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Offlinewayfaytoolive23
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wont fucking grow
    #23409852 - 07/04/16 10:15 AM (7 years, 6 months ago)

I have no growth or contams its been like almost two weeks some jars are at
Day 10 but no growth so what do you guys thibk i thought it only takes like 21
Days


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OfflineBazookatooth
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23409867 - 07/04/16 10:21 AM (7 years, 6 months ago)

I just had a bunch of jars show zero growth bad or good l too am interested as too why this happens.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Bazookatooth]
    #23409874 - 07/04/16 10:24 AM (7 years, 6 months ago)

How long did your jars show no growth for ?


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Invisibledankington
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Re: wont fucking grow [Re: Bazookatooth]
    #23409876 - 07/04/16 10:25 AM (7 years, 6 months ago)

it could be bacteria. does it look wet in there?
BRF cakes hide contams very well a lot of times. It could also be old spores, you could have used too much spore solution, there are a few factors that could be at play here. wait at least another week before chucking 'em to be sure the spores weren't just a bit old.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23409884 - 07/04/16 10:28 AM (7 years, 6 months ago)

Some of my cakes look kinda wet but nothing to serious but they dont smell bad at all
And i can sens pics


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Invisiblemupetmower
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23409905 - 07/04/16 10:35 AM (7 years, 6 months ago)

it can take like 2 weeks for spores to germinate and show growth, sometimes. just be patient.


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OfflineBazookatooth
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Re: wont fucking grow [Re: mupetmower]
    #23409939 - 07/04/16 10:47 AM (7 years, 6 months ago)

I use Rye, inoculate 20 jars, 4 varieties. Of the 20 jars 15 started showing mycelium while the rest did nothing for a week and a half. Around then the hard were around 30% colonized and nothing in the jars. I opened them up to dump and use for transfers and they had no funky smell, just clean rye.

I will definitely be a lot more patient in the future!


Edited by Bazookatooth (07/04/16 10:48 AM)


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Invisibledankington
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Re: wont fucking grow [Re: Bazookatooth]
    #23409946 - 07/04/16 10:50 AM (7 years, 6 months ago)

ok, but your mans is talkin' about cakes.
if you're shooting up your rye jars with spore solution, that could very well be your problem.


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OfflineBazookatooth
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Re: wont fucking grow [Re: dankington]
    #23409956 - 07/04/16 10:52 AM (7 years, 6 months ago)

Didn't read where he was talking about cakes till now, thanks!

Could you please elaborate on the rye problem?


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Invisibledankington
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Re: wont fucking grow [Re: Bazookatooth]
    #23409985 - 07/04/16 11:02 AM (7 years, 6 months ago)

spore solution is typically aseptic, but not sterile. just like not wiping after taking a shit, you may be able to get away with it once in a while, but eventually you'll get skid marks (that's an RRism).

to test, you can try dropping (i mean 1-2 drops) solution on the top of your PC'd grains. If clean enough, you'll start seeing the growth where you dropped the solution only (unless you move the jars enough to shake the solution down. Even that's far from fool proof, but may help you in your situ.

typically, we like to clean spore growth on agar first, before inoculating grain. in the long run, you'd save a lot of time and money doing so.


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OfflineBazookatooth
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Re: wont fucking grow [Re: dankington]
    #23410014 - 07/04/16 11:14 AM (7 years, 6 months ago)

Oh I understand now. I've done some sucessful agar work in the past it's time I ramp back up then.

Thanks for the response man, clears a lot up


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: Bazookatooth]
    #23424754 - 07/08/16 08:45 PM (7 years, 6 months ago)

Its been 3 days and no sign of growth in any of my jars what do you guys think
Do you think they will ever grow some mycliume


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Invisibledankington
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23424758 - 07/08/16 08:48 PM (7 years, 6 months ago)

three days is far too soon to get worried, man.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: dankington]
    #23424787 - 07/08/16 09:01 PM (7 years, 6 months ago)

Yea but ive had them for like a week already its been 3 days from a week
And they dont smell bad or even have any signs of contams


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Offlinetump
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23425096 - 07/08/16 10:36 PM (7 years, 6 months ago)

Just wait it out. It will be done with before you know it. I do recordment a little culture work in your free time. It will come in handy with speed later on.


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Offlinewayfaytoolive23
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Re: wont fucking grow [Re: tump]
    #23425153 - 07/08/16 10:53 PM (7 years, 6 months ago)

Give me a link


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Offlinetump
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Re: wont fucking grow [Re: wayfaytoolive23]
    #23425199 - 07/08/16 11:12 PM (7 years, 6 months ago)

A link to agar, blender less Li, liquid cultures. What are you asking for boss


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InvisibleInocuole
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Re: wont fucking grow [Re: tump]
    #23425226 - 07/08/16 11:28 PM (7 years, 6 months ago)



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OfflineMycologist217
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Re: wont fucking grow [Re: Inocuole]
    #23425342 - 07/09/16 12:46 AM (7 years, 6 months ago)

I've never seen ores take longer than a week to germinate. Especially in every inoculation site across all jars. You should prepare to start over


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Edited by Mycologist217 (07/09/16 12:47 AM)


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InvisibleMad Season
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Re: wont fucking grow [Re: Mycologist217]
    #23425346 - 07/09/16 12:53 AM (7 years, 6 months ago)

You're lucky. Longest I've waited and actually was successful was 2 months. They were the most bitchiest spores ive ever had. Luckily I got prints and cultures now so... yay :smile:

I gave up at the 3 week mark, but was too lazy to toss. Checked in at 2 months, and I saw about a dime sized spot on a pasty plate


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