Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Fruiting in jar, colonization concerns
    #23403060 - 07/02/16 04:30 AM (7 years, 6 months ago)

First of all, i'd like to say a big hello to the community and thanks for all the info and advice you people contribute! :smile:

Been sponging up posts for a while now before deciding to start my first mycology experiment. Before I start i'll say that I have used the search function for similar cases but there seemed to be a fair bit of conflict of ideas and processes for the problem. Some clarification would be greatly appreciated.

PICS INCLUDED

PF tek, BRF, Pressure cooker (15psi), TiT incubator (73-75 f), dry verm top layer, 4 weeks growth, P.cubs

So, colonization seemed to be going well and all 8 jars are over 85% (some 95%) surface covered. There seemed to be a halt in growth a 5-6 days ago, then fruits stated appearing.

My concerns from reading are contamination, a short period of time where light got in, FAE, water content of substrate and also jarring procedure of the substrate. Could you add any thoughts ?

Rules followed fairly strictly regarding contam prep. It seems from reading that a little bit of light is fine. Inoculation holes uncovered for FAE. An extra small volume of water was added to the mix to make sure no drying out happened. As far as the substrate in jar goes, it was compressed very lightly. In hindsight maybe not a good idea as further reading has revealed that an almost fluffy consistency cake will allow for better myc growth?

Could somebody also define the difference between a fruit and pin with regards to scale and these pics?

Also, advice on whether to continue colonization, dig a hole, slice parts off, chill them, air exchnge etc etc.
Personally, I was going to wait another while and see what happens

Thanks in advance for the support









Extras: Filter Print Post Top
Offlinemindbentempire
Male


Registered: 10/26/09
Posts: 258
Loc: Australia Flag
Last seen: 17 days, 7 minutes
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23403091 - 07/02/16 04:53 AM (7 years, 6 months ago)

Hi, Welcome to the Shroomery! :smile:

I think you are not clear on what FAE and GE is.
Fresh Air Exchange = you want that during fruiting stage, lots of exchange of air.
Gas Exchange = you want that while colonizing.


Can you tell us why you decided to add more water to your cakes?
And exactly how did you do this? Sterile water / syringe?
This could have contaminated your cakes and made them bacterial.
Hence not 100% colonized and pinning.
From what I know, mycelium colonizes dryer substrates faster, then overly wet ones.

Are there any jars that are 100% colonized?

The ones that are not fully colonized, I would probably just let them grow "invitro"


Extras: Filter Print Post Top
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Re: Fruiting in jar, colonization concerns [Re: mindbentempire]
    #23403104 - 07/02/16 05:06 AM (7 years, 6 months ago)

Thanks for the tip on terminology

the extra water was added with the prep of verm and brf. Bottled water boiled then cooled. Down to inexperience I guess but I had some worries about the jars drying out.
Wont do it again in future

None are 100%. Small patches uncolonized or thinly covered on all jars

Could you link to a reliable invitro tek? i'll research in the meantime


Extras: Filter Print Post Top
Offlinemindbentempire
Male


Registered: 10/26/09
Posts: 258
Loc: Australia Flag
Last seen: 17 days, 7 minutes
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23403172 - 07/02/16 05:54 AM (7 years, 6 months ago)



Extras: Filter Print Post Top
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Re: Fruiting in jar, colonization concerns [Re: mindbentempire]
    #23403284 - 07/02/16 07:19 AM (7 years, 6 months ago)

Thanks again,

Still quite new to alot of the theory so excuse the lack of knowledge here.

Alot of questions for those who are interested, sorry if these have been covered many times

Should I just leave them to fruit in the jars and would this be considered in vitro?

Or should i get these zip lock containers to transfer a cake into each?
I feel that the space surrounding would be beneficial for growth
Should the cakes be cut into layers and non colonized parts removed or just left to colonize/fruit
Is a spacer or verm layer recommended underneath cakes to avoid water pooling when misting?
How does FAE work with in vitro methods? Is the half closed lid enough to provide the right amount of airflow?
Is a casing layer covering the colonized substrate recommended in this situation and would this require crumbling or slicing of cakes?

Maybie most importantly, am I going in the right direction with these questions at all? :p


Extras: Filter Print Post Top
OfflineNDStepp84
Stellar nuclear waste
Male User Gallery

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23403308 - 07/02/16 07:31 AM (7 years, 6 months ago)

Taller jars like that tend to stall, if it was me I would birth them, remove all uncolonized parts and pull off the bigger mushrooms. Dunk for 24 hours, roll in dry verm and place them in a properly constructed sgfc if you want to get the most out of them.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


Extras: Filter Print Post Top
OfflineMycoLoopology
Eye's wide open
 User Gallery


Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
Re: Fruiting in jar, colonization concerns [Re: NDStepp84]
    #23403333 - 07/02/16 07:44 AM (7 years, 6 months ago)

the 4th one just looks like a floppy dick hanging down the inside of your jar :p

thats all i wanted to say. nothing helpful here sorry; i like it tho... :o


--------------------
Current Grow Log

^^My first grow log <3^^

Wish me luck.


