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InvisiblePastywhyteMDiscord
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Re: Clone test [Re: Boogieman47]
    #23462935 - 07/21/16 03:15 PM (7 years, 6 months ago)

Quote:

noob47 said:
Well we have plenty of rattlesnakes where I live my uncle had about 6 he caught in a cage for about 15 years I wouldn't wanna extract the venom tho haha




It would need to be filtered sterile as well. Lab grade venom is like $800 a gram or something in that range.


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InvisibleBoogieman47
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Re: Clone test [Re: Pastywhyte]
    #23462974 - 07/21/16 03:32 PM (7 years, 6 months ago)

Ya I remember you telling me about that you would think taking a tissue sample would be able to germinate but I guess it would be like sticking a square into a circle hole


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Re: Clone test [Re: Pastywhyte]
    #23462982 - 07/21/16 03:34 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Quote:

noob47 said:
Well we have plenty of rattlesnakes where I live my uncle had about 6 he caught in a cage for about 15 years I wouldn't wanna extract the venom tho haha




It would need to be filtered sterile as well. Lab grade venom is like $800 a gram or something in that range.



Maybe I should look into becoming a rattlesnake farmer goddamn haha jk


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OfflineBigbadwooof
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Re: Clone test [Re: Pastywhyte]
    #23463267 - 07/21/16 05:05 PM (7 years, 6 months ago)

Quote:

noob47 said:
Well we have plenty of rattlesnakes where I live my uncle had about 6 he caught in a cage for about 15 years I wouldn't wanna extract the venom tho haha




I used to catch rattlesnakes when I was a kid. Wish I had some around where I live, I might give it a shot.

Quote:

Pastywhyte said:
Even then it's hit or miss. RR never wanted to talk about the pan cube cross, I suspect for good reason. Even if it did cross, there is no guarantee it would be viable. Horses and donkeys can interbreed to produce mules but the mule offspring is typically sterile.




I wouldn't expect to get viable spores from one of these hybrids. Maybe if they were really closely related. It would be awesome if you could cross P. Cyanescens with Oysters! I'd expect a lot of, if not entirely, funky mutants!


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


Edited by Bigbadwooof (07/21/16 05:06 PM)


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OfflineBigbadwooof
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Re: Clone test [Re: Pastywhyte]
    #23463269 - 07/21/16 05:07 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Quote:

noob47 said:
Well we have plenty of rattlesnakes where I live my uncle had about 6 he caught in a cage for about 15 years I wouldn't wanna extract the venom tho haha




It would need to be filtered sterile as well. Lab grade venom is like $800 a gram or something in that range.




It seems like you could just use wild rattlesnake venom, and then isolate away from contamination after it does it's job.


Anyways, when are we gonna see some Rustywhyte prints?


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


Edited by Bigbadwooof (07/21/16 05:07 PM)


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InvisiblePastywhyteMDiscord
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Re: Clone test [Re: Bigbadwooof]
    #23463320 - 07/21/16 05:24 PM (7 years, 6 months ago)

Gonna be a couple more generations still. . .I don't want a PE6 type situation. They will go out when I am satisfied the lineage is stable enough that it won't be suddenly degraded by people trading poor genetics. I look how quick my previous 4th and then 5th gen came apart and I would be pretty mad at myself if the whole thing came apart in a few short years.

On a better note I did me some harvesting this morning and while some needed to be left to finish up, I am pretty stoked by how beefy these guys are. Pulled some monsters and cut a few in half to find they were totally solid. That is a major plus in my eyes. Pics will be up when I get home later. Stay tuned.


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OfflineBigbadwooof
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Re: Clone test [Re: Pastywhyte]
    #23463332 - 07/21/16 05:26 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Gonna be a couple more generations still. . .I don't want a PE6 type situation. They will go out when I am satisfied the lineage is stable enough that it won't be suddenly degraded by people trading poor genetics. I look how quick my previous 4th and then 5th gen came apart and I would be pretty mad at myself if the whole thing came apart in a few short years.

On a better note I did me some harvesting this morning and while some needed to be left to finish up, I am pretty stoked by how beefy these guys are. Pulled some monsters and cut a few in half to find they were totally solid. That is a major plus in my eyes. Pics will be up when I get home later. Stay tuned.




That's why I want a print! They look really nice and solid. Are the dense like PE, or hollow like AA+? They look dense to me.

What happened with PE6 anyway?


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


Edited by Bigbadwooof (07/21/16 05:27 PM)


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InvisibleBoogieman47
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Re: Clone test [Re: Bigbadwooof]
    #23463356 - 07/21/16 05:33 PM (7 years, 6 months ago)

I'm sure you don't wanna explain the whole break down but what do you mean coming apart ? Like they started leaning towards their own genes?? Like more pe or more aa+ ?


