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OfflineTheBurning1
Stranger
Registered: 07/06/16
Posts: 4
Last seen: 7 years, 6 months
Need some input with contam issue
    #23416492 - 07/06/16 10:23 AM (7 years, 6 months ago)

Been trying to grow oysters with the BFR tek and I'm not doing so well. I have read and studied this website for the past year and I follow the steps exactly and every single time I get the mean green growth


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OfflineTheBurning1
Stranger
Registered: 07/06/16
Posts: 4
Last seen: 7 years, 6 months
Re: Need some input with contam issue [Re: TheBurning1]
    #23416507 - 07/06/16 10:25 AM (7 years, 6 months ago)

I follow the 2:1 ratio of fine verm and water and then mix the flour.
I bought a pressure cooker and keep it at 15 psi with the heat turned down to keep the rocker from going crazy (per RR) and I have tape covering the holes with aluminum foil covering the top. I leave it at 15psi for 90 minutes.
I let them sit over night to cool and inoculate in the morning.
I made my own still air box and burner. I rub the box with alcohol and I'm in a closet with no vent and do a deep cleaning with gloves.
I inoculate and then put them in a box and leave them alone for 4 days at a temp range of 76-80 degrees. Every single time (this would be number 5) there is contams. It has to be something I'm doing and I own up to it but I can't figure it out. I run the syringe through the burner between jars and afterwords I wipe the needle with alcohol. One or two times, ok just a bad run it happens from what I read but every single time there is something I'm just not doing right. Any help would be ok (even constructive criticism).
Anyways thanks so much for the help.
Also, product was bought from sponsors but I seriously doubt it's their issue. I have gone through 6 syringes and this has happened on every single one. And I only use 1cc per jar.


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OfflineTheBurning1
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Registered: 07/06/16
Posts: 4
Last seen: 7 years, 6 months
Re: Need some input with contam issue [Re: TheBurning1]
    #23416530 - 07/06/16 10:36 AM (7 years, 6 months ago)

I forgot to mention that I wipe off the top of the jar before I add my dry verm on top as the filter. I do about 1/2 inch of dry verm to top off.


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Need some input with contam issue [Re: TheBurning1]
    #23418747 - 07/06/16 10:51 PM (7 years, 6 months ago)

You wipe the needle with alcohol (a sanitizer) after you've flamed it (sterilized it)? flame kills 100% of contams. alcohol kills 99%. You're adding contams to it by wiping it.

Have you considered you've been getting dirty ass syringes? Agar will tell you if they are dirty, and it'll give you the option to clean it up

Easy as balls agar prep:
https://www.shroomery.org/forums/showflat.php/Number/19208976
Culture cleanup:
https://www.shroomery.org/forums/showflat.php/Number/18430998
Proper techniques:
https://www.shroomery.org/forums/showflat.php/Number/21889950


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineTheBurning1
Stranger
Registered: 07/06/16
Posts: 4
Last seen: 7 years, 6 months
Re: Need some input with contam issue [Re: Mad Season]
    #23419354 - 07/07/16 07:01 AM (7 years, 6 months ago)

I possibly could have received a couple of nasty syringes but not 6. I think I'm pretty sure I have been cleaning the needle before I put in flame just to clean off the excess verm so it won't pop while I put it in the flame...but then again I may have done it without thinking too. I tossed out all the jars (jars themselves) and starting over with brand new jars and new glove box and new syringe and I'll try it again. 
Thank you for that info! I will print it off and study it.


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InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Need some input with contam issue [Re: TheBurning1]
    #23419956 - 07/07/16 11:33 AM (7 years, 6 months ago)

Idk if they're all from the same source, it could very well be dirty syringes. Regardless until you do agar, you'll always be guessing if your inoculant is clean. Most people agree that culture cleanup on agar is the way to do it.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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