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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 5 hours
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: Psychedel.EXE]
    #23421865 - 07/07/16 11:05 PM (7 years, 6 months ago)

Any suggestions then to something i might be overlooking that causes spores to quickly go dormant? when all my prints were fresh i put each on agar and saw growth quickly, in like a few days or so.

PF tek is pretty basic, and again, besides the extra verm to stop the cakes sticking/gripping so hard in the jar, ive followed what little else is there to do, exactly as it says. I didnt compact my BRF mix, i added water to verm first, i used the same measurements, with proper measuring utensils, pressure cook correctly, but germination rates are inconsistent.

What could be making the spores go dormant, if not the cold?
They are packed away in multiple layers of zip lock bags and eventually in nice clean tupperware.


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Offlinehampiri
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom]
    #23421872 - 07/07/16 11:08 PM (7 years, 6 months ago)

How long has it been since inoculation? I've had it take 20 days in cold weather.


--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin


Edited by hampiri (07/07/16 11:09 PM)


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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 5 hours
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: hampiri]
    #23421890 - 07/07/16 11:16 PM (7 years, 6 months ago)

4-5 weeks


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom] * 1
    #23422001 - 07/08/16 12:20 AM (7 years, 6 months ago)

They're not dormant. They're suffering from operator error,


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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 5 hours
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: bodhisatta]
    #23422221 - 07/08/16 02:31 AM (7 years, 6 months ago)

Quote:

Trusted Cultivator said:
They're not dormant. They're suffering from operator error,




and what might that be?
With jars / syringes theres a few more variables but on agar im just wiping spores on jelly culture so where is the error?

I currently do not know what the problem is/might be.


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Offlinehampiri
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom]
    #23422438 - 07/08/16 05:29 AM (7 years, 6 months ago)

Are you following the recipe: 2 parts vermiculite to 1 part brown rice flour to 1 part water?

Did you put a drop of sex lube in your syringe water before you sterilized it? I have forgotten that when making spore syringes and injected (mostly)water. If you forgot, then you need to shake the holy hell out of the syringes to disperse the spores.

As for using white rice flour; it's probably fine, P. cubensis isn't particularly fastidious. The only difference in brown rice is that it has some vitamins, cellulose, and triglycerides from the bran and germ. Bran and germ are polished off to make white rice.
Mushrooms do not require much in the way of vitamins; they are still in white rice, just in low enough amounts that they don't benefit humans, hence why they "enrich" it by adding B vitamins and iron. Mushrooms do love to munch on cellulose but most any polysaccharide or monosaccharide found in nature seems to do.



--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin


Edited by hampiri (07/08/16 05:54 AM)


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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 5 hours
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: hampiri]
    #23422503 - 07/08/16 06:20 AM (7 years, 6 months ago)

i havent any sex lube, though i did use windex once, quite successfully actually, even after 4 months i think it is now, diluting the now myc-syringe, still worked flawlessly as always, annoyingly given how carelessly i made those 2 syringes compared to now. my current GTs were inoculated with my windex syringes. But they were probably a fluke.
For spore distribution ive heard that a high frequency low amplitude vibrator would do it good, and i have some of those so ill be giving it a try tomorrow on a settled syringe.

Also i only use brown rice flour, i tried white back before i could find a coffee grinder for under $50, since it came pre milled but it didnt work out well, ive used BRF ever since.

Ill just reiterate that other than one time ive never used windex again, with the ammonia toxicity and all, but at the same time everything ive ever grown with the syringes made that way have always done very well opposed to everything else.


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom]
    #23422602 - 07/08/16 07:24 AM (7 years, 6 months ago)

In small quantities ammonia supplies free nitrogen to fungi they actually use ammonia or ammonium hydroxide in some industrial fermentations like for fuel ethanol

Not that I suggest Windex for a syringe at all but perspective


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: bodhisatta]
    #23422700 - 07/08/16 08:11 AM (7 years, 6 months ago)

This is highly unlikely with such fresh spores but it could be that they are bunk/dead. You should buy a syringe from a vendor to see if you have the same problem. There are a number of things that can go wrong during the printing/syringe making proccess which makes even experienced cake cultivators only use vendor syringes.

