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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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That sucks balls. Longest I've had so far (which I didn't give up on) was a little over 2 weeks and it nearly drove me insane.
I have a couple of prints which I recieved in a trade that simply won't fucking germinate. I have a plate of each on soft agar and warm poured with soft agar yet again, it's been at least 3 weeks but I'm not expecting anything from these, I suspect the spores are dead. The prints are very strange, all the spores are stuck together and whenever I open the foil, scabs of clumped spores fall out The guy who sent them to me probably fucked them up somehow.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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This is what my setup looks like idk why i hvaent had any growrh?
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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what's that? a heater?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Also how do you expect to get them out of those jars? Thy look like brf cakes right?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Well of course, it's only summer you know. How else are we gonna get a core temp of 90F? 
Quote:
Mad Season said: Also how do you expect to get them out of those jars? Thy look like brf cakes right?
Why break the jars, of course.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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The temp stays at like 78 and yes its a heater and there brf cakes
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Mushiez



Registered: 04/28/14
Posts: 1,057
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Quote:
wayfaytoolive23 said: The temp stays at like 78 and yes its a heater and there brf cakes
ditch the heater unless it's freezing in your place
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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so um, you just opened the lid and squirted?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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This is gonna end badly. There is no way that those cakes are coming out of the jars in one piece for one. The heater will probably make them bacterial as fuck and stall as well. If by some miracle they do survive the near desert conditions, the only thing you can do is crumble them up and spawn them to coir.
You need to do a ton of reading OP.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Why wont they come out and they have no contams at all
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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The heater keeps things kinda warm
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
wayfaytoolive23 said: Why wont they come out and they have no contams at all so far
XD fixed. Sorry I do hope it doesn't contam, but never assume it's clean til the fat lady sings.
Having it at 78 is borderline too hot.. I really would never go past 80. 75-81 on petri dishes (2-D surface without thermogenesis) is apparently the fastest growth RR had. In cakes which are 3-D, you will have to lower temps for thermogenesis. Also high temps just invite contams. Especially if there's dormant endospores that survived the PC, or other contams in your syringes.
Edited by Mad Season (07/09/16 08:44 PM)
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Yea but what does he mean i will never get them out in one peace
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Just wanted to say that this gave me a really good laugh.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Because the jar is regular mouth. A cake will be dense as fuck once colonized, even with wide mouth jars they can be problematic to remove. There is no way you will be able to get those out in one piece. Regular mouth jars are only used for grain jars because those can be broken apart.
Just because you can't see a contam doesn't mean there isn't any. If it's as hot there as it is here, a heater is just a guarateed bacterial contamination. You should only use an incubator/heater if the temps drop below 60F. 70-73F room/incubator temp is the best colonization temperature, anything above that will give contams the upper hand, especially bacteria.
Also, if you are using spores to inoculate your jars, they will never be 100% clean. Spore solutions are only aseptic and never really sterile.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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So what your saying is i have the wrong sized jars
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Not wrong size, wrong type of jar.
 Regular mouth jars, normally used for anything but cakes. See how the mouth is smaller than the rest of the jar? This is the wrong type for cakes because the cake will be wider than the mouth of the jar so you have to break it apart to get it out.
  Widemouth jars. These are the jars you should be using for cakes. The mouth of the jar is the same diameter as the rest of the jar. Only with these jars can you get cakes out in a single piece.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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your jars have shoulders. it's a simple matter of logistics.
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wayfaytoolive23
Stranger

Registered: 07/31/13
Posts: 117
Last seen: 7 years, 1 month
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Fuck it i already bought the ones i have i will use them and brake them At the end and then get the other ones your talking about
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Or crumble the cakes and spawn them to some coir. Better than wasting a perfectly good jar. Plus, you get a taste of how the big boys grow.
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