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InvisibleBoogieman47
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contams on mea??
    #23406946 - 07/03/16 12:21 PM (7 years, 6 months ago)

Ever since I started mea I've been getting this weird growth this is a b+ I just inoculated now the other two haven't done anything I did them at the same time in front of my hood I was using pda and never had an issue like this and the only thing that changed was the agar recipe I PC for 45 minutes cause I use sticks of agar ... but I had inoculated a mazatapec plate and it just dried out and left something like that on there and I know the syringe is clean I've used them before and they been in my fridge since I got them in March ... the recipe I use is 30 g malt 20 g agar and 1000 ml water


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Invisibleweetsie
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Re: contams on mea?? [Re: Boogieman47]
    #23406972 - 07/03/16 12:29 PM (7 years, 6 months ago)

30g is pretty rich for MEA, 20g per 1L is more normal and for spores I use 10g MEA 10g agar per 1L. They seem to have an easier time getting started on that.


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InvisibleBoogieman47
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Re: contams on mea?? [Re: weetsie]
    #23406976 - 07/03/16 12:32 PM (7 years, 6 months ago)

So you think the mea is just over powering the spores? It smelled pretty funky in there


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InvisibleLocN9ne
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Re: contams on mea?? [Re: Boogieman47]
    #23407011 - 07/03/16 12:44 PM (7 years, 6 months ago)

That sure does look like a pretty bacterial syringe...


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InvisibleBoogieman47
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Re: contams on mea?? [Re: LocN9ne]
    #23407020 - 07/03/16 12:48 PM (7 years, 6 months ago)

Well that's the first time using the b+ but the other two have no signs of that? I'm going to try the 10/10 mix weetsie said cause all my spores have been growing funny on the way I did it before or I might just do spores on the pda I was doing before they colonized quick and loved it


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Invisibleweetsie
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Re: contams on mea?? [Re: Boogieman47]
    #23407065 - 07/03/16 01:00 PM (7 years, 6 months ago)

Quote:

noob47 said:
So you think the mea is just over powering the spores? It smelled pretty funky in there




Im not sure what that is in the photo, bacteria or yeast but not b+.

A think a weaker agar mix is good for spores, but thats an issue other than your contam problem.


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InvisibleBoogieman47
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Re: contams on mea?? [Re: weetsie]
    #23407075 - 07/03/16 01:05 PM (7 years, 6 months ago)

Haha well of hope it wasn't b+ I probably would never grow them again Lmao ... I'm going to the lake today till tomorrow so when I come back I'm going to try a weaker mea and do some pda like I was doing before and see how it goes if it is a bad syringe I'll have to get ahold of the vendor .. also I've noticed on some transfers I have done if I broke the agar with the blade it did them same thing I just thought it was a sloppy transfer but I know for a fact these spore drops were extra sterile so maybe it is yeast


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Invisibleweetsie
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Re: contams on mea?? [Re: Boogieman47]
    #23407101 - 07/03/16 01:12 PM (7 years, 6 months ago)

try digging a little chunk out of the middle of a dish and put a drop of spores into it then leave the dishes somewhere undisturbed.

Myc can outgrow bacteria on agar but if you have just a tiny bit of liquid on the surface of the agar it can spread the bacteria all over when you move them about.


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InvisibleBoogieman47
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Re: contams on mea?? [Re: weetsie]
    #23407127 - 07/03/16 01:19 PM (7 years, 6 months ago)

Ya I've read that cause some of the ones I didn't use I kept the foil on and kept them in the fridge and had the condensation just stay in there I'm going to have to do a pour instead of being lazy with the no pour


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InvisibleInocuole
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Re: contams on mea?? [Re: Boogieman47]
    #23407220 - 07/03/16 01:46 PM (7 years, 6 months ago)

If condensation is such a huge issue that you can't just dump the water out in the SAB I'm not sure pour agar is going to solve the issue entirely.


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InvisibleBoogieman47
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Re: contams on mea?? [Re: Inocuole]
    #23407235 - 07/03/16 01:52 PM (7 years, 6 months ago)

I wasn't saying it was a huge issue just thought a pour would make it not be as bad but what I was saying is the thing I read on here said once the condensation sat on the mea for a period of time it sometimes made the top look funny ... but the sides of my pasty plates have alot more condensation on them from pc'ing


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InvisibleInocuole
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Re: contams on mea?? [Re: Boogieman47]
    #23407242 - 07/03/16 01:53 PM (7 years, 6 months ago)

I just let them chill for a couple days and they never have condensation.  If they did I would just dump it out.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleBoogieman47
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Re: contams on mea?? [Re: Inocuole]
    #23407253 - 07/03/16 01:56 PM (7 years, 6 months ago)

Ya you're right man good idea thanks


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