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MrSir
Stranger
Registered: 05/18/16
Posts: 18
Last seen: 7 years, 1 month
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Are my jars contaminated?
#23406018 - 07/03/16 03:07 AM (7 years, 6 months ago) |
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Hey I have four jars, each a different type.
P. subaeruginosa, B+, Blue Meanie and Alacabenzi
They have been incubating at 28degrees Celsius for 10 days.
I have got mycelium growth in the P.Sub and B+ jars and I am quite excited but also worried that they may be contaminated. I see a little bit of green (is that mold) in the jars with growth.
The Blue Meanie and Alacabenzi have no growth, does this mean the spores didn't take hold and died?
Please check out the photos and let me know.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Are my jars contaminated? [Re: MrSir]
#23406025 - 07/03/16 03:16 AM (7 years, 6 months ago) |
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Definitely all contaminated, but they also look poorly mixed, looks like mostly verm on top and mostly BRF on the bottom. They all have a very soupy look. What were you following to get jars like that?
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
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Re: Are my jars contaminated? [Re: Inocuole]
#23406073 - 07/03/16 04:44 AM (7 years, 6 months ago) |
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That must stink Why is there rubber gloves on the tops
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ghyphtedhippie
Lord High Sanvalvwag of Hollop

Registered: 06/27/16
Posts: 34
Last seen: 5 years, 7 months
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Re: Are my jars contaminated? [Re: mrmazdarx9]
#23406322 - 07/03/16 08:28 AM (7 years, 6 months ago) |
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They are very badly contaminated far beyond saving. What measurements did you use in preparing those jars? Did you sterilize or pressure cook them? You also may want to consider getting wide mouth jars, you won't be able to get cakes out of those. And I'm a bit curious about the rubber gloves too... what's that about?
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 16 hours
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OMG ... sorry but those are awful...
Please don't open those indoors, or sniff or breathe any of that shit in.
I'm not even sure I wouldn't just bury those jars and all... 
Not trying to be a dick just stating the obvious.
PS - what's with the verm layer?
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
Edited by FreeWorldOrder (07/03/16 12:09 PM)
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Wormi
Quiet Cultivator


Registered: 10/29/14
Posts: 278
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Re: Are my jars contaminated? [Re: MrSir]
#23406576 - 07/03/16 10:20 AM (7 years, 6 months ago) |
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did you pc these? i would guess not. anyway theyre trash. I wouldnt even save the jar.
-------------------- An old man and his grandson are sitting by the fire outside the tepee, wrapped in furs and gazing into the leaping flames. High on a snowy ridge, a wolf howls at the moon and another answers from far away. Soon after, the old man removes the pipe from his mouth. ‘Grandson,’ he says. ‘There are two wolves inside you. One is white and the other is black.’ ‘What are they doing there, Grandfather?’ asks the wide-eyed boy. ‘They are fighting each other,’ says the old man. The boy considers this, then asks, ‘Why are they white and black?’ ‘The white one is your love, your peace and your truth. The black one is your fear, your anger and your lies.’ The fire crackles and sparks flare in the night. The wolf on the ridge howls again and the old man puffs contentedly on his pipe. Finally, the boy says, ‘Which one will win, Grandfather?’ ‘Ah,’ says the old man, removing the pipe once more. ‘The one that wins is the one that you feed.’
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Are my jars contaminated? [Re: Wormi]
#23406633 - 07/03/16 10:42 AM (7 years, 6 months ago) |
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Quote:
FreeWorldOrder said: OMG ... sorry but those are awful...
Please don't open those indoors, or sniff or breathe any of that shit in.
I'm not even sure I wouldn't just bury those jars and all... 
Not trying to be a dick just stating the obvious.

Quote:
Wormi said: did you pc these? i would guess not. anyway theyre trash. I wouldnt even save the jar.
--------------------
Q&A US vs. THEM The more I learn, the less I know.
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Are my jars contaminated? [Re: LocN9ne] 1
#23406961 - 07/03/16 12:25 PM (7 years, 6 months ago) |
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I can't wait for the logic behind this one 
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Are my jars contaminated? [Re: weetsie]
#23407175 - 07/03/16 01:35 PM (7 years, 6 months ago) |
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Funny when you have time to edit the names of everything into pictures in fancy font but haven't yet realized how totally beyond help all those jars are.
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MycoLoopology
Eye's wide open



Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
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Re: Are my jars contaminated? [Re: Inocuole]
#23407361 - 07/03/16 02:27 PM (7 years, 6 months ago) |
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oh god..
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Well its a learning curve my first jars went in the bin my new jars were done under a much stricter sterile environment and up to now its looking good I think the blasting on how bad this looks should stop and move on to make sure it doesn't happen again Best thing about this hobby is you can try again without much financial or time cost Maybe let us know the sequence of events you went through to get to this stage each step in detail
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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MycoLoopology
Eye's wide open



