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Offlinesearching
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Lucky MS pinset
    #23410486 - 07/04/16 01:59 PM (7 years, 6 months ago)

I'm pretty happy I got my first awesome looking pin set. The method was spores to wbs, spawned to CV (couldn't find gypsum), and fruited at the time of spawning. Many thanks to all the knowledge on here that I've learned. Inocuole, your oven coir tek among many coir teks, Frank's how to get stuff done, docs wbs tek, etc.

I've only done monotubs a couple times, but this is the best pin set I've had. I guess I got lucky with the MS genetics here. If I do any more in the future maybe I'll try out cloning and agar, but honestly I've had great luck with just a drop of spore solution to wbs. I'm not saying it's the best way, but it seems to work at least 90% of the time.



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OfflineNDStepp84
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Re: Lucky MS pinset [Re: searching]
    #23410491 - 07/04/16 02:02 PM (7 years, 6 months ago)

Fkn aye! Killer :rockon:
Congrats:cheers:


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Offlinehampiri
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Re: Lucky MS pinset [Re: NDStepp84]
    #23410506 - 07/04/16 02:07 PM (7 years, 6 months ago)

Nice!  :letyourspiritsoar:  Do yourself a favor and take some clones from a bunch of different caps. Maybe you'll be able to isolate a particularly aggressive and prolific fruiting strain.


--------------------
"Trust the fungus..." -Mario

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LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin


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Re: Lucky MS pinset [Re: hampiri]
    #23410550 - 07/04/16 02:22 PM (7 years, 6 months ago)

I might clone some but honestly I don't know if I'm going to do another one right now. This tub will last me a long time. If I took a clone and put it on agar how long can I keep it stored and still be usable? Sorry, I'm new to agar but if I do decide to go that route then I'll be doing a lot of reading first.


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Re: Lucky MS pinset [Re: hampiri]
    #23410578 - 07/04/16 02:27 PM (7 years, 6 months ago)

Killer pinset...  congratz!


--------------------
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Offlinehampiri
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Re: Lucky MS pinset [Re: searching]
    #23410623 - 07/04/16 02:38 PM (7 years, 6 months ago)

Quote:

searching said:
I might clone some but honestly I don't know if I'm going to do another one right now. This tub will last me a long time. If I took a clone and put it on agar how long can I keep it stored and still be usable? Sorry, I'm new to agar but if I do decide to go that route then I'll be doing a lot of reading first.




If you take a clone it should last a few months on a clean malt-extract agar plate. Better yet, you could take a culture slant; they will last nearly indefinitely refrigerated.


--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin


Edited by hampiri (07/04/16 02:49 PM)


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Re: Lucky MS pinset [Re: hampiri]
    #23410842 - 07/04/16 03:52 PM (7 years, 6 months ago)

Yea. Make a slant from it. They last for years in the fridge.


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Re: Lucky MS pinset [Re: DeadPhan]
    #23411364 - 07/04/16 06:23 PM (7 years, 6 months ago)

:cookiemonster:Fking beautiful:elmo:


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Re: Lucky MS pinset [Re: searching]
    #23411478 - 07/04/16 06:58 PM (7 years, 6 months ago)

#pinsetGoals
Update with pics please :dancer:


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OfflineMycologist217
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Re: Lucky MS pinset [Re: ComebackKid]
    #23412183 - 07/04/16 10:58 PM (7 years, 6 months ago)

Awesome set dude....excellent work!

But I want to talk about spores for a second....when you say 90% of the time, I can assure you that if you use spores to inoculate birdseed it will be closer to 1/7-1/5 jars you lose plus the syringes that are just outright dirty....spores work....they aren't fool proof..the reason we advocate agar so much over spores is for several reasons the first of which might be we know our inoculant is clean 100% of the time....

Past that working with tissue on agar allows you to preserve genetics...you could have Linsey's like this every time guaranteed...I've used spores for bulk grows several times, but I feel safe assuring you you'll never get the speed, comfort, and consistency you get with agar based inoculants from spores....it's a crap shoot from day one tip end....


Amaxing tub! I encourage you to clone one of those fruits.


--------------------
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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineMycologist217
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Re: Lucky MS pinset [Re: Mycologist217]
    #23412188 - 07/04/16 11:00 PM (7 years, 6 months ago)

I'm doing a clone based grow now that was initiated using agar plates I've had in the fridge since January.

If you want to learn more about what it takes to clone..,,click the cloning guide link in my signature for a full pictorial on how to go from a mushroom to a whole new grow!


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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Re: Lucky MS pinset [Re: Mycologist217]
    #23412275 - 07/04/16 11:31 PM (7 years, 6 months ago)

Quote:

Mycologist217 said:
Awesome set dude....excellent work!