Making your own clean syringes is not as easy as one would think. Once you start using homemade prints on agar you will easily see why this is. Turning those prints into syringes is an extra step which is even riskier.

So to sum it up, make sure you are following the teks perfectly, no deviations of any kind. Try a vendor syringe from a trusted sponsor to see if your spores/syringe making techinique are to blame. And probably most importantly, always use a SAB even if you are only using syringes on cakes.


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Offlineblackout
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom]
    #23423498 - 07/08/16 12:42 PM (7 years, 6 months ago)

Quote:

DrMushroom said:
Any suggestions then to something i might be overlooking that causes spores to quickly go dormant? when all my prints were fresh i put each on agar and saw growth quickly, in like a few days or so.



So are your spores still not growing on agar?

Don't know if you saw my previous post, I think it's blatantly obvious what is happening here. It'd be like complaining your LC is not working and then telling people its made of pure distilled water.


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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 5 hours
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: blackout]
    #23424340 - 07/08/16 06:03 PM (7 years, 6 months ago)

Australia has no syringe vendors. Getting prints is hard enough as it is.

Also they do eventually grow, they just take ages to start. some jars took almost a month, on agar the spores wiped on there with a loop.

its all clean so i dont know what this "operator error" could be, besides not buffering against temperatures. theres no condensation in there side the bag the spore prints bag is inside has a few desicant satchels. And its ALL prints, both mine and others ive gotten from other members.

But, as i mentioned before, thats why ill focus on just 1-2 strains and start using live innoculents from now on. My windex syringes are basically live and they grow really fast and well so if thats any indication of the efficacy, its certainly worth trying. Proper honey water i mean, not windex as a surfacectant.


--------------------

Mushroom Madness for my face and the tree on the moon dinosaur hello yellow im game.

Shaman Australis Botanicals and Mycology (ethnobotany and mycology, an australia based discord) https://discord.gg/UYaH6QT


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Offlineblackout
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: blackout]
    #23424456 - 07/08/16 06:47 PM (7 years, 6 months ago)

Once again

Quote:

blackout said:
Don't know if you saw my previous post, I think it's blatantly obvious what is happening here.



Have you read my first post?

I am also not clear if you answered this
Quote:

blackout said:
So are your spores still not growing on agar?




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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom]
    #23424489 - 07/08/16 06:59 PM (7 years, 6 months ago)

Quote:

DrMushroom said:
its all clean so i dont know what this "operator error" could be.




This is false. There isn't a vendor print/syringe which is totally clean, let alone a homemade print. Not saying that you can't grow with your own spores but the risk is not worth the effort when you can just start with agar and have a guaranteed live and clean culture.

desiccant packs with spore prints seems like a bad idea to me. Dehydrated spores will take infinitely longer to germinate. Not sure if it can actually dehydrate them, never tried it myself but I'm 200% sure that it is unneccessary and can only make things worse. It's the same reason why old spores take longer to germinate.


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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 5 hours
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: Supalemonhaze]
    #23424884 - 07/08/16 09:33 PM (7 years, 6 months ago)

clean enough*

as i said, the dessicants are in the bag outside the bag, the humidity here is usually quite high so condensation would likely appear inside a sealed bag.

They also do grow on agar but some took over a week before anything visible appeared. I have a thick smearing of creeper from my last grow that only now is showing a white spec, not sure yet though if its creeper myc

Also i agree about using spores directly, once i have liquid cultures down ill never use spore water again.


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Offlinehampiri
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Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: DrMushroom]
    #23425148 - 07/08/16 10:52 PM (7 years, 6 months ago)

My recommendation to help eliminate temperature fluctuation as a variable is to get a cheap foam cooler and construct a heat bomb(google "heat bomb tek"). If it does get to 5C in your house at night, I don't envy you, and I could see that slowing things down.


--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: sugar dusting the inside of jars and what about pre-milled rice flour [Re: hampiri]
    #23425558 - 07/09/16 03:30 AM (7 years, 6 months ago)

1 week is pretty average. There is nothing to worry about if it's only taking 1-2 weeks. If that is what annoys you, start messing with agar wedges or LI.

I suggest LI over LC anyday though. Safer.


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