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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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wont fucking grow
#23409852 - 07/04/16 10:15 AM (7 years, 6 months ago) |
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I have no growth or contams its been like almost two weeks some jars are at Day 10 but no growth so what do you guys thibk i thought it only takes like 21 Days
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Bazookatooth
Jarl of Burger King

Registered: 04/09/15
Posts: 250
Last seen: 3 years, 6 months
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I just had a bunch of jars show zero growth bad or good l too am interested as too why this happens.
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wayfaytoolive23
Stranger

Registered: 07/31/13
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Last seen: 7 years, 1 month
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How long did your jars show no growth for ?
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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it could be bacteria. does it look wet in there? BRF cakes hide contams very well a lot of times. It could also be old spores, you could have used too much spore solution, there are a few factors that could be at play here. wait at least another week before chucking 'em to be sure the spores weren't just a bit old.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Some of my cakes look kinda wet but nothing to serious but they dont smell bad at all And i can sens pics
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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it can take like 2 weeks for spores to germinate and show growth, sometimes. just be patient.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Bazookatooth
Jarl of Burger King

Registered: 04/09/15
Posts: 250
Last seen: 3 years, 6 months
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I use Rye, inoculate 20 jars, 4 varieties. Of the 20 jars 15 started showing mycelium while the rest did nothing for a week and a half. Around then the hard were around 30% colonized and nothing in the jars. I opened them up to dump and use for transfers and they had no funky smell, just clean rye.
I will definitely be a lot more patient in the future!
Edited by Bazookatooth (07/04/16 10:48 AM)
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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ok, but your mans is talkin' about cakes. if you're shooting up your rye jars with spore solution, that could very well be your problem.
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Bazookatooth
Jarl of Burger King

Registered: 04/09/15
Posts: 250
Last seen: 3 years, 6 months
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Didn't read where he was talking about cakes till now, thanks!
Could you please elaborate on the rye problem?
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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spore solution is typically aseptic, but not sterile. just like not wiping after taking a shit, you may be able to get away with it once in a while, but eventually you'll get skid marks (that's an RRism).
to test, you can try dropping (i mean 1-2 drops) solution on the top of your PC'd grains. If clean enough, you'll start seeing the growth where you dropped the solution only (unless you move the jars enough to shake the solution down. Even that's far from fool proof, but may help you in your situ.
typically, we like to clean spore growth on agar first, before inoculating grain. in the long run, you'd save a lot of time and money doing so.
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Bazookatooth
Jarl of Burger King

Registered: 04/09/15
Posts: 250
Last seen: 3 years, 6 months
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Oh I understand now. I've done some sucessful agar work in the past it's time I ramp back up then.
Thanks for the response man, clears a lot up
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Its been 3 days and no sign of growth in any of my jars what do you guys think Do you think they will ever grow some mycliume
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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three days is far too soon to get worried, man.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Yea but ive had them for like a week already its been 3 days from a week And they dont smell bad or even have any signs of contams
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Just wait it out. It will be done with before you know it. I do recordment a little culture work in your free time. It will come in handy with speed later on.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Re: wont fucking grow [Re: tump]
#23425153 - 07/08/16 10:53 PM (7 years, 6 months ago) |
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Give me a link
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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A link to agar, blender less Li, liquid cultures. What are you asking for boss
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: wont fucking grow [Re: tump]
#23425226 - 07/08/16 11:28 PM (7 years, 6 months ago) |
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
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Re: wont fucking grow [Re: Inocuole]
#23425342 - 07/09/16 12:46 AM (7 years, 6 months ago) |
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I've never seen ores take longer than a week to germinate. Especially in every inoculation site across all jars. You should prepare to start over
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (07/09/16 12:47 AM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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You're lucky. Longest I've waited and actually was successful was 2 months. They were the most bitchiest spores ive ever had. Luckily I got prints and cultures now so... yay 
I gave up at the 3 week mark, but was too lazy to toss. Checked in at 2 months, and I saw about a dime sized spot on a pasty plate
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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That sucks balls. Longest I've had so far (which I didn't give up on) was a little over 2 weeks and it nearly drove me insane.
I have a couple of prints which I recieved in a trade that simply won't fucking germinate. I have a plate of each on soft agar and warm poured with soft agar yet again, it's been at least 3 weeks but I'm not expecting anything from these, I suspect the spores are dead. The prints are very strange, all the spores are stuck together and whenever I open the foil, scabs of clumped spores fall out The guy who sent them to me probably fucked them up somehow.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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This is what my setup looks like idk why i hvaent had any growrh?
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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what's that? a heater?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Also how do you expect to get them out of those jars? Thy look like brf cakes right?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Well of course, it's only summer you know. How else are we gonna get a core temp of 90F? 
Quote:
Mad Season said: Also how do you expect to get them out of those jars? Thy look like brf cakes right?
Why break the jars, of course.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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The temp stays at like 78 and yes its a heater and there brf cakes
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Mushiez



