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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 4 months
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Re: Pasty Agar Tek [Re: guyute22]
#23424136 - 07/08/16 04:29 PM (7 years, 7 months ago) |
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So. Pasty Thanks so much for this awesome agar TEK. I know this is older and not been posted on but k thought a new post may attract some more people to try it
Plus I wanted you to see this article I found on Microwave sterilization for Agar Medium. I thought I was interesting considering it apparently is some scientists or whatever that's done the controlled testing on the theory. So I thought I'd let you read it and idk. Try it. I could see it working fine with Agar. By not the BRF Cakes and WBS or Grain. Just don't see it working well with those. I was thinking tho. microwaves do emit some radioactive waves so that would help sterilization too I guess. Plus the heat.
https://counterculturelabs.org/wiki/Microwaving_agar
I DONT USE A MICROWAVE AND HAVE NOT TRIED THIS UNTIL TONIGHT. THIS IS NOT FOR NEWBIES OR ANYONE TO THINK ITS A BETTER METHOD. THIS IS JUST AN INTERESTING ARTICLE I FOJND AND WANTED TO SHARE WITH Pastywwhyte Which you can delete this after wards if you don't want people trying it with your TEK Is understand. I just found it interesting and I was in the middle of your instructions when I took a brake and found it.
I am Steaming 2 in PC(broken) and I also tried 2 in the Microwave. Just to see.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: Skillzd]
#23424353 - 07/08/16 06:08 PM (7 years, 7 months ago) |
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I have tried a few different microwave methods with no success. While I am sure that it could be made to work with certain media and through loads of trial and error to compensate for different microwave specs, altitude differences, etc but, a PC/sterilizer is just easier for me.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I only use the microwave to melt my agar to pour into containers, feel a lot better just devoting 30 minutes to it and knowing it'll get done in the PC.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pasty Agar Tek [Re: Inocuole]
#23424413 - 07/08/16 06:30 PM (7 years, 7 months ago) |
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If I didn't have a PC I would prefer to steam the agar rather than microwave it.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: Pasty Agar Tek [Re: guyute22]
#23424575 - 07/08/16 07:31 PM (7 years, 7 months ago) |
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The first photo is of a transfer, the second is from a pin. i can see good growth at 12oclock but why would you transfer from 10oclock? Does the first plate look fine to inoculate with or no?
Edited by Thoky.ykoht (07/08/16 07:32 PM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Both look fine to me.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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If the second is from a pin, and you haven't made a transfer yet, I would go ahead and make ate art one for good measure.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 3 months, 18 days
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Re: Pasty Agar Tek [Re: Skillzd]
#23425853 - 07/09/16 08:13 AM (7 years, 7 months ago) |
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Quote:
Skillzd said: Plus I wanted you to see this article I found on Microwave sterilization for Agar Medium. I thought I was interesting considering it apparently is some scientists or whatever that's done the controlled testing on the theory.
Did you actually read what their "controlled testing" was? I have not read them all but most are saying it "worked" if they can see no visual contaminants on the plates after incubating for 24hours. This is not good enough for me. These are likely scientists doing open air inoculations onto petris to determine what contams they are dealing with. If you watch shows like mythbusters you will see them take swabs off toilet seats or whatever and wiping on petris in open air, often without even a mask. The open air contams that do land will take a few more days to start to grow, they just want to see already growing contams multiply. Their plates are ditched soon after.
I microwave agar and LCs all the time, I test any new method by leaving plates/jars for months, not 24hours.
Violet has a no pour agar tek, I do not think it is good enough though, but I have made several posts in that thread saying what I do differently.
https://www.shroomery.org/forums/showflat.php/Number/22707554/fpart/all/vc/1
I talk of microwaving plates inside larger filtered containers. I have not had much success working with small shallow plates as the agar bubbles up and gets into the seal area. Regular size jars work fine. I have square jars too. These can be microwaved upright and while still liquid it can be tilted on its side so agar sets on the side, otherwise its hard to get at the agar in deep jar.

I do not use filters on many jars, I believe a large volume jar should have enough air in it to sustain agar. I am currently trying grains in sealed jars to test this too, jars only partially full of grains.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Pasty Agar Tek [Re: blackout]
#23425857 - 07/09/16 08:17 AM (7 years, 7 months ago) |
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Quote:
blackout said:
Quote:
Skillzd said: Plus I wanted you to see this article I found on Microwave sterilization for Agar Medium. I thought I was interesting considering it apparently is some scientists or whatever that's done the controlled testing on the theory.
Did you actually read what their "controlled testing" was? I have not read them all but most are saying it "worked" if they can see no visual contaminants on the plates after incubating for 24hours. This is not good enough for me. These are likely scientists doing open air inoculations onto petris to determine what contams they are dealing with. If you watch shows like mythbusters you will see them take swabs off toilet seats or whatever and wiping on petris in open air, often without even a mask. The open air contams that do land will take a few more days to start to grow, they just want to see already growing contams multiply. Their plates are ditched soon after.
I microwave agar and LCs all the time, I test any new method by leaving plates/jars for months, not 24hours.
Violet has a no pour agar tek, I do not think it is good enough though, but I have made several posts in that thread saying what I do differently.
https://www.shroomery.org/forums/showflat.php/Number/22707554/fpart/all/vc/1
I talk of microwaving plates inside larger filtered containers. I have not had much success working with small shallow plates as the agar bubbles up and gets into the seal area. Regular size jars work fine. I have square jars too. These can be microwaved upright and while still liquid it can be tilted on its side so agar sets on the side, otherwise its hard to get at the agar in deep jar.

