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Offlinewayfaytoolive23
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wont fucking grow
    #23409852 - 07/04/16 10:15 AM (7 years, 6 months ago)

I have no growth or contams its been like almost two weeks some jars are at
Day 10 but no growth so what do you guys thibk i thought it only takes like 21
Days


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OfflineBazookatooth
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23409867 - 07/04/16 10:21 AM (7 years, 6 months ago)

I just had a bunch of jars show zero growth bad or good l too am interested as too why this happens.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Bazookatooth]
    #23409874 - 07/04/16 10:24 AM (7 years, 6 months ago)

How long did your jars show no growth for ?


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Re: wont fucking grow [Re: Bazookatooth]
    #23409876 - 07/04/16 10:25 AM (7 years, 6 months ago)

it could be bacteria. does it look wet in there?
BRF cakes hide contams very well a lot of times. It could also be old spores, you could have used too much spore solution, there are a few factors that could be at play here. wait at least another week before chucking 'em to be sure the spores weren't just a bit old.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23409884 - 07/04/16 10:28 AM (7 years, 6 months ago)

Some of my cakes look kinda wet but nothing to serious but they dont smell bad at all
And i can sens pics


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23409905 - 07/04/16 10:35 AM (7 years, 6 months ago)

it can take like 2 weeks for spores to germinate and show growth, sometimes. just be patient.


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OfflineBazookatooth
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Re: wont fucking grow [Re: mupetmower]
    #23409939 - 07/04/16 10:47 AM (7 years, 6 months ago)

I use Rye, inoculate 20 jars, 4 varieties. Of the 20 jars 15 started showing mycelium while the rest did nothing for a week and a half. Around then the hard were around 30% colonized and nothing in the jars. I opened them up to dump and use for transfers and they had no funky smell, just clean rye.

I will definitely be a lot more patient in the future!


Edited by Bazookatooth (07/04/16 10:48 AM)


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Invisibledankington
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Re: wont fucking grow [Re: Bazookatooth]
    #23409946 - 07/04/16 10:50 AM (7 years, 6 months ago)

ok, but your mans is talkin' about cakes.
if you're shooting up your rye jars with spore solution, that could very well be your problem.


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OfflineBazookatooth
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Re: wont fucking grow [Re: dankington]
    #23409956 - 07/04/16 10:52 AM (7 years, 6 months ago)

Didn't read where he was talking about cakes till now, thanks!

Could you please elaborate on the rye problem?


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Re: wont fucking grow [Re: Bazookatooth]
    #23409985 - 07/04/16 11:02 AM (7 years, 6 months ago)

spore solution is typically aseptic, but not sterile. just like not wiping after taking a shit, you may be able to get away with it once in a while, but eventually you'll get skid marks (that's an RRism).

to test, you can try dropping (i mean 1-2 drops) solution on the top of your PC'd grains. If clean enough, you'll start seeing the growth where you dropped the solution only (unless you move the jars enough to shake the solution down. Even that's far from fool proof, but may help you in your situ.

typically, we like to clean spore growth on agar first, before inoculating grain. in the long run, you'd save a lot of time and money doing so.


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OfflineBazookatooth
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Re: wont fucking grow [Re: dankington]
    #23410014 - 07/04/16 11:14 AM (7 years, 6 months ago)

Oh I understand now. I've done some sucessful agar work in the past it's time I ramp back up then.

Thanks for the response man, clears a lot up


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Bazookatooth]
    #23424754 - 07/08/16 08:45 PM (7 years, 6 months ago)

Its been 3 days and no sign of growth in any of my jars what do you guys think
Do you think they will ever grow some mycliume


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23424758 - 07/08/16 08:48 PM (7 years, 6 months ago)

three days is far too soon to get worried, man.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: dankington]
    #23424787 - 07/08/16 09:01 PM (7 years, 6 months ago)

Yea but ive had them for like a week already its been 3 days from a week
And they dont smell bad or even have any signs of contams


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23425096 - 07/08/16 10:36 PM (7 years, 6 months ago)

Just wait it out. It will be done with before you know it. I do recordment a little culture work in your free time. It will come in handy with speed later on.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: tump]
    #23425153 - 07/08/16 10:53 PM (7 years, 6 months ago)

