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c10h12n2o
serial dilutor


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Timing of First Transfer, Multispore on Agar
#23417436 - 07/06/16 03:50 PM (7 years, 7 months ago) |
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hi everyone, been lurking for many years, i have learned so much from you guys, thanks a bunch to everyone here for all you contribute 
So I have been germinating a few old-ish spore syringes i forgot about (2 yrs). About a week ago i made 6 plates of 4 different varieties of cubes, and I am starting to see some white fuzzy mycelium starting to develop where i streaked the spores 
I am getting ready to do a few more plates, pan trop this time. The first plates are just now starting to show white growth, definitely not showing segments yet, only a small opaque growth beginning.
I think i poured the plates too hot last time, since almost all of them have lots of condensation on them. I stored each plate in its own ziplock and stacked them upside down, but the condensation was pretty immediate. Hasnt caused any problems yet that i can see, other than obstructing vision.
Should I go ahead and do a transfer on a few of the first plates, especially the ones with excess condensation? maybe try to get them onto a clearer plate so i can differentiate segments when it starts to get visible and less risk of contam? or should i wait for them to show segments before transferring?
warm regards
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woodrow
journeyman
Registered: 03/17/03
Posts: 142
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Re: Timing of First Transfer, Multispore on Agar [Re: c10h12n2o]
#23418957 - 07/07/16 12:36 AM (7 years, 7 months ago) |
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I would wait to make sure the white growth is not a contam.
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c10h12n2o
serial dilutor


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Timing of First Transfer, Multispore on Agar [Re: woodrow]
#23420193 - 07/07/16 01:13 PM (7 years, 7 months ago) |
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much obliged for the input, thanks 
I took a couple of pics of one of the plates, everything looks really good to my novice eye, except for the condensation on the lid. These are 3 shots of the same plate:


also, here is a shot of the underside of the same plate. is the darkness ok? i think i used a big blob of spores on this plate that was visible as black even before the growth started, but it really sticks out against the white:

I have lots more with different levels of growth but very similar in appearance. should i go ahead and do a few transfers or wait until it starts to show segments?
warm regards
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Timing of First Transfer, Multispore on Agar [Re: c10h12n2o]
#23425760 - 07/09/16 06:46 AM (7 years, 7 months ago) |
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I would let it grow out a bit or you risk transferring too many strains which take more plates to get down to isolates.
First plate - germinate spores Second plate - transfer desirable sectors Third plate - confirms if you have isolated strains and if so transfer to grain and slants.
Edited by weetsie (07/09/16 10:51 AM)
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c10h12n2o
serial dilutor


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Timing of First Transfer, Multispore on Agar [Re: weetsie]
#23441178 - 07/14/16 03:26 PM (7 years, 7 months ago) |
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much obliged, thanks for the advice weetsie very helpful
UDATE: here is a thread with pics of some of the next series
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