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OfflineDeadPhan
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Never not starting from agar again...
    #23384038 - 06/26/16 12:34 PM (7 years, 7 months ago)

There's really not much of a viable excuse for doing anything but. Aside from just being impatient and too eager. I've already lost about half the summer already. I'm still coming up with contams.  The most recent was a seemingly healthy master to a bunch of newly altered plastic lids with sfds as I decided to invest and switch from metal rings and lids to plastic and from polyfil to sfd. Well, I'm hoping there was a latent unseen contam in the master. I've got a couple more g2gs from other masters so well see. I'm so exhausted from chasing and trying to pinpoint source of contams. When, if I just always start from agar I'll be able to pinpoint so much better. For a while I been back and forth with wondering if my flowhood is faulty, or possibly fault of filters or or from inoculation source. I honestly hope it's just the inoculation and doing MS straight to grain. Master jars seemingly healthy, but possibly harboring an unseen contam.

I just did a bunch of agar about a week ago. 20 no pour jars.  12 being nocced with a variety of 4 different types from ms, and 3 of those jars left out in front of the hood with the cover off as a control experiment. The remainder in fridge for transfers. I'd done this before in the past and found flowhood was just fine. Hope so again. If I find flowhood is and fault I'll go back to SAB for time being until I invest in making another hood. My worry on my hood is its been stored in some compromising places over the past 3 or 4 yrs I've had it. Some old basements, dirty drafty garages, carports, vehicles, etc. Anyway I get 100 percent success more often than not. But once I get a bad run going it just seems to snowball. Especially when working with Ms. I've had great runs with MS but at the end of the day, it's not worth the wondering. It's too hard to know for sure that a batch got contam ed by, faulty technique, spawn, fh issues, filter issues. From agar at least I'll know where in the weak links are. If any at all aside from inoculate source.


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Big Gulps!  Alright!  Well,  See ya later!
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InvisiblePastywhyteMDiscord
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Re: Never not starting from agar again... [Re: DeadPhan]
    #23384085 - 06/26/16 12:51 PM (7 years, 7 months ago)

Yep. People might see 70% success with spores to grain. This might sound good to some people. But let's say you run 4 tubs a month, which really isn't that much. That's 48 tubs a year. Which means that taking 30% losses you will see 14-15 failed moldy messes a year. Who in the hell wants to clean up that mess? Not me.

Lets say you have a bad streak (syringe) and see half of those losses back to back. That's 7-8 tubs and means two straight months of fail. That would be discouraging to a vet. For a noob it could make them give up.

Now there are other factors besides inoculate that can contribute. But they become easier to pinpoint and narrow down when you know your culture is clean.


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Invisiblemaddchef
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Re: Never not starting from agar again... [Re: Pastywhyte]
    #23384106 - 06/26/16 01:02 PM (7 years, 7 months ago)

Heh try failing for years on end about 50% of the time and being too damned hardheaded and or stupid to figure it out.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineLeftfield420
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Re: Never not starting from agar again... [Re: Pastywhyte]
    #23384118 - 06/26/16 01:09 PM (7 years, 7 months ago)

It only took me losing 2 tubs to start agar work....now I don't see me going back to ms for anything at all...unless I'm putting a drop on a couple plates and passing it along to the next man in need in the marketplace


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InvisiblePastywhyteMDiscord
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Re: Never not starting from agar again... [Re: Leftfield420]
    #23384126 - 06/26/16 01:14 PM (7 years, 7 months ago)

MS is fine. But start it on agar. I have had more ms than I care too in order to move my RW lineage forward. Still had some good results with it tho.

These are ms



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OfflineLeftfield420
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Re: Never not starting from agar again... [Re: Pastywhyte]
    #23384298 - 06/26/16 02:22 PM (7 years, 7 months ago)

Damn...those look fuckin awesome....but I don't have my shit down yet.....still learning, fixing to put together a 66qt mono of TOC  or pink buffalo in the next few weeks hopefully? You know how it goes trying to plan shit out as a noob...so far shit hasn't gone quite tge way I wanted....but I'm still here


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Invisiblemaddchef
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Re: Never not starting from agar again... [Re: Leftfield420]
    #23384306 - 06/26/16 02:24 PM (7 years, 7 months ago)

man makes plans and god laughs


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineLeftfield420
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Re: Never not starting from agar again... [Re: maddchef]
    #23384321 - 06/26/16 02:30 PM (7 years, 7 months ago)

Exactly now I just roll with it


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OfflineMycologist217
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Re: Never not starting from agar again... [Re: Leftfield420]
    #23384324 - 06/26/16 02:31 PM (7 years, 7 months ago)

If you need guidance planning out a MONOTUBS grow, I encourage you to browse through the grow logs linked in my signature, I write my logs in very nice to follow format, and start them by laying out my learning objectives, supplies, and time table! Latest logs include literature on perpetuating cultures with agar and narrowing down your work to your very own isolate strain!