Give me a link


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23425199 - 07/08/16 11:12 PM (7 years, 6 months ago)

A link to agar, blender less Li, liquid cultures. What are you asking for boss


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Re: wont fucking grow [Re: tump]
    #23425226 - 07/08/16 11:28 PM (7 years, 6 months ago)



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Re: wont fucking grow [Re: Inocuole]
    #23425342 - 07/09/16 12:46 AM (7 years, 6 months ago)

I've never seen ores take longer than a week to germinate. Especially in every inoculation site across all jars. You should prepare to start over


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Edited by Mycologist217 (07/09/16 12:47 AM)


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InvisibleMad Season
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Re: wont fucking grow [Re: Mycologist217]
    #23425346 - 07/09/16 12:53 AM (7 years, 6 months ago)

You're lucky. Longest I've waited and actually was successful was 2 months. They were the most bitchiest spores ive ever had. Luckily I got prints and cultures now so... yay :smile:

I gave up at the 3 week mark, but was too lazy to toss. Checked in at 2 months, and I saw about a dime sized spot on a pasty plate


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Re: wont fucking grow [Re: Mad Season]
    #23425689 - 07/09/16 05:23 AM (7 years, 6 months ago)

That sucks balls. Longest I've had so far (which I didn't give up on) was a little over 2 weeks and it nearly drove me insane.

I have a couple of prints which I recieved in a trade that simply won't fucking germinate. I have a plate of each on soft agar and warm poured with soft agar yet again, it's been at least 3 weeks but I'm not expecting anything from these, I suspect the spores are dead. The prints are very strange, all the spores are stuck together and whenever I open the foil, scabs of clumped spores fall out :lol: The guy who sent them to me probably fucked them up somehow.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23426939 - 07/09/16 03:21 PM (7 years, 6 months ago)

This is what my setup looks like idk why i hvaent had any growrh?





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Re: wont fucking grow [Re: wayfaytoolive23]
    #23426942 - 07/09/16 03:24 PM (7 years, 6 months ago)

:notsureif: what's that? a heater?


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Re: wont fucking grow [Re: dankington]
    #23426947 - 07/09/16 03:28 PM (7 years, 6 months ago)

Also how do you expect to get them out of those jars? Thy look like brf cakes right?


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Re: wont fucking grow [Re: dankington]
    #23426949 - 07/09/16 03:28 PM (7 years, 6 months ago)

Well of course, it's only summer you know. How else are we gonna get a core temp of 90F? :lol:

Quote:

Mad Season said:
Also how do you expect to get them out of those jars? Thy look like brf cakes right?




Why break the jars, of course.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23426954 - 07/09/16 03:31 PM (7 years, 6 months ago)

The temp stays at like 78 and yes its a heater and there brf cakes


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23426963 - 07/09/16 03:34 PM (7 years, 6 months ago)

Quote:

wayfaytoolive23 said:
The temp stays at like 78 and yes its a heater and there brf cakes




ditch the heater unless it's freezing in your place


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23426967 - 07/09/16 03:35 PM (7 years, 6 months ago)

:confused2: so um, you just opened the lid and squirted?


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Re: wont fucking grow [Re: dankington]
    #23426976 - 07/09/16 03:40 PM (7 years, 6 months ago)

:lol: This is gonna end badly. There is no way that those cakes are coming out of the jars in one piece for one. The heater will probably make them bacterial as fuck and stall as well. If by some miracle they do survive the near desert conditions, the only thing you can do is crumble them up and spawn them to coir.

You need to do a ton of reading OP.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427684 - 07/09/16 08:38 PM (7 years, 6 months ago)

Why wont they come out and they have no contams at all


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427687 - 07/09/16 08:39 PM (7 years, 6 months ago)

The heater keeps things kinda warm


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427690 - 07/09/16 08:41 PM (7 years, 6 months ago)

Quote:

wayfaytoolive23 said:
Why wont they come out and they have no contams at all so far



XD fixed. Sorry I do hope it doesn't contam, but never assume it's clean til the fat lady sings.