Registered: 06/12/16
Posts: 518
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Re: Are my jars contaminated? [Re: mrmazdarx9]
#23407425 - 07/03/16 02:46 PM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said: Well its a learning curve my first jars went in the bin my new jars were done under a much stricter sterile environment and up to now its looking good I think the blasting on how bad this looks should stop and move on to make sure it doesn't happen again Best thing about this hobby is you can try again without much financial or time cost Maybe let us know the sequence of events you went through to get to this stage each step in detail
might be one of the best posts ive seen from you yet (:
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Quote:
MycoLoopology said:
Quote:
mrmazdarx9 said: Well its a learning curve my first jars went in the bin my new jars were done under a much stricter sterile environment and up to now its looking good I think the blasting on how bad this looks should stop and move on to make sure it doesn't happen again Best thing about this hobby is you can try again without much financial or time cost Maybe let us know the sequence of events you went through to get to this stage each step in detail
might be one of the best posts ive seen from you yet (: 
I'm pretty drunk and eat a lot of codeine
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Are my jars contaminated? [Re: mrmazdarx9]
#23407448 - 07/03/16 02:54 PM (7 years, 6 months ago) |
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 8 hours, 19 minutes
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Re: Are my jars contaminated? [Re: MrSir]
#23407456 - 07/03/16 02:56 PM (7 years, 6 months ago) |
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Holy fuck contaminated lol!
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Are my jars contaminated? [Re: Bigbadwooof]
#23407702 - 07/03/16 04:39 PM (7 years, 6 months ago) |
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but, but, why are you suffocating the jars with gloves??? without GE you'll just fail over and over again.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 8 hours, 19 minutes
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Re: Are my jars contaminated? [Re: dankington] 1
#23407709 - 07/03/16 04:41 PM (7 years, 6 months ago) |
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Sometimes I just wish I could send newbies some home-grown spawn... Fruiting is the fun part anyway! (This is not an offer of shit... to anyone... unless you want edibles... maybe lmao)
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
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Last seen: 1 year, 4 months
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Re: Are my jars contaminated? [Re: Bigbadwooof]
#23407739 - 07/03/16 04:53 PM (7 years, 6 months ago) |
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Quote:
Bigbadwooof said: Sometimes I just wish I could send newbies some home-grown spawn... Fruiting is the fun part anyway! (This is not an offer of shit... to anyone... unless you want edibles... maybe lmao)
Edibles what's the point of eating mushrooms if you don't get a chance to trip and kick god in the teeth lol
Oj I love edibles yum
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 8 hours, 19 minutes
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Re: Are my jars contaminated? [Re: mrmazdarx9]
#23407755 - 07/03/16 04:56 PM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said:
Quote:
Bigbadwooof said: Sometimes I just wish I could send newbies some home-grown spawn... Fruiting is the fun part anyway! (This is not an offer of shit... to anyone... unless you want edibles... maybe lmao)
Edibles what's the point of eating mushrooms if you don't get a chance to trip and kick god in the teeth lol
Oj I love edibles yum
I have to say, I really fucking like that taste of Lions Mane, fresh picked, uncooked. Shiitake are pretty good, and pleurotus (oysters) are alright. They are good for beefing up some fetuccini or something. Reishi have amazing health benefits, and so do shiitake. Although, I think the research on this is a bit sketchy, by Western standards. In Asia they do science a bit differently.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Are my jars contaminated? [Re: Bigbadwooof]
#23407758 - 07/03/16 04:57 PM (7 years, 6 months ago) |
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Those are how I picture jars inoculated with snot would look 
OP you need to pick a tek and follow it exactly. Close don't count for shit in this game, it ain't horseshoes.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 8 hours, 19 minutes
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Re: Are my jars contaminated? [Re: Pastywhyte]
#23408500 - 07/03/16 09:16 PM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: Close don't count for shit in this game, it ain't horseshoes.
Lmao! I'm going to have to save that line!
It's true. Anything short of perfection in your sterile procedure will lead to a nasty mess. Sometimes a small amount of bacteria in a jar can still be used to make a tub, but it's best to avoid that.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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MrSir
Stranger
Registered: 05/18/16
Posts: 18
Last seen: 7 years, 1 month
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Re: Are my jars contaminated? [Re: Bigbadwooof]
#23412597 - 07/05/16 04:03 AM (7 years, 6 months ago) |
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Hey everyone, thanks for the responses.
I followed the steps outlined in this guide
I used gloves to cover the holes in the lids because the foil kept tearing and I though gloves are sterile anyway.
I mixed the brown rice flower and verm as per the ratio in that guide, but double as the guide is for 250ml jars and mine are 500ml.
After adding the verm, water and rice flower into the jars I placed them in the pressure cooker for 45 minutes.
Afterwards I injected the spores with a needle in a sterilised box with gloves on, the needle was heated.
I didn't skip anything, and I see I grew some mycelium so I'm still pretty happy with my first attempt.
Any tips for the next try?
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Are my jars contaminated? [Re: MrSir]
#23412795 - 07/05/16 06:53 AM (7 years, 6 months ago) |
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the foil kept tearing through... are you leaving the foil on your jars after inoculating? that's a no no. your clean box should not have the gloves attached either, Still Air Box > Glove Box.
500ml is not optimal for PF tek cakes, as they're more prone to stalling.
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ghyphtedhippie
Lord High Sanvalvwag of Hollop

