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 joekenorer
The Joekenorer
 
Registered: 05/22/07
Posts: 626
Loc: Pensacola, FL.
Last seen: 1 year, 9 months
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 Microwave sterilization. 
#6980791 - 05/29/07 04:27 AM (16 years, 10 months ago) |
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I was wondering about using a microwave to kill bacteria. When our dish sponge gets old and smelly, we microwave it for a few seconds and the smell is gone, apparently because of this thing I read a few months ago in some magazine talking about how microwaving is 99.9 to 100% effective at destroying all bacteria. So what do you think, a reasonable idea to replace pressure cooking and the such?
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My favorites are weeping willows, which aren't really weeping at all. They're very wispy, witty and will dance in the breeze with you. Nothing like a tree that wants to dance with you. Although it doesn't like its thin limbs being pulled at all, it absolutely LOVES it when you walk through them, letting them gently slide over your face and shoulders. If you're naked, the willow considers it to be sex. It will orgasm on your mind and you will blow dream chunks into outer space. All very fun until your neighbor sees you. -The Joekenorer
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Nibin
Getting there
Registered: 11/29/05
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Last seen: 10 years, 11 months
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Re: Microwave sterilization. [Re: joekenorer]
#6980817 - 05/29/07 04:43 AM (16 years, 10 months ago) |
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more than bacteria it's fungus spores you want to kill. Don't know how well it works with those.
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Fahkface
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Registered: 12/11/06
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Loc: In your Mind, Pedro! In y...
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Re: Microwave sterilization. [Re: Nibin]
#6980830 - 05/29/07 04:52 AM (16 years, 10 months ago) |
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Half of my jars are sterilized with a microwave and I don't have ANY problems. Though you should be careful for the verm/brf mixture burns very quickly (stinks awful  ).
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Nibin
Getting there
Registered: 11/29/05
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Re: Microwave sterilization. [Re: Fahkface]
#6980832 - 05/29/07 04:54 AM (16 years, 10 months ago) |
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also, there is the metal issue
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Jaspar
Stranger
Registered: 04/10/07
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Re: Microwave sterilization. [Re: Nibin]
#6980847 - 05/29/07 05:07 AM (16 years, 10 months ago) |
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How long would you have to keep them in the micro oven to have them completely sterilized?
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joekenorer
The Joekenorer
Registered: 05/22/07
Posts: 626
Loc: Pensacola, FL.
Last seen: 1 year, 9 months
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Re: Microwave sterilization. [Re: Jaspar]
#6980851 - 05/29/07 05:15 AM (16 years, 10 months ago) |
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Ok, I've found other threads about microwaving and heres what I've learned. Sterilization occurs anywhere from 5 to 10 minutes on HIGH. If sterilizing a substrate, be sure theres enough moisture that it doesn't dry and burn. Vermiculite heats very quickly and will burn whatever might be touching it. DO NOT USE METAL OF ANY KIND! One thread suggested the use of plastics or "tupper-ware" because you can sterilize the entire thing, including the lid.
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My favorites are weeping willows, which aren't really weeping at all. They're very wispy, witty and will dance in the breeze with you. Nothing like a tree that wants to dance with you. Although it doesn't like its thin limbs being pulled at all, it absolutely LOVES it when you walk through them, letting them gently slide over your face and shoulders. If you're naked, the willow considers it to be sex. It will orgasm on your mind and you will blow dream chunks into outer space. All very fun until your neighbor sees you. -The Joekenorer
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Fahkface
Over-Fiend
Registered: 12/11/06
Posts: 4,821
Loc: In your Mind, Pedro! In y...
Last seen: 13 years, 6 months
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Re: Microwave sterilization. [Re: joekenorer]
#6980963 - 05/29/07 07:01 AM (16 years, 10 months ago) |
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Well, you could try to heat it for 5-10 min. but it's almost certain that the whole stuff will burn. When you use a 800 watt microwave on full power you burn it as soon as you let it in there for more then 3 minutes.
I normally do it for about 2 minutes and have very little problems with contams.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Microwave sterilization. [Re: Fahkface]
#6980968 - 05/29/07 07:04 AM (16 years, 10 months ago) |
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Don't try it with grains. Brown rice doesn't harbor bacterial endospores, which is why simple boiling or steaming works. To ensure you don't burn or screw up your moisture content, simply use a pot of water and steam them.
Grain need to be pressure cooked.
RR
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figurine
Cat Tamer
Registered: 05/24/07
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Re: Microwave sterilization. [Re: joekenorer]
#6981170 - 05/29/07 08:58 AM (16 years, 10 months ago) |
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I think I read in Hints from Heloise or somewhere that microwaving metal is not a problem if the metal is covered with something opaque and microwavable, but it's not something that I want to test in my own microwave.
