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PutACapInHisAss
Stranger Than Fiction



Registered: 08/11/07
Posts: 252
Loc: Earth
Last seen: 6 years, 4 months
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Thoughts/Questions About A2A Transfers
#23394039 - 06/29/16 12:05 PM (7 years, 6 months ago) |
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I've been reading about agar for a while. I've watched the videos by Munchauzen.
My thought is, if you are doing multiple transfers from one plate, why not just slice a grid on it with a flamed scalpel, and then use a flamed pair of tweezers to quickly move pieces to the new plates.
Do people tend to not do multiple transfers from one plate? Or, is there some other problem with this strategy?
I'm gonna try this today and see what happens. Wish me luck. 
Thanks to Munchie, Pasty and all the others who've posted about their agar work!
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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i mean, no i guess you can do this, and people do make multiple transfers..
but the thing is, that you usually take the transfer from the outer edge of the growth, to get the strangest myc. also, using multiple tools will just give more possible contam vectors.
also, when you are transferring from a plate, sometimes you will be wanting to transfer away from contam, which if your just making a grid, you will likely transfer the contam out with it. not to mention, running your knife along the entirety of the plate gives higher change to contaminate, as well. you might run your blade through a patch of contm(or even hidden contam) and then continue running it through the rest of the plates, infecting the rest with said contam.
hope that makes sense. im a little scattered right this second. someone else can probably explain it better than i can.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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I get at least 4 transfers from each plate and all 4 are successful most of the time. But fuck tweezers. I tried using flames tweezers for dropping pins onto agar plates to make clones...turned out terrible. Then I just used the inside tissue f fully grown shrooms with my scalpel and it turned out beautiful. Scalpels are so much easier to keep everything contaminant free.
Be sure to flame between each transfer though.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Thoughts/Questions About A2A Transfers [Re: Kush_Zombie]
#23394110 - 06/29/16 12:23 PM (7 years, 6 months ago) |
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In fact, I still have the plates I took transfers from and even after 4 transfers they all just look white and normal...except with squares cut out. And this was a week or 2 ago
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Thoughts/Questions About A2A Transfers [Re: mupetmower] 1
#23394126 - 06/29/16 12:28 PM (7 years, 6 months ago) |
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I agree with mupet here, and you also want to transfer only a tiny piece of myc. If you're doing agar -> grain you can do what you said, to better distribute and still use all the myc on the plate. (once you've transfered enough to know its clean)
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Thoughts/Questions About A2A Transfers [Re: Kush_Zombie] 1
#23394127 - 06/29/16 12:28 PM (7 years, 6 months ago) |
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heh, i keep most the plates i transfer from, also. i let them go until they start pinning, so i can get a pin clone =]
i was thinking about using flamed tweezers, as well, but then reazlied how much easier it was to just stab it with the knife(of even cut out the spot where it grew) and put it on the recieving plate, that way.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Thoughts/Questions About A2A Transfers [Re: mupetmower]
#23394201 - 06/29/16 12:55 PM (7 years, 6 months ago) |
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Yeah dude, I love seeing pins form on my agar. I just like to see what it'll do and I also like to see if any contaminations form on it from taking multiple transfers
but I get about a 90% success rate if not more while doing all my work in a SAB. Granted I do a couple of transfers after taking it from the tissue->agar but I think a transfer or two is best regardless of what you're doing.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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PutACapInHisAss
Stranger Than Fiction



Registered: 08/11/07
Posts: 252
Loc: Earth
Last seen: 6 years, 4 months
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Re: Thoughts/Questions About A2A Transfers [Re: mupetmower]
#23394920 - 06/29/16 05:05 PM (7 years, 6 months ago) |
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Even scattered, the logic of this is quite clear. Thanks for spelling it out for me.
Isolation of contact on the plate before transfer is clearly very important. My plates look super clean but who knows what I am not seeing!
Quote:
mupetmower said: but the thing is, that you usually take the transfer from the outer edge of the growth, to get the strangest myc. also, using multiple tools will just give more possible contam vectors.
also, when you are transferring from a plate, sometimes you will be wanting to transfer away from contam, which if your just making a grid, you will likely transfer the contam out with it. not to mention, running your knife along the entirety of the plate gives higher change to contaminate, as well. you might run your blade through a patch of contm(or even hidden contam) and then continue running it through the rest of the plates, infecting the rest with said contam.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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well, im glad it made at least some sense. 
good luck!
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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