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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Yeah I'm wondering if it really isn't some user error. I doubt a vendor bought syringe/print would be over 2 years old, especially since they go through so many so often. Unless it was some crazy rare variety that they rarely ever sell, which I'm sure it wasn't.
But I guess you never know.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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I hope, I really hope!!
I don't have date of the print, maybe I'm just paranoid....but the are so many factors that I'm going to became crazy!!!
People use spore that are 2 yrs old......the one I bought from sponsor can be no more that 3 month old....I hope
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things does not exist, everything is a process, so we proceed
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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UPDATE I got cobweb in my older plates, just one day that I was out and I can't clean the place with alcool and I have contam GODDAM
GODDAM GODDAM I'm tired to grow only bacteria and contam since May
I never had a myc!!!! NEVER
I'm a beast, I'm giving up all my activities to focus on this fucking spore and agar that are raping my mind
plz HELP
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things does not exist, everything is a process, so we proceed
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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perhaps you're moving too quickly? make slower, more deliberate movements. move only with purpose. just a though.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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One thing I find helps is to stab the agar with the loop rather than streaking the spores on the agar. Stabbing the agar will get the spores stuck in there, sandwiched, which will hydrate them pretty well with a soft agar recipe.
You could also try a warm pour. PC a batch of agar, soft preferably, and wait for it to cool enough for it to start to thicken. Being careful it's not too hot (needs to be only warm) pour that over your spore inoculated plate. What this will do is sandwich the spores between two soft agar recipes and the warmth of the pour will soften the spore walls, aiding in germination.
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Eywa_devotee
Goddess Worshiper


Registered: 10/04/10
Posts: 1,088
Loc: State of Confusion, Arkan...
Last seen: 3 years, 7 months
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I use sterilized flat tipped wooden toothpicks to scrape spores off foil, the little plastic loops are too bendy and slippery to get a decent scraping easily and the metal ones tend to tear the foil. You can steam sterilize toothpicks by wrapping them in foil and placing them in a steaming basket above the water in a pressure cooker, PC at 15 PSI for 15 minutes to sterilize them and store in a sterilized jar. To get the agar going quickly apply a drop of sterilized water to a portion of your spore print, let it set covered with a empty pitri dish for several hours (8 to 12 seems best), then dip your loop in the wet spores and transfer to your agar plate.
Another method that works well is to get a small glass bottle and a miniature funnel and scrape the spores in sterile water to make your own spore juice. You'll get a lot more failed jars with this method, but the ones that don't Oh boy...
-------------------- "Love one another." "To Love is to know me." "Love is the Law, Love under Will." "In Compassion, all sorrows end." Regardless of the Master, the message is the same- Choose love and you shall live, Choose Fear and you shall die. Help bring peace to this Earth: Love one another, and serve others before yourself.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Loops don't tear foil, you're only rubbing it gently, not stabbing the print. Wire loops with metal handles are the best I've ever used. Some vendors send what is called as a lancet with the loop. It's like a very small lance, I found that even better for sandwiching spores between the agar. I've lost the lancet unfortunately and I can't seem to find a new one on ebay.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
Eywa_devotee said: I use sterilized flat tipped wooden toothpicks to scrape spores off foil, the little plastic loops are too bendy and slippery to get a decent scraping easily and the metal ones tend to tear the foil.
that's literally never happened to me before o.0. You must be using some CHEAP AF tinfoil..
Quote:
You can steam sterilize toothpicks by wrapping them in foil and placing them in a steaming basket above the water in a pressure cooker, PC at 15 PSI for 15 minutes to sterilize them and store in a sterilized jar.
You also should be sterilizing your loops too, not just tooth picks, you probably didn't mean to say that, just came off that way. Also dude swabs/q-tips are 100000x better than toothpicks, and can be PC'd too, though they come out of the package sterile.
Quote:
To get the agar going quickly apply a drop of sterilized water to a portion of your spore print, let it set covered with a empty pitri dish for several hours (8 to 12 seems best), then dip your loop in the wet spores and transfer to your agar plate.
Are you being serious right now? You want the print to have water on it for 8-12 hours before transferring? Just scrape with a loop or use a swab.
