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OfflineSpiritwithin
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Registered: 05/08/16
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Look at my plates... what do you see?
    #23391276 - 06/28/16 02:36 PM (7 years, 6 months ago)

Fellow mycologists,

I've done some agar work... these are all second transfers from GT MS.
Now I see some sectoring in the inner part (1st and 2nd picture) but should I not take samples from the outer rim for my next transfer?

I'm pretty positive that this is not mold, but I  could be wrong of course...

Any advice would be appreciated.

Thx



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Trading Golden teacher prints for Pan. cyan. prints.


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OfflineHybridprX
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Re: Look at my plates... what do you see? [Re: Spiritwithin]
    #23391294 - 06/28/16 02:41 PM (7 years, 6 months ago)

Take them from the thickest/fastest outer growth. I can see a few sectors worthy of transfer.


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OfflineKenetic
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Re: Look at my plates... what do you see? [Re: Spiritwithin]
    #23391305 - 06/28/16 02:44 PM (7 years, 6 months ago)

Ha I see the fairest maiden of them all....in about 6 or 7 transfers....:smile:

Edit: looks like your plates are a litte overrun.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Edited by Kenetic (06/28/16 02:46 PM)


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OfflineSpiritwithin
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Re: Look at my plates... what do you see? [Re: HybridprX]
    #23391308 - 06/28/16 02:46 PM (7 years, 6 months ago)

Quote:

HybridprX said:
Take them from the thickest/fastest outer growth. I can see a few sectors worthy of transfer.




Thanks for answering. I want to make three new plates... Would you be so kind as to tell me which sectors you would choose?
Maybe in a "2nd picture 8 o'clock" way...
I would very much appreciate it!


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OfflineSpiritwithin
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Re: Look at my plates... what do you see? [Re: Kenetic]
    #23391331 - 06/28/16 03:01 PM (7 years, 6 months ago)

Quote:

kenetic said:
Ha I see the fairest maiden of them all....in about 6 or 7 transfers....:smile:

Edit: looks like your plates are a litte overrun.




Tomorrow I will transfer to new plates :wink:... If I figure out from where to take the samples until then. Maybe it won't make such a big difference...
I think when the temperature droped quite a bit a few days ago, the mycelium suddenly grew thicker. Could that be?


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OfflineKenetic
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Re: Look at my plates... what do you see? [Re: Spiritwithin]
    #23391361 - 06/28/16 03:19 PM (7 years, 6 months ago)

Your best bet it to take several samples from different, similarly aggressive growth areas and branch off from there, simply because you seem to be trying to isolate a random strain from multispore.  Dont just get one isolate becase it could be anything.  You are gonna need a lot of plates (40?) To isolate a few strains and then you need to test each one seperately. 
But to answer your immediate question I would try the first pic at 8 (am or pm, your choice), first pic at 10, and second pic at noon, for starters.
Next time try isolating a clone, you have way less genetics to work with.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleSupalemonhaze
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Re: Look at my plates... what do you see? [Re: Kenetic]
    #23391369 - 06/28/16 03:24 PM (7 years, 6 months ago)

Isolating a clone will still give you a clone. Getting a true isolate is a fun and exciting process. For me anyway.


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OfflineSpiritwithin
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Re: Look at my plates... what do you see? [Re: Kenetic]
    #23391407 - 06/28/16 03:39 PM (7 years, 6 months ago)

Quote:

kenetic said:
Your best bet it to take several samples from different, similarly aggressive growth areas and branch off from there, simply because you seem to be trying to isolate a random strain from multispore.  Dont just get one isolate becase it could be anything.  You are gonna need a lot of plates (40?) To isolate a few strains and then you need to test each one seperately. 
But to answer your immediate question I would try the first pic at 8 (am or pm, your choice), first pic at 10, and second pic at noon, for starters.
Next time try isolating a clone, you have way less genetics to work with.




Ok. Thank you very much!

Now about the clone: I thought about that, but, If I clone 1 mushroom I cannot test its potency since 1 shroom is way to less weight to trip. So even though it may look nice and healthy its still a matter of sheer luck, is it not?


