Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags   Bridgetown Botanicals Bridgetown Botanicals

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
MEA vs Making it yourself...
    #23418163 - 07/06/16 07:41 PM (7 years, 6 months ago)

I decided to go with malt extract agar. Is there any difference between the premixed mea powder and mixing my own malt extract and agar agar?


--------------------
Feed your head...


Extras: Filter Print Post Top
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
Re: MEA vs Making it yourself... [Re: lone_psychonaut]
    #23418172 - 07/06/16 07:46 PM (7 years, 6 months ago)

Maybe different ratios of agar agar to malt extract?


--------------------
Feed your head...


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 17 hours, 42 minutes
Re: MEA vs Making it yourself... [Re: lone_psychonaut]
    #23418177 - 07/06/16 07:49 PM (7 years, 6 months ago)

you can easily do that but i find it best to just my pre made mea :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
Re: MEA vs Making it yourself... [Re: tripdawg420]
    #23418203 - 07/06/16 07:57 PM (7 years, 6 months ago)

I found some pre made mea at a good price, I just didn't know if there's any differences or advantages/disadvantages


--------------------
Feed your head...


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: MEA vs Making it yourself... [Re: lone_psychonaut]
    #23418219 - 07/06/16 08:03 PM (7 years, 6 months ago)

Well you can't change the ratios with premade, and premade is much more expensive IME. I got a 1.5 kilo bag of malt I bought for $15  that's lasted me 2 years now, and I'd say I go through about 40g of agar ($2-$3) every month or so..

That's roughly $4 every 40-80 plates, which is about $4 for 80g of MEA.

This was all done locally. You can find powdered agar online for so cheap it isn't even funny. 5 oz for less than $20


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/06/16 08:09 PM)


Extras: Filter Print Post Top
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
Re: MEA vs Making it yourself... [Re: Mad Season]
    #23418239 - 07/06/16 08:10 PM (7 years, 6 months ago)

Is changing the ratios really vital though?


--------------------
Feed your head...


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: MEA vs Making it yourself... [Re: lone_psychonaut] * 1
    #23418256 - 07/06/16 08:15 PM (7 years, 6 months ago)

Not vital, but helpful under different circumstances. Cleaning up a bacterial culture, you can lower nutrients to make it so bacteria doesn't grow as fast, meanwhile mycelium will grow much faster and rizmorphic as it stretches for nutrients. I do this a lot with contaminated transfers. Also really helps with distinguishing rizmorphic mycelium from the other crap. High nutes can make the mycelium more tomentose, and it'll blend in with other molds. As seen here:



Having higher nutrients really aids in germinating. Especially with stubborn spores.

IMO adjusting nutrients makes my life much easier.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 17 hours, 42 minutes
Re: MEA vs Making it yourself... [Re: lone_psychonaut]
    #23418260 - 07/06/16 08:16 PM (7 years, 6 months ago)

i use about 135 ml to 5 grams mea thats enought for 10 jars 

any thing is going to take afew xfers to get goot ropy growth


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
Re: MEA vs Making it yourself... [Re: tripdawg420]
    #23418346 - 07/06/16 08:49 PM (7 years, 6 months ago)

Thanks guys, but one more question...So if I want to lower a bacterial culture by lowering the nutrients, Would adjusting the ratios by making the mea by myself be any different than using less pre made mea per water ratio?


--------------------
Feed your head...


Extras: Filter Print Post Top
Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
Re: MEA vs Making it yourself... [Re: Mad Season]
    #23418357 - 07/06/16 08:53 PM (7 years, 6 months ago)

Quote:

Mad Season said:
Not vital, but helpful under different circumstances. Cleaning up a bacterial culture, you can lower nutrients to make it so bacteria doesn't grow as fast, meanwhile mycelium will grow much faster and rizmorphic as it stretches for nutrients. I do this a lot with contaminated transfers. Also really helps with distinguishing rizmorphic mycelium from the other crap. High nutes can make the mycelium more tomentose, and it'll blend in with other molds.

Having higher nutrients really aids in germinating. Especially with stubborn spores.




Good to know  :takingnotes: I thought about lowering sugar (honey) content to decrease bacterial vigor, but nutrients you say. Then again I've only used PDA


--------------------
:vaped: Highwalker :bongload:


Extras: Filter Print Post Top
Offlinelukehighwalker710
Cloud 9 Dweller
Male


Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
Re: MEA vs Making it yourself... [Re: lone_psychonaut]
    #23418366 - 07/06/16 08:56 PM (7 years, 6 months ago)

Quote:

lone_psychonaut said:
Thanks guys, but one more question...So if I want to lower a bacterial culture by lowering the nutrients, Would adjusting the ratios by making the mea by myself be any different than using less pre made mea per water ratio?



Yes, they would be different. They're talking about Malt extract:agar ratio, with premade it will always be same ratio me:a


--------------------
:vaped: Highwalker :bongload:


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: MEA vs Making it yourself... [Re: lukehighwalker710]
    #23418409 - 07/06/16 09:08 PM (7 years, 6 months ago)

Yes. :whathesaid: for post germination, and culture clean up I do 1g malt extract and 2g agar every 100 ml. For germinating I do 2g malt ex and 2g agar.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
Re: MEA vs Making it yourself... [Re: Mad Season]
    #23418426 - 07/06/16 09:11 PM (7 years, 6 months ago)

Exactly what I needed to know, thank you guys!


--------------------
Feed your head...


Extras: Filter Print Post Top
Invisiblelone_psychonaut
Pawn
Male


Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
Re: MEA vs Making it yourself... [Re: lone_psychonaut]
    #23418530 - 07/06/16 09:41 PM (7 years, 6 months ago)

I do recall reading where someone used 17grams of mea / liter, I believe, for a more liquid agar for a LC or ms and 20g / liter for a more solid agar for mycelium...


--------------------
Feed your head...


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags   Bridgetown Botanicals Bridgetown Botanicals


Similar ThreadsPosterViewsRepliesLast post
* sterilizing MEA in the microwave?
( 1 2 all )
comario 2,705 20 09/26/02 06:51 AM
by comario
* What are the main nutrients that mushrooms grow off? thor_ak 4,023 3 05/06/03 01:48 PM
by mtglu
* adding nutrients Mwhod 2,171 4 06/12/02 04:52 AM
by _Lucid_
* Bacterial Contam in MEA upupup 2,712 7 11/23/02 11:38 AM
by Seuss
* myc growing on MEA ready? (pic) e7c 437 1 07/15/03 11:23 PM
by LiL_KuSsH
* DARK malt no good for MEA? Heruuka 968 2 10/13/02 06:01 PM
by Champion des Champignons
* Another note on adding nutrients to jars... JohnnyRespect 925 2 04/30/03 12:28 AM
by Jon_Doe
* Contam free MEA upupup 1,537 3 12/12/02 12:28 PM
by upupup

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,591 topic views. 21 members, 168 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.01 seconds on 14 queries.