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OfflineRackstrawstiltskin
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Questions about whats happening with my jars
    #23401175 - 07/01/16 01:29 PM (7 years, 6 months ago)

I'm using BRF PF-TEK for B+ and it's been around a month.

I started with 7 jars,
2 468 ml
2 425 ml
3 284 ml half pints

lost 2 due to mold (greenie meanie)

I birthed one a few days ago and got 30-60g yield completely healthy, it's currently dunking. (pint)
I sliced the other into wafers for an ongoing wafer-TEK (pinning) using sterilised verm and BRF to 'case' (I know I should get coir and/or pastuerize but i'm just using what I have.

And the jars I have left are REALLY slowly colonising but with pinning (30%-40%) and I am wondering if there is anything wrong with them.

Cheers for any advice :smile:

TLDR: Please check my pictures and tell me what I should do with these slow colonisers with pins.



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OfflinecronicrFacebook
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23401286 - 07/01/16 02:14 PM (7 years, 6 months ago)

those look bacterial dude


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: cronicr]
    #23401300 - 07/01/16 02:19 PM (7 years, 6 months ago)

The pictures do not show them in a good light, a lot of what might look like bacterial infection is a sticky residue on the outside from a previous wrapper, cheers for the input though, just wondering if the worst case scenario plays out if there is any way to salvage it.

Just because I have seen people say that birthing bacteria infected cakes still yield fruit.


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OfflinecronicrFacebook
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23401391 - 07/01/16 02:53 PM (7 years, 6 months ago)

i'm going to go with"what looks like bacteria..is probably bacteria":thumbup:
not much you can do but wait it out but i wouldnt' hold my breathe, you may get a few from it but it won't be pretty, i'd start more.


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OfflineSporkvirus
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Re: Questions about whats happening with my jars [Re: cronicr]
    #23401421 - 07/01/16 03:03 PM (7 years, 6 months ago)

I suggest working on your sterile procedure.  If you get that down you will have a lot less contamination.


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InvisibleLocN9ne
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23401619 - 07/01/16 04:19 PM (7 years, 6 months ago)

Quote:

Rackstrawstiltskin said:


Just because I have seen people say that birthing bacteria infected cakes still yield fruit.




There are mushrooms growing already man... Birth them motherfuckers...


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: Sporkvirus]
    #23401657 - 07/01/16 04:33 PM (7 years, 6 months ago)

Cheers man, I don't own a PC atm so I used a pot and I guess I must have did it wrong or something, I will use a crock pot instead it seems to trap the moisture in. :smile:


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: LocN9ne]
    #23401669 - 07/01/16 04:38 PM (7 years, 6 months ago)

Some are only around 40-60% with pins, do I just birth them with the colonised amount as is?

I'm just wondering if the moisture from the substrate left because it took so long to colonise and that caused pinning.

The alternative is bacterial infection but I see no huge amount of myc piss (metabolites) fighting off any known infection. They are still colonising every day just very slowly so it seems unlikely if that's the case.

the aerial mycelium looks as if it is getting starved of oxygen, or is that just rhizomorphic growth looking elsewhere for moisture as it's gone from the rest.

Hope RR comes to save the day, I am at a crossroads on whether I should birth them as they are separately (60% colonised max), or keep them as they are and let the pins that are growing go invitro and wait another week for birth?

Cheers for the feedback anyway much appreciated, good vibes :smile:


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InvisibleLocN9ne
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23401688 - 07/01/16 04:45 PM (7 years, 6 months ago)

Well you are gonna be waiting a while for RR to answer your question... Truth is cronicr up there told you exactly what the situation is, like... Without a shadow of a doubt those are bacterial... Contamination will cause early pinning... Its the mushrooms last ditch effort to try and drop some spores in hopes of survival... I would birth them, wash off all of the uncolonized parts and get what you can man.


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: LocN9ne]
    #23401699 - 07/01/16 04:50 PM (7 years, 6 months ago)

Cheers man appreciate the feedback will birth in 3 separate FC's and attempt to salvage what I can. At least I have 2 healthy cakes that will supply some more spores so I can ammend my mistakes from last time. Peace :smile:.


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InvisibleLocN9ne
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin] * 1
    #23401719 - 07/01/16 04:56 PM (7 years, 6 months ago)

I got to ask you a question... What made you accept my advice over someone who has been a member for 5 years, has 50,000 posts, is a trusted cultivator, as well as a mod? I'm just curious because it happens here a lot...


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InvisibleInocuole
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Re: Questions about whats happening with my jars [Re: LocN9ne]
    #23401760 - 07/01/16 05:11 PM (7 years, 6 months ago)

Quote:

LocN9ne said:
I got to ask you a question... What made you accept my advice over someone who has been a member for 5 years, has 50,000 posts, is a trusted cultivator, as well as a mod? I'm just curious because it happens here a lot...




Ohhh now we're getting to the meaty bits.. :popcorn:  Wanna hear this, whatever the reason is.


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Re: Questions about whats happening with my jars [Re: Inocuole]
    #23401804 - 07/01/16 05:31 PM (7 years, 6 months ago)

I'm not taking your advice over his, he says that I should start more but I could get a few out of it (albeit not pretty) so I'm taking both :smile:. I agree it's bacterial and I'm gonna try and see what I can get out of them before I start more.

I guess someone will always go for the hopeful option in any situation.

unless it's mold. Fuck mold. I'm willing to take anyone's advice on this site with experience since I'm totally new to mycology.

Just need to go through the steps of taking a spore print and making it into a syringe. One more question, should I attempt to dunk the cakes or just try to get a flush out of the existing moisture?


