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mrmazdarx9
Pffffttt

Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 7 months
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My teas are basically a slightly stronger coffee but i have had strong effects from chewing alot of leaves both cheeks full and chewed and sucked until there was nothing but flavourless gunk then swallowed it.
Quote:
Bigbadwooof said: Yeah, maybe my little baby novos that are on the verge of death (already *tear*) have 10% alkyloid content!
If they are stressed they do produce more alkaloids at the cost of shortened life span so who knows lol
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ferrel_human
stone eater



Registered: 06/26/09
Posts: 16,590
Loc: Mexico
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Re: Coca growers (moved) [Re: mrmazdarx9]
#24534074 - 08/06/17 04:23 AM (6 years, 9 months ago) |
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I thinks its 1% percent alkaloids per leaf no? I read that somewhere. Maybe Dbotany.
-------------------- Nature is my church and walking through it is gospel. It tells no lies and reveals all to those who look, and listen, closely. -Karode

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mrmazdarx9
Pffffttt

Registered: 05/15/16
Posts: 9,796
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Is that stressed or happy plants? I doubt mine are that strong.
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P. semilanceata
Milker of knowledge

Registered: 07/29/17
Posts: 189
Last seen: 3 years, 14 days
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Hi all Erythroxylum growers! I have been interested in tissue culture for long time and have read one book about it called "Plants from test tubes". Does any one have access to this paper? I wonder if tissue culture is fastest way to create Erythroxylum alkaloids. I was meant to try tissue culture of Erythroxylum, C. sativa, T. bridgesii and L. williamsii but I never got the equipment for it and wanted to first learn agar and g2g so that I can make sure my sterile technique is working. Now I have started to learn agar and I am going to do g2g after I have isolated clean culture. After about two months if my sterile technique is working I will order some seeds and supplies for tissue culture and will start my work.
Have anyone else thought or maybe even tried tissue culturing Erythroxylum?
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mrmazdarx9
Pffffttt

Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 7 months
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Very interesting i may have a go myself Seems simple enough. Nice little read shame some of the pics dont work, thanks for the idea.
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DualWieldRake
Stranger


Registered: 07/17/16
Posts: 1,115
Loc: Zone 8b
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Quote:
P. semilanceata said: Hi all Erythroxylum growers! I have been interested in tissue culture for long time and have read one book about it called "Plants from test tubes". Does any one have access to this paper? I wonder if tissue culture is fastest way to create Erythroxylum alkaloids. I was meant to try tissue culture of Erythroxylum, C. sativa, T. bridgesii and L. williamsii but I never got the equipment for it and wanted to first learn agar and g2g so that I can make sure my sterile technique is working. Now I have started to learn agar and I am going to do g2g after I have isolated clean culture. After about two months if my sterile technique is working I will order some seeds and supplies for tissue culture and will start my work.
Have anyone else thought or maybe even tried tissue culturing Erythroxylum?
Yes, you do. www.sci-hub.io
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Chemical Addiction



Registered: 08/16/11
Posts: 2,020
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Log in to view attachment
Quote:
P. semilanceata said: Hi all Erythroxylum growers! I have been interested in tissue culture for long time and have read one book about it called "Plants from test tubes". Does any one have access to this paper? I wonder if tissue culture is fastest way to create Erythroxylum alkaloids. I was meant to try tissue culture of Erythroxylum, C. sativa, T. bridgesii and L. williamsii but I never got the equipment for it and wanted to first learn agar and g2g so that I can make sure my sterile technique is working. Now I have started to learn agar and I am going to do g2g after I have isolated clean culture. After about two months if my sterile technique is working I will order some seeds and supplies for tissue culture and will start my work.
Have anyone else thought or maybe even tried tissue culturing Erythroxylum?
Here's the paper, thanks for the link DualWieldrake
-------------------- Vegetation has crawled for miles towards the cities. It is waiting. Once the city is dead, the vegetation will cover it, will climb over the stones, grip them, search them, make them burst with its long black pincers; it will blind the holes and let its green paws hang over everything. —Jean-Paul Sartre, Nausea
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P. semilanceata
Milker of knowledge

