|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: Never not starting from agar again... [Re: LadysKnight]
#23386065 - 06/26/16 11:03 PM (7 years, 7 months ago) |
|
|
All I'm saying is.. All I've gotten so far on my agar is a variety of contaminants and no myc, while I've got some mycelium growing in my grain jars (albeit slowly as hell). Don't get me wrong, I'm not saying fuck agar; Imma keep pumping out new plates every couple days till I get some good growth, but damn. Y'all make it seem so easy!! I think I am going to attempt to transfer a colonized grain onto agar soon as well as test my sterile tech with blank plates.
--------------------
Highwalker
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|

The last thing to show up on this plate was cube myc, which was covered in bacteria at that point. I was able to salvage it, just took multiple transfers away from bacteria. Just because you see contams on a plate doesn't mean it's insta-trash.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Never not starting from agar again... [Re: Mad Season]
#23386164 - 06/26/16 11:51 PM (7 years, 7 months ago) |
|
|
Quote:
Mad Season said:

The last thing to show up on this plate was cube myc, which was covered in bacteria at that point. I was able to salvage it, just took multiple transfers away from bacteria. Just because you see contams on a plate doesn't mean it's insta-trash.
Well said. Cleaning up cultures is the best part of agar.
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: Never not starting from agar again... [Re: Mad Season]
#23386165 - 06/26/16 11:52 PM (7 years, 7 months ago) |
|
|
Ooh good to know! Thank you Mad. I assumed it would be too late for the myc to take foot with the other contams present. I thought it was like a transfer it fast enough before contams grow or they're done for. I have a pile of plates in a separate room waiting to be cleaned out so tomorrow I'll see if there's any myc that ended up germinating in the contamd plates before cleaning em.
--------------------
Highwalker
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
|
Side note, if I were to open a nasty plate in an SAB to do a transfer, wouldn't the SAB be filled with many more nasty spores than normal so I would want to just do the single transfer, then take down my set up and clean it out. Rather than doing a lot of transfers at once.
--------------------
Highwalker
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
The spores get stuck to the wet walls of the SAB. If you do your transfer smooth it's really not an issue.
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: Never not starting from agar again... [Re: Pastywhyte]
#23386180 - 06/26/16 11:59 PM (7 years, 7 months ago) |
|
|
Alrighty thanks Pasty. Perhaps I need to do that insence trick to check how much I disturb the air while I'm working or something. Practice makes perfect.
--------------------
Highwalker
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
|
How long would you guys say is sufficient time to see if cube mycelium is going to germinate or if the contam will just keep growing? A week?
--------------------
Highwalker
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Never not starting from agar again... [Re: Pastywhyte]
#23386191 - 06/27/16 12:06 AM (7 years, 7 months ago) |
|
|
Quote:
Pastywhyte said:
Quote:
Mad Season said:

The last thing to show up on this plate was cube myc, which was covered in bacteria at that point. I was able to salvage it, just took multiple transfers away from bacteria. Just because you see contams on a plate doesn't mean it's insta-trash.
Well said. Cleaning up cultures is the best part of agar.
For real! Honestly I've never had a culture I couldn't clean up. The only times I couldn't get a healthy clean culture was when I get stubborn spores that don't germinate 
This is the reason I really don't get why people still argue against agar. I mean in the end I really don't care, but it just makes me think they just haven't done it yet, or done it right. Once you do it right, you're like, "so I'm doing an extra couple weeks for 100% clean inoculant? Sold."
@luke idk really depends on the contam. That plate had the myc germinate a good 2 weeks after the aspergillus germinated (which I almost transferred)
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 10 hours
|
Re: Never not starting from agar again... [Re: Mad Season]
#23386883 - 06/27/16 07:49 AM (7 years, 7 months ago) |
|
|
Yeah just keep at it.....when I was first starting out with agar I kept on getting this:

But I seriously just started making about four new plates a day for a couple weeks and did transfers and spore inoculations just to practice my sterile technique....and before I knew it my colonizing closet looked like this:

