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Invisibledankington
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23400153 - 07/01/16 07:12 AM (7 years, 7 months ago)

Popcorn can be tough to read. Can you find any other, cheaper grains?

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Offlinespacechildo
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Re: My first bulk diary on Shroomery [Re: dankington]
    #23400443 - 07/01/16 09:06 AM (7 years, 7 months ago)

doesnt look good to me, upload the pics here so we can zoom in..

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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: spacechildo]
    #23400508 - 07/01/16 09:40 AM (7 years, 7 months ago)

I usualy use barley grains,they are usually the most available here but i used them all and put them into the unicorn type autoclavable spawn bags tl3 type around 3,5 kilos per each and inoculated them with lizard king and mazatapec,they are pre sealed and thoroughly sterilized so I dont expect any contams in there,spore prints was excellent quality and syringes are made pretty good so that is my main goal,Im just considering about fuiting these with popcorn but only the first two look clean to me and acted clean,they were spawned from agar the rest was g2g and werent inoculated into sab,I currently sterilzing my room and cooking agar to isolate all of the strains to get a pure culture of each,I got 4 pure cultures of malabar finnaly using hydrogen to wash agar after cooking it i soaked it in peroxide and I got that beuatifull pure mycelial growth only bad thing was that the growth was stunned for about a week and then they took off,so that is the best sucess rate I got for now using agar,I am planing on using only the 2 jars that are the most unsuspiciuos looking and smeeling I mean the first 2 they are definitively most clean and most colonized so I doubt that they are contaminated,I just realizeed how much am I mistaken by not doing everything in SAB but intstead risk inoculation in an open environment,from now on I wont fart If its not in a SAB and I mean that,I lost too much time spawning contaminated jars over and over,I could picked mushrooms by now


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I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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Invisibledankington
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23400622 - 07/01/16 10:23 AM (7 years, 7 months ago)

Is that one sentence?
You have all this stuff, just do the agar work properly. You're weakening the myc by pouring peroxide on it. Don't make spore syringes if you're inoculating grains.

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Invisiblemupetmower
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Re: My first bulk diary on Shroomery [Re: dankington]
    #23400704 - 07/01/16 10:42 AM (7 years, 7 months ago)

Quote:

ClintMassey said:
smeeling...,from now on I wont fart If its not in a SAB and I mean that,






Quote:

dankington said:
Is that one sentence?






:lolsy:


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-The wise man never stops seeking knowledge.


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InvisibleInocuole
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Re: My first bulk diary on Shroomery [Re: mupetmower]
    #23401698 - 07/01/16 04:50 PM (7 years, 7 months ago)

Stop soaking shit in peroxide.  Just get rid of the peroxide.  That's for booboos.


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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: Inocuole]
    #23403035 - 07/02/16 03:51 AM (7 years, 7 months ago)

I just tried to do that because I accidentally droped some shit on an agar plate,so I took some hidrogen 3% and just pour it over the agar and mixed it in a stiring motion and poured it out,then put the grains on them and now after 2 weeks I have pure colonies no bacterial or any kind of contamination...call me crazy but the best thing I ever did and the nicest mycelial growth I ever got,I tried before with syringes and spores and transfering and they almost always contaminated mostly all of them contaminated after a while and bacterialy most of the time,white blob started growing over agar,now its pure and only mycelium,I get it that you dont like H202 but it worked for me that time,This time I tried it wihout agar,some g2g transfer and syringe inoculation with a drop of solution on agar so I will see which works better


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I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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InvisibleMunchauzen
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23403219 - 07/02/16 06:38 AM (7 years, 7 months ago)

:saywhat:

Edited by Munchauzen (07/02/16 09:54 AM)

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Offlinespacechildo
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23403571 - 07/02/16 09:38 AM (7 years, 7 months ago)

Quote:

ClintMassey said:
so I took some hidrogen 3% and just pour it over the agar and mixed it in a stiring motion and poured it out,then put the grains on them and now after 2 weeks I have pure colonies no bacterial or any kind of contamination...call me crazy but the best thing I ever did and the nicest mycelial growth I ever got