Registered: 07/29/17
Posts: 189
Last seen: 3 years, 14 days
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Thanks a ton DualWieldRake for that link! Just what I needed as I have hundreds of scientific papers bookmarked as "to read" and I did not have access to them. Thank you!
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Bigbadwooof
Snitterbundem The Dirty



Registered: 12/07/13
Posts: 14,480
Last seen: 10 hours, 26 minutes
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Great post! Thanks. I think I will also save mine for that purpose. I just started some khat plants too. In a few years, I intend to make a quid/liquor infusion of 3 strains of khat, coca leaves, and an indian ephedrine plant I have. Should be an invigorating experience. I'd love a shot of that booze! Lol
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "The Party told you to reject the evidence of your eyes and ears. It was their final, most essential command." - George Orwell Every one of you should see this video. "Facts are chiels that winna ding, and downa be disputed" - Robert Burns
 
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Bigbadwooof
Snitterbundem The Dirty



Registered: 12/07/13
Posts: 14,480
Last seen: 10 hours, 26 minutes
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Novogranatense leaves have he highest concentration of alkyloids, approaching 2%. They produce less leaf mass than coca though, but have better flavor, therefore, in south america novos are called 'chewers coca'.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "The Party told you to reject the evidence of your eyes and ears. It was their final, most essential command." - George Orwell Every one of you should see this video. "Facts are chiels that winna ding, and downa be disputed" - Robert Burns
 
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Bigbadwooof
Snitterbundem The Dirty



Registered: 12/07/13
Posts: 14,480
Last seen: 10 hours, 26 minutes
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Quote:
P. semilanceata said: Hi all Erythroxylum growers! I have been interested in tissue culture for long time and have read one book about it called "Plants from test tubes". Does any one have access to this paper? I wonder if tissue culture is fastest way to create Erythroxylum alkaloids. I was meant to try tissue culture of Erythroxylum, C. sativa, T. bridgesii and L. williamsii but I never got the equipment for it and wanted to first learn agar and g2g so that I can make sure my sterile technique is working. Now I have started to learn agar and I am going to do g2g after I have isolated clean culture. After about two months if my sterile technique is working I will order some seeds and supplies for tissue culture and will start my work.
Have anyone else thought or maybe even tried tissue culturing Erythroxylum?
I have considered doing this, but I dont believe there is an established protocol for erythroxylum novogranatense.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "The Party told you to reject the evidence of your eyes and ears. It was their final, most essential command." - George Orwell Every one of you should see this video. "Facts are chiels that winna ding, and downa be disputed" - Robert Burns
 
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Alpineswift
Strangler


Registered: 10/14/16
Posts: 220
Last seen: 1 year, 9 months
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First sprout
But I have a feeling the seed isn't supposed to be leaning like that at all,should I support the top so it points straight upwards?
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Bigbadwooof
Snitterbundem The Dirty



Registered: 12/07/13
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Last seen: 10 hours, 26 minutes
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Quote:
Alpineswift said:

First sprout
But I have a feeling the seed isn't supposed to be leaning like that at all,should I support the top so it points straight upwards?
No, thats fine. It will lift itself from the soil... wtf is it planted in though? Lol
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "The Party told you to reject the evidence of your eyes and ears. It was their final, most essential command." - George Orwell Every one of you should see this video. "Facts are chiels that winna ding, and downa be disputed" - Robert Burns
 
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Alpineswift
Strangler


Registered: 10/14/16
Posts: 220
Last seen: 1 year, 9 months
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Well this one fell through the sphangnum moss and sprouted into the peat moss ,I lifted up top layer to snap the pic
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mrmazdarx9
Pffffttt

Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 7 months
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 Just one is all i want fingers toes and testicles crossed
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Bigbadwooof
Snitterbundem The Dirty



Registered: 12/07/13
Posts: 14,480
Last seen: 10 hours, 26 minutes
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Quote:
mrmazdarx9 said:
 Just one is all i want fingers toes and testicles crossed
You're not crossing your testicles hard enough. It should hurt.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "The Party told you to reject the evidence of your eyes and ears. It was their final, most essential command." - George Orwell Every one of you should see this video. "Facts are chiels that winna ding, and downa be disputed" - Robert Burns
 
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mrmazdarx9
Pffffttt

Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 7 months
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Lol it feels like a kick in the nuts each time the flowers drop off
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 9 months, 5 days
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Back from vacation.. they look well taken care of! new set of leaves
I think the left one made a cripple first set of leaves due to my scalpel being a little off the mark a while ago.

So alpine, you haven't actually planted that germinating berry yet? Cause I wouldn't use much sphagnum in my planting soil mix personally and definitely not chunks, maybe very small shreds and just a little bit which I do have. Holds water like crazy, which is great for germination but I think it would attract problems later on.
My venus fly trap though I have planted in sphagnum as an experiment (well it's boggy AF so not a weird substrate for venus fly trap) and it's doing alright... But sphagnum is weird, you can hardly kill it because it will just propagate asexually when enough water is present and grows like grass which yes I have to mow with the venus fly trap lol!
Don't worry about the seedling reeling with it's delicate parts stuck in the seed husk. It will be top heavy for a while and has to rodeo that unit off.. Which mine didn't manage for the most part, maybe they need to be in the America's for that.
As for the ephedrine / cathinone stuff.. I'd personally add some theanine to that tincture to take the edge off cause those cathinones don't really seem like clean stims, ephedrine is not nice for your heart. Fortunately I doubt the amounts of cocaine involved would make that a complication.. Theanine is cool! It can be subtle but also attenuates the downsides of stims like caffeine. You will not get an active dose from drinking tea though. Realistically such a tincture should be benign compared to pure synthetic stims cf. cocaine and coca tea... I've dabbled with synthetic cathinones including ephedrine and find them highly suspect and know they metabolize into nasty shit resembling heart medications.
Will look up the tissue culture details!! interesting
Is there a way to prep your coca's or novo's to have them produce alkaloids despite not standing at the foot of an Andes mountain or something like that?
Edited by Solipsis (08/09/17 09:14 AM)
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SalviaGod
Bad Person, Good Guy



Registered: 05/07/12
Posts: 747
Last seen: 11 days, 8 hours
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Re: Coca growers [Re: Solipsis]
#24542736 - 08/09/17 10:17 PM (6 years, 9 months ago) |
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Is distilled white vinegar a safe ph down to use for these plants?
When you ph down are you wanting the water you water with to be 5-5.5ph or do you want your run off water to be 5-5.5ph?
I also saw farther back in the thread that people are using 9-3-6 npk liquid ferts, but whats the ratio of ferilizer to water? And how often do you add fertilizer, every time you water? This is all assuming i would be growing in coco coir and perlit as a non nutritious substrate.
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 9 months, 5 days
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AFAIK you want both to be acidic, if the soil is acidic like that and you water with low pH water too then it should just stay the way it is. Watering with pure non-acidic water would leech acids and bring pH back to ~7 over time, watering with a pH even lower than the soil brings down the soil's pH.
So: first check if your soil's pH needs to be perhaps a bit lower, then if it needs to be lower, water with a bit extra acid (pH is a logarithmic scale though so I wouldn't go crazy low). If it is fine just water with that same sort of pH.
Vinegar should be absolutely fine I think, it's all about the concentrations (and thus pH). Maybe the odd acid here and there might chelate other compounds and leech them, or might contain toxic elements... but not your regular organic ones.
idk about ferts they are not all that necessary for me just yet.
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