Registered: 04/28/14
Posts: 1,057
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Quote:
wayfaytoolive23 said: The temp stays at like 78 and yes its a heater and there brf cakes
ditch the heater unless it's freezing in your place
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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so um, you just opened the lid and squirted?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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This is gonna end badly. There is no way that those cakes are coming out of the jars in one piece for one. The heater will probably make them bacterial as fuck and stall as well. If by some miracle they do survive the near desert conditions, the only thing you can do is crumble them up and spawn them to coir.
You need to do a ton of reading OP.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Why wont they come out and they have no contams at all
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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The heater keeps things kinda warm
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
wayfaytoolive23 said: Why wont they come out and they have no contams at all so far
XD fixed. Sorry I do hope it doesn't contam, but never assume it's clean til the fat lady sings.
Having it at 78 is borderline too hot.. I really would never go past 80. 75-81 on petri dishes (2-D surface without thermogenesis) is apparently the fastest growth RR had. In cakes which are 3-D, you will have to lower temps for thermogenesis. Also high temps just invite contams. Especially if there's dormant endospores that survived the PC, or other contams in your syringes.
Edited by Mad Season (07/09/16 08:44 PM)
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Yea but what does he mean i will never get them out in one peace
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Just wanted to say that this gave me a really good laugh.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Because the jar is regular mouth. A cake will be dense as fuck once colonized, even with wide mouth jars they can be problematic to remove. There is no way you will be able to get those out in one piece. Regular mouth jars are only used for grain jars because those can be broken apart.
Just because you can't see a contam doesn't mean there isn't any. If it's as hot there as it is here, a heater is just a guarateed bacterial contamination. You should only use an incubator/heater if the temps drop below 60F. 70-73F room/incubator temp is the best colonization temperature, anything above that will give contams the upper hand, especially bacteria.
Also, if you are using spores to inoculate your jars, they will never be 100% clean. Spore solutions are only aseptic and never really sterile.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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So what your saying is i have the wrong sized jars
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Not wrong size, wrong type of jar.
 Regular mouth jars, normally used for anything but cakes. See how the mouth is smaller than the rest of the jar? This is the wrong type for cakes because the cake will be wider than the mouth of the jar so you have to break it apart to get it out.
  Widemouth jars. These are the jars you should be using for cakes. The mouth of the jar is the same diameter as the rest of the jar. Only with these jars can you get cakes out in a single piece.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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your jars have shoulders. it's a simple matter of logistics.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Fuck it i already bought the ones i have i will use them and brake them At the end and then get the other ones your talking about
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Or crumble the cakes and spawn them to some coir. Better than wasting a perfectly good jar. Plus, you get a taste of how the big boys grow.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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How exactly would that work is it the same to fruit
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Yeah, you basically skip the consolidation, break the cake apart to get it out and use a cheese grater to crumble it. Mix it with some coco coir in a tray and let it colonize again in your FC.
It's basically doing bulk but rather than colonizing grains, you use a crumbled cake as your spawn.
check out the bucket tek to see how to prep coir. You should use a spawn ratio of 1:1 or 1:2. this means that for every half pint jar cake, you can use anywhere from 1-2 same sized jar of coir and verm. It's quite simple with coir and you can't really go wrong. Bulk is in many ways superior to cakes IMO, you might end up having more fun with it than you have with cakes.
You can also follow a number of other teks, Sloppy's big gulp comes to mind. For example, if you want to use the gulp tek, skip the parts until you get to "sub prep". Alternatively, you can use the bucket tek for prepping coir if you think it's easier.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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i actually just did Sloppy's Big gulp with two brf AA+ jars i had. so 4 Gulps, 2 Gulps to 1 half pint brf cake, at a 1:1.5ish spawn:sub ratio. 2 have fully colonized the casing, so should be pinning any minute, and the other two are close behind.
Here they are, right after i mixed them:

Ill be posting results on Sloppy's Gulp Tek thread, when the time comes, if anyone is interested to see how brf does.
I am actually updating today, since I have been gone for the last week.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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