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InvisibleSupalemonhaze
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Re: wont fucking grow [Re: Mad Season]
    #23425689 - 07/09/16 05:23 AM (7 years, 6 months ago)

That sucks balls. Longest I've had so far (which I didn't give up on) was a little over 2 weeks and it nearly drove me insane.

I have a couple of prints which I recieved in a trade that simply won't fucking germinate. I have a plate of each on soft agar and warm poured with soft agar yet again, it's been at least 3 weeks but I'm not expecting anything from these, I suspect the spores are dead. The prints are very strange, all the spores are stuck together and whenever I open the foil, scabs of clumped spores fall out :lol: The guy who sent them to me probably fucked them up somehow.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23426939 - 07/09/16 03:21 PM (7 years, 6 months ago)

This is what my setup looks like idk why i hvaent had any growrh?





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Invisibledankington
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23426942 - 07/09/16 03:24 PM (7 years, 6 months ago)

:notsureif: what's that? a heater?


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InvisibleMad Season
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Re: wont fucking grow [Re: dankington]
    #23426947 - 07/09/16 03:28 PM (7 years, 6 months ago)

Also how do you expect to get them out of those jars? Thy look like brf cakes right?


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InvisibleSupalemonhaze
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Re: wont fucking grow [Re: dankington]
    #23426949 - 07/09/16 03:28 PM (7 years, 6 months ago)

Well of course, it's only summer you know. How else are we gonna get a core temp of 90F? :lol:

Quote:

Mad Season said:
Also how do you expect to get them out of those jars? Thy look like brf cakes right?




Why break the jars, of course.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23426954 - 07/09/16 03:31 PM (7 years, 6 months ago)

The temp stays at like 78 and yes its a heater and there brf cakes


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InvisibleMushiez
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23426963 - 07/09/16 03:34 PM (7 years, 6 months ago)

Quote:

wayfaytoolive23 said:
The temp stays at like 78 and yes its a heater and there brf cakes




ditch the heater unless it's freezing in your place


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Invisibledankington
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23426967 - 07/09/16 03:35 PM (7 years, 6 months ago)

:confused2: so um, you just opened the lid and squirted?


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InvisibleSupalemonhaze
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Re: wont fucking grow [Re: dankington]
    #23426976 - 07/09/16 03:40 PM (7 years, 6 months ago)

:lol: This is gonna end badly. There is no way that those cakes are coming out of the jars in one piece for one. The heater will probably make them bacterial as fuck and stall as well. If by some miracle they do survive the near desert conditions, the only thing you can do is crumble them up and spawn them to coir.

You need to do a ton of reading OP.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427684 - 07/09/16 08:38 PM (7 years, 6 months ago)

Why wont they come out and they have no contams at all


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427687 - 07/09/16 08:39 PM (7 years, 6 months ago)

The heater keeps things kinda warm


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InvisibleMad Season
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427690 - 07/09/16 08:41 PM (7 years, 6 months ago)

Quote:

wayfaytoolive23 said:
Why wont they come out and they have no contams at all so far



XD fixed. Sorry I do hope it doesn't contam, but never assume it's clean til the fat lady sings.

Having it at 78 is borderline too hot.. I really would never go past 80. 75-81 on petri dishes (2-D surface without thermogenesis) is apparently the fastest growth RR had. In cakes which are 3-D, you will have to lower temps for thermogenesis. Also high temps just invite contams. Especially if there's dormant endospores that survived the PC, or other contams in your syringes.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/09/16 08:44 PM)


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Mad Season]
    #23427695 - 07/09/16 08:43 PM (7 years, 6 months ago)

Yea but what does he mean i will never get them out in one peace


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Invisiblemupetmower
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Re: wont fucking grow [Re: Mad Season]
    #23427696 - 07/09/16 08:43 PM (7 years, 6 months ago)

Just wanted to say that this gave me a really good laugh.


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-The wise man never stops seeking knowledge.


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InvisibleSupalemonhaze
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427697 - 07/09/16 08:44 PM (7 years, 6 months ago)

Because the jar is regular mouth. A cake will be dense as fuck once colonized, even with wide mouth jars they can be problematic to remove. There is no way you will be able to get those out in one piece. Regular mouth jars are only used for grain jars because those can be broken apart.

Just because you can't see a contam doesn't mean there isn't any. If it's as hot there as it is here, a heater is just a guarateed bacterial contamination. You should only use an incubator/heater if the temps drop below 60F. 70-73F room/incubator temp is the best colonization temperature, anything above that will give contams the upper hand, especially bacteria.

Also, if you are using spores to inoculate your jars, they will never be 100% clean. Spore solutions are only aseptic and never really sterile.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427707 - 07/09/16 08:47 PM (7 years, 6 months ago)

So what your saying is i have the wrong sized jars


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InvisibleSupalemonhaze
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427720 - 07/09/16 08:53 PM (7 years, 6 months ago)

Not wrong size, wrong type of jar.


Regular mouth jars, normally used for anything but cakes. See how the mouth is smaller than the rest of the jar? This is the wrong type for cakes because the cake will be wider than the mouth of the jar so you have to break it apart to get it out.

Widemouth jars. These are the jars you should be using for cakes. The mouth of the jar is the same diameter as the rest of the jar. Only with these jars can you get cakes out in a single piece.


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Invisibledankington
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427721 - 07/09/16 08:53 PM (7 years, 6 months ago)

your jars have shoulders. it's a simple matter of logistics.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Supalemonhaze]
    #23427727 - 07/09/16 08:56 PM (7 years, 6 months ago)

Fuck it i already bought the ones i have i will use them and brake them
At the end and then get the other ones your talking about


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23427735 - 07/09/16 08:58 PM (7 years, 6 months ago)

Or crumble the cakes and spawn them to some coir. Better than wasting a perfectly good jar. Plus, you get a taste of how the big boys grow.


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