Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < First | < Back | 222 | 223 | 224 | 225 | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 | 241 | 242 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: Supalemonhaze]
    #23419094 - 07/07/16 02:54 AM (7 years, 7 months ago)

Yeah, don't wanna be like ol' Inoc..


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,343
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #23419287 - 07/07/16 06:22 AM (7 years, 7 months ago)

I haven't changed my tape in over a year.


Extras: Filter Print Post Top
InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #23419357 - 07/07/16 07:02 AM (7 years, 7 months ago)

Y'all a bunch of cheapos. :heart:


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: Supalemonhaze]
    #23419366 - 07/07/16 07:08 AM (7 years, 7 months ago)

Don't fret little guy, the micropore tape will last more than one cycle without changing.  Wouldn't wanna find yourself being... that guy.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,959
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #23419492 - 07/07/16 08:39 AM (7 years, 7 months ago)

I use plain surgical tape and have basically never changed them. The paper towel provides a good cover for it and they don't get fucked up. I've never had satellites or any bacteria in my fresh plates and I usually store the bulk of them for months and months before using them.


Extras: Filter Print Post Top
Offlineattfalt
Stranger
 User Gallery

Registered: 02/15/16
Posts: 11
Last seen: 5 years, 7 months
Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #23419842 - 07/07/16 10:43 AM (7 years, 7 months ago)

This was probably answered before but I gave up browsing by page 35 so I will go ahead and ask: can I use just any type of honey? What I have on hand is that grainy, opaque honey I use for breakfast, can I use that or do I need refined honey? thanks


Extras: Filter Print Post Top
Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 1 month
Re: Pastywhyte's Easy Agar Tek [Re: attfalt]
    #23420100 - 07/07/16 12:30 PM (7 years, 7 months ago)

Quote:

attfalt said:
This was probably answered before but I gave up browsing by page 35 so I will go ahead and ask: can I use just any type of honey? What I have on hand is that grainy, opaque honey I use for breakfast, can I use that or do I need refined honey? thanks



I'm sure that your honey would work just fine, its the simple sugars in it that support the mycleium's growth. However the best honey you could use is local honey from a nearby bee farm or something. Following that, you just want a lighter colored and less refined honey. Less refined=more simple sugars


--------------------
:vaped: Highwalker :bongload:


Extras: Filter Print Post Top
Offlineattfalt
Stranger
 User Gallery

Registered: 02/15/16
Posts: 11
Last seen: 5 years, 7 months
Re: Pastywhyte's Easy Agar Tek [Re: lukehighwalker710]
    #23420494 - 07/07/16 03:10 PM (7 years, 7 months ago)

Thanks, I was thinking unrefined honey is probably better than the clear stuff, thank you for confirming that.


Extras: Filter Print Post Top
Invisiblec10h12n2o
serial dilutor
 User Gallery

Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Pasty Agar Tek [Re: attfalt]
    #23421115 - 07/07/16 06:48 PM (7 years, 7 months ago)

great tek :smile: glad mini rounds were my first actual success with agar and i have been fascinated ever since :smile:

Ive been reading the agar threads from people more experienced trying to figure out something pretty basic that i somehow missed:

When germinating multispore on agar for a strain isolation, do you wait for clear segments to show up before doing the first transfer? or is it a good idea to transfer to a fresh plate with less condensation on the lid?



thanks pastywhyte!


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: c10h12n2o]
    #23421199 - 07/07/16 07:12 PM (7 years, 7 months ago)

Transfer from the spores as soon as you get clean growth, when the colony is about the size of a nickle. You won't see sectors until you do several transfers anyway.


Extras: Filter Print Post Top
InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Pasty Agar Tek [Re: c10h12n2o]
    #23421202 - 07/07/16 07:13 PM (7 years, 7 months ago)

You transfer it a few times if you wanna do isolation. Some don't even begin to isolate after a bunch of transfers so the sectors are more defined. Personally, I started to seperate them once I could make out the sectors but I see now that this uses up agar needlessly. Instead of having a couple of plates I have 10 plates with 4 cultures each, always needing to be transferred.

If you leave them until the sectors are easily defined, you will be saving a lot of agar in the process, not to mention time.