Having it at 78 is borderline too hot.. I really would never go past 80. 75-81 on petri dishes (2-D surface without thermogenesis) is apparently the fastest growth RR had. In cakes which are 3-D, you will have to lower temps for thermogenesis. Also high temps just invite contams. Especially if there's dormant endospores that survived the PC, or other contams in your syringes.


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Edited by Mad Season (07/09/16 08:44 PM)


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Mad Season]
    #23427695 - 07/09/16 08:43 PM (7 years, 6 months ago)

Yea but what does he mean i will never get them out in one peace


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Re: wont fucking grow [Re: Mad Season]
    #23427696 - 07/09/16 08:43 PM (7 years, 6 months ago)

Just wanted to say that this gave me a really good laugh.


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427697 - 07/09/16 08:44 PM (7 years, 6 months ago)

Because the jar is regular mouth. A cake will be dense as fuck once colonized, even with wide mouth jars they can be problematic to remove. There is no way you will be able to get those out in one piece. Regular mouth jars are only used for grain jars because those can be broken apart.

Just because you can't see a contam doesn't mean there isn't any. If it's as hot there as it is here, a heater is just a guarateed bacterial contamination. You should only use an incubator/heater if the temps drop below 60F. 70-73F room/incubator temp is the best colonization temperature, anything above that will give contams the upper hand, especially bacteria.

Also, if you are using spores to inoculate your jars, they will never be 100% clean. Spore solutions are only aseptic and never really sterile.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427707 - 07/09/16 08:47 PM (7 years, 6 months ago)

So what your saying is i have the wrong sized jars


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427720 - 07/09/16 08:53 PM (7 years, 6 months ago)

Not wrong size, wrong type of jar.


Regular mouth jars, normally used for anything but cakes. See how the mouth is smaller than the rest of the jar? This is the wrong type for cakes because the cake will be wider than the mouth of the jar so you have to break it apart to get it out.

Widemouth jars. These are the jars you should be using for cakes. The mouth of the jar is the same diameter as the rest of the jar. Only with these jars can you get cakes out in a single piece.


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427721 - 07/09/16 08:53 PM (7 years, 6 months ago)

your jars have shoulders. it's a simple matter of logistics.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427727 - 07/09/16 08:56 PM (7 years, 6 months ago)

Fuck it i already bought the ones i have i will use them and brake them
At the end and then get the other ones your talking about


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427735 - 07/09/16 08:58 PM (7 years, 6 months ago)

Or crumble the cakes and spawn them to some coir. Better than wasting a perfectly good jar. Plus, you get a taste of how the big boys grow.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427736 - 07/09/16 08:59 PM (7 years, 6 months ago)

How exactly would that work is it the same to fruit


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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427768 - 07/09/16 09:11 PM (7 years, 6 months ago)

Yeah, you basically skip the consolidation, break the cake apart to get it out and use a cheese grater to crumble it. Mix it with some coco coir in a tray and let it colonize again in your FC.

It's basically doing bulk but rather than colonizing grains, you use a crumbled cake as your spawn.

check out the bucket tek to see how to prep coir. You should use a spawn ratio of 1:1 or 1:2. this means that for every half pint jar cake, you can use anywhere from 1-2 same sized jar of coir and verm. It's quite simple with coir and you can't really go wrong. Bulk is in many ways superior to cakes IMO, you might end up having more fun with it than you have with cakes.

You can also follow a number of other teks, Sloppy's big gulp comes to mind. For example, if you want to use the gulp tek, skip the parts until you get to "sub prep". Alternatively, you can use the bucket tek for prepping coir if you think it's easier.


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Re: wont fucking grow [Re: Supalemonhaze]
    #23428952 - 07/10/16 10:37 AM (7 years, 6 months ago)

i actually just did Sloppy's Big gulp with two brf AA+ jars i had. so 4 Gulps, 2 Gulps to 1 half pint brf cake, at a 1:1.5ish spawn:sub ratio. 2 have fully colonized the casing, so should be pinning any minute, and the other two are close behind.

Here they are, right after i mixed them:


Ill be posting results on Sloppy's Gulp Tek thread, when the time comes, if anyone is interested to see how brf does.


I am actually updating today, since I have been gone for the last week.


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