Registered: 06/27/16
Posts: 34
Last seen: 5 years, 7 months
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Re: Are my jars contaminated? [Re: MrSir]
#23412816 - 07/05/16 07:04 AM (7 years, 6 months ago) |
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Quote:
MrSir said: Hey everyone, thanks for the responses.
I followed the steps outlined in this guide
I used gloves to cover the holes in the lids because the foil kept tearing and I though gloves are sterile anyway.
I mixed the brown rice flower and verm as per the ratio in that guide, but double as the guide is for 250ml jars and mine are 500ml.
After adding the verm, water and rice flower into the jars I placed them in the pressure cooker for 45 minutes.
Afterwards I injected the spores with a needle in a sterilised box with gloves on, the needle was heated.
I didn't skip anything, and I see I grew some mycelium so I'm still pretty happy with my first attempt.
Any tips for the next try?
After looking at the link you followed, it does not appear as though you followed it. You can see that your jars are not full and the verm on top has become saturated. The purpose of the DRY vermiculite on top it help prevent contamination from making it to the substrate. It is also VERY important to wipe away any excess substrate from the top inch or so of the jar before adding the verm, otherwise you leave a road for contams to travel on.
I'd suggest you acquire the proper 1/2 pint jars before trying again.
Personally, I have never had a properly prepared PF jar go bad on me.
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MrSir
Stranger
Registered: 05/18/16
Posts: 18
Last seen: 7 years, 1 month
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Re: Are my jars contaminated? [Re: dankington]
#23414914 - 07/05/16 07:44 PM (7 years, 6 months ago) |
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Quote:
dankington said: the foil kept tearing through... are you leaving the foil on your jars after inoculating? that's a no no. your clean box should not have the gloves attached either, Still Air Box > Glove Box.
500ml is not optimal for PF tek cakes, as they're more prone to stalling.
Yes, I was lifting the foil, injecting the spores and then when I put the foil back down it was tearing, so I just put a glove over the lid instead.
So the point of the foil is to stop water getting into the jars while inside the pressure cooker? After pressure cooking the jars, I remove the foil, take off the lid, add the dry verm layer, reattach the lid, inject the spores and then cover the holes with surgical tape?
Also I have sourced some wide mouth 250ml jars for the next attempt.
Quote:
ghyphtedhippie said: You can see that your jars are not full and the verm on top has become saturated. The purpose of the DRY vermiculite on top it help prevent contamination from making it to the substrate. It is also VERY important to wipe away any excess substrate from the top inch or so of the jar before adding the verm, otherwise you leave a road for contams to travel on.
I'd suggest you acquire the proper 1/2 pint jars before trying again.
Thanks, now that you mention it I think the mistake I made was not sterilising the dry verm layer, do I do this in the oven?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Are my jars contaminated? [Re: MrSir]
#23415045 - 07/05/16 08:27 PM (7 years, 6 months ago) |
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No. You punch holes in the lid, leave the lid on, sterilize, remove foil, inoculate through the holes in the lid, and leave it the hell alone. You never ever remove the lid after sterilizing unless it's ready for birthing.
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MycoLoopology
Eye's wide open



Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
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Re: Are my jars contaminated? [Re: Pastywhyte]
#23416135 - 07/06/16 06:56 AM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: No. You punch holes in the lid, leave the lid on, sterilize, remove foil, inoculate through the holes in the lid, and leave it the hell alone. You never ever remove the lid after sterilizing unless it's ready for birthing.
just in case it wasnt obvious. this also means you add the verm layer before PC'ing lol.
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
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ghyphtedhippie
Lord High Sanvalvwag of Hollop

Registered: 06/27/16
Posts: 34
Last seen: 5 years, 7 months
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Go to http://www.mushroomvideos.com/ and watch the "BRF/PF Tek" videos, all 4 parts. Pay very close attention, follow the process EXACTLY and you'll succeed. I know it can be a pain to go out and buy something that is almost, but not exactly, what you already have. But, these thing have been chosen by long time experts for the reliability they provide.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 16 hours
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Yes if you follow the TEK to a "T" you will be golden.
It's awfully hard to fuck up a PF BRF grow...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Quote:
FreeWorldOrder said:
It's awfully hard to fuck up a PF BRF grow...
Very true, and yet it still seems to happen every single day for some reason.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Are my jars contaminated? [Re: MrSir]
#23416797 - 07/06/16 12:15 PM (7 years, 6 months ago) |
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Quote:
MrSir said: I didn't skip anything, and I see I grew some mycelium so I'm still pretty happy with my first attempt.
Any tips for the next try?
Im struggling to see any mycelium mate maybe just my untrained eye though
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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