Quote:
Another method that works well is to get a small glass bottle and a miniature funnel and scrape the spores in sterile water to make your own spore juice. You'll get a lot more failed jars with this method, but the ones that don't Oh boy...
So your advice is to add tons of other unnecessary contam vectors to the print doing unnecessary stuff? Is this like a joke or something?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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I wonder what he means by "oh boy". Are these cultures somehow stronger in the presence of god knows how many contams? 
We get some pretty thin foil over here,even the heavy duty catering stuff is pretty weak compared to the foil some vendors use or what you guys in the US have. I still never really tore a print before, and I've used my lancet for most of my inoculations. A lancet is pretty much a needle. If you know how to use your tools, you'll be just fine.
You PC your loops as well mad? I haven't PCed mine for a while, flaming seems to get the job done well. I do the same with my scalpel, flame it half way up the handle and let it cool in front of my hood while I wipe my plates or jars with iso.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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I PC my loop and scalpel together. It's the only thing going over open media... I don't want to have any satellite contams
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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I used to do that too, now I just wipe them with iso to get rid of any large dust particles and flame them well to kill everything that's left. My loop's wire is quite long and the handle isn't sturdy, I find that flaming about an inch up the handle is enough. My scalpel can handle more abuse though, I flame that bitch halfway up the handle. I have to let it cool before I start working or it will stick to my hand 
Haven't PCed either of them for a while, I need to buy a good loop handle though, the two I have are made from shitty metal.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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UPDATE 2
ok I dropped a drop of sterile water in the plates (just a drop right??)
Now what I have to expect??
if i look inside the plates I don't see lot of different, it looks as watery as was at the beginning....dunno
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things does not exist, everything is a process, so we proceed
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Spores will take days to germinate. Agar is good, but not that good. 
Did you squirt a drop or two on the table before inoculating your agar? After flaming the needle, the spores in the first drop would be burnt and dead so the first drop needs to go on the table so you won't have a bunch of useless water sploshing around in your petri. Second drop will have viable spores, that's the one you want to be inoculating with. Just a heads up in case you didn't know.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Use swabs for your spores to agar and skip all this other nonsense. Then you'll really know.. I don't think loops are good for much except transfers. I find them unwieldy for putting spores to agar. Using a softer agar with swabs has always worked fine for me but loops not so much. More for second and third transfers.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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Quote:
Supalemonhaze said: Spores will take days to germinate. Agar is good, but not that good. 
Did you squirt a drop or two on the table before inoculating your agar? After flaming the needle, the spores in the first drop would be burnt and dead so the first drop needs to go on the table so you won't have a bunch of useless water sploshing around in your petri. Second drop will have viable spores, that's the one you want to be inoculating with. Just a heads up in case you didn't know.
I drop just sterile water over spore that I inoc. with a loop 7 days ago, so my drop was only water the spore were alredy on agar
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things does not exist, everything is a process, so we proceed
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: d(r)ied print??? [Re: Adden]
#23435109 - 07/12/16 02:31 PM (7 years, 6 months ago) |
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You don't have to drop water on the spores, they will germinate just fine on agar. Especially if it is soft agar.
Quote:
Adden said: Use swabs for your spores to agar and skip all this other nonsense. Then you'll really know.. I don't think loops are good for much except transfers. I find them unwieldy for putting spores to agar. Using a softer agar with swabs has always worked fine for me but loops not so much. More for second and third transfers.
To each his own I guess. Personally, I hate swabs, I wouldn't use them at all if PE weren't so damn shy about dropping spores. I find loops awesome and easy to use, I've had this thing called a lancet that came with my loop and it was even better for stabbing the agar. I streak mainly due to impulse, stabbing has always germinated faster for me.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Lol I don't really have a proper loop. Maybe that's why I have no issues putting spores on agar? I actually went to a craft store and bought clay tools locally.
generic clay tools. Mine have metal handles.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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It's not hard to scrape and steak spores from print to agar. I used to use those tools when I had crafts in school Just took me down memory lane.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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UPDATE 3
I got a contam on the elder plate after I drop the water, on the 7 days old one I drop the water but nothing change, again stuck in this phase
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things does not exist, everything is a process, so we proceed
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