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Offlineskunkfl0wer
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creating a contamination resistant mushroom
    #23396758 - 06/30/16 08:13 AM (7 years, 6 months ago)

so i have both a question and an idea based on the experiences i'm about to share.

it all started when i wanted to grow some shrooms, i have grown them before but only a little and i wanted to stock up. so i ordered 6 syringes from that one site that will give you a free syringe for every one you buy. i got my jars together and because the syringes looked quite weak i put all 72 cc's in 32 seperate 1 pint jars. now that i look at it, that was probably a bad idea in the first place but i found out, as time went on, that i used to much water in the brf mixture which we all know spawns contamination. jars were continuously being pulled from the shelf until there were only a few. i was still kind of happy until another contamination came in and destroyed all but 1. 1 is better than nothing so i birthed it, got my mushies, and was able to fill 6 more syringes while i was at it. woohoo.

So i try again, i make the jars and to be cautious before i inoculate all of them i test each syringe on two jars for a total of 12 jars. now this was my first time making them so i had no clue what i was doing. the first syringe i made i was using very bad methods, no flame sterilization or steam, only rubing alchahol which pretty much guarenteed me contamination. untill i found out about the sucking up boiling water trick and steam cleaning things for the other 5. for some reason i didn't even think that it was probably contaminated and tested it anyway.

well within the span of 2 weeks those jars began growing beautiful in every hole and getting bigger every day until it was about 3/4 mycelium in which contamination finaly showed. 1 spot in one jar and 2 spots in another both directly below the inoculation holes. by that time i had already expected it and wasn't surprised but i deicided to save them in another room anyway. i come back 1 week later and all contamination is gone. the one jar that had 2 contams i can see a very small discoloration still left because i know where it is but if i didn't i might not even recognize it. the mycelium seems to be destroying it and not even showing it's stress hormone or whatever it is when it starts producing the yellow liquid. i'm pretty sure it's going to completely eat it and it makes me wonder... have i in some way created this resistance in the mycelium by breeding it and can it be bred further to possibly allow growing contamination-less without pressure cooking.

i'm not sure how common this is which is why i ask your thoughts on this and if you think it's safe to eat these mushrooms.


Edited by skunkfl0wer (06/30/16 11:51 AM)


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Offlinejsncrs
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Re: creating a contamination resistant mushroom [Re: skunkfl0wer]
    #23396780 - 06/30/16 08:28 AM (7 years, 6 months ago)

It's pretty common for mycelium to defeat contams, depending on genetics, the strain you're using, what type of contamination it is, the ratio of bacteria to mycelium and how early the mycelium gets a hold on it.

Based on my own personal knowledge, I don't think breeding a contamination resistant strain is possible. The mycelium and bacteria battle it out over the nutrient source (grains) in your jar, and once mycelium fully colonizes a jar it becomes very resistant to contamination.

So a contamination resistant strain isn't the answer, what you'd need to do is somehow create a nutrient source that is resistant to certain bacteria/organisms.


Edited by jsncrs (06/30/16 08:41 AM)


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Offlinedrake89
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Re: creating a contamination resistant mushroom [Re: jsncrs]
    #23397497 - 06/30/16 01:16 PM (7 years, 6 months ago)

Quote:

jsncrs said:
It's pretty common for mycelium to defeat contams, depending on genetics, the strain you're using, what type of contamination it is, the ratio of bacteria to mycelium and how early the mycelium gets a hold on it.

Based on my own personal knowledge, I don't think breeding a contamination resistant strain is possible. The mycelium and bacteria battle it out over the nutrient source (grains) in your jar, and once mycelium fully colonizes a jar it becomes very resistant to contamination.

So a contamination resistant strain isn't the answer, what you'd need to do is somehow create a nutrient source that is resistant to certain bacteria/organisms.



It's called spawn mate


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OfflineInner knowing
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how do post [Re: drake89]
    #23397792 - 06/30/16 02:48 PM (7 years, 6 months ago)

Hey sorry to do this on your post mate but I'm brand new and can't find anywhere how to post my own post/thread. Can anyone help me? Sorry to do this on your post lol.


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Offlineskunkfl0wer
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Re: how do post [Re: Inner knowing]
    #23397834 - 06/30/16 03:02 PM (7 years, 6 months ago)

hmm, i figured that continuously breeding from mycelium that fought off contamination would build a genetic resistance but if noone knows for sure then maybe i will have to personally experiement with that idea myself.


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Offlinejsncrs
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Re: how do post [Re: skunkfl0wer]
    #23398546 - 06/30/16 06:41 PM (7 years, 6 months ago)

I know it's called spawn :yesnod:
Is spawn not a nutritious material that feeds mycelium?


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Invisiblewoodrow
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Re: how do post [Re: skunkfl0wer]
    #23399847 - 07/01/16 02:54 AM (7 years, 6 months ago)

Quote:

skunkfl0wer said:
hmm, i figured that continuously breeding from mycelium that fought off contamination would build a genetic resistance but if noone knows for sure then maybe i will have to personally experiement with that idea myself.



I suspect the spores we get from vendors are adapted to sterile laboratory conditions where they grow well but they have lost their natural resistances. It would be nearly impossible to breed for genetic resistance to contams by growing shrooms on contaminated grains unless the old genes for resistance are switched off rather than entirely gone. 

Wild shrooms are naturally contam resistant. They have to be to grow on cow patties but they are scavengers that normally don't grow on nutrient rich media such as grains or even fresh cow plop. Other microbes tear into anything with readily available nutrients and, then when the first invaders starve out, the shrooms come in and clean up what is left including the dead invaders so shrooms normally only grow on nutrient poor materials where not much else can survive. That is their ecological niche.

We have the choice of either growing shrooms on nutrient poor media and expect slow growth and poor yields but good resistance or grow shrooms on nutrient rich media and deal with contams.


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Invisible36fuckin5
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Re: how do post [Re: woodrow]
    #23413242 - 07/05/16 10:15 AM (7 years, 6 months ago)

Wild mushrooms aren't contam resistant. There's just room enough outside for everything. You're not gonna breed a contam-resistant strain. It simply doesn't work like that. Mushrooms are never going to eat contams, which is what that would take.


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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineLennybernadino
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Re: how do post [Re: 36fuckin5]
    #23414326 - 07/05/16 04:41 PM (7 years, 6 months ago)

Mushrooms are known to have substrate memory and probably a memory for fighting certain contams, thay are also known to exchange genetic information with other mushrooms of the same species, i always thought that maybe one could teach young mycelium by combining it with older experienced wiser mycelium of perhaps the same genetic strain and maybe the memory of one can be transfered to a young colony.


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OfflinePussyFart
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Re: how do post [Re: Lennybernadino]
    #23414576 - 07/05/16 05:55 PM (7 years, 6 months ago)

I think creating a contamination resistant substrate would yield better results, considering the substrate is what the contams colonize, not mycellium.

But then again good sterile technique is really all that is needed to be contam free.....

Just my opinion.


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