thanks
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