:callingbullshit:

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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: spacechildo]
    #23405560 - 07/02/16 10:48 PM (7 years, 7 months ago)

Just to say to you guys,I finnaly got pins,I didnt even noticed the prymordia forming and today I looked and the pins are growing out,they are all crystally looking and small but they have reddish caps and there is a lot of them...more that I imagined so I got it now...and Spacechildo I dont know why you make fun of that fact but it is true,I droped something on my agar that would surely contamine it,I took hydrogen 3% and poured some over the agar plate,It didnt contained any inoculans at that time only agar,and swirled it for a bit in a circular motion then just dumped it upside down on a paper towel,than when it drained I cleaned the sides of the jar that was on the paper towel with some antiseptic liquid and inoculated them with grains and now I have nice pure colonies and I will take pictures to prove it,maybe it didnt worked for you but It certainly worked for me,maybe it just soaked the agar on the surface like a film and allowed the grains to develop without any contaminants but I shit you not these are the purest colonies I have on agar right now,I m just waiting to buy a better camera and I will take pictures to prove before I transfer the parts of mycelium that I want on another agar plate so you will get a clean shot because I dont use petri dishes and my lid is not see trough


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I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23405583 - 07/02/16 10:55 PM (7 years, 7 months ago)

but who gives a fuck about agar an H2O2 pins are coming...I feel so relaxed right now,after I dipped the trays in the antibacterial solution the mycelium growth appeared a little different,it became more spider web like you guys get on your casings,I did notice some white balls forming but I didnt recognize that as primordia I thougt that its just mycelium bursting in litle spots here and there but the pins showed up nicely they are now about 1 to 2mm the biggest ones,I gotta go buy a camera fucking today...wuaaaa I just hope that those suckers turn out ok so I cant take some decent prints back


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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Invisibledankington
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23406113 - 07/03/16 05:39 AM (7 years, 7 months ago)

yeah i mean, i'm sure we're all anxious to see what you come up with here.

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InvisibleInocuole
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Re: My first bulk diary on Shroomery [Re: dankington]
    #23407199 - 07/03/16 01:40 PM (7 years, 7 months ago)

Oh, very.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineMycoLoopology
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Re: My first bulk diary on Shroomery [Re: Inocuole]
    #23407317 - 07/03/16 02:16 PM (7 years, 7 months ago)

yeah go buy that camera; and learn the 'enter' button.


--------------------
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Offlinemrmazdarx9
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23407733 - 07/03/16 04:50 PM (7 years, 7 months ago)

Quote:

ClintMassey said:
but who gives a fuck about agar an H2O2 pins are coming...I feel so relaxed right now,after I dipped the trays in the antibacterial solution the mycelium growth appeared a little different,it became more spider web like you guys get on your casings,I did notice some white balls forming but I didnt recognize that as primordia I thougt that its just mycelium bursting in litle spots here and there but the pins showed up nicely they are now about 1 to 2mm the biggest ones,I gotta go buy a camera fucking today...wuaaaa I just hope that those suckers turn out ok so I cant take some decent prints back



And find the right google image so you can't tell I messed it up


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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: mrmazdarx9]
    #23408727 - 07/03/16 10:40 PM (7 years, 7 months ago)

Quote:

mrmazdarx9 said:
Quote:

ClintMassey said:
but who gives a fuck about agar an H2O2 pins are coming...I feel so relaxed right now,after I dipped the trays in the antibacterial solution the mycelium growth appeared a little different,it became more spider web like you guys get on your casings,I did notice some white balls forming but I didnt recognize that as primordia I thougt that its just mycelium bursting in litle spots here and there but the pins showed up nicely they are now about 1 to 2mm the biggest ones,I gotta go buy a camera fucking today...wuaaaa I just hope that those suckers turn out ok so I cant take some decent prints back



And find the right google image so you can't tell I messed it up




You are a funny guy you know,maybe you should try going out to that late night stand up comedy clubs and give it a shot!


