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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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MEA vs Making it yourself...
#23418163 - 07/06/16 07:41 PM (7 years, 6 months ago) |
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I decided to go with malt extract agar. Is there any difference between the premixed mea powder and mixing my own malt extract and agar agar?
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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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Maybe different ratios of agar agar to malt extract?
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
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you can easily do that but i find it best to just my pre made mea
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lone_psychonaut
Pawn



Registered: 09/27/12
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Re: MEA vs Making it yourself... [Re: tripdawg420]
#23418203 - 07/06/16 07:57 PM (7 years, 6 months ago) |
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I found some pre made mea at a good price, I just didn't know if there's any differences or advantages/disadvantages
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Well you can't change the ratios with premade, and premade is much more expensive IME. I got a 1.5 kilo bag of malt I bought for $15 that's lasted me 2 years now, and I'd say I go through about 40g of agar ($2-$3) every month or so..
That's roughly $4 every 40-80 plates, which is about $4 for 80g of MEA.
This was all done locally. You can find powdered agar online for so cheap it isn't even funny. 5 oz for less than $20
Edited by Mad Season (07/06/16 08:09 PM)
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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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Re: MEA vs Making it yourself... [Re: Mad Season]
#23418239 - 07/06/16 08:10 PM (7 years, 6 months ago) |
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Is changing the ratios really vital though?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Not vital, but helpful under different circumstances. Cleaning up a bacterial culture, you can lower nutrients to make it so bacteria doesn't grow as fast, meanwhile mycelium will grow much faster and rizmorphic as it stretches for nutrients. I do this a lot with contaminated transfers. Also really helps with distinguishing rizmorphic mycelium from the other crap. High nutes can make the mycelium more tomentose, and it'll blend in with other molds. As seen here:

Having higher nutrients really aids in germinating. Especially with stubborn spores.
IMO adjusting nutrients makes my life much easier.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 17 hours, 42 minutes
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i use about 135 ml to 5 grams mea thats enought for 10 jars
any thing is going to take afew xfers to get goot ropy growth
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lone_psychonaut
Pawn



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Posts: 95
Loc: Somewhere in the depths o...
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Re: MEA vs Making it yourself... [Re: tripdawg420]
#23418346 - 07/06/16 08:49 PM (7 years, 6 months ago) |
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Thanks guys, but one more question...So if I want to lower a bacterial culture by lowering the nutrients, Would adjusting the ratios by making the mea by myself be any different than using less pre made mea per water ratio?
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: MEA vs Making it yourself... [Re: Mad Season]
#23418357 - 07/06/16 08:53 PM (7 years, 6 months ago) |
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Quote:
Mad Season said: Not vital, but helpful under different circumstances. Cleaning up a bacterial culture, you can lower nutrients to make it so bacteria doesn't grow as fast, meanwhile mycelium will grow much faster and rizmorphic as it stretches for nutrients. I do this a lot with contaminated transfers. Also really helps with distinguishing rizmorphic mycelium from the other crap. High nutes can make the mycelium more tomentose, and it'll blend in with other molds.
Having higher nutrients really aids in germinating. Especially with stubborn spores.
Good to know I thought about lowering sugar (honey) content to decrease bacterial vigor, but nutrients you say. Then again I've only used PDA
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Highwalker
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
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Quote:
lone_psychonaut said: Thanks guys, but one more question...So if I want to lower a bacterial culture by lowering the nutrients, Would adjusting the ratios by making the mea by myself be any different than using less pre made mea per water ratio?
Yes, they would be different. They're talking about Malt extract:agar ratio, with premade it will always be same ratio me:a
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Highwalker
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Yes. for post germination, and culture clean up I do 1g malt extract and 2g agar every 100 ml. For germinating I do 2g malt ex and 2g agar.
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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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Re: MEA vs Making it yourself... [Re: Mad Season]
#23418426 - 07/06/16 09:11 PM (7 years, 6 months ago) |
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Exactly what I needed to know, thank you guys!
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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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I do recall reading where someone used 17grams of mea / liter, I believe, for a more liquid agar for a LC or ms and 20g / liter for a more solid agar for mycelium...
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