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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393298 - 06/29/16 07:36 AM (7 years, 6 months ago) |
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I've had clean cultures look like that when the mycelium has a hard time staying above the surface. The thicker growth is where the mycelium finally manages to break the surface. It is still a good idea to make transfers before using that plate as an inoculant though, having a uniform plate brings more peace of mind than a patched plate like that. Doesn't mean it's dirty, just means don't trust it just yet.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393303 - 06/29/16 07:38 AM (7 years, 6 months ago) |
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Quote:
Supalemonhaze said: I've had clean cultures look like that when the mycelium has a hard time staying above the surface. The thicker growth is where the mycelium finally manages to break the surface.
say what? surface of the agar? why would myc have a hard time on agar? I've never had growth like this turn out clean, its separating because its meeting an enemy.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393304 - 06/29/16 07:39 AM (7 years, 6 months ago) |
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heh great analogy.
and yeah, i didnt mean to use it as inoculant, but just to transfer from it, still, to see if it can be saved. but, i guess if its only a second transfer from MS, and you have more spores, then maybe it would be easier to just start over.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393322 - 06/29/16 07:47 AM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
Supalemonhaze said: I've had clean cultures look like that when the mycelium has a hard time staying above the surface. The thicker growth is where the mycelium finally manages to break the surface.
say what? surface of the agar? why would myc have a hard time on agar?
Beats me, always only happened to me when I was using GWA though so I'm guessing too much nutes or something. Once I transferred back on LMEA it grew normally. Meshed in mold usually grows in "patches" IME but I don't have much experience with those.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393338 - 06/29/16 07:55 AM (7 years, 6 months ago) |
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but what do you mean when you say break the surface? as if the myc was growing on the underside of the agar trying to break through it?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393345 - 06/29/16 07:58 AM (7 years, 6 months ago) |
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Yeah, it would seem like it's growing under the surface. You can still see the mycelium above the surface if you use a light but it doesn't grow thick until it manages to surface properly. Once it does, it always looked like the first two pics in the OP, where light growth turns into thich growth and then settles down for the rest of the plate. When this happened to me, I could see the agar being pushed up where the mycelium is trying to colonize through it.
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spacechildo
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393354 - 06/29/16 08:02 AM (7 years, 6 months ago) |
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you're talking about a hot pour or sandwich tek right? you havent held a light up to OPs plates? I'm really confused now 
I think OP said this was a transfer or 2 away from original plate in his other thread..
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393358 - 06/29/16 08:04 AM (7 years, 6 months ago) |
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i actually have two clone plates that seemed to have done the same thing. they had very thin, wispy looking growth, that even seemed to be below the surface, and then all the sudden, it started growing from the top, and got thick, and healthier looking. but with these, thee growth did it in a perfect circle, and not in weird lines like OP's.
I mean, im sure it couldve easily been bacteria or mold that caused this to happen, im still too new to properly identify what is actually going on with it. but i transferred from it, so we will see what happens.
Oh, and yeah, space, OP said that these plates are second transfer from original i think.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393362 - 06/29/16 08:06 AM (7 years, 6 months ago) |
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No lol. I'm talking about regular transfers made on GWA which appeared very similiar to OP's and turned out to be clean.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393370 - 06/29/16 08:11 AM (7 years, 6 months ago) |
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This is what i was talking about:

thats a plate made from a tissue sample from an Ecuadorian fruit that grew in another plate.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393378 - 06/29/16 08:15 AM (7 years, 6 months ago) |
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Quote:
Supalemonhaze said: No lol. I'm talking about regular transfers made on GWA which appeared very similiar to OP's and turned out to be clean.
so the myc didnt colonize the top of the agar, but rather dived into the agar, grew underneath the surface and then surfaced to the top again further away on the plate?
never seen anything like it and having problems picturing how or why that would happen. too much nutes just makes my myc look like a lazy chubby.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393402 - 06/29/16 08:27 AM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
Supalemonhaze said: No lol. I'm talking about regular transfers made on GWA which appeared very similiar to OP's and turned out to be clean.
so the myc didnt colonize the top of the agar, but rather dived into the agar, grew underneath the surface and then surfaced to the top again further away on the plate?
never seen anything like it and having problems picturing how or why that would happen. too much nutes just makes my myc look like a lazy chubby.
Not dive in per se, just colonizes the top very weakly, if you don't shine a light at an angle you couldn't see it at all. It's like it colonizes just below the surface with random linear (organized) mycelium poking through.
Then about half an inch of growth later, it surfaces hard, growing very thick and white. The following colonization is not as dense as the "surfacing" but noticeably denser than when it was growing under the surface.
To be honest, I have no idea if it was because of an overly nutritious agar recipe, it's just the only thing that varied. All my PDA and GWA batches seem to be overly-nutritious, mycelium always grows very wispy on them but I only experienced this phenomona randomly during about a 2month time span and only with the same GWA recipe.
I have been diluting my GWA with a 1:3 ratio lately, mycelium still grows wispy AF but at least it grows above the surface. I use MEA mostly, it's my favourite for thick organized growth. Never had the same results with PDA or GWA.
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mupetmower
Mower of Muppets