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OfflineHugh Jorgan
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Re: Clone test [Re: Pastywhyte]
    #23463365 - 07/21/16 05:34 PM (7 years, 6 months ago)

Sounds nice. Cant wait to see it stabilized.  Its always nice when you can come up with a nice solid and beefy variety. I've said it before  you da man!!!


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OfflineBigbadwooof
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Re: Clone test [Re: Boogieman47]
    #23463385 - 07/21/16 05:41 PM (7 years, 6 months ago)

Quote:

noob47 said:
I'm sure you don't wanna explain the whole break down but what do you mean coming apart ? Like they started leaning towards their own genes?? Like more pe or more aa+ ?




You have to select for specific alleles repeatedly to get rid of genetics that you don't want to be characteristic of your strain. I'm sure if you didn't do it enough generations, eventually those other genetics would be selected for during cloning, and the strain would no longer be what it was intended to be.

He is trying to shrink the gene pool way down.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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InvisibleBoogieman47
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Re: Clone test [Re: Bigbadwooof]
    #23463424 - 07/21/16 05:52 PM (7 years, 6 months ago)

So the only good way to do that is to keep cloning and collecting spores from the new generation?


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InvisiblePastywhyteMDiscord
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Re: Clone test [Re: Boogieman47]
    #23463453 - 07/21/16 06:00 PM (7 years, 6 months ago)

Cloning is just a way to test attributes that are less easily noticed. Also they are solid throughout. They have a foam in the center that most fruits have hollow. PE and APE often have the same foam. I think it's a trait carried from the CRS tho cause my CRS often do the same.


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Re: Clone test [Re: Pastywhyte]
    #23463480 - 07/21/16 06:08 PM (7 years, 6 months ago)

Ya this batch I've got going of pe is foamy but the first thing I grew of pe they were dense little fuckers I could cut the stems like little logs haha ... OK on stabilizing it would you take a clone of a grow then grow another tub then take spores and so on and so on or you only work with the spores themselves while trying to stabilize it?


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Re: Clone test [Re: Boogieman47]
    #23463498 - 07/21/16 06:11 PM (7 years, 6 months ago)

You do an ms grow then take clones. Test the clones and if one meets the criteria, print it. Then another ms grow and take more clones. Then print the best one and back to ms. Each time you go back to ms it moves forward a generation.

I am speeding things up a bit by only using clones from pins that pop up on agar. Mean I need to expand a lot of plates but takes less time to grow out and usually invitro pins are fairly decent.


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Re: Clone test [Re: Pastywhyte]
    #23463544 - 07/21/16 06:22 PM (7 years, 6 months ago)

Ya that's what I was thinking so how do you know when it is ready to be stable enough I mean I know it doesn't start dancing for you but what shows that you stabilized it right ?


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OfflineBigbadwooof
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Re: Clone test [Re: Pastywhyte]
    #23463629 - 07/21/16 06:54 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Cloning is just a way to test attributes that are less easily noticed. Also they are solid throughout. They have a foam in the center that most fruits have hollow. PE and APE often have the same foam. I think it's a trait carried from the CRS tho cause my CRS often do the same.




When you say foam, you mean it's solid, or is it the type of texture that when it is dry it still feels spongy? I get that sometimes with PE.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


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InvisiblePastywhyteMDiscord
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Re: Clone test [Re: Bigbadwooof]
    #23463719 - 07/21/16 07:22 PM (7 years, 6 months ago)

It's like PE. Feels spongy when dry.


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Re: Clone test [Re: Pastywhyte]
    #23463728 - 07/21/16 07:25 PM (7 years, 6 months ago)

The ones I have growing now they are no where near as dense as my last what would cause that? Maybe my sub too dry?


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OfflineBigbadwooof
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Re: Clone test [Re: Boogieman47]
    #23463732 - 07/21/16 07:26 PM (7 years, 6 months ago)

Quote:

noob47 said:
The ones I have growing now they are no where near as dense as my last what would cause that? Maybe my sub too dry?




Genetics.

Whenever I get Penis Envy that with the tiny stems that look like they are wearing helmets... They are dense as fuck...


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy


Edited by Bigbadwooof (07/21/16 07:27 PM)


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InvisibleBoogieman47
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Re: Clone test [Re: Bigbadwooof]
    #23463740 - 07/21/16 07:31 PM (7 years, 6 months ago)

I'm going to post on the threads my pe grow maybe some of you can look to see what's up with it I'm not trying to hijack your thread pasty sorry


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