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OfflineBig Worm
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Agar Pins
    #23414534 - 07/05/16 05:44 PM (7 years, 6 months ago)

So I found a surprise today as I was cleaning up my room.  Their was a jalisco agar plate that i had laying around for a while.  I go to go clean it out and open up the top.






Now, I am the contamination master, I have been trying to get clean spawn for 6 months now and I just cannot do it successfully. My jars always contam.


So apparently, my only way i'll ever produce mushrooms, as little as they are,  is by leaving agar dishes around for a few months lol.




Figured i'd share,  agar pins always interested me.




edit:  for some reason, even though the picture is uploaded, you can't enlarge it, it says I deleted it.  I didn't and even uploaded it twice. 


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Edited by Big Worm (07/05/16 05:46 PM)


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Offlinespacechildo
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Re: Agar Pins [Re: Big Worm]
    #23414569 - 07/05/16 05:54 PM (7 years, 6 months ago)

next time you get an agar pin open the plate in a SAB and put the whole pin right onto a new plate. should come out pretty damn clean,make 1 transfer to new plate and use that to inoc grains.


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Invisibleweetsie
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Re: Agar Pins [Re: Big Worm]
    #23414814 - 07/05/16 07:13 PM (7 years, 6 months ago)

Quote:

Big Worm said:
Now, I am the contamination master, I have been trying to get clean spawn for 6 months now and I just cannot do it successfully. My jars always contam.




I went through a stage where my jars were all contaminating, had me confused for a long time as I never used to have a problem and I had no issue making clean agar plates.

It turned out the problem was when I replaced my cheap low pressure PC with a steam steriliser I wasn't venting it properly so my grain was never sterile in the first place.

Next time you do grain jars leave one unopened as a control to rule out unsterile grain.


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OfflineBig Worm
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Re: Agar Pins [Re: weetsie]
    #23414821 - 07/05/16 07:15 PM (7 years, 6 months ago)

I do use a Sterilizer too.



It builds up pressure though and holds it, I PC oats for 90min, and just turn it off and let the pressure release naturally.



What do you mean "venting"?


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OfflineBig Worm
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Re: Agar Pins [Re: spacechildo]
    #23414825 - 07/05/16 07:17 PM (7 years, 6 months ago)

Quote:

spacechildo said:
next time you get an agar pin open the plate in a SAB and put the whole pin right onto a new plate. should come out pretty damn clean,make 1 transfer to new plate and use that to inoc grains.







I didn't even see it until I opened it up over my sink to dump it.

:facepalm:



When I make more plates, i'll let one sit and hopefully the same happens and I will definitely do that.


:thumbup:


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Invisibleweetsie
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Re: Agar Pins [Re: Big Worm]
    #23414840 - 07/05/16 07:21 PM (7 years, 6 months ago)

you need to let the air be pushed out and replaced by steam as it heats up.

mine has a small hole and once it starts boiling I let it blast steam out for 10 minutes before plugging it with a stick and letting the pressure climb.

also until you fix your problem try 2 hours from the moment you reach 15 psi.


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OfflineBig Worm
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Re: Agar Pins [Re: weetsie]
    #23414848 - 07/05/16 07:24 PM (7 years, 6 months ago)

Oh yes, I do vent the air for at least 10 minutes, usually until steam starts coming out.



I'll try two hours.    I am definitely discouraged after all my failed attempts and am losing interest in the hobby because I just keep failing.  I have learned a lot though.  All my agar transfers come out clean, it's my grain that contams. 

So maybe you are right, I'll PC for longer.


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Invisibleweetsie
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Re: Agar Pins [Re: Big Worm]
    #23414870 - 07/05/16 07:29 PM (7 years, 6 months ago)

10 minutes AFTER steam starts coming out.

I'm pretty sure its overkill, if I'm in the room i just do 5 minutes or so as it's so noisy but until you have clean grainspawn don't take chances, use plenty of safety margin.


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