But the most specially cool part about it is that in just about two-three months of introduction and practical application of agar I had managed to isolate my very own PE strain....pictures in my signature.....so seriously....stick with this....it's the best way.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: Never not starting from agar again... [Re: Pastywhyte]
#23387025 - 06/27/16 08:42 AM (7 years, 7 months ago) |
|
|
Quote:
Pastywhyte said:
Quote:
FriedEgg said:
Quote:
Pastywhyte said: MS is fine. But start it on agar. I have had more ms than I care too in order to move my RW lineage forward. Still had some good results with it tho.
These are ms

when is that RW going to be ready? 
Well I will probably spawn the 4th gen clones Tues or Wednesday. I was thinking of taking it one or two more gen before starting to get em out.
Hey Pasty, those RW in your Journal entry of your favorite grows are awesome. what exactly are they? you have a thread or something about them somewhere?
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
MycoLoopology
Eye's wide open



Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
|
Re: Never not starting from agar again... [Re: Mycologist217]
#23387143 - 06/27/16 09:27 AM (7 years, 7 months ago) |
|
|
Quote:
Mycologist217 said: Yeah just keep at it.....when I was first starting out with agar I kept on getting this:

But I seriously just started making about four new plates a day for a couple weeks and did transfers and spore inoculations just to practice my sterile technique....and before I knew it my colonizing closet looked like this:

But the most specially cool part about it is that in just about two-three months of introduction and practical application of agar I had managed to isolate my very own PE strain....pictures in my signature.....so seriously....stick with this....it's the best way.
i was one for 3 on my first agar :/ im hoping for good results this time around though. would you recommend grain to agar over MS to agar? and just out of curiosity, what about grain to LC?
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
|
DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
|
Re: Never not starting from agar again... [Re: MycoLoopology]
#23387164 - 06/27/16 09:38 AM (7 years, 7 months ago) |
|
|
Wow. I'm slow. I wasn't even thinking grain to agar. Even though I've done it before. Wow. Sometimes my brain just don't work right. I just nocced up a bunch of agar plates with what I had left in my vendor ms syringes. However I did also have contam ed jars with a bunch of healthy myc. Damn. I could got things going a lot quicker. I can't believe I forgot to do that. Anywho, I'm finna do that tommorow now.
--------------------
Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Never not starting from agar again... [Re: mupetmower]
#23387204 - 06/27/16 09:56 AM (7 years, 7 months ago) |
|
|
Quote:
mupetmower said:
Quote:
Pastywhyte said:
Quote:
FriedEgg said:
Quote:
Pastywhyte said: MS is fine. But start it on agar. I have had more ms than I care too in order to move my RW lineage forward. Still had some good results with it tho.
These are ms

when is that RW going to be ready? 
Well I will probably spawn the 4th gen clones Tues or Wednesday. I was thinking of taking it one or two more gen before starting to get em out.
Hey Pasty, those RW in your Journal entry of your favorite grows are awesome. what exactly are they? you have a thread or something about them somewhere?
Here is the grow log. Is still ongoing.
https://www.shroomery.org/forums/showflat.php/Number/20228521
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
|
Quote:
lukehighwalker710 said: Ooh good to know! Thank you Mad. I assumed it would be too late for the myc to take foot with the other contams present. I thought it was like a transfer it fast enough before contams grow or they're done for. I have a pile of plates in a separate room waiting to be cleaned out so tomorrow I'll see if there's any myc that ended up germinating in the contamd plates before cleaning em.
Pasty, Mad, anybody else in this thread: would you mind looking at this plate from my trash pile? Not sure if it's a contam or myc https://www.shroomery.org/forums/showflat.php/Number/23387773
--------------------
Highwalker
|
MycoLoopology
Eye's wide open



Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
|
Re: Never not starting from agar again... [Re: DeadPhan]
#23390179 - 06/28/16 07:12 AM (7 years, 6 months ago) |
|
|
Quote:
DeadPhan said: Wow. I'm slow. I wasn't even thinking grain to agar. Even though I've done it before. Wow. Sometimes my brain just don't work right. I just nocced up a bunch of agar plates with what I had left in my vendor ms syringes. However I did also have contam ed jars with a bunch of healthy myc. Damn. I could got things going a lot quicker. I can't believe I forgot to do that. Anywho, I'm finna do that tommorow now.
lol thats why having a community of minds always helps. glad i could be of some use :p
i cant wait to get mine going. thanks to mycologist207s(?) recommendation. im going to try grain > agar, agar > LC, agar/LC > Grain Bags/Jars
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
|
|