But I want to talk about spores for a second....when you say 90% of the time, I can assure you that if you use spores to inoculate birdseed it will be closer to 1/7-1/5 jars you lose plus the syringes that are just outright dirty....spores work....they aren't fool proof..the reason we advocate agar so much over spores is for several reasons the first of which might be we know our inoculant is clean 100% of the time....

Past that working with tissue on agar allows you to preserve genetics...you could have Linsey's like this every time guaranteed...I've used spores for bulk grows several times, but I feel safe assuring you you'll never get the speed, comfort, and consistency you get with agar based inoculants from spores....it's a crap shoot from day one tip end....


Amaxing tub! I encourage you to clone one of those fruits.




I only say 90% because I've done about 20 jars and haven't had any fail. All of the tubs and trays I've done recently have given me 4-5 flushes before I threw them out with no contams at all. It's easy to calculate the success rate using binomial probability with an online calculator. 20 successes out of 20 tries means there's an 86% success rate with a 90% confidence interval. OK so it's not more than 90% like I said but it's close. This doesn't take into account dirty syringes or poor sterile technique.

I know there are huge benefits to agar though like knowing for sure that you have a clean culture.


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InvisibleTeemo 6T3
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Re: Lucky MS pinset [Re: searching]
    #23412799 - 07/05/16 06:57 AM (7 years, 6 months ago)

:awetongue:


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Re: Lucky MS pinset [Re: Teemo 6T3]
    #23412805 - 07/05/16 06:59 AM (7 years, 6 months ago)

Very very nice pinset,it will rain mushrooms in there hahaha good luck with the fruiting


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OfflineMycoLoopology
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Re: Lucky MS pinset [Re: Mycologist217]
    #23412872 - 07/05/16 07:42 AM (7 years, 6 months ago)

Quote:

Mycologist217 said:
Awesome set dude....excellent work!

But I want to talk about spores for a second....when you say 90% of the time, I can assure you that if you use spores to inoculate birdseed it will be closer to 1/7-1/5 jars you lose plus the syringes that are just outright dirty....spores work....they aren't fool proof..the reason we advocate agar so much over spores is for several reasons the first of which might be we know our inoculant is clean 100% of the time....

Past that working with tissue on agar allows you to preserve genetics...you could have Linsey's like this every time guaranteed...I've used spores for bulk grows several times, but I feel safe assuring you you'll never get the speed, comfort, and consistency you get with agar based inoculants from spores....it's a crap shoot from day one tip end....


Amaxing tub! I encourage you to clone one of those fruits.




i hate MS, and dont advocate for it at all.. and im moving to agar shortly.. HOWEVER, ive had about 90-95% success rate with MS to WBS over the years. maybe my supplier is just good, but i think sterile technique has a LOT to do with it.

even this current grow. while i wasnt too optimistic to start, at the moment, i have 30+ quart jars with amazing mycelial growth; ALL from MS. EVERY single jar i inoculated is doing good so far. we will see as time passes whether or not it stays that way, fingers crossed. but we will cross that bridge when we get there haha.

now i wont say that MS doesnt have bacteria compared to agar. of course it does. but if you work it properly and get growth going quickly, i think you can overcome any bacteria present, quickly enough that it can become negligible at best. it all comes down to being as sterile as humanly possible, and not introducing any of your own contams into the equation.


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Re: Lucky MS pinset [Re: MycoLoopology]
    #23414551 - 07/05/16 05:49 PM (7 years, 6 months ago)



I'm worried that the caps might open early and I'm going to end up with a full tub of mini mushrooms. The big shroom in the corner looks like it could start opening the cap already. Any tips or anything I can do to prevent this? Bottom watering? More fae? I just loosened the polyfil.


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Re: Lucky MS pinset [Re: searching]
    #23414564 - 07/05/16 05:51 PM (7 years, 6 months ago)

I don't know why it's not letting me blow up the picture. It says deleted by the op but I didn't delete it. Here it is again.



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OfflineNDStepp84
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Re: Lucky MS pinset [Re: searching]
    #23414586 - 07/05/16 05:57 PM (7 years, 6 months ago)

You can try a bit of bottom watering and taking out the top poly, but a lot of the times those crazy pinsets will just produce a whole lot of smaller mushrooms. second flush may produce fewer but bigger mushrooms.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Re: Lucky MS pinset [Re: searching]
    #23414695 - 07/05/16 06:34 PM (7 years, 6 months ago)

Try uploading that pic again both say deleted when I try to expand.
Unfortunately I don't think there's much you can do about veils breaking.
I read somewhere that although the mushroom gets bigger from mycelium pumping water in. Too much water can also stunt growth. I think it's just genetics.
I'll try and find that source too if you like it was an interesting read


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Re: Lucky MS pinset [Re: ComebackKid]
    #23414746 - 07/05/16 06:53 PM (7 years, 6 months ago)

Likely takes a ton of resources from the mycelium / substrate to mature that many fruits.  Not sure if the typical response is aborts or small mushies.