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"It takes one to know one." -Mel Gibson
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MYSTIQUE
Say Hi to the elves for me.
Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 6 months
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Re: Microwave sterilization. [Re: figurine]
#6981190 - 05/29/07 09:08 AM (16 years, 10 months ago) |
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somewhere around here someone put up a study some doctors or grad students or something did I forget but it said after 3 min all mold was dead on agar and after 7 min on high all bacteria was dead (I might have that backwards?) But after 7 min on high it should be sterilized if you have a clean microwave.
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
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Re: Microwave sterilization. [Re: MYSTIQUE]
#6981366 - 05/29/07 10:00 AM (16 years, 10 months ago) |
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Yeah, the reason verm burns everything in a microwave is that it's got a high metallic content. It's mica. Mica's chock full of metals. You can't really microwave rocks for the same reason... or I would assume so, I guess that's a silly comparison since most people don't have a reason to microwave rocks.
I mean, I have nuked stuff with verm in it without starting a fire, but if there's no cap on it your verm will explode repeatedly and make a big fat mess while getting very, very hot.
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blackout
Registered: 07/16/00
Posts: 5,266
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Re: Microwave sterilization. [Re: joekenorer]
#7166965 - 07/12/07 04:42 PM (16 years, 9 months ago) |
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Quote:
joekenorer said:
Ok, I've found other threads about microwaving and heres what I've learned.
Unfortunately a lot of info in threads in microwaving is bullshit.
Quote:
joekenorer said:Sterilization occurs anywhere from 5 to 10 minutes on HIGH.
Some substrates will not even be anywhere near boiling after 10mins, let alone sterile. That is a common misconception in microwaving. Size does matter with microwaves.
Quote:
joekenorer said:If sterilizing a substrate, be sure theres enough moisture that it doesn't dry and burn.
You should weigh and calculate your moisture content. One mans jar at 15mins on high is not the same as the next guys. The most important tool when microwaving is a weigh scale and some basic maths.
Quote:
joekenorer said: Vermiculite heats very quickly and will burn whatever might be touching it.
If there is no moisture in there it will overheat quickly, also water easily evaporates off it, it wicks it up and boils it off easily. It can get to EXTREME temperatures. I microwaved carbon before and it cracked the pyrex turntable dish inside, then burnt the counter it was lying on. Some materials can get hot enough to melt metal in a microwave. A jar which expands evenly could easily get up to 500C in a microwave, and cause severe burns.
Quote:
joekenorer said:DO NOT USE METAL OF ANY KIND!
Ah my favourite myth of all, often said by people who have metal walls and turntables inside their microwaves! ignorance is bliss, but dangerous...
Search my username if you want tips on microwaving.
And yes, a PC is better!
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Canon
Stranger
Registered: 06/21/16
Posts: 377
Loc: Europe
Last seen: 2 years, 6 months
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Re: Microwave sterilization. [Re: Fahkface]
#23415943 - 07/06/16 03:46 AM (7 years, 9 months ago) |
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Quote:
Fahkface said:
Half of my jars are sterilized with a microwave and I don't have ANY problems. Though you should be careful for the verm/brf mixture burns very quickly (stinks awful ).
Hi!
I tried it and noticed after injecting the spores that the rice burned. Do you think it will however work?
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mindbentempire
Registered: 10/26/09
Posts: 258
Loc: Australia 
Last seen: 3 months, 1 day
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Re: Microwave sterilization. [Re: Canon]
#23416101 - 07/06/16 06:34 AM (7 years, 9 months ago) |
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Hey dude,
Have you noticed the age of the thread?
Regarding your question, you should probably start over if you have another spore print/syringe, because the moisture content of your jars is most likely stuffed.
If you don't, might as well ride it out and see what happens.
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Canon
Stranger
Registered: 06/21/16
Posts: 377
Loc: Europe
Last seen: 2 years, 6 months
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Okay, I am going to wait and see what happens.
Have you ever tried microwave?
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mindbentempire
Registered: 10/26/09
Posts: 258
Loc: Australia
Last seen: 3 months, 1 day
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Re: Microwave sterilization. [Re: Canon]
#23416114 - 07/06/16 06:40 AM (7 years, 9 months ago) |
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I have not used microwave for BRF jars, because you can just steam them for 90 minutes and youre done.
I have however used microwave for agar preparation with barely any contamination issues.
I will be trying grain microwaving as soon as I find plastic microwavable lids.
Or not, if I get a good pressure cooker.
Here is a good read
https://www.shroomery.org/forums/showflat.php/Number/22707554#22707554
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