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compost
Stranger
Registered: 07/15/13
Posts: 18
Last seen: 7 years, 6 months
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Cloning question; what do you do with the colonized agar petri dishes?
#23394377 - 06/29/16 02:07 PM (7 years, 6 months ago) |
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Hello again, so I'm going to be trying cloning for the first time in a few days and wanted to clarify a few things before I screw it all up.
This is probably a stupid quesion, but I've gone through various cloning guides and they all end right at the colonization of the petri dishes and then just say "and then transfer to grain" or something.
How exactly does one do this? Once the petri dish has been colonized, is it like a mini cake? Do I just open up the dish and and dump the contents in a jar of grain? Won't opening the grain jar post pressure cooking defeat the purpose of sterilizing it?
Or is it a liquid that I'm supposed to extract with an empty syringe, and then inoculate the jars the same way as when I first bought syringes?
Also I saw some concern about "old myc", and I got to wondering, if I'm cloning by taking tissue samples, and that tissue sampls is reverting to mycelium and colonizing whatever it's in, is it essentially immortal? Once that little chunk of tissue has gone through the whole process all over again and the new batch is fruiting, can you do the same process with the new fruit, or does it have an age limit?
Any help would be appreciated, thanks
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning question; what do you do with the colonized agar petri dishes? [Re: compost]
#23394416 - 06/29/16 02:17 PM (7 years, 6 months ago) |
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https://www.shroomery.org/forums/showflat.php/Number/20048771
There's links to videos on how to do all of this ifnyou click through the links in my signature
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Cloning question; what do you do with the colonized agar petri dishes? [Re: bodhisatta]
#23394820 - 06/29/16 04:31 PM (7 years, 6 months ago) |
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You use a sterile scalpel to cut a wedge of agar from the plate and drop it into a grain jar, working in a sterile area is more important than with a syringe for the reasons you stated.
Cloning does not reset the clock. Cloning multiple generations after another will cause senescence at some point and once it happens it's game over for that strain unless you have some younger mycelium, (e.g the agar plate from your first clone).
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning question; what do you do with the colonized agar petri dishes? [Re: weetsie]
#23394829 - 06/29/16 04:34 PM (7 years, 6 months ago) |
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Senescence is a rare problem for people doing what we do. If you're a good enough cultivator you can avoid. If you're not chances are you couldn't juggle a single culture that long anyway
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