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23408789 - 07/03/16 11:03 PM (7 years, 7 months ago)

And here are the pictures with a better camera...oh I mean I had to google it so I can find some decent looking ones "Morron"
Here are my fuck up pins and cakes














Hope you find it very fucked up so you can shit all over the place,as far as I am concerned to much of you people got a very bad ego trip...and you are fucking taking shrooms...what eating a gram or so and dipping your fingertips into water,thanks for all the advice guys but I mean some of you are full of shit and I mean that sincerely,the rest of you thanks for you helpful advices...and yea H2O2 kicks ass and I will continue to use it on every agar plate and any maybe contaminated stuff cause it very much fucking helps,I did once again g2g transfer on agar and didnt used the peroxide wash and guess what bacteria,the ones I did use it on guess what???pure mycelium and a clean colony so fuck that shit as far sa I am concerned the best thing I ever tried for agar preparation,a quick rinse over your agar and no contaminations once you let it dry out a little and go g2g so please do not talk to me about H2O2 never ever again...you proved your point it sucks probably because you dont know how to use it but for me it very much fucking helps,So I hope that cloese that discussion
Peace to you all and good luck


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23408863 - 07/03/16 11:26 PM (7 years, 7 months ago)

And here is the proof that it is wise to rinse your agar surface with H202 and let it uspide dry out a bit then do your transfers without fearing that you will transfer bacteria from a contaminated source or fear contaminations

here is the picture of the ones I transfered with pre-rinsing the agar surface clean and not contaminated


And here is pure transfer from a possibly contaminated source without rinsing the surface of agar...this is 2 or 3 days after the transfer notice the bacteria on the surface,I have some more pictures but I dont feel like taking a picture of every one of them,now its just extra work cooking up some new agar and rinsing it and then transfering from these contaminated plates to the new protected agar plates so I can get pure colonies with a 5 to 6 days more waiting included cause the peroxide needs to be broken apart by the mycelium so it can start spreading on the agar surface!


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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OfflineClintMassey
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23409011 - 07/04/16 12:20 AM (7 years, 7 months ago)

And now when we got all of that shit out of the way,can someone please tell me how much does this pinning lasts,I see more and more pins growing out this is day 2 from the first pins and they are still poking out the surface and on the sides,as you see I pulled out the casing layers and put them on some aluminum foil and made a little wall to protect them from water and touching the bottom of the tub but I revealed more surface for more pins and the pins are poking even on the sides,they are just growing out everywhere,there is a shitload of that little lumps that are pure white and dont have caps and then they turn into pins pretty soon...
I know that not all of them will survive and grow out but I m just curious on aproximatelly how long does the formation of pinheads lasts before they start growing out into mushrooms cause one of my cakes is a little late compared to the other 2 that have a decent ammount of pinheads already?I think its 1 day behind the faster cakes

P.S. "that yellowish liquid on the surface is not contamination its just the antibacterial solution that still comes out of the cakes cause they have been soaked for good amount of time and the color of the antibiotic solution is yellow so when I take a paper towel and gently put it on the yellow liquid it just sucks it out and underneath is white mycelium so I am just saying that for the ones who will come and be like it looks contaminated bla bla bla,its not so please stop with that shit,even if it is I dont give a F... but Its not bacteria its the solution that is still coming out everytime I spray the cakes


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down

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Invisiblewowimflabbergasted
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23409113 - 07/04/16 01:38 AM (7 years, 7 months ago)

Quote:

ClintMassey said:
And here are the pictures with a better camera...oh I mean I had to google it so I can find some decent looking ones "Morron"



lmao that's some funny shit.

Morron - Urban Dictionary: "More commonly, an incorrect spelling of the word 'moron' Used exclusively by true morons in all forms of print as well as the internet. This misspelling is a clear indication that the user is not just a moron but also an illiterate ignoramus incapable of intelligent intercourse with the rest of humanity. :lmafo: I love that site

Just messing with you there OP....but why the fuck are you dipping your subs in peroxide? Maybe the agar...maybe. But substrates? Hmmm................

Fruiting lasts 7-14 days usually.


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