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InvisibleLocN9ne
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23401824 - 07/01/16 05:37 PM (7 years, 6 months ago)

:seriousthumbsup:


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Re: Questions about whats happening with my jars [Re: LocN9ne]
    #23403972 - 07/02/16 12:15 PM (7 years, 6 months ago)

If anyone is interested I will be posting any possible fruits I'll get out of the bacterial contaminated cakes and my set up for them for future reference.

Here are them dunking.



You can clearly see the areas on the cakes where it hasn't colonised the substrate fully, still hoping to get maybe 1 flush off each.


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OfflineMycoLoopology
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23404164 - 07/02/16 01:39 PM (7 years, 6 months ago)

early fruiting and uncolonized areas? obvious bacteria. but thats already been said (:

good luck with what youre doing there. i hope you carefully removed as much of the bacterial bit if youre planning to fruit it.


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: MycoLoopology]
    #23404316 - 07/02/16 02:48 PM (7 years, 6 months ago)

Yeah of course, no foul smell and I'll make final cuts after the dunk with a sterilised knife :smile:.


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Invisiblemupetmower
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23404351 - 07/02/16 03:03 PM (7 years, 6 months ago)

that is unnecessary. it wouldnt matter whether the knife is sterilized or not, considering you are doing all this in open air, for one. and secondly, fully colonized cakes are fairly contam resistant, which is why we do not birth/fruit in sterile conditions. thirdly,

you need to do a lot more reading and research. a lot. i cannot stress that enough. start out here.

and, again, good luck :thumbup:


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InvisibleLocN9ne
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Re: Questions about whats happening with my jars [Re: mupetmower]
    #23404720 - 07/02/16 05:32 PM (7 years, 6 months ago)

Should have washed the uncolonized parts off first... Imo


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: LocN9ne]
    #23404751 - 07/02/16 05:43 PM (7 years, 6 months ago)

Yeah I got most of it off, but it gets to a point where it feels like I might be cutting off some of the cake, but idk. I'll see tomorrow when I fruit them :smile:


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Re: Questions about whats happening with my jars [Re: LocN9ne]
    #23422939 - 07/08/16 09:41 AM (7 years, 6 months ago)

Update: they seem to be growing, they look a bit strange but they are growing :tongue2:

Would they be safe to eat is the question... Making a tea might destroy the bacteria? I'm not too sure how to handle this situation.

Here are the pics anyway, tell me what you think :smile:.

PS: I had to smash the glass to get the cakes out (Throwaway jars so it's fine) and it cut some of the pins which probably halted growth (like the blue pin).

But tell me your opinions :smile:.





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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23422956 - 07/08/16 09:46 AM (7 years, 6 months ago)

So you are asking if pins that grew in-vitro are safe to eat? If so, yes they are. Unless they have actual mold growing on them, or they are rotten or something, they are usually safe.


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: mupetmower]
    #23422984 - 07/08/16 09:56 AM (7 years, 6 months ago)

Thanks a lot for taking interest :smile: I appreciate it.

Do you recommend me making a tea? Or have you never done that before?

I'm waiting for my perfectly healthy PFTEK cake to 2nd flush so I can take a spore print and start again.

Heres the first pin of 2nd flush.


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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23423152 - 07/08/16 10:52 AM (7 years, 6 months ago)

Sometimes i make tea but usually I just powder them with my coffee grinder, and then put it in some lemon juice and then drink it.


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OfflineRackstrawstiltskin
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Re: Questions about whats happening with my jars [Re: mupetmower]
    #23423269 - 07/08/16 11:27 AM (7 years, 6 months ago)

Have you tried drying using the oven method? I would grind it up but can't when its fresh.

I don't have any desiccant but I could put the shroom by some rice and fan dry it for a bit and finish off with low heat oven with fans on it?

Appreciate the advice :smile:


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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23423330 - 07/08/16 11:44 AM (7 years, 6 months ago)

Honestly, no I haven't tried any of those things. I just got a cheap food dehydrator, and j wouldn't trust anything else. I know some people do say that oven drying can work, with oven and fan combo(or if the oven has a fan in it) but honestly, food dehydrator can be bought for so cheap that it's almost silly not to get one. They are like $30 for decent ones new.


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-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (07/08/16 01:41 PM)


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Re: Questions about whats happening with my jars [Re: mupetmower]
    #23423454 - 07/08/16 12:22 PM (7 years, 6 months ago)

Pretty tight on money at the moment so I will have to attempt to fan dry with oven/rice.

Thanks a lot for the advice man appreciate it :smile:

Will rate you.


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InvisibleMad Season
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Re: Questions about whats happening with my jars [Re: Rackstrawstiltskin]
    #23423542 - 07/08/16 12:58 PM (7 years, 6 months ago)

Yeah for the first couple years of growing I did fan dry for 24-48 hours on a shoe rack, and then put them in the oven on the lowest heat for 1-2 hours to get them from spongy to cracker dry. I didn't even crack the door when I did it too.


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Re: Questions about whats happening with my jars [Re: Mad Season]
    #23423664 - 07/08/16 01:42 PM (7 years, 6 months ago)

Does your oven maybe have a fan in it mad? Like is the top, possibly? Just curious.


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InvisibleMad Season
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Re: Questions about whats happening with my jars [Re: mupetmower]
    #23423906 - 07/08/16 03:00 PM (7 years, 6 months ago)

Nah just a standard convection oven

:facepalm: conventional not convection.


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Edited by Mad Season (07/08/16 03:06 PM)


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