Extras: Filter Print Post Top
Invisiblec10h12n2o
serial dilutor
 User Gallery

Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Pasty Agar Tek [Re: Supalemonhaze]
    #23421264 - 07/07/16 07:32 PM (7 years, 7 months ago)

thanks pastywhyte and supalemonhaze, i really appreciate the advice :smile:

i have a large number so i will go ahead and do transfers with the ones larger than a nickel


Extras: Filter Print Post Top
OfflineJohnwayne888
Stranger
Registered: 05/16/16
Posts: 11
Last seen: 7 years, 1 month
Re: Pasty Agar Tek *DELETED* [Re: c10h12n2o]
    #23421706 - 07/07/16 10:03 PM (7 years, 7 months ago)

Post deleted by Johnwayne888

Reason for deletion: .



Extras: Filter Print Post Top
Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 1 month
Re: Pasty Agar Tek [Re: Johnwayne888]
    #23421765 - 07/07/16 10:26 PM (7 years, 7 months ago)

Quote:

Johnwayne888 said:
Lets say I inoc the first dish, then wipe, flame, wipe and inoc the second dish, isnt the 2nd drop of flamed syringe dead? It says just do a single drop, i feel like if I flamed my needle the spore is dead because it's so hot. No?



First of all, do not wipe after flaming. Alcohol sanitizes, flame sterilizes.

Second, you lightly depress the syringe to allow a little solution to cool the needle and a drop to fall onto the bottom of your SAB. The next drop goes onto your dish. You can also use the hot syringe to melt a divot into the agar to drop your spores into as well.


--------------------
:vaped: Highwalker :bongload:


Extras: Filter Print Post Top
InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Pasty Agar Tek [Re: lukehighwalker710]
    #23422208 - 07/08/16 02:22 AM (7 years, 7 months ago)

Should skip the wipe altogether. I never wipe my tools with iso unless I'm about to begin a session. Flame sterilization is all you will ever need.


Extras: Filter Print Post Top
InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pasty Agar Tek [Re: Supalemonhaze]
    #23422776 - 07/08/16 08:46 AM (7 years, 7 months ago)

Are either of these worthy of spawning.

The first pic is on its 5th transfer.
The second is on its third. I had taken transfered from the second plate a couple days agao.


Extras: Filter Print Post Top
InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Pasty Agar Tek [Re: Thoky.ykoht]
    #23422782 - 07/08/16 08:48 AM (7 years, 7 months ago)

Yeah, those look healthy enough to use as inoculant. Just let them grow out almost completely and inoculate some grains with wedges.


Extras: Filter Print Post Top
InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pasty Agar Tek [Re: Supalemonhaze]
    #23422840 - 07/08/16 09:14 AM (7 years, 7 months ago)

Cool, I've been using the agar recipe from munchauzen's 123 recipe so I could try out the blenderless liquid inoculate tek.


Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: Pasty Agar Tek [Re: Thoky.ykoht]
    #23422872 - 07/08/16 09:21 AM (7 years, 7 months ago)

It rocks, dude. Munch is the man!  That tek is so freaking easy and quick. Gonna be able to see the results soon enough. I'll be home from the beach to check it out in a couple days =]

Thinking about it is getting me all giddy =p baha


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Extras: Filter Print Post Top
Offlineguyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
Re: Pasty Agar Tek [Re: mupetmower]
    #23423092 - 07/08/16 10:34 AM (7 years, 7 months ago)

Quote:

Thoky.ykoht said:
Are either of these worthy of spawning.

The first pic is on its 5th transfer.
The second is on its third. I had taken transfered from the second plate a couple days agao.



Personally I would take a few small wedges from 10oclock to 12oclock and make plates from those, then use those to innoc.  edit - from the second pic


--------------------


Edited by guyute22 (07/08/16 10:36 AM)


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 222 | 223 | 224 | 225 | 226 | 227 | 228 | 229 | 230 | 231 | 232 | 233 | 234 | 235 | 236 | 237 | 238 | 239 | 240 | 241 | 242 | Next > | Last >

Shop: PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Agar Tek Aristar 5,833 12 08/01/03 06:42 PM
by shirley knott
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,750 8 12/13/19 06:16 PM
by mushboy
* Re: Peroxidated Agar Tek sparks 4,561 6 05/08/00 12:43 AM
by Anonymous
* agar tek? the man 2,777 4 12/16/02 10:45 AM
by the man
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,322 38 05/11/01 11:08 AM
by MAIA
* Mycelium-only tek, Please Help! FaminedShaman 2,567 4 05/06/01 06:49 AM
by egghead
* Antibac Agar, DIY! superduper 4,871 13 12/04/02 02:20 PM
by superduper
* agar cultivation - easy! felixhigh 2,498 3 11/28/01 01:16 AM
by BBShrizz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
797,464 topic views. 31 members, 183 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.017 seconds on 16 queries.