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My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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InvisibleFriedEggS
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Re: Never not starting from agar again... [Re: Pastywhyte]
    #23384698 - 06/26/16 05:03 PM (7 years, 7 months ago)

Quote:

Pastywhyte said:
MS is fine. But start it on agar. I have had more ms than I care too in order to move my RW lineage forward. Still had some good results with it tho.

These are ms





when is that RW going to be ready? :tongue:


--------------------

(Yes, the egg is real)

How to post pics


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InvisibleInocuole
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Re: Never not starting from agar again... [Re: maddchef]
    #23384727 - 06/26/16 05:10 PM (7 years, 7 months ago)

Quote:

maddchef said:
Heh try failing for years on end about 50% of the time and being too damned hardheaded and or stupid to figure it out.




235.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineHybridprX
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Re: Never not starting from agar again... [Re: FriedEgg]
    #23384739 - 06/26/16 05:15 PM (7 years, 7 months ago)

Always innoc a test jar prior to mass inoculation when using multi spore.... it's common sense....

Never shake a grain jar after innoc....you will be able to spot the contamination upon germination (if present) if you shake then you can miss a hidden contamination....


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InvisibleInocuole
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Re: Never not starting from agar again... [Re: HybridprX]
    #23384743 - 06/26/16 05:16 PM (7 years, 7 months ago)

Unlike with a LC that doesn't necessarily guarantee the cleanliness of the rest of the syringe. You could just have 1-3 CFUs(colony forming units) of bacteria in there that don't grow and don't want to break up and end up in different jars.  That's not really enough to fuck a grow necessarily but that's not the point.  That's different with LC because if there were ever 1-3 CFUs of bacteria, there would be hundreds or thousands by the time you used it anyway.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisiblePastywhyteMDiscord
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Re: Never not starting from agar again... [Re: FriedEgg]
    #23384817 - 06/26/16 05:37 PM (7 years, 7 months ago)

Quote:

FriedEgg said:
Quote:

Pastywhyte said:
MS is fine. But start it on agar. I have had more ms than I care too in order to move my RW lineage forward. Still had some good results with it tho.

These are ms





when is that RW going to be ready? :tongue:




Well I will probably spawn the 4th gen clones Tues or Wednesday. I was thinking of taking it one or two more gen before starting to get em out.


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OfflineLeftfield420
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Re: Never not starting from agar again... [Re: Pastywhyte]
    #23384881 - 06/26/16 05:54 PM (7 years, 7 months ago)

That's cool as hell man....one day I hope to understand how to create my own variety....I'm sure it will be years down the line


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Invisiblemaddchef
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Re: Never not starting from agar again... [Re: Inocuole]
    #23385001 - 06/26/16 06:23 PM (7 years, 7 months ago)

Quote:

Inocuole said:
Quote:

maddchef said:
Heh try failing for years on end about 50% of the time and being too damned hardheaded and or stupid to figure it out.




235.





Homo, this isn't a main thread lol back to 234


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleInocuole
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Re: Never not starting from agar again... [Re: maddchef]
    #23385022 - 06/26/16 06:29 PM (7 years, 7 months ago)

Results 1 - 25 of 235

I didn't even wanna reply but... you wrong son.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisiblemaddchef
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Re: Never not starting from agar again... [Re: Inocuole]
    #23385081 - 06/26/16 06:42 PM (7 years, 7 months ago)

Gotta lay off the benzos, I totally missed 234 ha


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleInocuole
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Re: Never not starting from agar again... [Re: maddchef]
    #23385091 - 06/26/16 06:43 PM (7 years, 7 months ago)

:rofl: I didn't even see that you labeled it in the subject.  That's gold..


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleLadysKnight
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Re: Never not starting from agar again... [Re: maddchef]
    #23385932 - 06/26/16 10:17 PM (7 years, 7 months ago)

Quote:

maddchef said:
Heh try failing for years on end about 50% of the time and being too damned hardheaded and or stupid to figure it out.




...or 90%


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