Curious about: Spawn ratio, grain type, strain.


Have some tests running with supplemented coir and long consolidation periods.


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OfflineEasyriding123
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Re: Lucky MS pinset [Re: Snazz]
    #23414852 - 07/05/16 07:24 PM (7 years, 6 months ago)

I get smaller mushrooms in my first flush but more. Yours will still grow some more you will be happy. You can partially harvest and let the others catch up a bit. On your next flushes you could expect bigger mushrooms.


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Re: Lucky MS pinset [Re: Easyriding123]
    #23415159 - 07/05/16 09:14 PM (7 years, 6 months ago)

I didn't read the whole thread but IME the biggest issue with getting a dense pinset to fruit well is sufficient supplied water.


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Re: Lucky MS pinset [Re: Snazz]
    #23415702 - 07/06/16 12:53 AM (7 years, 6 months ago)

Quote:

Snazz said:
Likely takes a ton of resources from the mycelium / substrate to mature that many fruits.  Not sure if the typical response is aborts or small mushies.


Curious about: Spawn ratio, grain type, strain.


Have some tests running with supplemented coir and long consolidation periods.




It was 5 quarts of wbs to one brick of coir and like two and a half quarts of verm. The funny thing is that these jars were fully colonized for like two months and had shrunken inside the jar considerably with perfectly clear metabolites filling up the bottom quarter of the jar. I just wasn't ready to use them until my other tub finished up. I wonder if that had anything to do with how things turned out. Maybe the mycelium digested all of the grain and was really ready to fruit.

The strain was golden teacher.


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Offlinehampiri
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Re: Lucky MS pinset [Re: searching]
    #23415713 - 07/06/16 01:05 AM (7 years, 6 months ago)

Figured it was GT :smile: loves those girls.


--------------------
"Trust the fungus..." -Mario

Legend:TakenNever againWant to take
LSD DMT 2C-I Mescaline Bufotenin Psilocin Psilocybin 5-Hydroxypsilocin 5-Hydroxypsilocybin 25I-NBOMe LSZ ETH-LAD DET Ethocin Ethocybin


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Re: Lucky MS pinset [Re: hampiri]
    #23416163 - 07/06/16 07:14 AM (7 years, 6 months ago)

Long consolidation seems to have great benefits.  Jars at 100% still have a ton of food inside the kernels.  Scaling from milo/millet to oats/popcorn being longest


I'm trying one week extra for jar and one week extra in bulk sub (added oil, stevia, vitamins and minerals to coir) and full field capacity (which until now I was hesitant to do)


6 quarts as well.  Hoping to get some weight like you did.  Great job man. :hi5:


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Re: Lucky MS pinset [Re: Snazz]
    #23416214 - 07/06/16 07:49 AM (7 years, 6 months ago)

Personally I've never been a fan of consolidation, and fruit immediately at spawning.
These tubs were harvested 14 days after spawning

Instead of an extra two weeks just looking at my jars and subs could turn out two more of these.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Re: Lucky MS pinset [Re: NDStepp84]
    #23416351 - 07/06/16 09:03 AM (7 years, 6 months ago)

Anyone have any tips for a good pinset using BRF to manure/Coe/Verm. MS. Doubt I'll get good answers using MS. I've had one canopy out of about 9 tubs.


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Re: Lucky MS pinset [Re: NDStepp84]
    #23416368 - 07/06/16 09:09 AM (7 years, 6 months ago)

Very true.  Guess I should have prefaced that statement with 'A/PE testing'


Although more actives should be synthesized over a longer life cycle.  No idea still what function it had to the fungi though, usually makes it easier to figure out.


(like THC being a UV filter, so add some extra to increase production)


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Re: Lucky MS pinset [Re: Snazz]
    #23416441 - 07/06/16 10:07 AM (7 years, 6 months ago)

I have often wondered about time/potency as well. My thoughts were that there may be a link between not how long it takes to get pins, but how long it takes the fruit bodies themselves to mature may have something to do with producing more actives. PE/ APE for example, the fruits take longer to mature. I've had pins fast with both (10-14 days), but the fruits mature more slowly than normal cubes.

Also the tubs above, the mushrooms grew to maturity super fast and potency was nothing special, but I have an AA+ clone that the fruits are much slower to mature and the potency is above average.
Not saying that just because a mushroom grows slow or fast it is more or less potent 100% of the time, you can even grow bunk PE, but there may be something to it. Just a thought from observations of my limited experience.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Re: Lucky MS pinset [Re: NDStepp84]
    #23416477 - 07/06/16 10:20 AM (7 years, 6 months ago)

It makes perfect sense.  Most strains don't generate a ton of actives until knotting.


Fast growth may lead to mostly chitin and water.


