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OfflineJanuaryWolf
JW
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Registered: 01/04/15
Posts: 63
Loc: North Texas
Last seen: 5 years, 7 months
Can you re-sterilize contaminated substrates?
    #23424106 - 07/08/16 04:11 PM (7 years, 6 months ago)

I'm going to try.

I inoculated these bags at the same time, same spawn, different spawn bags.

It's aged mixed wood chips, cotton seed hulls, and King Stropharia Oat spawn.
Here it is jumping off well.
Here it looks like nothing is happening.
I noticed the spawn bag had contamination after I inoculated. :noway2:

My plan is to open the bag back up, and clothes pin it back down. Then cook it in my sterilizer. This will help to keep the folds pressed so it will be easier to re-seal it. It is pulse sealed I don't know if the bag would bust or not in the sterilizer. Then I'll inoculate it with something else, probably oysters. Thoughts and experiences are appreciated.
Thanks.


Edited by JanuaryWolf (07/08/16 04:12 PM)


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OfflineParesthesia
Stranger
Male


Registered: 07/02/08
Posts: 1,090
Loc: Texas
Last seen: 5 years, 11 days
Re: Can you re-sterilize contaminated substrates? [Re: JanuaryWolf]
    #23424222 - 07/08/16 05:07 PM (7 years, 6 months ago)

I would burn it!  there's spores coming out if it right now contaminating everything, run away!  It could very well have contaminated from a hole in the bag.


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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot


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OfflineGr0wer
always improving
Male


Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX Flag
Last seen: 5 years, 10 months
Trusted Cultivator
Re: Can you re-sterilize contaminated substrates? [Re: Paresthesia]
    #23424288 - 07/08/16 05:35 PM (7 years, 6 months ago)

One issue with re sterilizing contams is they sometimes have toxins. The only time I've ever re sterilized things was my deep agar culture storage jars. After having one species ive mixed it up autoclaved and re inoculated with a new species. I compost any contaminated jars or bags and wear a P-100 respirator when dumping them.


Edited by Gr0wer (07/08/16 05:37 PM)


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Offlineleschampignons
Biochemistry + Mycology
Male User Gallery

Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME Flag
Last seen: 4 days, 10 hours
Re: Can you re-sterilize contaminated substrates? [Re: JanuaryWolf]
    #23424379 - 07/08/16 06:19 PM (7 years, 6 months ago)

People will tell you not to but I've done it a fair bit with wooden dowels and a few other substrates and haven't had a problem - particularly if the species you reinoculate with is agressive, like oysters or something.


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