I do not use filters on many jars, I believe a large volume jar should have enough air in it to sustain agar. I am currently trying grains in sealed jars to test this too, jars only partially full of grains.
you microwave metals lids?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Was just gonna ask what he uses for lids. Beat me to the punch. If I were microwaving metal lids I would flip the table over and take cover until it finishes 
Anyone ever put CDs in a microwave? I used to do that when I was a kid
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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I thought it looked good as well. I took a transfer from the second plate on 7/3. Here's both of them today.
The pin plate on the left and a transfer from it on the right. I prepped some rye and small jar of sterile water last night, and was going to try my luck on the pin plate while I wait for the rest to grow out.
Edited by Thoky.ykoht (07/09/16 11:38 AM)
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 3 months, 18 days
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Quote:
Munchauzen said: you microwave metals lids?
Yes, there are many many pages on the subject. The wiki one is OK and has links to the USDA guide to using metal safely in microwaves.
https://en.wikipedia.org/wiki/Microwave_oven#Metal_objects
I also once ran with a scissors, it was in a sealed package in a backpack. but some might still stick with the "rule" they heard without knowing why.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Pasty Agar Tek [Re: blackout]
#23426980 - 07/09/16 03:45 PM (7 years, 7 months ago) |
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Quote:
blackout said:
Quote:
Skillzd said: Plus I wanted you to see this article I found on Microwave sterilization for Agar Medium. I thought I was interesting considering it apparently is some scientists or whatever that's done the controlled testing on the theory.
Did you actually read what their "controlled testing" was? I have not read them all but most are saying it "worked" if they can see no visual contaminants on the plates after incubating for 24hours. This is not good enough for me. These are likely scientists doing open air inoculations onto petris to determine what contams they are dealing with. If you watch shows like mythbusters you will see them take swabs off toilet seats or whatever and wiping on petris in open air, often without even a mask. The open air contams that do land will take a few more days to start to grow, they just want to see already growing contams multiply. Their plates are ditched soon after.
I microwave agar and LCs all the time, I test any new method by leaving plates/jars for months, not 24hours.
Violet has a no pour agar tek, I do not think it is good enough though, but I have made several posts in that thread saying what I do differently.
https://www.shroomery.org/forums/showflat.php/Number/22707554/fpart/all/vc/1
I talk of microwaving plates inside larger filtered containers. I have not had much success working with small shallow plates as the agar bubbles up and gets into the seal area. Regular size jars work fine. I have square jars too. These can be microwaved upright and while still liquid it can be tilted on its side so agar sets on the side, otherwise its hard to get at the agar in deep jar.

I do not use filters on many jars, I believe a large volume jar should have enough air in it to sustain agar. I am currently trying grains in sealed jars to test this too, jars only partially full of grains.
But I just don't get why you even bother with microwaving agar. Why don't you just pc a whole bunch of plates? It seems easier and quicker if you need to sterilize a number of plates and it is a reliable method
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Josex]
#23426988 - 07/09/16 03:48 PM (7 years, 7 months ago) |
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PCing is not a quick process..
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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Re: Pasty Agar Tek [Re: Inocuole]
#23426996 - 07/09/16 03:51 PM (7 years, 7 months ago) |
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Quote:
Inocuole said: PCing is not a quick process..
tell me all about it, you have to both fill water AND tighten the lid on the damn thing.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Pasty Agar Tek [Re: Inocuole]
#23427004 - 07/09/16 03:55 PM (7 years, 7 months ago) |
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I mean, it should take longer to sterilize 8 plates with a MW than with a pc, or at least it should be easier with a pc. With a MW you'd need to pay close attention to not mess up.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pasty Agar Tek [Re: Josex]
#23427018 - 07/09/16 04:02 PM (7 years, 7 months ago) |
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With a couple of plates, I can see why someone would want to use a microwave. But still, PCing is the only way I would ever go. Maybe steaming if I was too much of a cheap ass to buy a PC. Unused plates last a while, even if it took me a month to use a single sleeve, that is nowhere near the time it would take for them to go bad.
Admittedly, I have microwaved peat casings for most of my time in cultivation. My PC could only pasteurize 7 jars at a time so instead of making 2 pasteurization cycles, I just threw that in a microwave for ~4 minutes and it's done.
The drum barrel has made my life exceptionally easier though, fits 20-25 jars in a single layer easily. Pasteurization is a piece of cake now, until it's time to wash the jars that is. That part is annoying.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I've never used a microwave to actually sterilize a plate, if I was going to ever do it, I would only be doing it for like 1-2 at a time for an emergency transfer or whatever.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pasty Agar Tek [Re: Inocuole]
#23427077 - 07/09/16 04:22 PM (7 years, 7 months ago) |
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That's the only time I would consider it being appealing. Or maybe for someone who uses a very low amount of plates a month.
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 4 months
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Re: Pasty Agar Tek [Re: Skillzd]
#23429024 - 07/10/16 11:11 AM (7 years, 7 months ago) |
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Yeah it's not something I do. I just thought that was an interesting article that wherever and whatever micro wave they were using worked better then an autoclave. I was thinking too. Microwaves will never be an accurate way. Like someone I seen mentioned. They don't even cook a burrito evenly. Lol. One microwave may work great. The other won't do squat.
Anyways. I have 4 agar plates I made the other day This is the 3rd day and I have something for sure. I know I know. I need to work on my moisture level. Too much moisture in the agar containers. But I also think I may have also gotten too many SPORES The syringes I got this time have tons Last ones I couldn't see many VISIBLE spore clumps. This time there is so many I can see thousands of them in there. And I put about 2 drops on each plate. But with the moisture already high they spread all over the plate. Is this what it's suppose to look like when it first starts?
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