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OfflineKenetic
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Re: Look at my plates... what do you see? [Re: Spiritwithin]
    #23391467 - 06/28/16 04:03 PM (7 years, 6 months ago)

No thats why you test them.  Grow the isolates out pf style or something and look for desirable traits.
You dont have to eat just one shroom test it out.  Measure out a gram or something.
Im too lazy for all of this.  I just get a clone tissue sample and grow it out on a half inch of rye and g2g. 
In your case just keep using agar, but try it sometime with a clone.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisibledankington
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Re: Look at my plates... what do you see? [Re: Kenetic]
    #23391531 - 06/28/16 04:24 PM (7 years, 6 months ago)

third plate sure looks pretty nice to me!


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InvisibleSupalemonhaze
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Re: Look at my plates... what do you see? [Re: dankington]
    #23392788 - 06/29/16 12:34 AM (7 years, 6 months ago)

Really? I think I'd prefer to with 1 and 2. Don't like wispy cultures all that much.


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Offlinespacechildo
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Re: Look at my plates... what do you see? [Re: Spiritwithin]
    #23393232 - 06/29/16 07:02 AM (7 years, 6 months ago)

still looks fucked to me :shrug:

making a new thread doesnt change the fact, maybe it can give you the answer you're looking for tho :goodluck:


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Invisiblemupetmower
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Re: Look at my plates... what do you see? [Re: spacechildo]
    #23393246 - 06/29/16 07:11 AM (7 years, 6 months ago)

im fairly new to agar, and super new to isolating sectors and what not, but if it were me, i would take from plate 1 at exact 6o'clock, where the myc sorta juts out a tiny bit. i would also try to get right around 1:45 and 12:30, where they jut out.

and for plate 2 i would try to get right around 3:00-3:15, and then around 11:00 and 2. for all those, its where you can see it jut out a little, and sorta looks stronger.

as for plate 3, idk haha..

again, im super new to this, but thats probably what i would choose.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinespacechildo
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Re: Look at my plates... what do you see? [Re: mupetmower]
    #23393263 - 06/29/16 07:19 AM (7 years, 6 months ago)

just look at all the separating lines, definitely not clean


cube myc and cube myc grow together, cube myc and foreign contam myc separates like this.


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Invisiblemupetmower
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Re: Look at my plates... what do you see? [Re: spacechildo]
    #23393269 - 06/29/16 07:21 AM (7 years, 6 months ago)

it is still salvageable, right? idk haha. like you could still take what looks like it's growing ok and transfer and clean it up, right?


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinespacechildo
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Re: Look at my plates... what do you see? [Re: mupetmower]
    #23393275 - 06/29/16 07:24 AM (7 years, 6 months ago)

and hope mold isnt intertwined in your transfer? sure.
but why hope when you can start over and do it right?

Starting off a grow from clearly contamed plates isn't something I'd recommend.


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Invisiblemupetmower
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Re: Look at my plates... what do you see? [Re: spacechildo]
    #23393281 - 06/29/16 07:26 AM (7 years, 6 months ago)

oh. i just thought that was something that people did regularly? taking transfers from clearly contam'd plates and cleaning it up with multiple transfers, to be able to use.

Not being sarcastic or anything, i just really thought that was one of the main purposes of using agar.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinespacechildo
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Re: Look at my plates... what do you see? [Re: mupetmower]
    #23393287 - 06/29/16 07:30 AM (7 years, 6 months ago)

yeah, but when the mold is intertwined in the myc how do you separate them?
if you get some satelites or mold growing on 1 side just transfer away. I doubt OP will be able to get the mold away from his myc since its all mingled together.


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Invisiblemupetmower
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Re: Look at my plates... what do you see? [Re: spacechildo]
    #23393290 - 06/29/16 07:30 AM (7 years, 6 months ago)

ohh.. hmm.. ive still got a lot to learn, then.

so, in this case, you just toss the first two plates and transfer from 3?


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (06/29/16 07:31 AM)


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Offlinespacechildo
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Re: Look at my plates... what do you see? [Re: mupetmower]
    #23393295 - 06/29/16 07:33 AM (7 years, 6 months ago)

Quote:

mupetmower said:
like you could still take what looks like it's growing ok and transfer and clean it up, right?




nothing looks like its growing OK to me, its all got mold mixed in.
maybe thats where our confusion came from.

water is 1/3 oxygen but breathing under water is still pretty impossible since you cant single out the oxygen molecules (or cube myc in this scenario) out of the water (contams)


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