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Re: Lucky MS pinset [Re: Snazz]
    #23416531 - 07/06/16 10:37 AM (7 years, 6 months ago)

Very interesting. Possibly a good way of testing could be growing MS tubs, start taking dated pictures when pins form, cloning growing out and testing the single fastest/slowest maturing mushrooms of each grow.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Re: Lucky MS pinset [Re: NDStepp84]
    #23416563 - 07/06/16 10:48 AM (7 years, 6 months ago)

Ya, I'm going to be photo journalling all 3 tubs. Cloning, labeling to pic# and bio-assays with harmaline to try and get a rough estimate of strength.


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Re: Lucky MS pinset [Re: Snazz]
    #23416828 - 07/06/16 12:33 PM (7 years, 6 months ago)

I gave it some bottom watering today so we'll see how that helps. Also I noticed I'm still getting some side pins even though I put blue painters tape around the tub to block light this time and am using a liner. Last time I didn't use tape and there were more pins on the side than the top.

Here's a pic from yesterday. This morning there was one lone mushroom with its veil already open.



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Re: Lucky MS pinset [Re: searching]
    #23416842 - 07/06/16 12:41 PM (7 years, 6 months ago)

Light is not the cause of side pins. Microclimate is.


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Re: Lucky MS pinset [Re: Pastywhyte]
    #23416858 - 07/06/16 12:48 PM (7 years, 6 months ago)

How can I prevent them then? Im using a liner and it's still stuck to the substrate with no gap, but still getting side pins. Thanks for the help, pasty. I appreciate it.


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Re: Lucky MS pinset [Re: searching]
    #23416868 - 07/06/16 12:51 PM (7 years, 6 months ago)

Use better liners. The real trick to getting liners to work is to not open the bag up, then when you trim off the edges you essentially have 2 layers of plastic. The outer layer sticks to the tub as most liners are prone to doing, but the inside liner pulls away and stays attached to the substrate. I also find "flexfit" bags work best as liners, the texture helps the sub grip them as it shrinks.


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Re: Lucky MS pinset [Re: Pastywhyte] * 1
    #23416873 - 07/06/16 12:55 PM (7 years, 6 months ago)

It's not cubes but this pic shows the idea. See how one of the bags is stuck to the tub at the top, but the inside liner held onto the sub. If I only used one liner that would have resulted in that section pulling away from the sub and it would be jammed with side pins.



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OfflineMycoLoopology
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Re: Lucky MS pinset [Re: Pastywhyte]
    #23416878 - 07/06/16 12:56 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Use better liners. The real trick to getting liners to work is to not open the bag up, then when you trim off the edges you essentially have 2 layers of plastic. The outer layer sticks to the tub as most liners are prone to doing, but the inside liner pulls away and stays attached to the substrate. I also find "flexfit" bags work best as liners, the texture helps the sub grip them as it shrinks.




well i know what ill be doing on my next tub, thanks buddy :laugh:

my tubs have always been hit or miss with one layer. ill have pockets here and there where the sub didnt connect to the liner as well. i can see this being a neat little trick to fix that lol. surprised i havent heard this sooner!


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Re: Lucky MS pinset [Re: searching]
    #23416932 - 07/06/16 01:18 PM (7 years, 6 months ago)

Congrats man, I'm looking forward until the day I get a pinset like you! Do you used a casing layer? Cause I read that it should be done when fruiting on spawning.


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Re: Lucky MS pinset [Re: Pastywhyte]
    #23417974 - 07/06/16 06:34 PM (7 years, 6 months ago)

Quote:

radhaz said:
Congrats man, I'm looking forward until the day I get a pinset like you! Do you used a casing layer? Cause I read that it should be done when fruiting on spawning.




I did not use a casing layer. Just a very thin layer of sub sprinkled on the top and evened out.

Quote:

Pastywhyte said:
It's not cubes but this pic shows the idea. See how one of the bags is stuck to the tub at the top, but the inside liner held onto the sub. If I only used one liner that would have resulted in that section pulling away from the sub and it would be jammed with side pins.






Thanks! I'll do that.



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Re: Lucky MS pinset [Re: searching]
    #23417993 - 07/06/16 06:40 PM (7 years, 6 months ago)

I picked the ones with the veils open and they are all about 3 inches long it's funny. I wish they would have been taller but I almost don't care because they are so cute like mini shrooms. :rofl:

:girlishjoy:



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Re: Lucky MS pinset [Re: searching]
    #23419352 - 07/07/16 06:59 AM (7 years, 6 months ago)

And it's done. Hundreds of mini mushrooms.



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InvisibleComebackKid
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Re: Lucky MS pinset [Re: searching]
    #23420112 - 07/07/16 12:39 PM (7 years, 6 months ago)

:thumbup: still pretty sexy
What was your yield?


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