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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Supalemonhaze]
    #23428975 - 07/10/16 10:49 AM (7 years, 6 months ago)

Well, my trays are still looking good but no fruits yet (9 days so far). I did not give them enough time to fully colonize the bulk. Like I said they look good though, so hopefully i'll see something over the next week. I have definitely had mono tubs that took this long a few times.

I have prepared everything I need to push forward including my SAB. I have some spore syringes as well, but I am actually thinking about trying to clone instead if these trays come through. My plan is to use
Quote:

Supalemonhaze said:
You should put cultures inside slants if you want to keep them for a long time. Master grain jars can be kept for a couple of weeks past 100% colonization, probably even more but it's always best to use them ASAP.

You should try LI rather than GLC, it's safer and definitely cleaner. Grains always have some bacterial endospores present after PCing and by the time the mycelium colonizes the jar, they would have already recovered or close to it.

Even with G2Gs, I always notice signs of bacteria and I PC my grains pretty well.

Wedges would be the best option, now that you have decided to start using agar, you should give those a go. They are a bit slow compared to LI, LC or g2g but they make up for that with clean inoculant. If you start inoculating jars with a routine (say, once a week), waiting for wedges to colonize your grains is not a problem since you always have something going.




Thanks for the input. Do you have a specific LI tek you recommend? I will probably switch to wedges direct to grain next go around, but I need LI or GLC this go around.


Edited by MrPeanutButta (07/10/16 10:55 AM)


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InvisibleSupalemonhaze
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Re: Yeast After G2G [Re: MrPeanutButta] * 1
    #23429932 - 07/10/16 05:42 PM (7 years, 6 months ago)

Blended LI if you can get the stuff you need for it or blenderless if you can't/don't have time for it.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Supalemonhaze]
    #23429950 - 07/10/16 05:53 PM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Blended LI if you can get the stuff you need for it or blenderless if you can't/don't have time for it.




Cool. Yeah I checked both of those teks out earlier. I am not sure which one I will go with yet, but both seem viable. The only issue I see is that I will be using petri dishes that will be larger in diameter than the jar itself, so I could see that being an issue when I want to transfer to the water jar.


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InvisibleSupalemonhaze
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Re: Yeast After G2G [Re: MrPeanutButta] * 1
    #23429964 - 07/10/16 05:59 PM (7 years, 6 months ago)

Not a problem at all, you do have a scalpel, don't you? Just cut the plate in half or quarters and transfer the wedges to the jar, easy peasy.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Supalemonhaze]
    #23430121 - 07/10/16 07:03 PM (7 years, 6 months ago)

Yep I definitely have a scalpel now. Since this thread started I pretty much loaded up on all of the basic equipment I will need. I am actually pretty excited to start working with agar.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23453832 - 07/18/16 06:14 PM (7 years, 6 months ago)

Instead of starting a new thread I wanted to add here and hopefully get some opinions/advice. The two rye/compost trays are moving super slow, but there are a few fruits. It also seems like there is knotting all over the place like pins want to form but they just aren't.

Do I potentially have a contam issue? This is my first time using a tent rather than a monotub and I am worried that it may be dried out as the casing looks a bit dry. Of course you can also see that the myc has grown through the casing but it doesn't look like it's matted/overlay.

Any suggestions are highly appreciated. This grow has been weird for me. I changed all sorts of variables because I wanted to try new things and so far it has just led to uncertainty. Sometimes I also tend to think the worst when nothing is wrong and I just need to be patient.





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InvisiblePastywhyteMDiscord
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23453836 - 07/18/16 06:15 PM (7 years, 6 months ago)

Don't thread jack. It's rude to the OP :nono:


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Pastywhyte]
    #23453861 - 07/18/16 06:22 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Don't thread jack. It's rude to the OP :nono:




I am the OP Pasty haha :tongue:

I probably made it seem like that wasn't the case with the wording I used. I just meant rather than start a new thread asking about my trays I would add it to this thread because it is part of the same grow.


Edited by MrPeanutButta (07/18/16 06:23 PM)


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Re: Yeast After G2G [Re: MrPeanutButta] * 1
    #23453897 - 07/18/16 06:39 PM (7 years, 6 months ago)

:facepalm: :lol:

Sorry bout that. I would say the trays look a smidge dry, but nothing on them suggests contam to me.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Pastywhyte]
    #23453919 - 07/18/16 06:51 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
:facepalm: :lol:

Sorry bout that. I would say the trays look a smidge dry, but nothing on them suggests contam to me.




It's alright haha. Thanks for protecting my thread!