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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393408 - 06/29/16 08:29 AM (7 years, 6 months ago) |
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i need to get some MEA, so i can start varying recipes for the myc. plus, i think i have been making my PDA wrong. either too nutritious or too stiff or something. im not sure if this is the case, but i just have a feeling ive been doing something wrong, for some reason hah..
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: mupetmower]
#23393413 - 06/29/16 08:32 AM (7 years, 6 months ago) |
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PDA is my least favourite recipe. Apart from growing only very thin and wispy mycelium, the mix is clouded by the potato flakes floating around. GWA is very clear but growth is typically the same, very hard to tell if it's organized or not and impossible to tell sectors apart. If it wasn't for MEA, I would be having a much harder time with agar.
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Spiritwithin
Humidifier


Registered: 05/08/16
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Loc: Germany
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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393434 - 06/29/16 08:46 AM (7 years, 6 months ago) |
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Hey... it's all above the survace I tell you. Well, I'll transfer it anyways and keep you guys updated. Seems to be of interest, lol.
I still don't think it's mold... except for picture 3, that could all be mold. I used PDY-agar. These potato-flakes don't dissolve fully, so maybe the nutrients in the plates aren't very evenly distributed...
SW
-------------------- Trading Golden teacher prints for Pan. cyan. prints.
Edited by Spiritwithin (06/29/16 08:48 AM)
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spacechildo
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Re: Look at my plates... what do you see? [Re: mupetmower]
#23393447 - 06/29/16 08:52 AM (7 years, 6 months ago) |
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sediments dont bother me other than for visibility, I like all agar recipes basically  if you get real whispy growth try not diluting your gwa supa. Frank added SITR or PDA to his undiluted GWA to get it as he likes it IIRC.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: Spiritwithin]
#23393451 - 06/29/16 08:53 AM (7 years, 6 months ago) |
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The mix will still have dissolved flakes in it, there is nutritioun in every part of the plate. I don't think plate number 3 is mold, I think it's just wispy.
Your culture is still very young, the wierd growth could just be because there is a lot of strains growing on top of each other. Still, you should refrain from using a plate like that as an inoculant until you are completely sure it's clean. We use agar to better our chances for success, it would defeat that purpose if you were to inoculate jars with suspect mycelium.
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Spiritwithin
Humidifier


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Re: Look at my plates... what do you see? [Re: Supalemonhaze]
#23393509 - 06/29/16 09:12 AM (7 years, 6 months ago) |
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Na... I'm not planning to inoculate anything with these plates yet. I'm just curious and it's fun for me to do this. Creating some skills too...
I have some fully colonized jars from MS sitting there waiting for their horse manure... The plates are just a side project, since I don't see the point of cloning, but that's another subject.
I have way too much time on my hands, which is good and bad.
Thank you all for your contributions!
-------------------- Trading Golden teacher prints for Pan. cyan. prints.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393510 - 06/29/16 09:12 AM (7 years, 6 months ago) |
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Quote:
spacechildo said: sediments dont bother me other than for visibility, I like all agar recipes basically  if you get real whispy growth try not diluting your gwa supa. Frank added SITR or PDA to his undiluted GWA to get it as he likes it IIRC.
I would think that not diluting would only make it worse. I have seen a slight improvement by changing a 1:2 ratio to a 1:3 ratio (GW:water). I guess I could try doing a small pour without diluting, just to see. A lack of nutrients should give you thicker mycelium strands though, so I dunno how that would work.
This is what I typically get with GWA. You can't see it very clearly but notice how it's impossible to tell if growth is organized or not, even witha light shining through the back. The mycelium is so thin that the light is just making it super bright.
 
While this is typical growth that I get with a lower nute MEA mix. (1g per 100ml) You can easily see the growth on this one, even without a light through the back. With the light, the growth is thick enough for it to not be shiny.
 
I will try diluting it less though, everything is worth trying at this point. I only change media every month or two, even then, I only keep it on the new recipe for only 1 transfer. I am expecting trouble if I keep doing this in the future.
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HybridprX
Biodegrader of coir



Registered: 01/29/08
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Re: Look at my plates... what do you see? [Re: spacechildo]
#23393524 - 06/29/16 09:17 AM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
mupetmower said: like you could still take what looks like it's growing ok and transfer and clean it up, right?
nothing looks like its growing OK to me, its all got mold mixed in. maybe thats where our confusion came from.
water is 1/3 oxygen but breathing under water is still pretty impossible since you cant single out the oxygen molecules (or cube myc in this scenario) out of the water (contams)
Im at work sweating my ass off and I just took the time out of my day to tell you to stop talking out of your ass....there is no mold on ops plates.
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