Extras: Filter Print Post Top
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Re: Fruiting in jar, colonization concerns [Re: MycoLoopology]
    #23403433 - 07/02/16 08:29 AM (7 years, 6 months ago)

I think i'll keep it simple for first try and go that route NDstep

One question with the sgfc, how do you keep the perlite and moisture from escaping through the bottom holes? Is a raised tray containing the perlite/cakes needed?
Also, does anyone have experience combining a SGFC into a TiT for the extra heat needed? trying to figure out how that would work
Any other workarounds for temp and SGFC? Or is room temp okay usually?

hahaha, mycoloop, just what i was thinking :P


Extras: Filter Print Post Top
OfflineNDStepp84
Stellar nuclear waste
Male User Gallery

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23403486 - 07/02/16 08:56 AM (7 years, 6 months ago)

Room temp is perfect. Look over the basic principles of the sgfc https://www.shroomery.org/forums/showflat.php/Number/20195542#20195542
The sgfc creates air currents flowing in through the bottom holes and up through the perlite. Cakes can sit on squares of foil or jar lids, trays can sit on perlite, or if it is a large tray can be elivated off the perlite. Also when you mist your cakes it adds moisture back to the perlite.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


Extras: Filter Print Post Top
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Re: Fruiting in jar, colonization concerns [Re: NDStepp84]
    #23403577 - 07/02/16 09:39 AM (7 years, 6 months ago)

this answers my questions :smile:

was worried about the perlite flowing through the holes but I realise now the size of the holes and the mechanics going on will stop that from happening

thx for the link

will post an update with pics in a few days

Do you think I should do this step as soon as possible?

Is the only disadvantage to leaving them in the jars longer the uptake of nutrient by shrooms?


Extras: Filter Print Post Top
OfflineNDStepp84
Stellar nuclear waste
Male User Gallery

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23403627 - 07/02/16 10:03 AM (7 years, 6 months ago)

Yeah I'd say. You don't have to be in a major rush but I'd get it done as soon as possible, most important make sure all uncolonized parts are removed :goodluck:


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


Extras: Filter Print Post Top
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Re: Fruiting in jar, colonization concerns [Re: NDStepp84]
    #23403684 - 07/02/16 10:27 AM (7 years, 6 months ago)

Okeeee :laugh:

Thanks :smile:

Flame on the blade then cooled or alcohol?


Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23404217 - 07/02/16 02:08 PM (7 years, 6 months ago)

you wont need to do anything like that. just rinse the uncolonized substrate off under the sink faucet when you rinse right after birth, and right before the 24hour dunk. it should crumble away fairly easily just from rubbing your finger over it. you will be able to tell when its all rinsed away.

listen to the other guys about building a proper SGFC, and fruiting them that way.
just out of curiosity, are those AA+?

also, just from looking at the pics, and with the pinning before 100% in mind, i'd say your cakes are probably slightly bacterial. not the end of the world. just make sure to give them a good smell when you birth them. this will NOT give you 100% certainty of not having contam/bacteria issues, but if it doesnt smell like healthy fresh mushrooms, then you will know to toss it.

i feel like im forgetting something, that i was wanting to say. forgive, me. if i remember i will come back and edit.

good luck :thumbup:


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Extras: Filter Print Post Top
OfflineCyclosphere
Voyager
 User Gallery


Registered: 07/01/16
Posts: 7
Last seen: 6 years, 11 months
Re: Fruiting in jar, colonization concerns [Re: mupetmower]
    #23505936 - 08/03/16 02:25 PM (7 years, 5 months ago)

A while since the last post but a few things happened since last updates.

Let me know what you all think please :smile:







Was wondering about the coloration of some of the fruits? There seems to be some blackish stuff on some of the caps of one cake and underneath this area on the perlite. Im wondering if its some type of mold or other microbe rather than spores? If so what to do?

Also there's one strange looking guy that grew first then almost folded outwards. He looks a little green/bluish. Theory was that this was slightly compressed and malformed from starting to grow in the jar before birthing. Either that or contamination of the fruit.

Thoughts on the pics and progress much appreciated


Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: Fruiting in jar, colonization concerns [Re: Cyclosphere]
    #23506030 - 08/03/16 02:50 PM (7 years, 5 months ago)

black is spores..

caps can get wavy like that when they get really mature. time to harvest.

some do look a little strange color though.. but probably just their pigments.  that mutant on the bottom pic is pretty crazy, too!

oh, just realized you might have meant the mutant when talking about the strange growing fruit. you it's just a mutant. but like i said, colors do look a little off in those pics..


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Extras: Filter Print Post Top
InvisibletheGODSmademedoit

Registered: 03/04/16
Posts: 516
Re: Fruiting in jar, colonization concerns [Re: mupetmower]
    #23506343 - 08/03/16 04:29 PM (7 years, 5 months ago)

The spores are really purple they just look blackish. Thats why the purple hues in there. Really does look pretty with them albinos in the mix nice j o b.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Quart jar colonization - when complete? farmer_maggot 5,635 1 09/12/01 02:48 AM
by NDK
* Re: Will anyone sell me 5 colonized UNCONTAMINATED jars?? (and a little perlite) mycofile 1,557 4 01/31/00 06:58 AM
by Link
* Help: top of jar colonizing sloow FungusFarmer 1,863 3 09/06/01 10:15 AM
by puscle
* Time for jar colonization AbeZard 1,679 5 07/25/01 07:14 PM
by AbeZard
* 1/30 jars colonized in 12 days :o( shroomatomic 1,883 3 03/12/16 02:14 PM
by Leftfield420
* Jar colonize question? Anonymous 1,871 2 03/25/02 03:54 PM
by Anonymous
* Ever have your jars colonize from bottom to top? straightbangin 1,653 2 01/10/02 12:44 PM
by MAIA
* 2 jars colonized without PC... Hell Yeah Raven_420 1,112 2 04/13/02 02:47 PM
by Raven_420

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
610 topic views. 22 members, 157 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 14 queries.