Thanks for your feedback. Would you patch the casing in a situation like this? I am definitely going to avoid over misting to compensate as I know that can cause issues as well.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23456434 - 07/19/16 02:28 PM (7 years, 6 months ago)

I am thinking about applying a light casing layer. I am thinking at this point that the myc has moved super slow through the compost and that it just isn't ready to flush. Seems I should case so that it promotes a more even pinset. Like I said, I can see what seem to be knots, but they are so tiny I can't quite tell. What do you guys think based on the pics?


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InvisibleSupalemonhaze
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Re: Yeast After G2G [Re: MrPeanutButta] * 1
    #23458447 - 07/20/16 04:14 AM (7 years, 6 months ago)

Can't really tell with those pics. Camera is too far away. You already have pins though so you definitely have other knots in there.

I don't like to re-case once I see knots but you could give it a try if you want. I do think it's dry from what I can tell from the pics, the substrate surface needs to have small beads of moisture at all times. Once they look like they are almost gone, you re-mist. Good conditions and evaporation is key for getting the best pinset your culture is capable of.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Supalemonhaze]
    #23459324 - 07/20/16 11:26 AM (7 years, 6 months ago)

I might give it a little longer and increase my misting and fanning. If it continues to move at this rate I may re-case. Thanks Supalemon!


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23466214 - 07/22/16 02:18 PM (7 years, 6 months ago)

Alright, I know it has only been a few days, but all I am seeing are the 4-5 pins maturing right now. Everything else has remained the same. The surface is starting to blue a bit. You guys pointed out that the casing looked dry. I have been misting a few times daily to help with that.

I am getting to a point where I suspect that I allowed the substrate to dry out. I don't know why I was afraid of too much humidity as I have always gotten good results with monotubs that constantly have beads of water on the surface (Lots of fanning of course) and inside of the container.

I'm thinking I might need to dunk and re case. It still looks relatively healthy...just no activity.

Edit: After doing some reading I may just recase rather than dunk. I have dunked monotubs in the past with some success, but I am sure it increases chances for contams.


Edited by MrPeanutButta (07/22/16 02:45 PM)


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InvisibleSupalemonhaze
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23467996 - 07/22/16 11:55 PM (7 years, 6 months ago)

Quote:

MrPeanutButta said:
but I am sure it increases chances for contams.





It does? What about the people who get multiple flushes from their subs, dunking inbetween each flush? Seems to me that clean spawn is what will keep your sub healthy.

Also, fanning is pretty useless, getting your tub dialed in properly is where it's at.


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OfflineMrPeanutButta
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Re: Yeast After G2G [Re: Supalemonhaze]
    #23468042 - 07/23/16 12:18 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Quote:

MrPeanutButta said:
but I am sure it increases chances for contams.





It does? What about the people who get multiple flushes from their subs, dunking inbetween each flush? Seems to me that clean spawn is what will keep your sub healthy.

Also, fanning is pretty useless, getting your tub dialed in properly is where it's at.




I'm basing what I'm saying here off of other threads i've read. As I stated, I have had success with dunking in the past. I just haven't ever dunked before the first flush. That's why I'm wanting to get some opinions...what i'm saying in my post is just conjecture.


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InvisibleSupalemonhaze
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23468084 - 07/23/16 12:42 AM (7 years, 6 months ago)

Dunking will not cause contams, if you get contams before/around/right after your first flush, chances are your spawn was dirty. Dunking before the first flush is probably not going to help you that much if you hydrated the bulk to proper field capacity. Try lifting the tub to see if it's light, if it is, pour a quart or so of water down the side of your sub, even on top will help if you don't have any pins present. After a couple of hours, drain the leftover water that wasn't absorbed.


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InvisibleInocuole
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Re: Yeast After G2G [Re: Supalemonhaze]
    #23468158 - 07/23/16 01:17 AM (7 years, 6 months ago)

Re-casing stuff will cause more contams than dunking..


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Offlinespacechildo
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Re: Yeast After G2G [Re: MrPeanutButta]
    #23468464 - 07/23/16 07:22 AM (7 years, 6 months ago)

Quote:

MrPeanutButta said:
Edit: After doing some reading I may just recase rather than dunk. I have dunked monotubs in the past with some success, but I am sure it increases chances for contams.




WTF have you been reading? got a link?


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InvisibleSupalemonhaze
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Re: Yeast After G2G [Re: spacechildo]
    #23468517 - 07/23/16 07:57 AM (7 years, 6 months ago)

It's this really good tek made ~15 years ago. The OP says that it works like a charm :trollhide:


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